A Comprehensive LC-DAD-QTOF-MS Method for Dereplication of Bioactive Compounds in Senna Extracts
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s43450-021-00137-6 http://hdl.handle.net/11449/210704 |
Resumo: | A reversed-phase analytical method was developed and validated by using a combination of high-performance liquid chromatography with diode array detection and ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry systems to dereplicate 46 compounds in samples prepared by mixing extracts from various organs of different Senna species, Fabaceae. The pre-treatment of the sample by solid phase extraction was also investigated. The results obtained showed that the clean-up of the plant extract produces relevant changes in its chemical profile, resulting in false fingerprints. The efficiency of the method was applying to compare the chemical profile of the extracts of seven different Senna species: S. cearensis Afr. Fern, S. macranthera (Collad.) H.S.Irwin & Bameby, S. obtusffolia (L.) H.S.Irwin & Bameby, S. reticulata (Willd.) H.S.Irwin & Barneby, S. spectabilis var. excelsa (DC.) H.S.Irwin & Barneby, S. splendida (Vogel) H.S.Irwin & Barneby, and S. trachypus (Benth.) H.S.Irwin & Barneby. These results suggest that the comprehensive developed method can be applied to the study and dereplication of the chemical composition of other Senna species, preferentially without clean-up of the samples. |
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A Comprehensive LC-DAD-QTOF-MS Method for Dereplication of Bioactive Compounds in Senna ExtractsDereplicationExtract pre-treatmentLiquid chromatographyMethod optimizationSennosidesA reversed-phase analytical method was developed and validated by using a combination of high-performance liquid chromatography with diode array detection and ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry systems to dereplicate 46 compounds in samples prepared by mixing extracts from various organs of different Senna species, Fabaceae. The pre-treatment of the sample by solid phase extraction was also investigated. The results obtained showed that the clean-up of the plant extract produces relevant changes in its chemical profile, resulting in false fingerprints. The efficiency of the method was applying to compare the chemical profile of the extracts of seven different Senna species: S. cearensis Afr. Fern, S. macranthera (Collad.) H.S.Irwin & Bameby, S. obtusffolia (L.) H.S.Irwin & Bameby, S. reticulata (Willd.) H.S.Irwin & Barneby, S. spectabilis var. excelsa (DC.) H.S.Irwin & Barneby, S. splendida (Vogel) H.S.Irwin & Barneby, and S. trachypus (Benth.) H.S.Irwin & Barneby. These results suggest that the comprehensive developed method can be applied to the study and dereplication of the chemical composition of other Senna species, preferentially without clean-up of the samples.Instituto Nacional de Ciencia e Tecnologia em Biodiversidade e Produtos Naturais (INCT BioNat)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Ceara, Dept Quim Organ & Inorgan, Dept Quim Analit & Fis Quim, BR-60455970 Fortaleza, Ceara, BrazilUniv Estadual Paulista, Inst Quim, BR-14800060 Araraquara, SP, BrazilUniv Estadual Paulista, Inst Quim, BR-14800060 Araraquara, SP, BrazilFAPESP: 2013/07600-3: CNPq 465637/2014-0/FAPESP 2014/50926-0SpringerUniv Fed CearaUniversidade Estadual Paulista (Unesp)Franca, Maria G. A.Cavalheiro, Alberto J. [UNESP]Silva, Maria G. V.2021-06-26T02:54:28Z2021-06-26T02:54:28Z2021-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article32-39http://dx.doi.org/10.1007/s43450-021-00137-6Revista Brasileira De Farmacognosia-brazilian Journal Of Pharmacognosy. London: Springernature, v. 31, n. 1, p. 32-39, 2021.0102-695Xhttp://hdl.handle.net/11449/21070410.1007/s43450-021-00137-6WOS:000623821000001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira De Farmacognosia-brazilian Journal Of Pharmacognosyinfo:eu-repo/semantics/openAccess2021-10-23T22:13:49Zoai:repositorio.unesp.br:11449/210704Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:53:58.595211Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A Comprehensive LC-DAD-QTOF-MS Method for Dereplication of Bioactive Compounds in Senna Extracts |
title |
A Comprehensive LC-DAD-QTOF-MS Method for Dereplication of Bioactive Compounds in Senna Extracts |
spellingShingle |
A Comprehensive LC-DAD-QTOF-MS Method for Dereplication of Bioactive Compounds in Senna Extracts Franca, Maria G. A. Dereplication Extract pre-treatment Liquid chromatography Method optimization Sennosides |
title_short |
A Comprehensive LC-DAD-QTOF-MS Method for Dereplication of Bioactive Compounds in Senna Extracts |
title_full |
A Comprehensive LC-DAD-QTOF-MS Method for Dereplication of Bioactive Compounds in Senna Extracts |
title_fullStr |
A Comprehensive LC-DAD-QTOF-MS Method for Dereplication of Bioactive Compounds in Senna Extracts |
title_full_unstemmed |
A Comprehensive LC-DAD-QTOF-MS Method for Dereplication of Bioactive Compounds in Senna Extracts |
title_sort |
A Comprehensive LC-DAD-QTOF-MS Method for Dereplication of Bioactive Compounds in Senna Extracts |
author |
Franca, Maria G. A. |
author_facet |
Franca, Maria G. A. Cavalheiro, Alberto J. [UNESP] Silva, Maria G. V. |
author_role |
author |
author2 |
Cavalheiro, Alberto J. [UNESP] Silva, Maria G. V. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Univ Fed Ceara Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Franca, Maria G. A. Cavalheiro, Alberto J. [UNESP] Silva, Maria G. V. |
dc.subject.por.fl_str_mv |
Dereplication Extract pre-treatment Liquid chromatography Method optimization Sennosides |
topic |
Dereplication Extract pre-treatment Liquid chromatography Method optimization Sennosides |
description |
A reversed-phase analytical method was developed and validated by using a combination of high-performance liquid chromatography with diode array detection and ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry systems to dereplicate 46 compounds in samples prepared by mixing extracts from various organs of different Senna species, Fabaceae. The pre-treatment of the sample by solid phase extraction was also investigated. The results obtained showed that the clean-up of the plant extract produces relevant changes in its chemical profile, resulting in false fingerprints. The efficiency of the method was applying to compare the chemical profile of the extracts of seven different Senna species: S. cearensis Afr. Fern, S. macranthera (Collad.) H.S.Irwin & Bameby, S. obtusffolia (L.) H.S.Irwin & Bameby, S. reticulata (Willd.) H.S.Irwin & Barneby, S. spectabilis var. excelsa (DC.) H.S.Irwin & Barneby, S. splendida (Vogel) H.S.Irwin & Barneby, and S. trachypus (Benth.) H.S.Irwin & Barneby. These results suggest that the comprehensive developed method can be applied to the study and dereplication of the chemical composition of other Senna species, preferentially without clean-up of the samples. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-26T02:54:28Z 2021-06-26T02:54:28Z 2021-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s43450-021-00137-6 Revista Brasileira De Farmacognosia-brazilian Journal Of Pharmacognosy. London: Springernature, v. 31, n. 1, p. 32-39, 2021. 0102-695X http://hdl.handle.net/11449/210704 10.1007/s43450-021-00137-6 WOS:000623821000001 |
url |
http://dx.doi.org/10.1007/s43450-021-00137-6 http://hdl.handle.net/11449/210704 |
identifier_str_mv |
Revista Brasileira De Farmacognosia-brazilian Journal Of Pharmacognosy. London: Springernature, v. 31, n. 1, p. 32-39, 2021. 0102-695X 10.1007/s43450-021-00137-6 WOS:000623821000001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira De Farmacognosia-brazilian Journal Of Pharmacognosy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
32-39 |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129261823852544 |