Bacteria-derived hydrogen sulfide promotes IL-8 production from epithelial cells
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bbrc.2009.11.113 http://hdl.handle.net/11449/15986 |
Resumo: | Hydrogen Sulfide (H(2)S) a volatile Sulfur compound, is implicated as a cause of inflammation. especially when it is produced by bacteria colonizing gastrointestinal organs However, It IS Unclear if H(2)S produced by periodontal pathogens affects the inflammatory responses mediated by oral/gingival epithelial cells Therefore. the aims of this Study were (1) to compare the in vitro production of H(2)S among. 14 strains of Oral bacteria and (2) to evaluate the effects of H(2)S on inflammatory response induced in host oral/gingival epithelial cells Porphyromonas gingivalis (Pg) produced the most H(2)S in Culture, Which, in turn resulted in the promotion of proinflammatory cytokine IL-8 from both gingival and Oral epithelial cells The up-regulation of IL-8 expression was reproduced by the exogenously applied H(2)S Furthermore. the Mutant Strains of Pg that do not produce major Soluble Virulent factors. ie gingival, still showed the Production of H(2)S. as well as the promotion of epithelial IL-8 production. which was abrogated by H(2)S scavenging reagents These results demonstrated that Pg produces a concentration of H(2)S capable of Up-regulating-IL-8 expression induced in gingival and oral epithelial cells, revealing a possible mechanism that may promote the inflammation in periodontal disease (C) 2009 Elsevier B.V. All rights reserved |
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Bacteria-derived hydrogen sulfide promotes IL-8 production from epithelial cellsHydrogen sulfideEpithelial cellsIL-8BacteriaPorphyromonas gingivalisGingipainPathogenesisHydrogenInnate immune responsePeriodontal diseaseHydrogen Sulfide (H(2)S) a volatile Sulfur compound, is implicated as a cause of inflammation. especially when it is produced by bacteria colonizing gastrointestinal organs However, It IS Unclear if H(2)S produced by periodontal pathogens affects the inflammatory responses mediated by oral/gingival epithelial cells Therefore. the aims of this Study were (1) to compare the in vitro production of H(2)S among. 14 strains of Oral bacteria and (2) to evaluate the effects of H(2)S on inflammatory response induced in host oral/gingival epithelial cells Porphyromonas gingivalis (Pg) produced the most H(2)S in Culture, Which, in turn resulted in the promotion of proinflammatory cytokine IL-8 from both gingival and Oral epithelial cells The up-regulation of IL-8 expression was reproduced by the exogenously applied H(2)S Furthermore. the Mutant Strains of Pg that do not produce major Soluble Virulent factors. ie gingival, still showed the Production of H(2)S. as well as the promotion of epithelial IL-8 production. which was abrogated by H(2)S scavenging reagents These results demonstrated that Pg produces a concentration of H(2)S capable of Up-regulating-IL-8 expression induced in gingival and oral epithelial cells, revealing a possible mechanism that may promote the inflammation in periodontal disease (C) 2009 Elsevier B.V. All rights reservedSkyview EnterprisesNIHHoward Hughes Medical InstituteForsyth Inst, Dept Immunol, Boston, MA 02115 USAForsyth Inst, Dept Mol Genet, Boston, MA 02115 USAUNESP, Dept Oral Diag & Surg, Sch Dent Araraquara, Jaboticabal, SP, BrazilSkyview Enterprises, New York, NY USAUNESP, Dept Oral Diag & Surg, Sch Dent Araraquara, Jaboticabal, SP, BrazilNIH: DE-018310NIH: DE-015931Academic Press Inc. Elsevier B.V.Forsyth InstUniversidade Estadual Paulista (Unesp)Skyview EnterprisesChen, WeilinKajiya, MikihitoGiro, Gabriela [UNESP]Ouhara, KazuhisaMackler, Harrison E.Mawardi, HaniBoisvert, HeikeDuncan, Margaret J.Sato, KimihiroKawai, Toshihisa2013-09-30T18:31:53Z2014-05-20T13:45:26Z2013-09-30T18:31:53Z2014-05-20T13:45:26Z2010-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject645-650http://dx.doi.org/10.1016/j.bbrc.2009.11.113Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc. Elsevier B.V., v. 391, n. 1, p. 645-650, 2010.0006-291Xhttp://hdl.handle.net/11449/1598610.1016/j.bbrc.2009.11.113WOS:000273624500113Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiochemical and Biophysical Research Communications2.559info:eu-repo/semantics/openAccess2021-10-23T21:37:52Zoai:repositorio.unesp.br:11449/15986Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T21:37:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bacteria-derived hydrogen sulfide promotes IL-8 production from epithelial cells |
title |
Bacteria-derived hydrogen sulfide promotes IL-8 production from epithelial cells |
spellingShingle |
Bacteria-derived hydrogen sulfide promotes IL-8 production from epithelial cells Chen, Weilin Hydrogen sulfide Epithelial cells IL-8 Bacteria Porphyromonas gingivalis Gingipain Pathogenesis Hydrogen Innate immune response Periodontal disease |
title_short |
Bacteria-derived hydrogen sulfide promotes IL-8 production from epithelial cells |
title_full |
Bacteria-derived hydrogen sulfide promotes IL-8 production from epithelial cells |
title_fullStr |
Bacteria-derived hydrogen sulfide promotes IL-8 production from epithelial cells |
title_full_unstemmed |
Bacteria-derived hydrogen sulfide promotes IL-8 production from epithelial cells |
title_sort |
Bacteria-derived hydrogen sulfide promotes IL-8 production from epithelial cells |
author |
Chen, Weilin |
author_facet |
Chen, Weilin Kajiya, Mikihito Giro, Gabriela [UNESP] Ouhara, Kazuhisa Mackler, Harrison E. Mawardi, Hani Boisvert, Heike Duncan, Margaret J. Sato, Kimihiro Kawai, Toshihisa |
author_role |
author |
author2 |
Kajiya, Mikihito Giro, Gabriela [UNESP] Ouhara, Kazuhisa Mackler, Harrison E. Mawardi, Hani Boisvert, Heike Duncan, Margaret J. Sato, Kimihiro Kawai, Toshihisa |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Forsyth Inst Universidade Estadual Paulista (Unesp) Skyview Enterprises |
dc.contributor.author.fl_str_mv |
Chen, Weilin Kajiya, Mikihito Giro, Gabriela [UNESP] Ouhara, Kazuhisa Mackler, Harrison E. Mawardi, Hani Boisvert, Heike Duncan, Margaret J. Sato, Kimihiro Kawai, Toshihisa |
dc.subject.por.fl_str_mv |
Hydrogen sulfide Epithelial cells IL-8 Bacteria Porphyromonas gingivalis Gingipain Pathogenesis Hydrogen Innate immune response Periodontal disease |
topic |
Hydrogen sulfide Epithelial cells IL-8 Bacteria Porphyromonas gingivalis Gingipain Pathogenesis Hydrogen Innate immune response Periodontal disease |
description |
Hydrogen Sulfide (H(2)S) a volatile Sulfur compound, is implicated as a cause of inflammation. especially when it is produced by bacteria colonizing gastrointestinal organs However, It IS Unclear if H(2)S produced by periodontal pathogens affects the inflammatory responses mediated by oral/gingival epithelial cells Therefore. the aims of this Study were (1) to compare the in vitro production of H(2)S among. 14 strains of Oral bacteria and (2) to evaluate the effects of H(2)S on inflammatory response induced in host oral/gingival epithelial cells Porphyromonas gingivalis (Pg) produced the most H(2)S in Culture, Which, in turn resulted in the promotion of proinflammatory cytokine IL-8 from both gingival and Oral epithelial cells The up-regulation of IL-8 expression was reproduced by the exogenously applied H(2)S Furthermore. the Mutant Strains of Pg that do not produce major Soluble Virulent factors. ie gingival, still showed the Production of H(2)S. as well as the promotion of epithelial IL-8 production. which was abrogated by H(2)S scavenging reagents These results demonstrated that Pg produces a concentration of H(2)S capable of Up-regulating-IL-8 expression induced in gingival and oral epithelial cells, revealing a possible mechanism that may promote the inflammation in periodontal disease (C) 2009 Elsevier B.V. All rights reserved |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 2013-09-30T18:31:53Z 2013-09-30T18:31:53Z 2014-05-20T13:45:26Z 2014-05-20T13:45:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bbrc.2009.11.113 Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc. Elsevier B.V., v. 391, n. 1, p. 645-650, 2010. 0006-291X http://hdl.handle.net/11449/15986 10.1016/j.bbrc.2009.11.113 WOS:000273624500113 |
url |
http://dx.doi.org/10.1016/j.bbrc.2009.11.113 http://hdl.handle.net/11449/15986 |
identifier_str_mv |
Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc. Elsevier B.V., v. 391, n. 1, p. 645-650, 2010. 0006-291X 10.1016/j.bbrc.2009.11.113 WOS:000273624500113 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biochemical and Biophysical Research Communications 2.559 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
645-650 |
dc.publisher.none.fl_str_mv |
Academic Press Inc. Elsevier B.V. |
publisher.none.fl_str_mv |
Academic Press Inc. Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1803046558300635136 |