Variation of calcium, copper and iron levels in serum, bile and stone samples of patients having different types of gallstone: A comparative study

Detalhes bibliográficos
Autor(a) principal: Khan, Mustafa
Data de Publicação: 2017
Outros Autores: Kazi, Tasneem Gul, Afridi, Hassan Imran, Sirajuddin, Bilal, Muhammad, Akhtar, Asma, Khan, Sabir [UNESP], Kadar, Salma
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.cca.2017.06.012
http://hdl.handle.net/11449/174788
Resumo: Background Epidemiological data among the human population has shown a significantly increased incidence of gallstone (GS) disease worldwide. It was studied that some essential (calcium) and transition elements (iron and copper) in bile play an important role in the development of GS. Method The estimation of calcium, copper and iron were carried out in the serum, gall bladder bile and different types of GS (cholesterol, mixed and pigmented) of 172 patients, age ranged 20–55 years. For comparative purpose age matched referents not suffering from GS diseases were also selected. Biliary concentrations of calcium (Ca), iron (Fe) and copper (Cu) were correlated with their concentrations in serum and different types of GS samples. The ratio of Ca, Fe and Cu in bile with serum was also calculated. Understudy metals were determined by flame atomic absorption spectroscopy after acid decomposition of matrices of selected samples. Results The Ca concentrations in serum samples were significantly higher in patients with pigmented GS as compared to controls (p < 0.005), whereas for patients having cholesterol and mixed GS the concentrations were on the lower side. Biliary Ca concentrations of patients were found to be higher than controls, but difference was significant for pigmented GS patients (p > 0.001). The contents of Cu and Fe in serum and bile of all patients (except female cholesterol GS patient have low serum iron concentration) were found to be higher than control, but difference was significant in those patients who have pigmented GS. The concentration of Ca, Fe and Cu in different types GS were found in the order, Pigmented > mixed > cholesterol. The bile/serum ratio for Ca, Cu and Fe was found to be significantly higher in pigmented GS patients. Gall bladder bile was slightly alkaline in patients as compared to referents. The density of bile was found to be higher in patients as compared to the referents. Various functional groups present in different types of GS samples were confirmed by Fourier transform infra-red spectroscopy. Conclusion The higher density and pH of bile, elevated concentrations of transition elements in all types of biological samples (serum, bile and GS), could be an important factor for the formation of different types of GS.
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spelling Variation of calcium, copper and iron levels in serum, bile and stone samples of patients having different types of gallstone: A comparative studyBileCalciumCopperDifferent types of gallstoneIronSerumBackground Epidemiological data among the human population has shown a significantly increased incidence of gallstone (GS) disease worldwide. It was studied that some essential (calcium) and transition elements (iron and copper) in bile play an important role in the development of GS. Method The estimation of calcium, copper and iron were carried out in the serum, gall bladder bile and different types of GS (cholesterol, mixed and pigmented) of 172 patients, age ranged 20–55 years. For comparative purpose age matched referents not suffering from GS diseases were also selected. Biliary concentrations of calcium (Ca), iron (Fe) and copper (Cu) were correlated with their concentrations in serum and different types of GS samples. The ratio of Ca, Fe and Cu in bile with serum was also calculated. Understudy metals were determined by flame atomic absorption spectroscopy after acid decomposition of matrices of selected samples. Results The Ca concentrations in serum samples were significantly higher in patients with pigmented GS as compared to controls (p < 0.005), whereas for patients having cholesterol and mixed GS the concentrations were on the lower side. Biliary Ca concentrations of patients were found to be higher than controls, but difference was significant for pigmented GS patients (p > 0.001). The contents of Cu and Fe in serum and bile of all patients (except female cholesterol GS patient have low serum iron concentration) were found to be higher than control, but difference was significant in those patients who have pigmented GS. The concentration of Ca, Fe and Cu in different types GS were found in the order, Pigmented > mixed > cholesterol. The bile/serum ratio for Ca, Cu and Fe was found to be significantly higher in pigmented GS patients. Gall bladder bile was slightly alkaline in patients as compared to referents. The density of bile was found to be higher in patients as compared to the referents. Various functional groups present in different types of GS samples were confirmed by Fourier transform infra-red spectroscopy. Conclusion The higher density and pH of bile, elevated concentrations of transition elements in all types of biological samples (serum, bile and GS), could be an important factor for the formation of different types of GS.National Centre of Excellence in Analytical Chemistry University of SindhUniversidade Estadual Paulista (UNESP) Department of Analytical Chemistry Institute of Chemistry, Rua Professor Francisco Degni, 55, QuitandinhaNeuro Diagnostic Centre Liaquat University of Medical & Health SciencesUniversidade Estadual Paulista (UNESP) Department of Analytical Chemistry Institute of Chemistry, Rua Professor Francisco Degni, 55, QuitandinhaUniversity of SindhUniversidade Estadual Paulista (Unesp)Liaquat University of Medical & Health SciencesKhan, MustafaKazi, Tasneem GulAfridi, Hassan ImranSirajuddin,Bilal, MuhammadAkhtar, AsmaKhan, Sabir [UNESP]Kadar, Salma2018-12-11T17:12:52Z2018-12-11T17:12:52Z2017-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article254-262application/pdfhttp://dx.doi.org/10.1016/j.cca.2017.06.012Clinica Chimica Acta, v. 471, p. 254-262.1873-34920009-8981http://hdl.handle.net/11449/17478810.1016/j.cca.2017.06.0122-s2.0-850211217202-s2.0-85021121720.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinica Chimica Acta1,102info:eu-repo/semantics/openAccess2023-12-31T06:16:45Zoai:repositorio.unesp.br:11449/174788Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:45:16.518411Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Variation of calcium, copper and iron levels in serum, bile and stone samples of patients having different types of gallstone: A comparative study
title Variation of calcium, copper and iron levels in serum, bile and stone samples of patients having different types of gallstone: A comparative study
spellingShingle Variation of calcium, copper and iron levels in serum, bile and stone samples of patients having different types of gallstone: A comparative study
Khan, Mustafa
Bile
Calcium
Copper
Different types of gallstone
Iron
Serum
title_short Variation of calcium, copper and iron levels in serum, bile and stone samples of patients having different types of gallstone: A comparative study
title_full Variation of calcium, copper and iron levels in serum, bile and stone samples of patients having different types of gallstone: A comparative study
title_fullStr Variation of calcium, copper and iron levels in serum, bile and stone samples of patients having different types of gallstone: A comparative study
title_full_unstemmed Variation of calcium, copper and iron levels in serum, bile and stone samples of patients having different types of gallstone: A comparative study
title_sort Variation of calcium, copper and iron levels in serum, bile and stone samples of patients having different types of gallstone: A comparative study
author Khan, Mustafa
author_facet Khan, Mustafa
Kazi, Tasneem Gul
Afridi, Hassan Imran
Sirajuddin,
Bilal, Muhammad
Akhtar, Asma
Khan, Sabir [UNESP]
Kadar, Salma
author_role author
author2 Kazi, Tasneem Gul
Afridi, Hassan Imran
Sirajuddin,
Bilal, Muhammad
Akhtar, Asma
Khan, Sabir [UNESP]
Kadar, Salma
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv University of Sindh
Universidade Estadual Paulista (Unesp)
Liaquat University of Medical & Health Sciences
dc.contributor.author.fl_str_mv Khan, Mustafa
Kazi, Tasneem Gul
Afridi, Hassan Imran
Sirajuddin,
Bilal, Muhammad
Akhtar, Asma
Khan, Sabir [UNESP]
Kadar, Salma
dc.subject.por.fl_str_mv Bile
Calcium
Copper
Different types of gallstone
Iron
Serum
topic Bile
Calcium
Copper
Different types of gallstone
Iron
Serum
description Background Epidemiological data among the human population has shown a significantly increased incidence of gallstone (GS) disease worldwide. It was studied that some essential (calcium) and transition elements (iron and copper) in bile play an important role in the development of GS. Method The estimation of calcium, copper and iron were carried out in the serum, gall bladder bile and different types of GS (cholesterol, mixed and pigmented) of 172 patients, age ranged 20–55 years. For comparative purpose age matched referents not suffering from GS diseases were also selected. Biliary concentrations of calcium (Ca), iron (Fe) and copper (Cu) were correlated with their concentrations in serum and different types of GS samples. The ratio of Ca, Fe and Cu in bile with serum was also calculated. Understudy metals were determined by flame atomic absorption spectroscopy after acid decomposition of matrices of selected samples. Results The Ca concentrations in serum samples were significantly higher in patients with pigmented GS as compared to controls (p < 0.005), whereas for patients having cholesterol and mixed GS the concentrations were on the lower side. Biliary Ca concentrations of patients were found to be higher than controls, but difference was significant for pigmented GS patients (p > 0.001). The contents of Cu and Fe in serum and bile of all patients (except female cholesterol GS patient have low serum iron concentration) were found to be higher than control, but difference was significant in those patients who have pigmented GS. The concentration of Ca, Fe and Cu in different types GS were found in the order, Pigmented > mixed > cholesterol. The bile/serum ratio for Ca, Cu and Fe was found to be significantly higher in pigmented GS patients. Gall bladder bile was slightly alkaline in patients as compared to referents. The density of bile was found to be higher in patients as compared to the referents. Various functional groups present in different types of GS samples were confirmed by Fourier transform infra-red spectroscopy. Conclusion The higher density and pH of bile, elevated concentrations of transition elements in all types of biological samples (serum, bile and GS), could be an important factor for the formation of different types of GS.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-01
2018-12-11T17:12:52Z
2018-12-11T17:12:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.cca.2017.06.012
Clinica Chimica Acta, v. 471, p. 254-262.
1873-3492
0009-8981
http://hdl.handle.net/11449/174788
10.1016/j.cca.2017.06.012
2-s2.0-85021121720
2-s2.0-85021121720.pdf
url http://dx.doi.org/10.1016/j.cca.2017.06.012
http://hdl.handle.net/11449/174788
identifier_str_mv Clinica Chimica Acta, v. 471, p. 254-262.
1873-3492
0009-8981
10.1016/j.cca.2017.06.012
2-s2.0-85021121720
2-s2.0-85021121720.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinica Chimica Acta
1,102
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 254-262
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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