Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp Regeneration
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.joen.2021.04.009 http://hdl.handle.net/11449/207759 |
Resumo: | Introduction: Guided tissue regeneration has been considered a promising biological strategy to replace conventional endodontic therapies of teeth with incomplete root formation. Therefore, in the present study, a collagen/gelatin hydrogel either containing dosages of fibronectin (FN), or not, was developed and assessed concerning their bioactive and chemotactic potential on human apical papilla cells (hAPCs). Methods: Hydrogels were prepared by varying the ratio of collagen and gelatin (Col/Gel; v/v), and used to establish the following groups: Collagen (positive control); Col/Gel 4:6; Col/Gel 6:4; Col/Gel 8:2. The viability, adhesion, and spreading of cells seeded on the hydrogels were evaluated. Different concentrations of FN (0, 5, or 10 μg/mL) were incorporated into the best formulation of the collagen/gelatin hydrogel selected. Then, the hAPCs seeded on the biomaterials were assessed concerning the cell migration, viability, adhesion and spreading, and gene expression of ITGA5, ITGAV, COL1A1, and COL3A1. Results: The Col/Gel 8:2 group exhibited better cell viability, adhesion and spreading in comparison with Control. Higher values of hAPC migration, viability, adhesion, spreading and gene expression of pulp regeneration markers were found, the higher the concentration was of FN incorporated into the collagen/gelatin hydrogel. Conclusion: Collagen/gelatin hydrogel with 10 μg/mL of FN had potent bioactive and chemotactic effects on cultured hAPCs. |
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Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp RegenerationcollagenFibronectingelatinHydrogelpulp regenerationIntroduction: Guided tissue regeneration has been considered a promising biological strategy to replace conventional endodontic therapies of teeth with incomplete root formation. Therefore, in the present study, a collagen/gelatin hydrogel either containing dosages of fibronectin (FN), or not, was developed and assessed concerning their bioactive and chemotactic potential on human apical papilla cells (hAPCs). Methods: Hydrogels were prepared by varying the ratio of collagen and gelatin (Col/Gel; v/v), and used to establish the following groups: Collagen (positive control); Col/Gel 4:6; Col/Gel 6:4; Col/Gel 8:2. The viability, adhesion, and spreading of cells seeded on the hydrogels were evaluated. Different concentrations of FN (0, 5, or 10 μg/mL) were incorporated into the best formulation of the collagen/gelatin hydrogel selected. Then, the hAPCs seeded on the biomaterials were assessed concerning the cell migration, viability, adhesion and spreading, and gene expression of ITGA5, ITGAV, COL1A1, and COL3A1. Results: The Col/Gel 8:2 group exhibited better cell viability, adhesion and spreading in comparison with Control. Higher values of hAPC migration, viability, adhesion, spreading and gene expression of pulp regeneration markers were found, the higher the concentration was of FN incorporated into the collagen/gelatin hydrogel. Conclusion: Collagen/gelatin hydrogel with 10 μg/mL of FN had potent bioactive and chemotactic effects on cultured hAPCs.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Dental Materials and Prosthodontics Araraquara School of Dentistry Univ. Estadual Paulista – UNESPDepartment of Operative Dentistry Endodontics and Dental Materials Bauru School of Dentistry University of São PauloDepartament of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry São Paulo State University (Unesp)Department of Physiology and Pathology Araraquara School of Dentistry São Paulo State University (Unesp)Department of Dental Materials and Prosthodontics Araraquara School of Dentistry Univ. Estadual Paulista – UNESPDepartament of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry São Paulo State University (Unesp)Department of Physiology and Pathology Araraquara School of Dentistry São Paulo State University (Unesp)CNPq: 302047/2019–0CNPq: 408721/2018–9Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Leite, Maria Luísa [UNESP]Soares, Diana GabrielaAnovazzi, Giovana [UNESP]Anselmi, Caroline [UNESP]Hebling, Josimeri [UNESP]de Souza Costa, Carlos Alberto [UNESP]2021-06-25T11:00:33Z2021-06-25T11:00:33Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.joen.2021.04.009Journal of Endodontics.0099-2399http://hdl.handle.net/11449/20775910.1016/j.joen.2021.04.0092-s2.0-85106305614Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Endodonticsinfo:eu-repo/semantics/openAccess2024-09-27T14:56:40Zoai:repositorio.unesp.br:11449/207759Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:56:40Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp Regeneration |
title |
Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp Regeneration |
spellingShingle |
Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp Regeneration Leite, Maria Luísa [UNESP] collagen Fibronectin gelatin Hydrogel pulp regeneration |
title_short |
Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp Regeneration |
title_full |
Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp Regeneration |
title_fullStr |
Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp Regeneration |
title_full_unstemmed |
Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp Regeneration |
title_sort |
Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp Regeneration |
author |
Leite, Maria Luísa [UNESP] |
author_facet |
Leite, Maria Luísa [UNESP] Soares, Diana Gabriela Anovazzi, Giovana [UNESP] Anselmi, Caroline [UNESP] Hebling, Josimeri [UNESP] de Souza Costa, Carlos Alberto [UNESP] |
author_role |
author |
author2 |
Soares, Diana Gabriela Anovazzi, Giovana [UNESP] Anselmi, Caroline [UNESP] Hebling, Josimeri [UNESP] de Souza Costa, Carlos Alberto [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Leite, Maria Luísa [UNESP] Soares, Diana Gabriela Anovazzi, Giovana [UNESP] Anselmi, Caroline [UNESP] Hebling, Josimeri [UNESP] de Souza Costa, Carlos Alberto [UNESP] |
dc.subject.por.fl_str_mv |
collagen Fibronectin gelatin Hydrogel pulp regeneration |
topic |
collagen Fibronectin gelatin Hydrogel pulp regeneration |
description |
Introduction: Guided tissue regeneration has been considered a promising biological strategy to replace conventional endodontic therapies of teeth with incomplete root formation. Therefore, in the present study, a collagen/gelatin hydrogel either containing dosages of fibronectin (FN), or not, was developed and assessed concerning their bioactive and chemotactic potential on human apical papilla cells (hAPCs). Methods: Hydrogels were prepared by varying the ratio of collagen and gelatin (Col/Gel; v/v), and used to establish the following groups: Collagen (positive control); Col/Gel 4:6; Col/Gel 6:4; Col/Gel 8:2. The viability, adhesion, and spreading of cells seeded on the hydrogels were evaluated. Different concentrations of FN (0, 5, or 10 μg/mL) were incorporated into the best formulation of the collagen/gelatin hydrogel selected. Then, the hAPCs seeded on the biomaterials were assessed concerning the cell migration, viability, adhesion and spreading, and gene expression of ITGA5, ITGAV, COL1A1, and COL3A1. Results: The Col/Gel 8:2 group exhibited better cell viability, adhesion and spreading in comparison with Control. Higher values of hAPC migration, viability, adhesion, spreading and gene expression of pulp regeneration markers were found, the higher the concentration was of FN incorporated into the collagen/gelatin hydrogel. Conclusion: Collagen/gelatin hydrogel with 10 μg/mL of FN had potent bioactive and chemotactic effects on cultured hAPCs. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:00:33Z 2021-06-25T11:00:33Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.joen.2021.04.009 Journal of Endodontics. 0099-2399 http://hdl.handle.net/11449/207759 10.1016/j.joen.2021.04.009 2-s2.0-85106305614 |
url |
http://dx.doi.org/10.1016/j.joen.2021.04.009 http://hdl.handle.net/11449/207759 |
identifier_str_mv |
Journal of Endodontics. 0099-2399 10.1016/j.joen.2021.04.009 2-s2.0-85106305614 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Endodontics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546399526027264 |