Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp Regeneration

Detalhes bibliográficos
Autor(a) principal: Leite, Maria Luísa [UNESP]
Data de Publicação: 2021
Outros Autores: Soares, Diana Gabriela, Anovazzi, Giovana [UNESP], Anselmi, Caroline [UNESP], Hebling, Josimeri [UNESP], de Souza Costa, Carlos Alberto [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.joen.2021.04.009
http://hdl.handle.net/11449/207759
Resumo: Introduction: Guided tissue regeneration has been considered a promising biological strategy to replace conventional endodontic therapies of teeth with incomplete root formation. Therefore, in the present study, a collagen/gelatin hydrogel either containing dosages of fibronectin (FN), or not, was developed and assessed concerning their bioactive and chemotactic potential on human apical papilla cells (hAPCs). Methods: Hydrogels were prepared by varying the ratio of collagen and gelatin (Col/Gel; v/v), and used to establish the following groups: Collagen (positive control); Col/Gel 4:6; Col/Gel 6:4; Col/Gel 8:2. The viability, adhesion, and spreading of cells seeded on the hydrogels were evaluated. Different concentrations of FN (0, 5, or 10 μg/mL) were incorporated into the best formulation of the collagen/gelatin hydrogel selected. Then, the hAPCs seeded on the biomaterials were assessed concerning the cell migration, viability, adhesion and spreading, and gene expression of ITGA5, ITGAV, COL1A1, and COL3A1. Results: The Col/Gel 8:2 group exhibited better cell viability, adhesion and spreading in comparison with Control. Higher values of hAPC migration, viability, adhesion, spreading and gene expression of pulp regeneration markers were found, the higher the concentration was of FN incorporated into the collagen/gelatin hydrogel. Conclusion: Collagen/gelatin hydrogel with 10 μg/mL of FN had potent bioactive and chemotactic effects on cultured hAPCs.
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spelling Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp RegenerationcollagenFibronectingelatinHydrogelpulp regenerationIntroduction: Guided tissue regeneration has been considered a promising biological strategy to replace conventional endodontic therapies of teeth with incomplete root formation. Therefore, in the present study, a collagen/gelatin hydrogel either containing dosages of fibronectin (FN), or not, was developed and assessed concerning their bioactive and chemotactic potential on human apical papilla cells (hAPCs). Methods: Hydrogels were prepared by varying the ratio of collagen and gelatin (Col/Gel; v/v), and used to establish the following groups: Collagen (positive control); Col/Gel 4:6; Col/Gel 6:4; Col/Gel 8:2. The viability, adhesion, and spreading of cells seeded on the hydrogels were evaluated. Different concentrations of FN (0, 5, or 10 μg/mL) were incorporated into the best formulation of the collagen/gelatin hydrogel selected. Then, the hAPCs seeded on the biomaterials were assessed concerning the cell migration, viability, adhesion and spreading, and gene expression of ITGA5, ITGAV, COL1A1, and COL3A1. Results: The Col/Gel 8:2 group exhibited better cell viability, adhesion and spreading in comparison with Control. Higher values of hAPC migration, viability, adhesion, spreading and gene expression of pulp regeneration markers were found, the higher the concentration was of FN incorporated into the collagen/gelatin hydrogel. Conclusion: Collagen/gelatin hydrogel with 10 μg/mL of FN had potent bioactive and chemotactic effects on cultured hAPCs.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Dental Materials and Prosthodontics Araraquara School of Dentistry Univ. Estadual Paulista – UNESPDepartment of Operative Dentistry Endodontics and Dental Materials Bauru School of Dentistry University of São PauloDepartament of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry São Paulo State University (Unesp)Department of Physiology and Pathology Araraquara School of Dentistry São Paulo State University (Unesp)Department of Dental Materials and Prosthodontics Araraquara School of Dentistry Univ. Estadual Paulista – UNESPDepartament of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry São Paulo State University (Unesp)Department of Physiology and Pathology Araraquara School of Dentistry São Paulo State University (Unesp)CNPq: 302047/2019–0CNPq: 408721/2018–9Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Leite, Maria Luísa [UNESP]Soares, Diana GabrielaAnovazzi, Giovana [UNESP]Anselmi, Caroline [UNESP]Hebling, Josimeri [UNESP]de Souza Costa, Carlos Alberto [UNESP]2021-06-25T11:00:33Z2021-06-25T11:00:33Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.joen.2021.04.009Journal of Endodontics.0099-2399http://hdl.handle.net/11449/20775910.1016/j.joen.2021.04.0092-s2.0-85106305614Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Endodonticsinfo:eu-repo/semantics/openAccess2024-09-27T14:56:40Zoai:repositorio.unesp.br:11449/207759Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:56:40Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp Regeneration
title Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp Regeneration
spellingShingle Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp Regeneration
Leite, Maria Luísa [UNESP]
collagen
Fibronectin
gelatin
Hydrogel
pulp regeneration
title_short Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp Regeneration
title_full Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp Regeneration
title_fullStr Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp Regeneration
title_full_unstemmed Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp Regeneration
title_sort Fibronectin-loaded Collagen/Gelatin Hydrogel Is a Potent Signaling Biomaterial for Dental Pulp Regeneration
author Leite, Maria Luísa [UNESP]
author_facet Leite, Maria Luísa [UNESP]
Soares, Diana Gabriela
Anovazzi, Giovana [UNESP]
Anselmi, Caroline [UNESP]
Hebling, Josimeri [UNESP]
de Souza Costa, Carlos Alberto [UNESP]
author_role author
author2 Soares, Diana Gabriela
Anovazzi, Giovana [UNESP]
Anselmi, Caroline [UNESP]
Hebling, Josimeri [UNESP]
de Souza Costa, Carlos Alberto [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Leite, Maria Luísa [UNESP]
Soares, Diana Gabriela
Anovazzi, Giovana [UNESP]
Anselmi, Caroline [UNESP]
Hebling, Josimeri [UNESP]
de Souza Costa, Carlos Alberto [UNESP]
dc.subject.por.fl_str_mv collagen
Fibronectin
gelatin
Hydrogel
pulp regeneration
topic collagen
Fibronectin
gelatin
Hydrogel
pulp regeneration
description Introduction: Guided tissue regeneration has been considered a promising biological strategy to replace conventional endodontic therapies of teeth with incomplete root formation. Therefore, in the present study, a collagen/gelatin hydrogel either containing dosages of fibronectin (FN), or not, was developed and assessed concerning their bioactive and chemotactic potential on human apical papilla cells (hAPCs). Methods: Hydrogels were prepared by varying the ratio of collagen and gelatin (Col/Gel; v/v), and used to establish the following groups: Collagen (positive control); Col/Gel 4:6; Col/Gel 6:4; Col/Gel 8:2. The viability, adhesion, and spreading of cells seeded on the hydrogels were evaluated. Different concentrations of FN (0, 5, or 10 μg/mL) were incorporated into the best formulation of the collagen/gelatin hydrogel selected. Then, the hAPCs seeded on the biomaterials were assessed concerning the cell migration, viability, adhesion and spreading, and gene expression of ITGA5, ITGAV, COL1A1, and COL3A1. Results: The Col/Gel 8:2 group exhibited better cell viability, adhesion and spreading in comparison with Control. Higher values of hAPC migration, viability, adhesion, spreading and gene expression of pulp regeneration markers were found, the higher the concentration was of FN incorporated into the collagen/gelatin hydrogel. Conclusion: Collagen/gelatin hydrogel with 10 μg/mL of FN had potent bioactive and chemotactic effects on cultured hAPCs.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T11:00:33Z
2021-06-25T11:00:33Z
2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.joen.2021.04.009
Journal of Endodontics.
0099-2399
http://hdl.handle.net/11449/207759
10.1016/j.joen.2021.04.009
2-s2.0-85106305614
url http://dx.doi.org/10.1016/j.joen.2021.04.009
http://hdl.handle.net/11449/207759
identifier_str_mv Journal of Endodontics.
0099-2399
10.1016/j.joen.2021.04.009
2-s2.0-85106305614
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Endodontics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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