Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) pegler lignocellulolitic activity
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1516-89132008000200001 http://hdl.handle.net/11449/19113 |
Resumo: | This work aimed to study the stationary and periodically mixed culture of L. edodes to the production of lignocellulolitic enzymes activity. LE 95/17, LE 96/22 and Leax strains were incubated in 25 g of eucalyptus sawdust substrate in Erlenmeyer flasks in stationary culture at 25 degrees C and in a bioreactor with four complete rotations daily at 25 degrees C and 3% CO2. The samples were collected at 8, 11, 14, 17 and 20 days after the incubation. Oxidative and hydrolytic enzymes analyses were performed. Lignin peroxidase enzyme was not found in the lignolytic systernfor LE 95/17, LE 96/22 and Leax strains in the different incubation methods. The use of bioreactor could be a practicable system to induce the laccase activity for L22 and Leax and MnP activity for L17 and L22. The activity of the hydrolytic enzymes was higher in the stationary system in comparison to periodically mixed system in the bioreactor. |
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Repositório Institucional da UNESP |
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Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) pegler lignocellulolitic activityshiitakeenzymesagricultural residuesThis work aimed to study the stationary and periodically mixed culture of L. edodes to the production of lignocellulolitic enzymes activity. LE 95/17, LE 96/22 and Leax strains were incubated in 25 g of eucalyptus sawdust substrate in Erlenmeyer flasks in stationary culture at 25 degrees C and in a bioreactor with four complete rotations daily at 25 degrees C and 3% CO2. The samples were collected at 8, 11, 14, 17 and 20 days after the incubation. Oxidative and hydrolytic enzymes analyses were performed. Lignin peroxidase enzyme was not found in the lignolytic systernfor LE 95/17, LE 96/22 and Leax strains in the different incubation methods. The use of bioreactor could be a practicable system to induce the laccase activity for L22 and Leax and MnP activity for L17 and L22. The activity of the hydrolytic enzymes was higher in the stationary system in comparison to periodically mixed system in the bioreactor.Lentinula edodes pertence ao grupo de fungos causadores da podridão branca, conhecidos pela capacidade efetiva de degradar compostos lignocelulósicos. No cultivo deste fungo o método de incubação, substratos e características genéticas determinam o comportamento enzimático. Este trabalho teve como objetivo verificar a influência do cultivo estacionário e em bioreator na atividade de enzimas lignocelulolíticas do L. edodes. As linhagens LE 95/17, LE 96/22 e Leax foram cultivadas em 25 g de substrato composto de serragem de eucalipto em frascos de Erlenmeyer, em cultura estacionária, sob de temperatura controlada a 25° C e em bioreator com quatro rotações diárias e completas, a 25° C e 3% de CO2. Foram coletados dados aos 8, 11, 14, 17 e 20 dias depois da incubação para atividade de enzimas oxidativas e hidrolíticas. A enzima lignina peroxidase não foi encontrada em nenhum dos tratamentos. O uso de bioreator pode ser visto como um sistema praticável para induzir atividade de laccase para L22 e Leax e atividade de MnP para L17 e L22. A atividade de enzimas hidrolíticas foi maior no sistema estacionário em comparação com o sistema misturado periodicamente em bioreator.São Paulo State Univ UNESP, Dept Chem & Biochem, IB, BR-18618000 Botucatu, SP, BrazilUniv Tuscia, Agrobiol & Agrochem Dept, I-01100 Viterbo, ItalySão Paulo State Univ UNESP, Dept Chem & Biochem, IB, BR-18618000 Botucatu, SP, BrazilInst Tecnologia ParanaUniversidade Estadual Paulista (Unesp)Univ TusciaRegina, Magali [UNESP]Broetto, Fernando [UNESP]Giovannozzi-Sermanni, GiovanniMarabotini, RositaPeranni, Claudio2014-05-20T13:53:33Z2014-05-20T13:53:33Z2008-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article223-233application/pdfhttp://dx.doi.org/10.1590/S1516-89132008000200001Brazilian Archives of Biology and Technology. Curitiba-parana: Inst Tecnologia Parana, v. 51, n. 2, p. 223-233, 2008.1516-8913http://hdl.handle.net/11449/19113S1516-89132008000200001WOS:000256701300001S1516-89132008000200001.pdf3964089477817663Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Archives of Biology and Technology0.6760,281info:eu-repo/semantics/openAccess2024-01-27T07:00:25Zoai:repositorio.unesp.br:11449/19113Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-27T07:00:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) pegler lignocellulolitic activity |
title |
Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) pegler lignocellulolitic activity |
spellingShingle |
Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) pegler lignocellulolitic activity Regina, Magali [UNESP] shiitake enzymes agricultural residues |
title_short |
Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) pegler lignocellulolitic activity |
title_full |
Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) pegler lignocellulolitic activity |
title_fullStr |
Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) pegler lignocellulolitic activity |
title_full_unstemmed |
Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) pegler lignocellulolitic activity |
title_sort |
Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) pegler lignocellulolitic activity |
author |
Regina, Magali [UNESP] |
author_facet |
Regina, Magali [UNESP] Broetto, Fernando [UNESP] Giovannozzi-Sermanni, Giovanni Marabotini, Rosita Peranni, Claudio |
author_role |
author |
author2 |
Broetto, Fernando [UNESP] Giovannozzi-Sermanni, Giovanni Marabotini, Rosita Peranni, Claudio |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Tuscia |
dc.contributor.author.fl_str_mv |
Regina, Magali [UNESP] Broetto, Fernando [UNESP] Giovannozzi-Sermanni, Giovanni Marabotini, Rosita Peranni, Claudio |
dc.subject.por.fl_str_mv |
shiitake enzymes agricultural residues |
topic |
shiitake enzymes agricultural residues |
description |
This work aimed to study the stationary and periodically mixed culture of L. edodes to the production of lignocellulolitic enzymes activity. LE 95/17, LE 96/22 and Leax strains were incubated in 25 g of eucalyptus sawdust substrate in Erlenmeyer flasks in stationary culture at 25 degrees C and in a bioreactor with four complete rotations daily at 25 degrees C and 3% CO2. The samples were collected at 8, 11, 14, 17 and 20 days after the incubation. Oxidative and hydrolytic enzymes analyses were performed. Lignin peroxidase enzyme was not found in the lignolytic systernfor LE 95/17, LE 96/22 and Leax strains in the different incubation methods. The use of bioreactor could be a practicable system to induce the laccase activity for L22 and Leax and MnP activity for L17 and L22. The activity of the hydrolytic enzymes was higher in the stationary system in comparison to periodically mixed system in the bioreactor. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-03-01 2014-05-20T13:53:33Z 2014-05-20T13:53:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1516-89132008000200001 Brazilian Archives of Biology and Technology. Curitiba-parana: Inst Tecnologia Parana, v. 51, n. 2, p. 223-233, 2008. 1516-8913 http://hdl.handle.net/11449/19113 S1516-89132008000200001 WOS:000256701300001 S1516-89132008000200001.pdf 3964089477817663 |
url |
http://dx.doi.org/10.1590/S1516-89132008000200001 http://hdl.handle.net/11449/19113 |
identifier_str_mv |
Brazilian Archives of Biology and Technology. Curitiba-parana: Inst Tecnologia Parana, v. 51, n. 2, p. 223-233, 2008. 1516-8913 S1516-89132008000200001 WOS:000256701300001 S1516-89132008000200001.pdf 3964089477817663 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Archives of Biology and Technology 0.676 0,281 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
223-233 application/pdf |
dc.publisher.none.fl_str_mv |
Inst Tecnologia Parana |
publisher.none.fl_str_mv |
Inst Tecnologia Parana |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803650370590736384 |