The Fagerström and AUDIT Tests as Probable Screening Tools in Oral Cancer and Their Correlation with CYP1A1, GSTM1, GSTP1, and GSTT1 Gene Expression
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ijerph19073991 http://hdl.handle.net/11449/230634 |
Resumo: | Background: Cancer is currently a major public health problem worldwide, with a marked increase of about 70% in the number of expected diagnosed cases over the next two decades. The amount of tobacco and alcohol consumed is calculated based on the subjective information provided by the user. Tobacco exposure can be assessed using the Fagerström Test for Cigarette Dependence (FTCD) and alcohol consumption by the Alcohol Use Disorder Identification Test (AUDIT). Materials and Methods: Forty-eight subjects answered the Fagerström, and AUDIT tests and we studied them as likely screening tools for oral cancer and their correlation with the expression of CYP1A1, GSTM1, GSTP1, and GSTT1 genes by the RT-qPCR method. Results: There were significant differences in the AUDIT score and CYP1A1 expression between cancer and control groups. Participants in advanced stages, whether due to tumor size or regional metastasis, showed significant differences in the duration of tobacco use, FTCD, AUDIT score, and CYP1A1 expression when compared to patients in early stages. Among subjects without cancer, we found a significant correlation between participant age and GSTP1 expression. Furthermore, the expression of GSTP1 was significantly correlated with the number of cigarettes smoked per day, duration of tobacco use, and FTCD. Conclusions: Questionnaires designed to evaluate the degree of tobacco and alcohol exposure and dependence combined with gene expression tests can be useful to assess the risk of developing oral cancer. Furthermore, raising the awareness of individuals regarding their degree of dependence and encouraging them to participate in cessation programs are important educational measures for the prevention of tobacco-related malignancies. |
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The Fagerström and AUDIT Tests as Probable Screening Tools in Oral Cancer and Their Correlation with CYP1A1, GSTM1, GSTP1, and GSTT1 Gene Expressionbuccal mucosacarcinogenesisgene expressionmouth neoplasmssquamous cell carcinomaxenobioticBackground: Cancer is currently a major public health problem worldwide, with a marked increase of about 70% in the number of expected diagnosed cases over the next two decades. The amount of tobacco and alcohol consumed is calculated based on the subjective information provided by the user. Tobacco exposure can be assessed using the Fagerström Test for Cigarette Dependence (FTCD) and alcohol consumption by the Alcohol Use Disorder Identification Test (AUDIT). Materials and Methods: Forty-eight subjects answered the Fagerström, and AUDIT tests and we studied them as likely screening tools for oral cancer and their correlation with the expression of CYP1A1, GSTM1, GSTP1, and GSTT1 genes by the RT-qPCR method. Results: There were significant differences in the AUDIT score and CYP1A1 expression between cancer and control groups. Participants in advanced stages, whether due to tumor size or regional metastasis, showed significant differences in the duration of tobacco use, FTCD, AUDIT score, and CYP1A1 expression when compared to patients in early stages. Among subjects without cancer, we found a significant correlation between participant age and GSTP1 expression. Furthermore, the expression of GSTP1 was significantly correlated with the number of cigarettes smoked per day, duration of tobacco use, and FTCD. Conclusions: Questionnaires designed to evaluate the degree of tobacco and alcohol exposure and dependence combined with gene expression tests can be useful to assess the risk of developing oral cancer. Furthermore, raising the awareness of individuals regarding their degree of dependence and encouraging them to participate in cessation programs are important educational measures for the prevention of tobacco-related malignancies.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Bioscience and Oral Diagnosis Institute of Science and Technology of São José dos Campos São Paulo State University (Unesp)Faculdade de Ciências Médicas de São José dos Campos—HumanitasSchool of Medicine Anhembi Morumbi UniversityTechnology Research Center (NPT) Universidade Mogi das CruzesDepartment of Head and Neck Surgery São Leopoldo Mandic CollegeDepartment of Head and Neck Surgery Hospital Municipal Doutor Mário GattiDepartment of Basic Sciences and Environment São Paulo UniversityDepartment of Oral Pathology School of Dentistry São Paulo UniversityDepartment of Bioscience and Oral Diagnosis Institute of Science and Technology of São José dos Campos São Paulo State University (Unesp)FAPESP: #2016/08633-0Universidade Estadual Paulista (UNESP)Faculdade de Ciências Médicas de São José dos Campos—HumanitasAnhembi Morumbi UniversityUniversidade Mogi das CruzesSão Leopoldo Mandic CollegeHospital Municipal Doutor Mário GattiUniversidade de São Paulo (USP)Bandeira, Celso Muller [UNESP]Almeida, Adriana Ávila [UNESP]Alves, Mônica Ghislaine OliveiraPascoal, Maria Beatriz NogueiraChagas, José Francisco SalesNeto, Morun Bernardinode Barros, Patrícia Pimentel [UNESP]Nunes, Fábio DaumasCarta, Celina Faig Lima [UNESP]Almeida, Janete Dias [UNESP]2022-04-29T08:41:18Z2022-04-29T08:41:18Z2022-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ijerph19073991International Journal of Environmental Research and Public Health, v. 19, n. 7, 2022.1660-46011661-7827http://hdl.handle.net/11449/23063410.3390/ijerph190739912-s2.0-85127046665Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Environmental Research and Public Healthinfo:eu-repo/semantics/openAccess2022-04-29T08:41:19Zoai:repositorio.unesp.br:11449/230634Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:54:41.111209Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The Fagerström and AUDIT Tests as Probable Screening Tools in Oral Cancer and Their Correlation with CYP1A1, GSTM1, GSTP1, and GSTT1 Gene Expression |
title |
The Fagerström and AUDIT Tests as Probable Screening Tools in Oral Cancer and Their Correlation with CYP1A1, GSTM1, GSTP1, and GSTT1 Gene Expression |
spellingShingle |
The Fagerström and AUDIT Tests as Probable Screening Tools in Oral Cancer and Their Correlation with CYP1A1, GSTM1, GSTP1, and GSTT1 Gene Expression Bandeira, Celso Muller [UNESP] buccal mucosa carcinogenesis gene expression mouth neoplasms squamous cell carcinoma xenobiotic |
title_short |
The Fagerström and AUDIT Tests as Probable Screening Tools in Oral Cancer and Their Correlation with CYP1A1, GSTM1, GSTP1, and GSTT1 Gene Expression |
title_full |
The Fagerström and AUDIT Tests as Probable Screening Tools in Oral Cancer and Their Correlation with CYP1A1, GSTM1, GSTP1, and GSTT1 Gene Expression |
title_fullStr |
The Fagerström and AUDIT Tests as Probable Screening Tools in Oral Cancer and Their Correlation with CYP1A1, GSTM1, GSTP1, and GSTT1 Gene Expression |
title_full_unstemmed |
The Fagerström and AUDIT Tests as Probable Screening Tools in Oral Cancer and Their Correlation with CYP1A1, GSTM1, GSTP1, and GSTT1 Gene Expression |
title_sort |
The Fagerström and AUDIT Tests as Probable Screening Tools in Oral Cancer and Their Correlation with CYP1A1, GSTM1, GSTP1, and GSTT1 Gene Expression |
author |
Bandeira, Celso Muller [UNESP] |
author_facet |
Bandeira, Celso Muller [UNESP] Almeida, Adriana Ávila [UNESP] Alves, Mônica Ghislaine Oliveira Pascoal, Maria Beatriz Nogueira Chagas, José Francisco Sales Neto, Morun Bernardino de Barros, Patrícia Pimentel [UNESP] Nunes, Fábio Daumas Carta, Celina Faig Lima [UNESP] Almeida, Janete Dias [UNESP] |
author_role |
author |
author2 |
Almeida, Adriana Ávila [UNESP] Alves, Mônica Ghislaine Oliveira Pascoal, Maria Beatriz Nogueira Chagas, José Francisco Sales Neto, Morun Bernardino de Barros, Patrícia Pimentel [UNESP] Nunes, Fábio Daumas Carta, Celina Faig Lima [UNESP] Almeida, Janete Dias [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Faculdade de Ciências Médicas de São José dos Campos—Humanitas Anhembi Morumbi University Universidade Mogi das Cruzes São Leopoldo Mandic College Hospital Municipal Doutor Mário Gatti Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Bandeira, Celso Muller [UNESP] Almeida, Adriana Ávila [UNESP] Alves, Mônica Ghislaine Oliveira Pascoal, Maria Beatriz Nogueira Chagas, José Francisco Sales Neto, Morun Bernardino de Barros, Patrícia Pimentel [UNESP] Nunes, Fábio Daumas Carta, Celina Faig Lima [UNESP] Almeida, Janete Dias [UNESP] |
dc.subject.por.fl_str_mv |
buccal mucosa carcinogenesis gene expression mouth neoplasms squamous cell carcinoma xenobiotic |
topic |
buccal mucosa carcinogenesis gene expression mouth neoplasms squamous cell carcinoma xenobiotic |
description |
Background: Cancer is currently a major public health problem worldwide, with a marked increase of about 70% in the number of expected diagnosed cases over the next two decades. The amount of tobacco and alcohol consumed is calculated based on the subjective information provided by the user. Tobacco exposure can be assessed using the Fagerström Test for Cigarette Dependence (FTCD) and alcohol consumption by the Alcohol Use Disorder Identification Test (AUDIT). Materials and Methods: Forty-eight subjects answered the Fagerström, and AUDIT tests and we studied them as likely screening tools for oral cancer and their correlation with the expression of CYP1A1, GSTM1, GSTP1, and GSTT1 genes by the RT-qPCR method. Results: There were significant differences in the AUDIT score and CYP1A1 expression between cancer and control groups. Participants in advanced stages, whether due to tumor size or regional metastasis, showed significant differences in the duration of tobacco use, FTCD, AUDIT score, and CYP1A1 expression when compared to patients in early stages. Among subjects without cancer, we found a significant correlation between participant age and GSTP1 expression. Furthermore, the expression of GSTP1 was significantly correlated with the number of cigarettes smoked per day, duration of tobacco use, and FTCD. Conclusions: Questionnaires designed to evaluate the degree of tobacco and alcohol exposure and dependence combined with gene expression tests can be useful to assess the risk of developing oral cancer. Furthermore, raising the awareness of individuals regarding their degree of dependence and encouraging them to participate in cessation programs are important educational measures for the prevention of tobacco-related malignancies. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-29T08:41:18Z 2022-04-29T08:41:18Z 2022-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ijerph19073991 International Journal of Environmental Research and Public Health, v. 19, n. 7, 2022. 1660-4601 1661-7827 http://hdl.handle.net/11449/230634 10.3390/ijerph19073991 2-s2.0-85127046665 |
url |
http://dx.doi.org/10.3390/ijerph19073991 http://hdl.handle.net/11449/230634 |
identifier_str_mv |
International Journal of Environmental Research and Public Health, v. 19, n. 7, 2022. 1660-4601 1661-7827 10.3390/ijerph19073991 2-s2.0-85127046665 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Environmental Research and Public Health |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128581798199296 |