Dietary carbohydrates and protein of yeast modulate the early stages of innate immune response in tilapia (Oreochromis niloticus) primarily after LPS inoculation
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10499-016-0073-2 http://hdl.handle.net/11449/178315 |
Resumo: | The administration of yeast-derived immunostimulants leads to improvement of the immune system and growth performance preventing the antibiotics misuses. The objective of this study was to evaluate the effects of dietary Actigen® [0, 0.04, 0.06 and 0.08 % of Actigen (ACT)], on the immunological and physiological responses and growth performance of tilapia after lipopolysaccharide (LPS) inoculation. The experiment was conducted in two trials. In the first trial, the ACT was offered for 30 and 60 days; in the second trial, the ACT was offered for 60 days, and then fish were challenged by intraperitoneal injection with LPS and sampled at 5 and 12 days after inoculation. After the first trial, immunological, hematological and biochemical parameters, intestinal morphology and the growth performance were assessed, whereas in the second trial, immunological, hematological and biochemical parameters were assessed. Supplementation of diets with ACT showed a significant effect mainly after 60 days of feeding, with increased lysozyme, globulin, HTC, MCV, erythroblasts, leukocytes, lymphocytes and monocytes. A general decrease in cells and globulin occurred at 5 days after the LPS challenge, with partial recovery at 12 days after the challenge. In addition, the study demonstrated that the use of ACT, an extract derived from yeast cell walls and containing active mannan protein and carbohydrates, effectively increased the immune parameters evaluated, indicating a possible beneficial effect on fish health. |
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Repositório Institucional da UNESP |
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Dietary carbohydrates and protein of yeast modulate the early stages of innate immune response in tilapia (Oreochromis niloticus) primarily after LPS inoculationActigenEndotoxinFishImmunostimulantPrebioticThe administration of yeast-derived immunostimulants leads to improvement of the immune system and growth performance preventing the antibiotics misuses. The objective of this study was to evaluate the effects of dietary Actigen® [0, 0.04, 0.06 and 0.08 % of Actigen (ACT)], on the immunological and physiological responses and growth performance of tilapia after lipopolysaccharide (LPS) inoculation. The experiment was conducted in two trials. In the first trial, the ACT was offered for 30 and 60 days; in the second trial, the ACT was offered for 60 days, and then fish were challenged by intraperitoneal injection with LPS and sampled at 5 and 12 days after inoculation. After the first trial, immunological, hematological and biochemical parameters, intestinal morphology and the growth performance were assessed, whereas in the second trial, immunological, hematological and biochemical parameters were assessed. Supplementation of diets with ACT showed a significant effect mainly after 60 days of feeding, with increased lysozyme, globulin, HTC, MCV, erythroblasts, leukocytes, lymphocytes and monocytes. A general decrease in cells and globulin occurred at 5 days after the LPS challenge, with partial recovery at 12 days after the challenge. In addition, the study demonstrated that the use of ACT, an extract derived from yeast cell walls and containing active mannan protein and carbohydrates, effectively increased the immune parameters evaluated, indicating a possible beneficial effect on fish health.AlltechFaculdade de Ciências Agrárias e Tecnológicas UNESP Univ Estadual Paulista, Rod. Cmte João Ribeiro de Barros, km 651Faculdade de Ciências Agrárias e Tecnológicas UNESP Univ Estadual Paulista, Rod. Cmte João Ribeiro de Barros, km 651Universidade Estadual Paulista (Unesp)Ha, Natalia [UNESP]Gonçalves, André Fernando Nascimento [UNESP]Sousa, Luana Camargo [UNESP]Biller-Takahashi, Jaqueline Dalbello [UNESP]Takahashi, Leonardo Susumu [UNESP]2018-12-11T17:29:45Z2018-12-11T17:29:45Z2017-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article755-776application/pdfhttp://dx.doi.org/10.1007/s10499-016-0073-2Aquaculture International, v. 25, n. 2, p. 755-776, 2017.1573-143X0967-6120http://hdl.handle.net/11449/17831510.1007/s10499-016-0073-22-s2.0-849898337092-s2.0-84989833709.pdf3860915077534516Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquaculture International0,591info:eu-repo/semantics/openAccess2024-05-07T13:47:22Zoai:repositorio.unesp.br:11449/178315Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:10:29.057638Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Dietary carbohydrates and protein of yeast modulate the early stages of innate immune response in tilapia (Oreochromis niloticus) primarily after LPS inoculation |
title |
Dietary carbohydrates and protein of yeast modulate the early stages of innate immune response in tilapia (Oreochromis niloticus) primarily after LPS inoculation |
spellingShingle |
Dietary carbohydrates and protein of yeast modulate the early stages of innate immune response in tilapia (Oreochromis niloticus) primarily after LPS inoculation Ha, Natalia [UNESP] Actigen Endotoxin Fish Immunostimulant Prebiotic |
title_short |
Dietary carbohydrates and protein of yeast modulate the early stages of innate immune response in tilapia (Oreochromis niloticus) primarily after LPS inoculation |
title_full |
Dietary carbohydrates and protein of yeast modulate the early stages of innate immune response in tilapia (Oreochromis niloticus) primarily after LPS inoculation |
title_fullStr |
Dietary carbohydrates and protein of yeast modulate the early stages of innate immune response in tilapia (Oreochromis niloticus) primarily after LPS inoculation |
title_full_unstemmed |
Dietary carbohydrates and protein of yeast modulate the early stages of innate immune response in tilapia (Oreochromis niloticus) primarily after LPS inoculation |
title_sort |
Dietary carbohydrates and protein of yeast modulate the early stages of innate immune response in tilapia (Oreochromis niloticus) primarily after LPS inoculation |
author |
Ha, Natalia [UNESP] |
author_facet |
Ha, Natalia [UNESP] Gonçalves, André Fernando Nascimento [UNESP] Sousa, Luana Camargo [UNESP] Biller-Takahashi, Jaqueline Dalbello [UNESP] Takahashi, Leonardo Susumu [UNESP] |
author_role |
author |
author2 |
Gonçalves, André Fernando Nascimento [UNESP] Sousa, Luana Camargo [UNESP] Biller-Takahashi, Jaqueline Dalbello [UNESP] Takahashi, Leonardo Susumu [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ha, Natalia [UNESP] Gonçalves, André Fernando Nascimento [UNESP] Sousa, Luana Camargo [UNESP] Biller-Takahashi, Jaqueline Dalbello [UNESP] Takahashi, Leonardo Susumu [UNESP] |
dc.subject.por.fl_str_mv |
Actigen Endotoxin Fish Immunostimulant Prebiotic |
topic |
Actigen Endotoxin Fish Immunostimulant Prebiotic |
description |
The administration of yeast-derived immunostimulants leads to improvement of the immune system and growth performance preventing the antibiotics misuses. The objective of this study was to evaluate the effects of dietary Actigen® [0, 0.04, 0.06 and 0.08 % of Actigen (ACT)], on the immunological and physiological responses and growth performance of tilapia after lipopolysaccharide (LPS) inoculation. The experiment was conducted in two trials. In the first trial, the ACT was offered for 30 and 60 days; in the second trial, the ACT was offered for 60 days, and then fish were challenged by intraperitoneal injection with LPS and sampled at 5 and 12 days after inoculation. After the first trial, immunological, hematological and biochemical parameters, intestinal morphology and the growth performance were assessed, whereas in the second trial, immunological, hematological and biochemical parameters were assessed. Supplementation of diets with ACT showed a significant effect mainly after 60 days of feeding, with increased lysozyme, globulin, HTC, MCV, erythroblasts, leukocytes, lymphocytes and monocytes. A general decrease in cells and globulin occurred at 5 days after the LPS challenge, with partial recovery at 12 days after the challenge. In addition, the study demonstrated that the use of ACT, an extract derived from yeast cell walls and containing active mannan protein and carbohydrates, effectively increased the immune parameters evaluated, indicating a possible beneficial effect on fish health. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-04-01 2018-12-11T17:29:45Z 2018-12-11T17:29:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10499-016-0073-2 Aquaculture International, v. 25, n. 2, p. 755-776, 2017. 1573-143X 0967-6120 http://hdl.handle.net/11449/178315 10.1007/s10499-016-0073-2 2-s2.0-84989833709 2-s2.0-84989833709.pdf 3860915077534516 |
url |
http://dx.doi.org/10.1007/s10499-016-0073-2 http://hdl.handle.net/11449/178315 |
identifier_str_mv |
Aquaculture International, v. 25, n. 2, p. 755-776, 2017. 1573-143X 0967-6120 10.1007/s10499-016-0073-2 2-s2.0-84989833709 2-s2.0-84989833709.pdf 3860915077534516 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aquaculture International 0,591 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
755-776 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128766875009024 |