Cytotoxicity of acrylic resin-based materials used to fabricate interim crowns
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.prosdent.2020.01.030 http://hdl.handle.net/11449/201647 |
Resumo: | Statement of problem: If the components in the acrylic resins used to fabricate interim crows are cytotoxic, they can interfere with the integrity of the adjacent periodontal tissue and the dentin-pulp complex. Purpose: The purpose of this in vitro study was to assess the cytotoxicity of resin-based materials used to prepare interim crowns. Material and methods: The following materials were used in this study: CAR, conventional acrylic resin powder and liquid; BR, bis-acrylic resin; and PAR, pressed acrylic resin of the CAD-CAM type. Glass disks were used as the control (Co). Oral epithelial cells (NOK) were seeded on glass disks and standardized disks prepared with the resins under study. After incubation for 24 hours, the cells were analyzed for viability (Alamar Blue and Live or Dead), adhesion, and morphology (SEM and fluorescence), as well as epidermal growth factor synthesis (EGF-ELISA). The surface roughness (Ra) of test specimens was evaluated under a confocal microscope. The data were submitted to ANOVA and the Tukey HSD statistical tests (α=.05). Results: The highest Ra value was observed in BR in comparison with CAR, PAR, and Co (P<.05). The highest viability, adhesion, and EGF synthesis values were determined for the cells in contact with PAR (P<.001). Conclusions: The computer-aided design and computer-aided manufacturing (CAD-CAM)-type resin favored adhesion, metabolism, and epithelial cell proliferation, and it was therefore considered cytocompatible. |
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Cytotoxicity of acrylic resin-based materials used to fabricate interim crownsStatement of problem: If the components in the acrylic resins used to fabricate interim crows are cytotoxic, they can interfere with the integrity of the adjacent periodontal tissue and the dentin-pulp complex. Purpose: The purpose of this in vitro study was to assess the cytotoxicity of resin-based materials used to prepare interim crowns. Material and methods: The following materials were used in this study: CAR, conventional acrylic resin powder and liquid; BR, bis-acrylic resin; and PAR, pressed acrylic resin of the CAD-CAM type. Glass disks were used as the control (Co). Oral epithelial cells (NOK) were seeded on glass disks and standardized disks prepared with the resins under study. After incubation for 24 hours, the cells were analyzed for viability (Alamar Blue and Live or Dead), adhesion, and morphology (SEM and fluorescence), as well as epidermal growth factor synthesis (EGF-ELISA). The surface roughness (Ra) of test specimens was evaluated under a confocal microscope. The data were submitted to ANOVA and the Tukey HSD statistical tests (α=.05). Results: The highest Ra value was observed in BR in comparison with CAR, PAR, and Co (P<.05). The highest viability, adhesion, and EGF synthesis values were determined for the cells in contact with PAR (P<.001). Conclusions: The computer-aided design and computer-aided manufacturing (CAD-CAM)-type resin favored adhesion, metabolism, and epithelial cell proliferation, and it was therefore considered cytocompatible.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)ASCRS Research FoundationFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Masters student Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)Doctoral student Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)Department of Dentistry The University of Ribeirão Preto (UNAERP)Department of Orthodontics and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP)Department of Physiology and Pathology Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)Masters student Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)Doctoral student Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)Department of Orthodontics and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP)Department of Physiology and Pathology Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)FAPESP: 2017/10710-6CNPq: 302047/2019-0Universidade Estadual Paulista (Unesp)The University of Ribeirão Preto (UNAERP)Souza, Isabela R. [UNESP]Pansani, Taisa N. [UNESP]Basso, Fernanda G.Hebling, Josimeri [UNESP]de Souza Costa, Carlos A. [UNESP]2020-12-12T02:38:05Z2020-12-12T02:38:05Z2020-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject122.e1-122.e9http://dx.doi.org/10.1016/j.prosdent.2020.01.030Journal of Prosthetic Dentistry, v. 124, n. 1, p. 122.e1-122.e9, 2020.1097-68410022-3913http://hdl.handle.net/11449/20164710.1016/j.prosdent.2020.01.0302-s2.0-85082191821Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Prosthetic Dentistryinfo:eu-repo/semantics/openAccess2021-10-22T20:43:00Zoai:repositorio.unesp.br:11449/201647Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:52:40.616491Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytotoxicity of acrylic resin-based materials used to fabricate interim crowns |
title |
Cytotoxicity of acrylic resin-based materials used to fabricate interim crowns |
spellingShingle |
Cytotoxicity of acrylic resin-based materials used to fabricate interim crowns Souza, Isabela R. [UNESP] |
title_short |
Cytotoxicity of acrylic resin-based materials used to fabricate interim crowns |
title_full |
Cytotoxicity of acrylic resin-based materials used to fabricate interim crowns |
title_fullStr |
Cytotoxicity of acrylic resin-based materials used to fabricate interim crowns |
title_full_unstemmed |
Cytotoxicity of acrylic resin-based materials used to fabricate interim crowns |
title_sort |
Cytotoxicity of acrylic resin-based materials used to fabricate interim crowns |
author |
Souza, Isabela R. [UNESP] |
author_facet |
Souza, Isabela R. [UNESP] Pansani, Taisa N. [UNESP] Basso, Fernanda G. Hebling, Josimeri [UNESP] de Souza Costa, Carlos A. [UNESP] |
author_role |
author |
author2 |
Pansani, Taisa N. [UNESP] Basso, Fernanda G. Hebling, Josimeri [UNESP] de Souza Costa, Carlos A. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) The University of Ribeirão Preto (UNAERP) |
dc.contributor.author.fl_str_mv |
Souza, Isabela R. [UNESP] Pansani, Taisa N. [UNESP] Basso, Fernanda G. Hebling, Josimeri [UNESP] de Souza Costa, Carlos A. [UNESP] |
description |
Statement of problem: If the components in the acrylic resins used to fabricate interim crows are cytotoxic, they can interfere with the integrity of the adjacent periodontal tissue and the dentin-pulp complex. Purpose: The purpose of this in vitro study was to assess the cytotoxicity of resin-based materials used to prepare interim crowns. Material and methods: The following materials were used in this study: CAR, conventional acrylic resin powder and liquid; BR, bis-acrylic resin; and PAR, pressed acrylic resin of the CAD-CAM type. Glass disks were used as the control (Co). Oral epithelial cells (NOK) were seeded on glass disks and standardized disks prepared with the resins under study. After incubation for 24 hours, the cells were analyzed for viability (Alamar Blue and Live or Dead), adhesion, and morphology (SEM and fluorescence), as well as epidermal growth factor synthesis (EGF-ELISA). The surface roughness (Ra) of test specimens was evaluated under a confocal microscope. The data were submitted to ANOVA and the Tukey HSD statistical tests (α=.05). Results: The highest Ra value was observed in BR in comparison with CAR, PAR, and Co (P<.05). The highest viability, adhesion, and EGF synthesis values were determined for the cells in contact with PAR (P<.001). Conclusions: The computer-aided design and computer-aided manufacturing (CAD-CAM)-type resin favored adhesion, metabolism, and epithelial cell proliferation, and it was therefore considered cytocompatible. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:38:05Z 2020-12-12T02:38:05Z 2020-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.prosdent.2020.01.030 Journal of Prosthetic Dentistry, v. 124, n. 1, p. 122.e1-122.e9, 2020. 1097-6841 0022-3913 http://hdl.handle.net/11449/201647 10.1016/j.prosdent.2020.01.030 2-s2.0-85082191821 |
url |
http://dx.doi.org/10.1016/j.prosdent.2020.01.030 http://hdl.handle.net/11449/201647 |
identifier_str_mv |
Journal of Prosthetic Dentistry, v. 124, n. 1, p. 122.e1-122.e9, 2020. 1097-6841 0022-3913 10.1016/j.prosdent.2020.01.030 2-s2.0-85082191821 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Prosthetic Dentistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
122.e1-122.e9 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128869627068416 |