PTEN expression in patients with carcinoma of the cervix and its association with p53, Ki-67 and CD31

Detalhes bibliográficos
Autor(a) principal: Loures, Luciano Fernandes
Data de Publicação: 2014
Outros Autores: Cândido, Eduardo Batista, Vidigal, Paula Vieira Teixeira, Seabra, Mariana Ataydes Leite, Marco, Luiz Armando Cunha de, Silva Filho, Agnaldo Lopes da [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0100-7203201400050004
http://hdl.handle.net/11449/140860
Resumo: PURPOSE: To investigate protein expression and mutations in phosphatase and tensin homolog (PTEN) in patients with stage IB cervical squamous cell carcinoma (CSCC) and the association with clinical-pathologic features, tumor p53 expression, cell proliferation and angiogenesis. METHODS: Women with stage IB CSCC (n=20 – Study Group) and uterine myoma (n=20 – Control Group), aged 49.1±1.7 years (mean±standard deviation, range 27–78 years), were prospectively evaluated. Patients with cervical cancer were submitted to Piver-Rutledge class III radical hysterectomy and pelvic lymphadenectomy and patients in the Control Group underwent vaginal hysterectomy. Tissue samples from the procedures were stained with hematoxylin and eosin for histological evaluation. Protein expression was detected by immunohistochemistry. Staining for PTEN, p53, Ki-67 and CD31 was evaluated. The intensity of PTEN immunostaining was estimated by computer-assisted image analysis, based on previously reported protocols. Data were analyzed using the Student’s t-test to evaluate significant differences between the groups. Level of significance was set at p<0.05. RESULTS: The PTEN expression intensity was lower in the CSCC group than in the Control (benign cervix) samples (150.5±5.2 versus 204.2±2.6; p<0.001). Our study did not identify any mutations after sequencing all nine PTEN exons. PTEN expression was not associated with tumor expression of p53 (p=0.9), CD31 (p=0.8) or Ki-67 (p=0.3) or clinical-pathologic features in patients with invasive carcinoma of the cervix. CONCLUSIONS: Our findings demonstrate that the PTEN protein expression is significantly diminished in CSCC.
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spelling PTEN expression in patients with carcinoma of the cervix and its association with p53, Ki-67 and CD31Expressão do PTEN em pacientes com carcinoma de colo uterino e sua associação com p53, Ki-67 e CD31Uterine cervical neoplasmsImmunohistochemistryGene expressionAntigens, neoplasmNeoplasias do colo do úteroImunohistoquímicaExpressão gênicaAntígenos de neoplasiasPURPOSE: To investigate protein expression and mutations in phosphatase and tensin homolog (PTEN) in patients with stage IB cervical squamous cell carcinoma (CSCC) and the association with clinical-pathologic features, tumor p53 expression, cell proliferation and angiogenesis. METHODS: Women with stage IB CSCC (n=20 – Study Group) and uterine myoma (n=20 – Control Group), aged 49.1±1.7 years (mean±standard deviation, range 27–78 years), were prospectively evaluated. Patients with cervical cancer were submitted to Piver-Rutledge class III radical hysterectomy and pelvic lymphadenectomy and patients in the Control Group underwent vaginal hysterectomy. Tissue samples from the procedures were stained with hematoxylin and eosin for histological evaluation. Protein expression was detected by immunohistochemistry. Staining for PTEN, p53, Ki-67 and CD31 was evaluated. The intensity of PTEN immunostaining was estimated by computer-assisted image analysis, based on previously reported protocols. Data were analyzed using the Student’s t-test to evaluate significant differences between the groups. Level of significance was set at p<0.05. RESULTS: The PTEN expression intensity was lower in the CSCC group than in the Control (benign cervix) samples (150.5±5.2 versus 204.2±2.6; p<0.001). Our study did not identify any mutations after sequencing all nine PTEN exons. PTEN expression was not associated with tumor expression of p53 (p=0.9), CD31 (p=0.8) or Ki-67 (p=0.3) or clinical-pathologic features in patients with invasive carcinoma of the cervix. CONCLUSIONS: Our findings demonstrate that the PTEN protein expression is significantly diminished in CSCC.OBJETIVO: O objetivo do estudo foi investigar a expressão e mutações do PTEN em pacientes com Carcinoma de Células Escamosas (CCE) de Colo do Útero com estadiamento IB e sua associação com fatores prognósticos, expressão do p53, proliferação celular e angiogênese. MÉTODOS: Mulheres com diagnóstico de CCE de colo uterino em estágio IB (n=20) (casos) e mioma uterino (n=20) (controle) com idade de 49.1±1.7 foram acompanhadas. As pacientes com câncer de colo do útero foram submetidas a histerectomia Piver-Rutledge classe III associada a linfadenectomia pélvica e aquelas com mioma uterino a histerectomia vaginal. Amostras de tumor e colo normal foram retiradas para avaliação histológica e marcação imuno-histoquímica das proteínas PTEN, p53, ki-67 e CD 3. A intensidade imunohistoquímica do PTEN foi estimada por processamento de imagem digital a partir de protocolos pré-estabelecidos. Os dados foram analisados através do teste de qui - quadrado (χ2). O nível de significância foi considerado quando p < 0,05. RESULTADOS: A expressão do PTEN estava diminuída no grupo de pacientes com CCE em comparação ao grupo controle (150.5±5.2 versus 204.2±2.6; p<0.001). Nenhuma mutação no seqüenciamento genético dos nove exons do PTEN foi encontrada. Não houve associação estatisticamente significativa entre a expressão do PTEN e a expressão do p53 (p=0,969), Ki-67 (p=0.283) e CD 31 (p=0.817) ou fatores prognósticos anátomo-clínicos nas pacientes com carcinoma invasor do colo uterino. CONCLUSÕES: Este estudo demonstrou que o PTEN estava significativamente diminuído nas pacientes com CCE.Universidade Federal de Juiz de Fora (UFJF), Juiz de Fora, MG, BrasilUniversidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, BrasilUniversidade Estadual Paulista Julio de Mesquita Filho (UNESP), Faculdade de Medicina de Botucatu (FMB), Departamento de Ginecologia e Obstetrícia, Botucatu, SP, BrasilUniversidade Estadual Paulista Julio de Mesquita Filho (UNESP), Faculdade de Medicina de Botucatu (FMB), Departamento de Ginecologia e Obstetrícia, Botucatu, SP, BrasilUniversidade Federal de Juiz de Fora (UFJF)Universidade Federal de Minas Gerais (UFMG)Universidade Estadual Paulista (Unesp)Loures, Luciano FernandesCândido, Eduardo BatistaVidigal, Paula Vieira TeixeiraSeabra, Mariana Ataydes LeiteMarco, Luiz Armando Cunha deSilva Filho, Agnaldo Lopes da [UNESP]2016-07-07T12:35:45Z2016-07-07T12:35:45Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article205-210application/pdfhttp://dx.doi.org/10.1590/S0100-7203201400050004Revista Brasileira de Ginecologia e Obstetrícia, v. 36, n. 5, p. 205-210, 2014.0100-7203http://hdl.handle.net/11449/14086010.1590/S0100-7203201400050004S0100-72032014000500205S0100-72032014000500205.pdfCurrículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Ginecologia e Obstetrícia0,292info:eu-repo/semantics/openAccess2024-08-16T14:13:01Zoai:repositorio.unesp.br:11449/140860Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:13:01Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv PTEN expression in patients with carcinoma of the cervix and its association with p53, Ki-67 and CD31
Expressão do PTEN em pacientes com carcinoma de colo uterino e sua associação com p53, Ki-67 e CD31
title PTEN expression in patients with carcinoma of the cervix and its association with p53, Ki-67 and CD31
spellingShingle PTEN expression in patients with carcinoma of the cervix and its association with p53, Ki-67 and CD31
Loures, Luciano Fernandes
Uterine cervical neoplasms
Immunohistochemistry
Gene expression
Antigens, neoplasm
Neoplasias do colo do útero
Imunohistoquímica
Expressão gênica
Antígenos de neoplasias
title_short PTEN expression in patients with carcinoma of the cervix and its association with p53, Ki-67 and CD31
title_full PTEN expression in patients with carcinoma of the cervix and its association with p53, Ki-67 and CD31
title_fullStr PTEN expression in patients with carcinoma of the cervix and its association with p53, Ki-67 and CD31
title_full_unstemmed PTEN expression in patients with carcinoma of the cervix and its association with p53, Ki-67 and CD31
title_sort PTEN expression in patients with carcinoma of the cervix and its association with p53, Ki-67 and CD31
author Loures, Luciano Fernandes
author_facet Loures, Luciano Fernandes
Cândido, Eduardo Batista
Vidigal, Paula Vieira Teixeira
Seabra, Mariana Ataydes Leite
Marco, Luiz Armando Cunha de
Silva Filho, Agnaldo Lopes da [UNESP]
author_role author
author2 Cândido, Eduardo Batista
Vidigal, Paula Vieira Teixeira
Seabra, Mariana Ataydes Leite
Marco, Luiz Armando Cunha de
Silva Filho, Agnaldo Lopes da [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Juiz de Fora (UFJF)
Universidade Federal de Minas Gerais (UFMG)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Loures, Luciano Fernandes
Cândido, Eduardo Batista
Vidigal, Paula Vieira Teixeira
Seabra, Mariana Ataydes Leite
Marco, Luiz Armando Cunha de
Silva Filho, Agnaldo Lopes da [UNESP]
dc.subject.por.fl_str_mv Uterine cervical neoplasms
Immunohistochemistry
Gene expression
Antigens, neoplasm
Neoplasias do colo do útero
Imunohistoquímica
Expressão gênica
Antígenos de neoplasias
topic Uterine cervical neoplasms
Immunohistochemistry
Gene expression
Antigens, neoplasm
Neoplasias do colo do útero
Imunohistoquímica
Expressão gênica
Antígenos de neoplasias
description PURPOSE: To investigate protein expression and mutations in phosphatase and tensin homolog (PTEN) in patients with stage IB cervical squamous cell carcinoma (CSCC) and the association with clinical-pathologic features, tumor p53 expression, cell proliferation and angiogenesis. METHODS: Women with stage IB CSCC (n=20 – Study Group) and uterine myoma (n=20 – Control Group), aged 49.1±1.7 years (mean±standard deviation, range 27–78 years), were prospectively evaluated. Patients with cervical cancer were submitted to Piver-Rutledge class III radical hysterectomy and pelvic lymphadenectomy and patients in the Control Group underwent vaginal hysterectomy. Tissue samples from the procedures were stained with hematoxylin and eosin for histological evaluation. Protein expression was detected by immunohistochemistry. Staining for PTEN, p53, Ki-67 and CD31 was evaluated. The intensity of PTEN immunostaining was estimated by computer-assisted image analysis, based on previously reported protocols. Data were analyzed using the Student’s t-test to evaluate significant differences between the groups. Level of significance was set at p<0.05. RESULTS: The PTEN expression intensity was lower in the CSCC group than in the Control (benign cervix) samples (150.5±5.2 versus 204.2±2.6; p<0.001). Our study did not identify any mutations after sequencing all nine PTEN exons. PTEN expression was not associated with tumor expression of p53 (p=0.9), CD31 (p=0.8) or Ki-67 (p=0.3) or clinical-pathologic features in patients with invasive carcinoma of the cervix. CONCLUSIONS: Our findings demonstrate that the PTEN protein expression is significantly diminished in CSCC.
publishDate 2014
dc.date.none.fl_str_mv 2014
2016-07-07T12:35:45Z
2016-07-07T12:35:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0100-7203201400050004
Revista Brasileira de Ginecologia e Obstetrícia, v. 36, n. 5, p. 205-210, 2014.
0100-7203
http://hdl.handle.net/11449/140860
10.1590/S0100-7203201400050004
S0100-72032014000500205
S0100-72032014000500205.pdf
url http://dx.doi.org/10.1590/S0100-7203201400050004
http://hdl.handle.net/11449/140860
identifier_str_mv Revista Brasileira de Ginecologia e Obstetrícia, v. 36, n. 5, p. 205-210, 2014.
0100-7203
10.1590/S0100-7203201400050004
S0100-72032014000500205
S0100-72032014000500205.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Ginecologia e Obstetrícia
0,292
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 205-210
application/pdf
dc.source.none.fl_str_mv Currículo Lattes
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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