Levels of cardiorespiratory fitness in men exerts strong impact on lymphocyte function after mitogen stimulation
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1152/japplphysiol.01051.2020 http://hdl.handle.net/11449/206205 |
Resumo: | Relationship between lymphocyte function and cardiorespiratory fitness (CRF) is well-documented at rest; however, upon mitogen stimulation the proliferation and cytokine production alters, but knowledge is incipient about lymphocyte responses after mitogen stimulus according to CRF. So, the purpose of the present study was to analyze the lymphocyte function according to the physical fitness status of healthy young men. The study is divided in two experiments being the first analyzing the lymphocyte phenotypes profile and the inflammatory responses, according to CRF, in lymphocyte cell cultures treated for 48 h with concanavalin A (ConA). The second experiment analyzed the proliferation, reactive oxygen species production, viability, and mitochondrial polarization state in lymphocytes treated with ConA in different concentrations, considering the CRF levels. The results showed a difference in the percentage of total lymphocytes expression between groups (P = 0.011) observing a lower lymphocytes T expression in the group with high maximal oxygen consumption (V_ O2max) when compared with the moderate V_ O2max group. When treated with ConA, the lymphocytes of the low V_ O2max group released higher TNF-a concentration (P = 0.032), reflecting an elevated TNF-a/IL-10 ratio (P = 0.055), parallel with lower IL-6 production (P = 0.027), mainly when compared with the moderate V_ O2max group. In addition, there is a positive relationship between V_ O2max and IL-6 production (r = 0.507; P = 0.016), whereas the percentage of total lymphocytes (LyT%) shows a negative trend with V_ O2max (r = 0.497; P = 0.060). Also, individuals with lower V_ O2max showed reduced absolute and relative ROS production, lower cell proliferation, and higher mitochondrial membrane depolarization. In conclusion, cardiorespiratory fitness degree exerts a strong impact on lymphocyte function after mitogen stimulation. NEW & NOTEWORTHY The innovation of the research is to elucidate the impact of different physical fitness status on metabolism, cell proliferation, and lymphocyte activity and, consequently, on the specific inflammatory response against a mitogen. |
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Levels of cardiorespiratory fitness in men exerts strong impact on lymphocyte function after mitogen stimulationImmune systemInflammationOxidative stressPhysical fitness statusRelationship between lymphocyte function and cardiorespiratory fitness (CRF) is well-documented at rest; however, upon mitogen stimulation the proliferation and cytokine production alters, but knowledge is incipient about lymphocyte responses after mitogen stimulus according to CRF. So, the purpose of the present study was to analyze the lymphocyte function according to the physical fitness status of healthy young men. The study is divided in two experiments being the first analyzing the lymphocyte phenotypes profile and the inflammatory responses, according to CRF, in lymphocyte cell cultures treated for 48 h with concanavalin A (ConA). The second experiment analyzed the proliferation, reactive oxygen species production, viability, and mitochondrial polarization state in lymphocytes treated with ConA in different concentrations, considering the CRF levels. The results showed a difference in the percentage of total lymphocytes expression between groups (P = 0.011) observing a lower lymphocytes T expression in the group with high maximal oxygen consumption (V_ O2max) when compared with the moderate V_ O2max group. When treated with ConA, the lymphocytes of the low V_ O2max group released higher TNF-a concentration (P = 0.032), reflecting an elevated TNF-a/IL-10 ratio (P = 0.055), parallel with lower IL-6 production (P = 0.027), mainly when compared with the moderate V_ O2max group. In addition, there is a positive relationship between V_ O2max and IL-6 production (r = 0.507; P = 0.016), whereas the percentage of total lymphocytes (LyT%) shows a negative trend with V_ O2max (r = 0.497; P = 0.060). Also, individuals with lower V_ O2max showed reduced absolute and relative ROS production, lower cell proliferation, and higher mitochondrial membrane depolarization. In conclusion, cardiorespiratory fitness degree exerts a strong impact on lymphocyte function after mitogen stimulation. NEW & NOTEWORTHY The innovation of the research is to elucidate the impact of different physical fitness status on metabolism, cell proliferation, and lymphocyte activity and, consequently, on the specific inflammatory response against a mitogen.Cellular and Molecular Immunology Lab Universidade Federal de Ciências da Saúde de Porto AlegreExercise and Immunometabolism Research Group Postgraduation Program in Movement Sciences Department of Physical Education Universidade Estadual Paulista (UNESP)Immunometabolism Research Group Institute of Biomedical Sciences University of São Paulo (USP)Department of Exercise Physiology and Sports Therapy Justus-Liebig-University GiessenExercise and Immunometabolism Research Group Postgraduation Program in Movement Sciences Department of Physical Education Universidade Estadual Paulista (UNESP)Universidade Federal de Ciências da Saúde de Porto AlegreUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Justus-Liebig-University GiessenDorneles, Gilson P.Lira, Fabio Santos [UNESP]Romão, Pedro R.T.Kruger, KarstenRosa-Neto, Jose CesarPeres, AlessandraAntunes, Barbara Moura [UNESP]2021-06-25T10:28:13Z2021-06-25T10:28:13Z2021-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1133-1142http://dx.doi.org/10.1152/japplphysiol.01051.2020Journal of Applied Physiology, v. 130, n. 4, p. 1133-1142, 2021.1522-16018750-7587http://hdl.handle.net/11449/20620510.1152/japplphysiol.01051.20202-s2.0-85104321096Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Physiologyinfo:eu-repo/semantics/openAccess2021-10-22T22:23:36Zoai:repositorio.unesp.br:11449/206205Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T22:23:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Levels of cardiorespiratory fitness in men exerts strong impact on lymphocyte function after mitogen stimulation |
title |
Levels of cardiorespiratory fitness in men exerts strong impact on lymphocyte function after mitogen stimulation |
spellingShingle |
Levels of cardiorespiratory fitness in men exerts strong impact on lymphocyte function after mitogen stimulation Dorneles, Gilson P. Immune system Inflammation Oxidative stress Physical fitness status |
title_short |
Levels of cardiorespiratory fitness in men exerts strong impact on lymphocyte function after mitogen stimulation |
title_full |
Levels of cardiorespiratory fitness in men exerts strong impact on lymphocyte function after mitogen stimulation |
title_fullStr |
Levels of cardiorespiratory fitness in men exerts strong impact on lymphocyte function after mitogen stimulation |
title_full_unstemmed |
Levels of cardiorespiratory fitness in men exerts strong impact on lymphocyte function after mitogen stimulation |
title_sort |
Levels of cardiorespiratory fitness in men exerts strong impact on lymphocyte function after mitogen stimulation |
author |
Dorneles, Gilson P. |
author_facet |
Dorneles, Gilson P. Lira, Fabio Santos [UNESP] Romão, Pedro R.T. Kruger, Karsten Rosa-Neto, Jose Cesar Peres, Alessandra Antunes, Barbara Moura [UNESP] |
author_role |
author |
author2 |
Lira, Fabio Santos [UNESP] Romão, Pedro R.T. Kruger, Karsten Rosa-Neto, Jose Cesar Peres, Alessandra Antunes, Barbara Moura [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Ciências da Saúde de Porto Alegre Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Justus-Liebig-University Giessen |
dc.contributor.author.fl_str_mv |
Dorneles, Gilson P. Lira, Fabio Santos [UNESP] Romão, Pedro R.T. Kruger, Karsten Rosa-Neto, Jose Cesar Peres, Alessandra Antunes, Barbara Moura [UNESP] |
dc.subject.por.fl_str_mv |
Immune system Inflammation Oxidative stress Physical fitness status |
topic |
Immune system Inflammation Oxidative stress Physical fitness status |
description |
Relationship between lymphocyte function and cardiorespiratory fitness (CRF) is well-documented at rest; however, upon mitogen stimulation the proliferation and cytokine production alters, but knowledge is incipient about lymphocyte responses after mitogen stimulus according to CRF. So, the purpose of the present study was to analyze the lymphocyte function according to the physical fitness status of healthy young men. The study is divided in two experiments being the first analyzing the lymphocyte phenotypes profile and the inflammatory responses, according to CRF, in lymphocyte cell cultures treated for 48 h with concanavalin A (ConA). The second experiment analyzed the proliferation, reactive oxygen species production, viability, and mitochondrial polarization state in lymphocytes treated with ConA in different concentrations, considering the CRF levels. The results showed a difference in the percentage of total lymphocytes expression between groups (P = 0.011) observing a lower lymphocytes T expression in the group with high maximal oxygen consumption (V_ O2max) when compared with the moderate V_ O2max group. When treated with ConA, the lymphocytes of the low V_ O2max group released higher TNF-a concentration (P = 0.032), reflecting an elevated TNF-a/IL-10 ratio (P = 0.055), parallel with lower IL-6 production (P = 0.027), mainly when compared with the moderate V_ O2max group. In addition, there is a positive relationship between V_ O2max and IL-6 production (r = 0.507; P = 0.016), whereas the percentage of total lymphocytes (LyT%) shows a negative trend with V_ O2max (r = 0.497; P = 0.060). Also, individuals with lower V_ O2max showed reduced absolute and relative ROS production, lower cell proliferation, and higher mitochondrial membrane depolarization. In conclusion, cardiorespiratory fitness degree exerts a strong impact on lymphocyte function after mitogen stimulation. NEW & NOTEWORTHY The innovation of the research is to elucidate the impact of different physical fitness status on metabolism, cell proliferation, and lymphocyte activity and, consequently, on the specific inflammatory response against a mitogen. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:28:13Z 2021-06-25T10:28:13Z 2021-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1152/japplphysiol.01051.2020 Journal of Applied Physiology, v. 130, n. 4, p. 1133-1142, 2021. 1522-1601 8750-7587 http://hdl.handle.net/11449/206205 10.1152/japplphysiol.01051.2020 2-s2.0-85104321096 |
url |
http://dx.doi.org/10.1152/japplphysiol.01051.2020 http://hdl.handle.net/11449/206205 |
identifier_str_mv |
Journal of Applied Physiology, v. 130, n. 4, p. 1133-1142, 2021. 1522-1601 8750-7587 10.1152/japplphysiol.01051.2020 2-s2.0-85104321096 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Physiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1133-1142 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964767049220096 |