Influência da raça do touro (Bos indicus x Bos taurus) na tolerância ao estresse térmico calórico de embriões bovinos produzidos in vitro
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.revistas.usp.br/bjvras/article/view/34399 http://hdl.handle.net/11449/73012 |
Resumo: | To better understand the differences related to HS resistance between Bos indicus and Bos taurus, we aim to verify if the HS tolerance is due mostly to the genetic contribution from the oocyte, spermatozoa or both. Oocytes from Nelore and crossbreed Holstein cows (cHST) were collected, matured and fertilized with semen from Nelore (N), Angus (An), Brahman (Bra) and Gir (Gir) bulls. Nine six hours post insemination (hpi), ≥ 16 cells embryos were separated in two groups: control and HS. In control group, embryos were cultured at 39°C, whereas in the HS group, embryos were subjected to 41°C for 12 h, and then returned to 39°C. There was no effect of HS on blastocyst and hatched blastocyst rates in all breeds analyzed. The percentage of oocytes that cleaved and reached morula stage was significantly lower (p < 0.05) in cHST x Gir as compared to the other breeds. Additionally, blastocyst rates was higher in cHST x N than in cHST x An and cHST x Gir (p < 0.05). It was concluded that the oocyte is more important than the spermatozoa for the development of thermotolerance, since the breed of the bull did not influence embryo development after HS. |
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Influência da raça do touro (Bos indicus x Bos taurus) na tolerância ao estresse térmico calórico de embriões bovinos produzidos in vitroInfluence of sire breed (Bos indicus vs Bos taurus) on heat stress tolerance of in vitroproduced bovine embryosBlastocystBovineHeat stressIn vitro productionBos indicusBos taurusBovinaeTo better understand the differences related to HS resistance between Bos indicus and Bos taurus, we aim to verify if the HS tolerance is due mostly to the genetic contribution from the oocyte, spermatozoa or both. Oocytes from Nelore and crossbreed Holstein cows (cHST) were collected, matured and fertilized with semen from Nelore (N), Angus (An), Brahman (Bra) and Gir (Gir) bulls. Nine six hours post insemination (hpi), ≥ 16 cells embryos were separated in two groups: control and HS. In control group, embryos were cultured at 39°C, whereas in the HS group, embryos were subjected to 41°C for 12 h, and then returned to 39°C. There was no effect of HS on blastocyst and hatched blastocyst rates in all breeds analyzed. The percentage of oocytes that cleaved and reached morula stage was significantly lower (p < 0.05) in cHST x Gir as compared to the other breeds. Additionally, blastocyst rates was higher in cHST x N than in cHST x An and cHST x Gir (p < 0.05). It was concluded that the oocyte is more important than the spermatozoa for the development of thermotolerance, since the breed of the bull did not influence embryo development after HS.Departamento de Farmacologia do Instituto de Biociências da Universidade Estadual Paulista, Botucatu-SPDepartamento de Bioestatistica do Instituto de Biociências da Universidade Estadual Paulista, Botucatu-SPDepartamento de Farmacologia do Instituto de Biociências da Universidade Estadual Paulista, Botucatu-SPDepartamento de Bioestatistica do Instituto de Biociências da Universidade Estadual Paulista, Botucatu-SPUniversidade Estadual Paulista (Unesp)Nabhan, Thaís [UNESP]Satrapa, Rafael Augusto [UNESP]Simões, Renato Arantes Lima [UNESP]da Silva, Cíntia Fernandes [UNESP]Razza, Eduardo Montanari [UNESP]Puelker, Raquel [UNESP]Trinca, Luzia Aparecida [UNESP]Barros, Ciro Moraes [UNESP]2014-05-27T11:26:18Z2014-05-27T11:26:18Z2011-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article336-343application/pdfhttp://www.revistas.usp.br/bjvras/article/view/34399Brazilian Journal of Veterinary Research and Animal Science, v. 48, n. 4, p. 336-343, 2011.1413-95961678-4456http://hdl.handle.net/11449/730122-s2.0-845551784242-s2.0-84555178424.pdf372048936642795577958830099878063720489366427955Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporBrazilian Journal of Veterinary Research and Animal Science0,2250,225info:eu-repo/semantics/openAccess2024-01-01T06:16:04Zoai:repositorio.unesp.br:11449/73012Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:49:51.406352Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influência da raça do touro (Bos indicus x Bos taurus) na tolerância ao estresse térmico calórico de embriões bovinos produzidos in vitro Influence of sire breed (Bos indicus vs Bos taurus) on heat stress tolerance of in vitroproduced bovine embryos |
title |
Influência da raça do touro (Bos indicus x Bos taurus) na tolerância ao estresse térmico calórico de embriões bovinos produzidos in vitro |
spellingShingle |
Influência da raça do touro (Bos indicus x Bos taurus) na tolerância ao estresse térmico calórico de embriões bovinos produzidos in vitro Nabhan, Thaís [UNESP] Blastocyst Bovine Heat stress In vitro production Bos indicus Bos taurus Bovinae |
title_short |
Influência da raça do touro (Bos indicus x Bos taurus) na tolerância ao estresse térmico calórico de embriões bovinos produzidos in vitro |
title_full |
Influência da raça do touro (Bos indicus x Bos taurus) na tolerância ao estresse térmico calórico de embriões bovinos produzidos in vitro |
title_fullStr |
Influência da raça do touro (Bos indicus x Bos taurus) na tolerância ao estresse térmico calórico de embriões bovinos produzidos in vitro |
title_full_unstemmed |
Influência da raça do touro (Bos indicus x Bos taurus) na tolerância ao estresse térmico calórico de embriões bovinos produzidos in vitro |
title_sort |
Influência da raça do touro (Bos indicus x Bos taurus) na tolerância ao estresse térmico calórico de embriões bovinos produzidos in vitro |
author |
Nabhan, Thaís [UNESP] |
author_facet |
Nabhan, Thaís [UNESP] Satrapa, Rafael Augusto [UNESP] Simões, Renato Arantes Lima [UNESP] da Silva, Cíntia Fernandes [UNESP] Razza, Eduardo Montanari [UNESP] Puelker, Raquel [UNESP] Trinca, Luzia Aparecida [UNESP] Barros, Ciro Moraes [UNESP] |
author_role |
author |
author2 |
Satrapa, Rafael Augusto [UNESP] Simões, Renato Arantes Lima [UNESP] da Silva, Cíntia Fernandes [UNESP] Razza, Eduardo Montanari [UNESP] Puelker, Raquel [UNESP] Trinca, Luzia Aparecida [UNESP] Barros, Ciro Moraes [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Nabhan, Thaís [UNESP] Satrapa, Rafael Augusto [UNESP] Simões, Renato Arantes Lima [UNESP] da Silva, Cíntia Fernandes [UNESP] Razza, Eduardo Montanari [UNESP] Puelker, Raquel [UNESP] Trinca, Luzia Aparecida [UNESP] Barros, Ciro Moraes [UNESP] |
dc.subject.por.fl_str_mv |
Blastocyst Bovine Heat stress In vitro production Bos indicus Bos taurus Bovinae |
topic |
Blastocyst Bovine Heat stress In vitro production Bos indicus Bos taurus Bovinae |
description |
To better understand the differences related to HS resistance between Bos indicus and Bos taurus, we aim to verify if the HS tolerance is due mostly to the genetic contribution from the oocyte, spermatozoa or both. Oocytes from Nelore and crossbreed Holstein cows (cHST) were collected, matured and fertilized with semen from Nelore (N), Angus (An), Brahman (Bra) and Gir (Gir) bulls. Nine six hours post insemination (hpi), ≥ 16 cells embryos were separated in two groups: control and HS. In control group, embryos were cultured at 39°C, whereas in the HS group, embryos were subjected to 41°C for 12 h, and then returned to 39°C. There was no effect of HS on blastocyst and hatched blastocyst rates in all breeds analyzed. The percentage of oocytes that cleaved and reached morula stage was significantly lower (p < 0.05) in cHST x Gir as compared to the other breeds. Additionally, blastocyst rates was higher in cHST x N than in cHST x An and cHST x Gir (p < 0.05). It was concluded that the oocyte is more important than the spermatozoa for the development of thermotolerance, since the breed of the bull did not influence embryo development after HS. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-01 2014-05-27T11:26:18Z 2014-05-27T11:26:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.revistas.usp.br/bjvras/article/view/34399 Brazilian Journal of Veterinary Research and Animal Science, v. 48, n. 4, p. 336-343, 2011. 1413-9596 1678-4456 http://hdl.handle.net/11449/73012 2-s2.0-84555178424 2-s2.0-84555178424.pdf 3720489366427955 7795883009987806 3720489366427955 |
url |
http://www.revistas.usp.br/bjvras/article/view/34399 http://hdl.handle.net/11449/73012 |
identifier_str_mv |
Brazilian Journal of Veterinary Research and Animal Science, v. 48, n. 4, p. 336-343, 2011. 1413-9596 1678-4456 2-s2.0-84555178424 2-s2.0-84555178424.pdf 3720489366427955 7795883009987806 |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science 0,225 0,225 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
336-343 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129363017728000 |