Lack of correlation between micro fungi species and chemical control method of Atta treated with toxic baits

Detalhes bibliográficos
Autor(a) principal: Barbosa, Marcílio Souza
Data de Publicação: 2018
Outros Autores: Barbosa, Suzanne Nunes, Nagamoto, Nilson Satoru [UNESP], Forti, Luiz Carlos [UNESP], Almeida, Cícero
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/0103-8478cr20170353
http://hdl.handle.net/11449/176365
Resumo: Atta sexdens rubropilosa (leaf-cutter ants) has a symbiotic association with a fungus and has a negative interaction with other fungi due to parasitism of the fungus cultivated by ants; also, there are several other fungi with no exact known role occurring in their cultivated fungus garden. In the present study, we use the ITS region (internal transcribed spacer) to identify fungi in colonies treated with toxic baits. Experiments using two toxic baits were carried out: 0.75g of sulfluramid [0.3%] and 0.75g fipronil [0.003%]. Samples of fungi were collected and cultured in Czapek medium for seven days to allow fungal growth and subsequent identification. Total DNA was isolated from 100-150 mg of mycelium using the CTAB method and using PCR, with the universal primers (ITS4 and ITS5), to amplify the ITS region. Sequencing was performed using the Sanger method. Sequences were subjected to BLAST, allowing the identification of nine different species of the orders Agaricales, Eurotiales, Hypocreales, Pleosporales, Saccharomycetales and Tremellales showing a variation in identity of 96- 100%. Using “The Automatic Barcode Gap Discovery” analysis, nine groups were identified, corresponding to species described in NCBI. The K2P distances were used to generate a tree using Neighbour-joining, demonstrating that the species were grouped according to phylogenetic groups. We concluded that leaf-cutter ant colonies exhibited a wide variety of fungi and this study suggested that there is no correlation between the species of fungi isolated with the control method used on the ant nest.
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spelling Lack of correlation between micro fungi species and chemical control method of Atta treated with toxic baitsAusência de correlação entre espécies de microfungos e método de controle químico de Atta tratadas com iscas tóxicasAntsDNA barcodingITSLeaf-cutterAtta sexdens rubropilosa (leaf-cutter ants) has a symbiotic association with a fungus and has a negative interaction with other fungi due to parasitism of the fungus cultivated by ants; also, there are several other fungi with no exact known role occurring in their cultivated fungus garden. In the present study, we use the ITS region (internal transcribed spacer) to identify fungi in colonies treated with toxic baits. Experiments using two toxic baits were carried out: 0.75g of sulfluramid [0.3%] and 0.75g fipronil [0.003%]. Samples of fungi were collected and cultured in Czapek medium for seven days to allow fungal growth and subsequent identification. Total DNA was isolated from 100-150 mg of mycelium using the CTAB method and using PCR, with the universal primers (ITS4 and ITS5), to amplify the ITS region. Sequencing was performed using the Sanger method. Sequences were subjected to BLAST, allowing the identification of nine different species of the orders Agaricales, Eurotiales, Hypocreales, Pleosporales, Saccharomycetales and Tremellales showing a variation in identity of 96- 100%. Using “The Automatic Barcode Gap Discovery” analysis, nine groups were identified, corresponding to species described in NCBI. The K2P distances were used to generate a tree using Neighbour-joining, demonstrating that the species were grouped according to phylogenetic groups. We concluded that leaf-cutter ant colonies exhibited a wide variety of fungi and this study suggested that there is no correlation between the species of fungi isolated with the control method used on the ant nest.Instituto Federal de Alagoas (IFAL), Campus Piranhas, Av Sergipe, s/n, XingóLaboratório de Recursos Genéticos Universidade Federal de Alagoas (UFAL), Campus de ArapiracaLaboratório de Insetos Sociais-Praga Faculdade de Ciências Agronômicas Universidade Estadual Paulista (UNESP)Laboratório de Insetos Sociais-Praga Faculdade de Ciências Agronômicas Universidade Estadual Paulista (UNESP)Instituto Federal de Alagoas (IFAL)Universidade Federal de Alagoas (UFAL)Universidade Estadual Paulista (Unesp)Barbosa, Marcílio SouzaBarbosa, Suzanne NunesNagamoto, Nilson Satoru [UNESP]Forti, Luiz Carlos [UNESP]Almeida, Cícero2018-12-11T17:20:30Z2018-12-11T17:20:30Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1590/0103-8478cr20170353Ciencia Rural, v. 48, n. 5, 2018.1678-45960103-8478http://hdl.handle.net/11449/17636510.1590/0103-8478cr20170353S0103-847820180005001502-s2.0-85047435548S0103-84782018000500150.pdf6187684824965648Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCiencia Rural0,337info:eu-repo/semantics/openAccess2024-04-11T14:57:11Zoai:repositorio.unesp.br:11449/176365Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:19:42.335706Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Lack of correlation between micro fungi species and chemical control method of Atta treated with toxic baits
Ausência de correlação entre espécies de microfungos e método de controle químico de Atta tratadas com iscas tóxicas
title Lack of correlation between micro fungi species and chemical control method of Atta treated with toxic baits
spellingShingle Lack of correlation between micro fungi species and chemical control method of Atta treated with toxic baits
Barbosa, Marcílio Souza
Ants
DNA barcoding
ITS
Leaf-cutter
title_short Lack of correlation between micro fungi species and chemical control method of Atta treated with toxic baits
title_full Lack of correlation between micro fungi species and chemical control method of Atta treated with toxic baits
title_fullStr Lack of correlation between micro fungi species and chemical control method of Atta treated with toxic baits
title_full_unstemmed Lack of correlation between micro fungi species and chemical control method of Atta treated with toxic baits
title_sort Lack of correlation between micro fungi species and chemical control method of Atta treated with toxic baits
author Barbosa, Marcílio Souza
author_facet Barbosa, Marcílio Souza
Barbosa, Suzanne Nunes
Nagamoto, Nilson Satoru [UNESP]
Forti, Luiz Carlos [UNESP]
Almeida, Cícero
author_role author
author2 Barbosa, Suzanne Nunes
Nagamoto, Nilson Satoru [UNESP]
Forti, Luiz Carlos [UNESP]
Almeida, Cícero
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Instituto Federal de Alagoas (IFAL)
Universidade Federal de Alagoas (UFAL)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Barbosa, Marcílio Souza
Barbosa, Suzanne Nunes
Nagamoto, Nilson Satoru [UNESP]
Forti, Luiz Carlos [UNESP]
Almeida, Cícero
dc.subject.por.fl_str_mv Ants
DNA barcoding
ITS
Leaf-cutter
topic Ants
DNA barcoding
ITS
Leaf-cutter
description Atta sexdens rubropilosa (leaf-cutter ants) has a symbiotic association with a fungus and has a negative interaction with other fungi due to parasitism of the fungus cultivated by ants; also, there are several other fungi with no exact known role occurring in their cultivated fungus garden. In the present study, we use the ITS region (internal transcribed spacer) to identify fungi in colonies treated with toxic baits. Experiments using two toxic baits were carried out: 0.75g of sulfluramid [0.3%] and 0.75g fipronil [0.003%]. Samples of fungi were collected and cultured in Czapek medium for seven days to allow fungal growth and subsequent identification. Total DNA was isolated from 100-150 mg of mycelium using the CTAB method and using PCR, with the universal primers (ITS4 and ITS5), to amplify the ITS region. Sequencing was performed using the Sanger method. Sequences were subjected to BLAST, allowing the identification of nine different species of the orders Agaricales, Eurotiales, Hypocreales, Pleosporales, Saccharomycetales and Tremellales showing a variation in identity of 96- 100%. Using “The Automatic Barcode Gap Discovery” analysis, nine groups were identified, corresponding to species described in NCBI. The K2P distances were used to generate a tree using Neighbour-joining, demonstrating that the species were grouped according to phylogenetic groups. We concluded that leaf-cutter ant colonies exhibited a wide variety of fungi and this study suggested that there is no correlation between the species of fungi isolated with the control method used on the ant nest.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:20:30Z
2018-12-11T17:20:30Z
2018-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/0103-8478cr20170353
Ciencia Rural, v. 48, n. 5, 2018.
1678-4596
0103-8478
http://hdl.handle.net/11449/176365
10.1590/0103-8478cr20170353
S0103-84782018000500150
2-s2.0-85047435548
S0103-84782018000500150.pdf
6187684824965648
url http://dx.doi.org/10.1590/0103-8478cr20170353
http://hdl.handle.net/11449/176365
identifier_str_mv Ciencia Rural, v. 48, n. 5, 2018.
1678-4596
0103-8478
10.1590/0103-8478cr20170353
S0103-84782018000500150
2-s2.0-85047435548
S0103-84782018000500150.pdf
6187684824965648
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ciencia Rural
0,337
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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