Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0095662 http://hdl.handle.net/11449/111624 |
Resumo: | Background: It is known that physico/chemical alterations on biomaterial surfaces have the capability to modulate cellular behavior, affecting early tissue repair. Such surface modifications are aimed to improve early healing response and, clinically, offer the possibility to shorten the time from implant placement to functional loading. Since FAK and Src are intracellular proteins able to predict the quality of osteoblast adhesion, this study evaluated the osteoblast behavior in response to nanometer scale titanium surface texturing by monitoring FAK and Src phosphorylations.Methodology: Four engineered titanium surfaces were used for the study: machined (M), dual acid-etched (DAA), resorbable media microblasted and acid-etched (MBAA), and acid-etch microblasted (AAMB). Surfaces were characterized by scanning electron microscopy, interferometry, atomic force microscopy, x-ray photoelectron spectroscopy and energy dispersive X-ray spectroscopy. Thereafter, those 4 samples were used to evaluate their cytotoxicity and interference on FAK and Src phosphorylations. Both Src and FAK were investigated by using specific antibody against specific phosphorylation sites.Principal Findings: The results showed that both FAK and Src activations were differently modulated as a function of titanium surfaces physico/chemical configuration and protein adsorption.Conclusions: It can be suggested that signaling pathways involving both FAK and Src could provide biomarkers to predict osteoblast adhesion onto different surfaces. |
id |
UNSP_cfcdf31567ef248b5876187410d091af |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/111624 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src ActivationsBackground: It is known that physico/chemical alterations on biomaterial surfaces have the capability to modulate cellular behavior, affecting early tissue repair. Such surface modifications are aimed to improve early healing response and, clinically, offer the possibility to shorten the time from implant placement to functional loading. Since FAK and Src are intracellular proteins able to predict the quality of osteoblast adhesion, this study evaluated the osteoblast behavior in response to nanometer scale titanium surface texturing by monitoring FAK and Src phosphorylations.Methodology: Four engineered titanium surfaces were used for the study: machined (M), dual acid-etched (DAA), resorbable media microblasted and acid-etched (MBAA), and acid-etch microblasted (AAMB). Surfaces were characterized by scanning electron microscopy, interferometry, atomic force microscopy, x-ray photoelectron spectroscopy and energy dispersive X-ray spectroscopy. Thereafter, those 4 samples were used to evaluate their cytotoxicity and interference on FAK and Src phosphorylations. Both Src and FAK were investigated by using specific antibody against specific phosphorylation sites.Principal Findings: The results showed that both FAK and Src activations were differently modulated as a function of titanium surfaces physico/chemical configuration and protein adsorption.Conclusions: It can be suggested that signaling pathways involving both FAK and Src could provide biomarkers to predict osteoblast adhesion onto different surfaces.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Univ Estadual Paulista, UNESP, Inst Biociencias, Dept Quim & Bioquim, Sao Paulo, BrazilUniv Sao Paulo, Fac Odontol Bauru, Sao Paulo, BrazilMalmo Univ, Fac Odontol, Dept Prosthodont, Malmo, SwedenChalmers, Dept Chem & Biol Engn, S-41296 Gothenburg, SwedenUniv Fed Fluminense, Dept Cell & Mol Biol, Inst Biol, Niteroi, RJ, BrazilExcell Biomed Serv, Rio De Janeiro, BrazilNatl Inst Metrol Qual & Technol INMETRO, Rio De Janeiro, BrazilNYU, Coll Dent, Dept Biomat & Biomimet, New York, NY USAUniv Estadual Paulista, UNESP, Inst Biociencias, Dept Quim & Bioquim, Sao Paulo, BrazilPublic Library ScienceUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Malmo UnivChalmersUniversidade Federal Fluminense (UFF)Excell Biomed ServNatl Inst Metrol Qual & Technol INMETRONYUZambuzzi, Willian F. [UNESP]Bonfante, Estevam A.Jimbo, RyoHayashi, MarikoAndersson, MartinAlves, GutembergTakamori, Esther R.Beltrao, Paulo J.Coelho, Paulo G.Granjeiro, Jose M.2014-12-03T13:08:50Z2014-12-03T13:08:50Z2014-07-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://dx.doi.org/10.1371/journal.pone.0095662Plos One. San Francisco: Public Library Science, v. 9, n. 7, 11 p., 2014.1932-6203http://hdl.handle.net/11449/11162410.1371/journal.pone.0095662WOS:000338637300002WOS000338637300002.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLOS ONE2.7661,164info:eu-repo/semantics/openAccess2023-12-09T06:18:24Zoai:repositorio.unesp.br:11449/111624Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:50:19.809533Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations |
title |
Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations |
spellingShingle |
Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations Zambuzzi, Willian F. [UNESP] |
title_short |
Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations |
title_full |
Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations |
title_fullStr |
Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations |
title_full_unstemmed |
Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations |
title_sort |
Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations |
author |
Zambuzzi, Willian F. [UNESP] |
author_facet |
Zambuzzi, Willian F. [UNESP] Bonfante, Estevam A. Jimbo, Ryo Hayashi, Mariko Andersson, Martin Alves, Gutemberg Takamori, Esther R. Beltrao, Paulo J. Coelho, Paulo G. Granjeiro, Jose M. |
author_role |
author |
author2 |
Bonfante, Estevam A. Jimbo, Ryo Hayashi, Mariko Andersson, Martin Alves, Gutemberg Takamori, Esther R. Beltrao, Paulo J. Coelho, Paulo G. Granjeiro, Jose M. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Malmo Univ Chalmers Universidade Federal Fluminense (UFF) Excell Biomed Serv Natl Inst Metrol Qual & Technol INMETRO NYU |
dc.contributor.author.fl_str_mv |
Zambuzzi, Willian F. [UNESP] Bonfante, Estevam A. Jimbo, Ryo Hayashi, Mariko Andersson, Martin Alves, Gutemberg Takamori, Esther R. Beltrao, Paulo J. Coelho, Paulo G. Granjeiro, Jose M. |
description |
Background: It is known that physico/chemical alterations on biomaterial surfaces have the capability to modulate cellular behavior, affecting early tissue repair. Such surface modifications are aimed to improve early healing response and, clinically, offer the possibility to shorten the time from implant placement to functional loading. Since FAK and Src are intracellular proteins able to predict the quality of osteoblast adhesion, this study evaluated the osteoblast behavior in response to nanometer scale titanium surface texturing by monitoring FAK and Src phosphorylations.Methodology: Four engineered titanium surfaces were used for the study: machined (M), dual acid-etched (DAA), resorbable media microblasted and acid-etched (MBAA), and acid-etch microblasted (AAMB). Surfaces were characterized by scanning electron microscopy, interferometry, atomic force microscopy, x-ray photoelectron spectroscopy and energy dispersive X-ray spectroscopy. Thereafter, those 4 samples were used to evaluate their cytotoxicity and interference on FAK and Src phosphorylations. Both Src and FAK were investigated by using specific antibody against specific phosphorylation sites.Principal Findings: The results showed that both FAK and Src activations were differently modulated as a function of titanium surfaces physico/chemical configuration and protein adsorption.Conclusions: It can be suggested that signaling pathways involving both FAK and Src could provide biomarkers to predict osteoblast adhesion onto different surfaces. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-03T13:08:50Z 2014-12-03T13:08:50Z 2014-07-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0095662 Plos One. San Francisco: Public Library Science, v. 9, n. 7, 11 p., 2014. 1932-6203 http://hdl.handle.net/11449/111624 10.1371/journal.pone.0095662 WOS:000338637300002 WOS000338637300002.pdf |
url |
http://dx.doi.org/10.1371/journal.pone.0095662 http://hdl.handle.net/11449/111624 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 9, n. 7, 11 p., 2014. 1932-6203 10.1371/journal.pone.0095662 WOS:000338637300002 WOS000338637300002.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLOS ONE 2.766 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129126360416256 |