The importance of the assembling of DNA strands on the performance of electrochemical genosensors
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.microc.2020.105358 http://hdl.handle.net/11449/200859 |
Resumo: | Electrochemical biosensors have been extensively studied due to their capacity for rapid and accurate detection of a wide variety of target molecules or biomarkers. Impedimetric DNA hybridization sensors are based on tracking negative charge variation over the electrode surface owing to the target sequence of hybridization to the single-stranded immobilized DNA probes. However, the development of this platform requires an understanding of how to control the immobilization and the structure formed on the interface. The DNA assembling, as is the case of the immobilization of the single-stranded DNA on the electrode surface and the subsequent hybridization, is crucial for the performance of the biosensor and is dependent on the solution environment characteristics, as we evidenced here. Particularly, the influence of DNA probe preparation and immobilization on gold surfaces were investigated using different protocols. The influence of negatively charged redox couple in solution was studied by impedance spectroscopy and we succeed in obtaining stable and reproducible results using target sequences from 10 pmol L−1 to 1 mmol L−1. We also verified that the ionic strength of the buffer has a strong influence over the immobilization process and sensor performance. |
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The importance of the assembling of DNA strands on the performance of electrochemical genosensorsDNA BiosensorElectrochemical sensorsImpedanceMolecular assemblyElectrochemical biosensors have been extensively studied due to their capacity for rapid and accurate detection of a wide variety of target molecules or biomarkers. Impedimetric DNA hybridization sensors are based on tracking negative charge variation over the electrode surface owing to the target sequence of hybridization to the single-stranded immobilized DNA probes. However, the development of this platform requires an understanding of how to control the immobilization and the structure formed on the interface. The DNA assembling, as is the case of the immobilization of the single-stranded DNA on the electrode surface and the subsequent hybridization, is crucial for the performance of the biosensor and is dependent on the solution environment characteristics, as we evidenced here. Particularly, the influence of DNA probe preparation and immobilization on gold surfaces were investigated using different protocols. The influence of negatively charged redox couple in solution was studied by impedance spectroscopy and we succeed in obtaining stable and reproducible results using target sequences from 10 pmol L−1 to 1 mmol L−1. We also verified that the ionic strength of the buffer has a strong influence over the immobilization process and sensor performance.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Physics Faculty of Philosophy Sciences and Letters University of São Paulo USPDepartment of Physical Chemistry Institute of Chemistry São Paulo State University UNESPDepartment of Physical Chemistry Institute of Chemistry São Paulo State University UNESPFAPESP: 2014/09562-4FAPESP: 2015/14403-5FAPESP: 2017/24839-0Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Batistuti, Marina R.Bueno, Paulo R. [UNESP]Mulato, Marcelo2020-12-12T02:18:01Z2020-12-12T02:18:01Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.microc.2020.105358Microchemical Journal, v. 159.0026-265Xhttp://hdl.handle.net/11449/20085910.1016/j.microc.2020.1053582-s2.0-85089080005Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrochemical Journalinfo:eu-repo/semantics/openAccess2021-10-23T15:25:29Zoai:repositorio.unesp.br:11449/200859Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:32:05.985496Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The importance of the assembling of DNA strands on the performance of electrochemical genosensors |
title |
The importance of the assembling of DNA strands on the performance of electrochemical genosensors |
spellingShingle |
The importance of the assembling of DNA strands on the performance of electrochemical genosensors Batistuti, Marina R. DNA Biosensor Electrochemical sensors Impedance Molecular assembly |
title_short |
The importance of the assembling of DNA strands on the performance of electrochemical genosensors |
title_full |
The importance of the assembling of DNA strands on the performance of electrochemical genosensors |
title_fullStr |
The importance of the assembling of DNA strands on the performance of electrochemical genosensors |
title_full_unstemmed |
The importance of the assembling of DNA strands on the performance of electrochemical genosensors |
title_sort |
The importance of the assembling of DNA strands on the performance of electrochemical genosensors |
author |
Batistuti, Marina R. |
author_facet |
Batistuti, Marina R. Bueno, Paulo R. [UNESP] Mulato, Marcelo |
author_role |
author |
author2 |
Bueno, Paulo R. [UNESP] Mulato, Marcelo |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Batistuti, Marina R. Bueno, Paulo R. [UNESP] Mulato, Marcelo |
dc.subject.por.fl_str_mv |
DNA Biosensor Electrochemical sensors Impedance Molecular assembly |
topic |
DNA Biosensor Electrochemical sensors Impedance Molecular assembly |
description |
Electrochemical biosensors have been extensively studied due to their capacity for rapid and accurate detection of a wide variety of target molecules or biomarkers. Impedimetric DNA hybridization sensors are based on tracking negative charge variation over the electrode surface owing to the target sequence of hybridization to the single-stranded immobilized DNA probes. However, the development of this platform requires an understanding of how to control the immobilization and the structure formed on the interface. The DNA assembling, as is the case of the immobilization of the single-stranded DNA on the electrode surface and the subsequent hybridization, is crucial for the performance of the biosensor and is dependent on the solution environment characteristics, as we evidenced here. Particularly, the influence of DNA probe preparation and immobilization on gold surfaces were investigated using different protocols. The influence of negatively charged redox couple in solution was studied by impedance spectroscopy and we succeed in obtaining stable and reproducible results using target sequences from 10 pmol L−1 to 1 mmol L−1. We also verified that the ionic strength of the buffer has a strong influence over the immobilization process and sensor performance. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:18:01Z 2020-12-12T02:18:01Z 2020-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.microc.2020.105358 Microchemical Journal, v. 159. 0026-265X http://hdl.handle.net/11449/200859 10.1016/j.microc.2020.105358 2-s2.0-85089080005 |
url |
http://dx.doi.org/10.1016/j.microc.2020.105358 http://hdl.handle.net/11449/200859 |
identifier_str_mv |
Microchemical Journal, v. 159. 0026-265X 10.1016/j.microc.2020.105358 2-s2.0-85089080005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Microchemical Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129434687897600 |