Aspectos fisiológicos, celulares e moleculares da tolerância à dessecação em sementes de Anadenanthera colubrina durante a germinação
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1519-6984.00616 http://hdl.handle.net/11449/170236 |
Resumo: | During germination, orthodox seeds become gradually intolerant to desiccation, and for this reason, they are a good model for recalcitrance studies. In the present work, physiological, biochemical, and ultrastructural aspects of the desiccation tolerance were characterized during the germination process of Anadenanthera colubrina seeds. The seeds were imbibed during zero (control), 2, 8, 12 (no germinated seeds), and 18 hours (germinated seeds with 1 mm protruded radicle); then they were dried for 72 hours, rehydrated and evaluated for survivorship. Along the imbibition, cytometric and ultrastructural analysis were performed, besides the extraction of the heat-stable proteins. Posteriorly to imbibition and drying, the evaluation of ultrastructural damages was performed. Desiccation tolerance was fully lost after root protrusion. There was no increase in 4C DNA content after the loss of desiccation tolerance. Ultrastructural characteristics of cells from 1mm roots resembled those found in the recalcitrant seeds, in both hydrated and dehydrated states. The loss of desiccation tolerance coincided with the reduction of heat-stable proteins. |
id |
UNSP_d15faf7ac64b4bcf1d2cc6c192b7848c |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/170236 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Aspectos fisiológicos, celulares e moleculares da tolerância à dessecação em sementes de Anadenanthera colubrina durante a germinaçãoPhysiological, cellular and molecular aspects of the desiccation tolerance in Anadenanthera colubrina seeds during germinationDesiccation toleranceDryingForest seedsDuring germination, orthodox seeds become gradually intolerant to desiccation, and for this reason, they are a good model for recalcitrance studies. In the present work, physiological, biochemical, and ultrastructural aspects of the desiccation tolerance were characterized during the germination process of Anadenanthera colubrina seeds. The seeds were imbibed during zero (control), 2, 8, 12 (no germinated seeds), and 18 hours (germinated seeds with 1 mm protruded radicle); then they were dried for 72 hours, rehydrated and evaluated for survivorship. Along the imbibition, cytometric and ultrastructural analysis were performed, besides the extraction of the heat-stable proteins. Posteriorly to imbibition and drying, the evaluation of ultrastructural damages was performed. Desiccation tolerance was fully lost after root protrusion. There was no increase in 4C DNA content after the loss of desiccation tolerance. Ultrastructural characteristics of cells from 1mm roots resembled those found in the recalcitrant seeds, in both hydrated and dehydrated states. The loss of desiccation tolerance coincided with the reduction of heat-stable proteins.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Instituto de Biociências Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP, Avenida 24-A, 1515, Bairro Bela VistaDepartment of Agriculture Universidade Federal de Lavras – UFLA, CP 3037Laboratory of Tree Seeds Department of Forest Sciences Universidade Federal de Lavras – UFLA, CP 3037Instituto de Biociências Universidade Estadual Paulista “Júlio de Mesquita Filho” – UNESP, Avenida 24-A, 1515, Bairro Bela VistaUniversidade Estadual Paulista (Unesp)Universidade Federal de Lavras (UFLA)Castro, L. E. [UNESP]Guimarães, C. C.Faria, J. M.R.2018-12-11T16:49:53Z2018-12-11T16:49:53Z2017-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article774-780application/pdfhttp://dx.doi.org/10.1590/1519-6984.00616Brazilian Journal of Biology, v. 77, n. 4, p. 774-780, 2017.1678-43751519-6984http://hdl.handle.net/11449/17023610.1590/1519-6984.00616S1519-698420170004007742-s2.0-85030684616S1519-69842017000400774.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Biology0,523info:eu-repo/semantics/openAccess2023-12-22T06:21:17Zoai:repositorio.unesp.br:11449/170236Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-22T06:21:17Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Aspectos fisiológicos, celulares e moleculares da tolerância à dessecação em sementes de Anadenanthera colubrina durante a germinação Physiological, cellular and molecular aspects of the desiccation tolerance in Anadenanthera colubrina seeds during germination |
title |
Aspectos fisiológicos, celulares e moleculares da tolerância à dessecação em sementes de Anadenanthera colubrina durante a germinação |
spellingShingle |
Aspectos fisiológicos, celulares e moleculares da tolerância à dessecação em sementes de Anadenanthera colubrina durante a germinação Castro, L. E. [UNESP] Desiccation tolerance Drying Forest seeds |
title_short |
Aspectos fisiológicos, celulares e moleculares da tolerância à dessecação em sementes de Anadenanthera colubrina durante a germinação |
title_full |
Aspectos fisiológicos, celulares e moleculares da tolerância à dessecação em sementes de Anadenanthera colubrina durante a germinação |
title_fullStr |
Aspectos fisiológicos, celulares e moleculares da tolerância à dessecação em sementes de Anadenanthera colubrina durante a germinação |
title_full_unstemmed |
Aspectos fisiológicos, celulares e moleculares da tolerância à dessecação em sementes de Anadenanthera colubrina durante a germinação |
title_sort |
Aspectos fisiológicos, celulares e moleculares da tolerância à dessecação em sementes de Anadenanthera colubrina durante a germinação |
author |
Castro, L. E. [UNESP] |
author_facet |
Castro, L. E. [UNESP] Guimarães, C. C. Faria, J. M.R. |
author_role |
author |
author2 |
Guimarães, C. C. Faria, J. M.R. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Lavras (UFLA) |
dc.contributor.author.fl_str_mv |
Castro, L. E. [UNESP] Guimarães, C. C. Faria, J. M.R. |
dc.subject.por.fl_str_mv |
Desiccation tolerance Drying Forest seeds |
topic |
Desiccation tolerance Drying Forest seeds |
description |
During germination, orthodox seeds become gradually intolerant to desiccation, and for this reason, they are a good model for recalcitrance studies. In the present work, physiological, biochemical, and ultrastructural aspects of the desiccation tolerance were characterized during the germination process of Anadenanthera colubrina seeds. The seeds were imbibed during zero (control), 2, 8, 12 (no germinated seeds), and 18 hours (germinated seeds with 1 mm protruded radicle); then they were dried for 72 hours, rehydrated and evaluated for survivorship. Along the imbibition, cytometric and ultrastructural analysis were performed, besides the extraction of the heat-stable proteins. Posteriorly to imbibition and drying, the evaluation of ultrastructural damages was performed. Desiccation tolerance was fully lost after root protrusion. There was no increase in 4C DNA content after the loss of desiccation tolerance. Ultrastructural characteristics of cells from 1mm roots resembled those found in the recalcitrant seeds, in both hydrated and dehydrated states. The loss of desiccation tolerance coincided with the reduction of heat-stable proteins. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-01 2018-12-11T16:49:53Z 2018-12-11T16:49:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1519-6984.00616 Brazilian Journal of Biology, v. 77, n. 4, p. 774-780, 2017. 1678-4375 1519-6984 http://hdl.handle.net/11449/170236 10.1590/1519-6984.00616 S1519-69842017000400774 2-s2.0-85030684616 S1519-69842017000400774.pdf |
url |
http://dx.doi.org/10.1590/1519-6984.00616 http://hdl.handle.net/11449/170236 |
identifier_str_mv |
Brazilian Journal of Biology, v. 77, n. 4, p. 774-780, 2017. 1678-4375 1519-6984 10.1590/1519-6984.00616 S1519-69842017000400774 2-s2.0-85030684616 S1519-69842017000400774.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Biology 0,523 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
774-780 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790075156692992 |