Study of Bacillus spp. strains used in the production of γ-polyglutamic acid in submerged culture
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/221531 |
Resumo: | Gamma-polyglutamic (γ-polyglutamic) acid has received considerable attention for environmental applications. γ-polyglutamic acid can be produced by fermenting soybeans and by submerged culture using the bacterium Bacillus subtilis. Although it is practically impossible to chemically synthesise γ-polyglutamic acid, this biopolymer can be obtained through microbial culture. The microorganism most commonly used in the production of γ-polyglutamic acid, among other strains, is B. subtilis, which has been investigated for the production of biopolymers. The purpose of the present work was to evaluate the production of γ-polyglutamic acid from submerged culture of different Bacillus strains. The evaluation of the efficiency of Bacillus strains in producing γ-polyglutamic acid indicated that B. licheniformis produced a higher volume of the biopolymer than B. subtilis. The highest γ-polyglutamic acid production by B. licheniformis was 398.1 mg L-1. B. licheniformis was found to be an L-glutamic acid-independent bacterium for the production of γ-polyglutamic acid. B. licheniformis and B. subtilis growth and γ-polyglutamic acid production rates were also influenced by the culture media. |
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Study of Bacillus spp. strains used in the production of γ-polyglutamic acid in submerged cultureGenus bacillusMicroorganismsSoybean fermentationSubmerged cultureγ-polyglutamic acidGamma-polyglutamic (γ-polyglutamic) acid has received considerable attention for environmental applications. γ-polyglutamic acid can be produced by fermenting soybeans and by submerged culture using the bacterium Bacillus subtilis. Although it is practically impossible to chemically synthesise γ-polyglutamic acid, this biopolymer can be obtained through microbial culture. The microorganism most commonly used in the production of γ-polyglutamic acid, among other strains, is B. subtilis, which has been investigated for the production of biopolymers. The purpose of the present work was to evaluate the production of γ-polyglutamic acid from submerged culture of different Bacillus strains. The evaluation of the efficiency of Bacillus strains in producing γ-polyglutamic acid indicated that B. licheniformis produced a higher volume of the biopolymer than B. subtilis. The highest γ-polyglutamic acid production by B. licheniformis was 398.1 mg L-1. B. licheniformis was found to be an L-glutamic acid-independent bacterium for the production of γ-polyglutamic acid. B. licheniformis and B. subtilis growth and γ-polyglutamic acid production rates were also influenced by the culture media.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Science and Technology Institute of Sorocaba (ICTS) São Paulo State UniversityCenter of Nature Science Universidade Federal de São CarlosScience and Technology Institute of Sorocaba (ICTS) São Paulo State UniversityCNPq: 400040/2016-6Universidade Estadual Paulista (UNESP)Universidade Federal de São Carlos (UFSCar)Campos, V. [UNESP]Pimentel Filho, N. J.Medeiros, T. A.M. [UNESP]2022-04-28T19:29:15Z2022-04-28T19:29:15Z2020-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article427-434International Food Research Journal, v. 27, n. 3, p. 427-434, 2020.2231-75461985-4668http://hdl.handle.net/11449/2215312-s2.0-85089262400Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Food Research Journalinfo:eu-repo/semantics/openAccess2022-04-28T19:29:15Zoai:repositorio.unesp.br:11449/221531Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:44:51.868104Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Study of Bacillus spp. strains used in the production of γ-polyglutamic acid in submerged culture |
title |
Study of Bacillus spp. strains used in the production of γ-polyglutamic acid in submerged culture |
spellingShingle |
Study of Bacillus spp. strains used in the production of γ-polyglutamic acid in submerged culture Campos, V. [UNESP] Genus bacillus Microorganisms Soybean fermentation Submerged culture γ-polyglutamic acid |
title_short |
Study of Bacillus spp. strains used in the production of γ-polyglutamic acid in submerged culture |
title_full |
Study of Bacillus spp. strains used in the production of γ-polyglutamic acid in submerged culture |
title_fullStr |
Study of Bacillus spp. strains used in the production of γ-polyglutamic acid in submerged culture |
title_full_unstemmed |
Study of Bacillus spp. strains used in the production of γ-polyglutamic acid in submerged culture |
title_sort |
Study of Bacillus spp. strains used in the production of γ-polyglutamic acid in submerged culture |
author |
Campos, V. [UNESP] |
author_facet |
Campos, V. [UNESP] Pimentel Filho, N. J. Medeiros, T. A.M. [UNESP] |
author_role |
author |
author2 |
Pimentel Filho, N. J. Medeiros, T. A.M. [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Campos, V. [UNESP] Pimentel Filho, N. J. Medeiros, T. A.M. [UNESP] |
dc.subject.por.fl_str_mv |
Genus bacillus Microorganisms Soybean fermentation Submerged culture γ-polyglutamic acid |
topic |
Genus bacillus Microorganisms Soybean fermentation Submerged culture γ-polyglutamic acid |
description |
Gamma-polyglutamic (γ-polyglutamic) acid has received considerable attention for environmental applications. γ-polyglutamic acid can be produced by fermenting soybeans and by submerged culture using the bacterium Bacillus subtilis. Although it is practically impossible to chemically synthesise γ-polyglutamic acid, this biopolymer can be obtained through microbial culture. The microorganism most commonly used in the production of γ-polyglutamic acid, among other strains, is B. subtilis, which has been investigated for the production of biopolymers. The purpose of the present work was to evaluate the production of γ-polyglutamic acid from submerged culture of different Bacillus strains. The evaluation of the efficiency of Bacillus strains in producing γ-polyglutamic acid indicated that B. licheniformis produced a higher volume of the biopolymer than B. subtilis. The highest γ-polyglutamic acid production by B. licheniformis was 398.1 mg L-1. B. licheniformis was found to be an L-glutamic acid-independent bacterium for the production of γ-polyglutamic acid. B. licheniformis and B. subtilis growth and γ-polyglutamic acid production rates were also influenced by the culture media. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-06-01 2022-04-28T19:29:15Z 2022-04-28T19:29:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
International Food Research Journal, v. 27, n. 3, p. 427-434, 2020. 2231-7546 1985-4668 http://hdl.handle.net/11449/221531 2-s2.0-85089262400 |
identifier_str_mv |
International Food Research Journal, v. 27, n. 3, p. 427-434, 2020. 2231-7546 1985-4668 2-s2.0-85089262400 |
url |
http://hdl.handle.net/11449/221531 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Food Research Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
427-434 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128973424558080 |