Expression profiles of Annexin A1, formylated peptide receptors and cyclooxigenase-2 in gastroesophageal inflammations and neoplasias
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.prp.2017.12.003 http://hdl.handle.net/11449/175694 |
Resumo: | The anti−inflammatory protein Annexin−A1 (ANXA1) is associated to tumor invasion process and its actions can be mediated by formylated peptides receptors (FPRs). Therefore, we evaluated the expression and correlation of ANXA1, FPR and cyclooxygenase−2 (COX−2) enzyme in esophageal and stomach inflammations and neoplasias. The study of proteins was performed by immunohistochemistry in biopsies of esophagitis, Barrett's esophagus, squamous cell carcinoma and adenocarcinoma of the esophagus, as well as gastritis, stomach polypus and gastric adenocarcinoma. The intensity of the expressions was evaluated by densitometry. The immunohistochemical and densitometric analyzes showed specificity for the FPR1 receptor and modulation of the ANXA1, COX−2 and FPR1 expressions in the epithelial cells in the different studied conditions. Increased immunoreactivity of these proteins was observed in cases of inflammation and stomach polypus. Interestingly, moderate immunoreactivity for ANXA1 and FPR1 but increased immunolabeling for COX−2 were observed in Barrett́s esophagus and esophageal adenocarcinomas. Also, there was reduced expression of ANXA1 and FPR1 in esophageal carcinoma but COX−2 overexpression in this tumor. There was no expression of FPR2 but ANXA1 and FPR1 expressions were positively correlated in all clinical conditions studied. Positive correlation between ANXA1 and COX−2 were also observed in inflammation conditions while negative correlation between ANXA1 and COX−2 was observed in esophageal carcinoma. Our results demonstrate the unregulated expression of ANXA1 and COX−2 in precursor lesions of esophageal and stomach cancers, reinforcing their involvement in gastroesophageal carcinogenesis. In addition, the data show that the actions of ANXA1 in the inflammatory and neoplastic processes of the esophagus and stomach are specifically mediated by the FPR1 receptor. |
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Expression profiles of Annexin A1, formylated peptide receptors and cyclooxigenase-2 in gastroesophageal inflammations and neoplasiasANXA1FPRInflammationTumorsUpper digestive tractThe anti−inflammatory protein Annexin−A1 (ANXA1) is associated to tumor invasion process and its actions can be mediated by formylated peptides receptors (FPRs). Therefore, we evaluated the expression and correlation of ANXA1, FPR and cyclooxygenase−2 (COX−2) enzyme in esophageal and stomach inflammations and neoplasias. The study of proteins was performed by immunohistochemistry in biopsies of esophagitis, Barrett's esophagus, squamous cell carcinoma and adenocarcinoma of the esophagus, as well as gastritis, stomach polypus and gastric adenocarcinoma. The intensity of the expressions was evaluated by densitometry. The immunohistochemical and densitometric analyzes showed specificity for the FPR1 receptor and modulation of the ANXA1, COX−2 and FPR1 expressions in the epithelial cells in the different studied conditions. Increased immunoreactivity of these proteins was observed in cases of inflammation and stomach polypus. Interestingly, moderate immunoreactivity for ANXA1 and FPR1 but increased immunolabeling for COX−2 were observed in Barrett́s esophagus and esophageal adenocarcinomas. Also, there was reduced expression of ANXA1 and FPR1 in esophageal carcinoma but COX−2 overexpression in this tumor. There was no expression of FPR2 but ANXA1 and FPR1 expressions were positively correlated in all clinical conditions studied. Positive correlation between ANXA1 and COX−2 were also observed in inflammation conditions while negative correlation between ANXA1 and COX−2 was observed in esophageal carcinoma. Our results demonstrate the unregulated expression of ANXA1 and COX−2 in precursor lesions of esophageal and stomach cancers, reinforcing their involvement in gastroesophageal carcinogenesis. In addition, the data show that the actions of ANXA1 in the inflammatory and neoplastic processes of the esophagus and stomach are specifically mediated by the FPR1 receptor.Padre Albino Integrated College (FIPA) Department of Physical and Biological Sciences, CatanduvaSão Paulo State University (UNESP) Department of Biology Laboratory of Immunomorphology, São José do Rio PretoSão Paulo State University (UNESP) Department of Biology Laboratory of Immunomorphology, São José do Rio PretoPadre Albino Integrated College (FIPA)Universidade Estadual Paulista (Unesp)Takaoka, Rodolfo T.C.Sertório, Nathália D.Magalini, Lara P.J.Dos Santos, Leandro M.Souza, Helena R.Iyomasa-Pilon, Melina M.Possebon, Lucas [UNESP]Costa, Sara S.Girol, Ana P. [UNESP]2018-12-11T17:17:06Z2018-12-11T17:17:06Z2018-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article181-186application/pdfhttp://dx.doi.org/10.1016/j.prp.2017.12.003Pathology Research and Practice, v. 214, n. 2, p. 181-186, 2018.1618-06310344-0338http://hdl.handle.net/11449/17569410.1016/j.prp.2017.12.0032-s2.0-850394527092-s2.0-85039452709.pdf2-s2.0-85039452709.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPathology Research and Practice0,547info:eu-repo/semantics/openAccess2023-10-23T06:11:55Zoai:repositorio.unesp.br:11449/175694Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:46:36.678457Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Expression profiles of Annexin A1, formylated peptide receptors and cyclooxigenase-2 in gastroesophageal inflammations and neoplasias |
title |
Expression profiles of Annexin A1, formylated peptide receptors and cyclooxigenase-2 in gastroesophageal inflammations and neoplasias |
spellingShingle |
Expression profiles of Annexin A1, formylated peptide receptors and cyclooxigenase-2 in gastroesophageal inflammations and neoplasias Takaoka, Rodolfo T.C. ANXA1 FPR Inflammation Tumors Upper digestive tract |
title_short |
Expression profiles of Annexin A1, formylated peptide receptors and cyclooxigenase-2 in gastroesophageal inflammations and neoplasias |
title_full |
Expression profiles of Annexin A1, formylated peptide receptors and cyclooxigenase-2 in gastroesophageal inflammations and neoplasias |
title_fullStr |
Expression profiles of Annexin A1, formylated peptide receptors and cyclooxigenase-2 in gastroesophageal inflammations and neoplasias |
title_full_unstemmed |
Expression profiles of Annexin A1, formylated peptide receptors and cyclooxigenase-2 in gastroesophageal inflammations and neoplasias |
title_sort |
Expression profiles of Annexin A1, formylated peptide receptors and cyclooxigenase-2 in gastroesophageal inflammations and neoplasias |
author |
Takaoka, Rodolfo T.C. |
author_facet |
Takaoka, Rodolfo T.C. Sertório, Nathália D. Magalini, Lara P.J. Dos Santos, Leandro M. Souza, Helena R. Iyomasa-Pilon, Melina M. Possebon, Lucas [UNESP] Costa, Sara S. Girol, Ana P. [UNESP] |
author_role |
author |
author2 |
Sertório, Nathália D. Magalini, Lara P.J. Dos Santos, Leandro M. Souza, Helena R. Iyomasa-Pilon, Melina M. Possebon, Lucas [UNESP] Costa, Sara S. Girol, Ana P. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Padre Albino Integrated College (FIPA) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Takaoka, Rodolfo T.C. Sertório, Nathália D. Magalini, Lara P.J. Dos Santos, Leandro M. Souza, Helena R. Iyomasa-Pilon, Melina M. Possebon, Lucas [UNESP] Costa, Sara S. Girol, Ana P. [UNESP] |
dc.subject.por.fl_str_mv |
ANXA1 FPR Inflammation Tumors Upper digestive tract |
topic |
ANXA1 FPR Inflammation Tumors Upper digestive tract |
description |
The anti−inflammatory protein Annexin−A1 (ANXA1) is associated to tumor invasion process and its actions can be mediated by formylated peptides receptors (FPRs). Therefore, we evaluated the expression and correlation of ANXA1, FPR and cyclooxygenase−2 (COX−2) enzyme in esophageal and stomach inflammations and neoplasias. The study of proteins was performed by immunohistochemistry in biopsies of esophagitis, Barrett's esophagus, squamous cell carcinoma and adenocarcinoma of the esophagus, as well as gastritis, stomach polypus and gastric adenocarcinoma. The intensity of the expressions was evaluated by densitometry. The immunohistochemical and densitometric analyzes showed specificity for the FPR1 receptor and modulation of the ANXA1, COX−2 and FPR1 expressions in the epithelial cells in the different studied conditions. Increased immunoreactivity of these proteins was observed in cases of inflammation and stomach polypus. Interestingly, moderate immunoreactivity for ANXA1 and FPR1 but increased immunolabeling for COX−2 were observed in Barrett́s esophagus and esophageal adenocarcinomas. Also, there was reduced expression of ANXA1 and FPR1 in esophageal carcinoma but COX−2 overexpression in this tumor. There was no expression of FPR2 but ANXA1 and FPR1 expressions were positively correlated in all clinical conditions studied. Positive correlation between ANXA1 and COX−2 were also observed in inflammation conditions while negative correlation between ANXA1 and COX−2 was observed in esophageal carcinoma. Our results demonstrate the unregulated expression of ANXA1 and COX−2 in precursor lesions of esophageal and stomach cancers, reinforcing their involvement in gastroesophageal carcinogenesis. In addition, the data show that the actions of ANXA1 in the inflammatory and neoplastic processes of the esophagus and stomach are specifically mediated by the FPR1 receptor. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:17:06Z 2018-12-11T17:17:06Z 2018-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.prp.2017.12.003 Pathology Research and Practice, v. 214, n. 2, p. 181-186, 2018. 1618-0631 0344-0338 http://hdl.handle.net/11449/175694 10.1016/j.prp.2017.12.003 2-s2.0-85039452709 2-s2.0-85039452709.pdf 2-s2.0-85039452709.pdf |
url |
http://dx.doi.org/10.1016/j.prp.2017.12.003 http://hdl.handle.net/11449/175694 |
identifier_str_mv |
Pathology Research and Practice, v. 214, n. 2, p. 181-186, 2018. 1618-0631 0344-0338 10.1016/j.prp.2017.12.003 2-s2.0-85039452709 2-s2.0-85039452709.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pathology Research and Practice 0,547 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
181-186 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128560608575488 |