Cytochemistry and polarization microscopy of amphibian sperm cell nuclei presumed to differ in basic protein composition
Autor(a) principal: | |
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Data de Publicação: | 1996 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1267/ahc.29.129 http://hdl.handle.net/11449/34578 |
Resumo: | The pattern of availability of free DNA phosphates, and the kind of DNA-protein complex arrangement, both induced by nuclear basic proteins, and the richness in arginine residues in these proteins were investigated cytochemically and cytophysically in spermatozoa of the South-American Hylidae species, Hyla fuscovaria and Hyla biobeba. The aim was to demonstrate differences at the level of sperm histones in two species of Hyla until recently considered to be congeneric. The results indicated differences in the spermatozoal nuclear basic proteins and DNA-protein complexes when the two species were compared. The spermatozoa of Hyla biobeba were assumed to be likely to contain a Bloch's ''type 3'' protein type (intermediate sperm basic protein), similarly to Hyla species of North and Central America. on the other hand, the data obtained for the spermatozoa of Hyla fuscovaria indicated that they contain a protamine or protamine-like protein, differing from Hyla biobeba and Hyla species of North and Central America. It is suggested that the differences reported here may be genus-specific, since Hyla fuscovaria has recently been reclassified as Scinax fuscovaria based on parameters other than sperm histone types. These findings are in agreement with the general view of a wide variability in sperm nuclear proteins in the Anura group. |
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Cytochemistry and polarization microscopy of amphibian sperm cell nuclei presumed to differ in basic protein compositionspermatozoanuclear proteinsAmphibiaCytochemistrypolarization microscopyThe pattern of availability of free DNA phosphates, and the kind of DNA-protein complex arrangement, both induced by nuclear basic proteins, and the richness in arginine residues in these proteins were investigated cytochemically and cytophysically in spermatozoa of the South-American Hylidae species, Hyla fuscovaria and Hyla biobeba. The aim was to demonstrate differences at the level of sperm histones in two species of Hyla until recently considered to be congeneric. The results indicated differences in the spermatozoal nuclear basic proteins and DNA-protein complexes when the two species were compared. The spermatozoa of Hyla biobeba were assumed to be likely to contain a Bloch's ''type 3'' protein type (intermediate sperm basic protein), similarly to Hyla species of North and Central America. on the other hand, the data obtained for the spermatozoa of Hyla fuscovaria indicated that they contain a protamine or protamine-like protein, differing from Hyla biobeba and Hyla species of North and Central America. It is suggested that the differences reported here may be genus-specific, since Hyla fuscovaria has recently been reclassified as Scinax fuscovaria based on parameters other than sperm histone types. These findings are in agreement with the general view of a wide variability in sperm nuclear proteins in the Anura group.UNICAMP,DEPT CELL BIOL,INST BIOL,BR-13083970 CAMPINAS,SP,BRAZILUNESP,IBILCE,DEPT BIOL,BR-15054000 S JOSE RIO PR,SP,BRAZILUNESP,IBILCE,DEPT BIOL,BR-15054000 S JOSE RIO PR,SP,BRAZILJapan Soc Histochem CytochemUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Taboga, S. R. [UNESP]Mello, M. L. S.Falco, J. R. P.Vidal, B. C.2014-05-20T15:23:53Z2014-05-20T15:23:53Z1996-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article129-134application/pdfhttp://dx.doi.org/10.1267/ahc.29.129Acta Histochemica Et Cytochemica. Kyoto: Japan Soc Histochem Cytochem, v. 29, n. 2, p. 129-134, 1996.0044-5991http://hdl.handle.net/11449/3457810.1267/ahc.29.129WOS:A1996VP965000072-s2.0-0030463059WOSA1996VP96500007.pdf14452594685261880000-0002-0970-4288Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Histochemica et Cytochemica0.8720,446info:eu-repo/semantics/openAccess2023-12-29T06:20:09Zoai:repositorio.unesp.br:11449/34578Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:36:18.153902Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytochemistry and polarization microscopy of amphibian sperm cell nuclei presumed to differ in basic protein composition |
title |
Cytochemistry and polarization microscopy of amphibian sperm cell nuclei presumed to differ in basic protein composition |
spellingShingle |
Cytochemistry and polarization microscopy of amphibian sperm cell nuclei presumed to differ in basic protein composition Taboga, S. R. [UNESP] spermatozoa nuclear proteins Amphibia Cytochemistry polarization microscopy |
title_short |
Cytochemistry and polarization microscopy of amphibian sperm cell nuclei presumed to differ in basic protein composition |
title_full |
Cytochemistry and polarization microscopy of amphibian sperm cell nuclei presumed to differ in basic protein composition |
title_fullStr |
Cytochemistry and polarization microscopy of amphibian sperm cell nuclei presumed to differ in basic protein composition |
title_full_unstemmed |
Cytochemistry and polarization microscopy of amphibian sperm cell nuclei presumed to differ in basic protein composition |
title_sort |
Cytochemistry and polarization microscopy of amphibian sperm cell nuclei presumed to differ in basic protein composition |
author |
Taboga, S. R. [UNESP] |
author_facet |
Taboga, S. R. [UNESP] Mello, M. L. S. Falco, J. R. P. Vidal, B. C. |
author_role |
author |
author2 |
Mello, M. L. S. Falco, J. R. P. Vidal, B. C. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Taboga, S. R. [UNESP] Mello, M. L. S. Falco, J. R. P. Vidal, B. C. |
dc.subject.por.fl_str_mv |
spermatozoa nuclear proteins Amphibia Cytochemistry polarization microscopy |
topic |
spermatozoa nuclear proteins Amphibia Cytochemistry polarization microscopy |
description |
The pattern of availability of free DNA phosphates, and the kind of DNA-protein complex arrangement, both induced by nuclear basic proteins, and the richness in arginine residues in these proteins were investigated cytochemically and cytophysically in spermatozoa of the South-American Hylidae species, Hyla fuscovaria and Hyla biobeba. The aim was to demonstrate differences at the level of sperm histones in two species of Hyla until recently considered to be congeneric. The results indicated differences in the spermatozoal nuclear basic proteins and DNA-protein complexes when the two species were compared. The spermatozoa of Hyla biobeba were assumed to be likely to contain a Bloch's ''type 3'' protein type (intermediate sperm basic protein), similarly to Hyla species of North and Central America. on the other hand, the data obtained for the spermatozoa of Hyla fuscovaria indicated that they contain a protamine or protamine-like protein, differing from Hyla biobeba and Hyla species of North and Central America. It is suggested that the differences reported here may be genus-specific, since Hyla fuscovaria has recently been reclassified as Scinax fuscovaria based on parameters other than sperm histone types. These findings are in agreement with the general view of a wide variability in sperm nuclear proteins in the Anura group. |
publishDate |
1996 |
dc.date.none.fl_str_mv |
1996-01-01 2014-05-20T15:23:53Z 2014-05-20T15:23:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1267/ahc.29.129 Acta Histochemica Et Cytochemica. Kyoto: Japan Soc Histochem Cytochem, v. 29, n. 2, p. 129-134, 1996. 0044-5991 http://hdl.handle.net/11449/34578 10.1267/ahc.29.129 WOS:A1996VP96500007 2-s2.0-0030463059 WOSA1996VP96500007.pdf 1445259468526188 0000-0002-0970-4288 |
url |
http://dx.doi.org/10.1267/ahc.29.129 http://hdl.handle.net/11449/34578 |
identifier_str_mv |
Acta Histochemica Et Cytochemica. Kyoto: Japan Soc Histochem Cytochem, v. 29, n. 2, p. 129-134, 1996. 0044-5991 10.1267/ahc.29.129 WOS:A1996VP96500007 2-s2.0-0030463059 WOSA1996VP96500007.pdf 1445259468526188 0000-0002-0970-4288 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Histochemica et Cytochemica 0.872 0,446 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
129-134 application/pdf |
dc.publisher.none.fl_str_mv |
Japan Soc Histochem Cytochem |
publisher.none.fl_str_mv |
Japan Soc Histochem Cytochem |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129340787916800 |