A Comparative Genotoxicity Study of a Supraphysiological Dose of Triiodothyronine (T3) in Obese Rats Subjected to Either Calorie-Restricted Diet or Hyperthyroidism

Detalhes bibliográficos
Autor(a) principal: de Sibio, Maria Teresa [UNESP]
Data de Publicação: 2013
Outros Autores: Luvizotto, Renata Azevedo Melo [UNESP], Olimpio, Regiane Marques Castro [UNESP], Corrêa, Camila Renata [UNESP], Marino, Juliana [UNESP], de Oliveira, Miriane [UNESP], Conde, Sandro José [UNESP], Ferreira, Ana Lúcia dos Anjos [UNESP], Padovani, Carlos Roberto [UNESP], Nogueira, Célia Regina [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1371/journal.pone.0056913
http://hdl.handle.net/11449/74638
Resumo: This study was designed to determine the genotoxicity of a supraphysiological dose of triiodothyronine (T3) in both obese and calorie-restricted obese animals. Fifty male Wistar rats were randomly assigned to one of the two following groups: control (C; n = 10) and obese (OB; n = 40). The C group received standard food, whereas the OB group was fed a hypercaloric diet for 20 weeks. After this period, half of the OB animals (n = 20) were subjected to a 25%-calorie restriction of standard diet for 8 weeks forming thus a new group (OR), whereas the remaining OB animals were kept on the initial hypercaloric diet. During the following two weeks, 10 OR animals continued on the calorie restriction diet, whereas the remaining 10 rats of this group formed a new group (ORS) given a supraphysiological dose of T3 (25 μg/100 g body weight) along with the calorie restriction diet. Similarly, the remaining OB animals were divided into two groups, one that continued on the hypercaloric diet (OB, n = 10), and one that received the supraphysiological dose of T3 (25 μg/100 g body weight) along with the hypercaloric diet (OS, n = 10) for two weeks. The OB group showed weight gain, increased adiposity, insulin resistance, increased leptin levels and genotoxicity; T3 administration in OS animals led to an increase in genotoxicity and oxidative stress when compared with the OB group. The OR group showed weight loss and normalized levels of adiposity, insulin resistance, serum leptin and genotoxicity, thus having features similar to those of the C group. On the other hand, the ORS group, compared to OR animals, showed higher genotoxicity. Our results indicate that regardless of diet, a supraphysiological dose of T3 causes genotoxicity and potentiates oxidative stress. © 2013 de Sibio et al.
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spelling A Comparative Genotoxicity Study of a Supraphysiological Dose of Triiodothyronine (T3) in Obese Rats Subjected to Either Calorie-Restricted Diet or Hyperthyroidismleptinliothyroninemalonaldehydeanimal cellanimal experimentanimal modelcaloric intakecaloric restrictioncomet assaycontrolled studyDNA damagegenotoxicityinsulin resistancelipid storagemalenonhumanobesityoxidative stressprotein blood levelratweight gainweight reductionAdipose TissueAnimalsBody CompositionBody WeightCaloric RestrictionComet AssayEnergy IntakeHyperthyroidismInsulin ResistanceLeptinMaleMalondialdehydeObesityRatsTriiodothyronineThis study was designed to determine the genotoxicity of a supraphysiological dose of triiodothyronine (T3) in both obese and calorie-restricted obese animals. Fifty male Wistar rats were randomly assigned to one of the two following groups: control (C; n = 10) and obese (OB; n = 40). The C group received standard food, whereas the OB group was fed a hypercaloric diet for 20 weeks. After this period, half of the OB animals (n = 20) were subjected to a 25%-calorie restriction of standard diet for 8 weeks forming thus a new group (OR), whereas the remaining OB animals were kept on the initial hypercaloric diet. During the following two weeks, 10 OR animals continued on the calorie restriction diet, whereas the remaining 10 rats of this group formed a new group (ORS) given a supraphysiological dose of T3 (25 μg/100 g body weight) along with the calorie restriction diet. Similarly, the remaining OB animals were divided into two groups, one that continued on the hypercaloric diet (OB, n = 10), and one that received the supraphysiological dose of T3 (25 μg/100 g body weight) along with the hypercaloric diet (OS, n = 10) for two weeks. The OB group showed weight gain, increased adiposity, insulin resistance, increased leptin levels and genotoxicity; T3 administration in OS animals led to an increase in genotoxicity and oxidative stress when compared with the OB group. The OR group showed weight loss and normalized levels of adiposity, insulin resistance, serum leptin and genotoxicity, thus having features similar to those of the C group. On the other hand, the ORS group, compared to OR animals, showed higher genotoxicity. Our results indicate that regardless of diet, a supraphysiological dose of T3 causes genotoxicity and potentiates oxidative stress. © 2013 de Sibio et al.Department of Internal Medicine Botucatu Medical School - University of Sao Paulo State (UNESP), Botucatu, SPDepartment of Pathology Botucatu Medical School - University of Sao Paulo State (UNESP), Botucatu, SPDepartment of Biostatistics Biosciences Institute - University of Sao Paulo State (UNESP), Botucatu, SPDepartment of Internal Medicine Botucatu Medical School - University of Sao Paulo State (UNESP), Botucatu, SPDepartment of Pathology Botucatu Medical School - University of Sao Paulo State (UNESP), Botucatu, SPDepartment of Biostatistics Biosciences Institute - University of Sao Paulo State (UNESP), Botucatu, SPUniversidade Estadual Paulista (Unesp)de Sibio, Maria Teresa [UNESP]Luvizotto, Renata Azevedo Melo [UNESP]Olimpio, Regiane Marques Castro [UNESP]Corrêa, Camila Renata [UNESP]Marino, Juliana [UNESP]de Oliveira, Miriane [UNESP]Conde, Sandro José [UNESP]Ferreira, Ana Lúcia dos Anjos [UNESP]Padovani, Carlos Roberto [UNESP]Nogueira, Célia Regina [UNESP]2014-05-27T11:28:33Z2014-05-27T11:28:33Z2013-02-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0056913PLoS ONE, v. 8, n. 2, 2013.1932-6203http://hdl.handle.net/11449/7463810.1371/journal.pone.0056913WOS:0003155249000432-s2.0-848745115182-s2.0-84874511518.pdf87278970805222892940051650846541Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLOS ONE2.7661,164info:eu-repo/semantics/openAccess2024-09-03T13:17:56Zoai:repositorio.unesp.br:11449/74638Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:17:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A Comparative Genotoxicity Study of a Supraphysiological Dose of Triiodothyronine (T3) in Obese Rats Subjected to Either Calorie-Restricted Diet or Hyperthyroidism
title A Comparative Genotoxicity Study of a Supraphysiological Dose of Triiodothyronine (T3) in Obese Rats Subjected to Either Calorie-Restricted Diet or Hyperthyroidism
spellingShingle A Comparative Genotoxicity Study of a Supraphysiological Dose of Triiodothyronine (T3) in Obese Rats Subjected to Either Calorie-Restricted Diet or Hyperthyroidism
de Sibio, Maria Teresa [UNESP]
leptin
liothyronine
malonaldehyde
animal cell
animal experiment
animal model
caloric intake
caloric restriction
comet assay
controlled study
DNA damage
genotoxicity
insulin resistance
lipid storage
male
nonhuman
obesity
oxidative stress
protein blood level
rat
weight gain
weight reduction
Adipose Tissue
Animals
Body Composition
Body Weight
Caloric Restriction
Comet Assay
Energy Intake
Hyperthyroidism
Insulin Resistance
Leptin
Male
Malondialdehyde
Obesity
Rats
Triiodothyronine
title_short A Comparative Genotoxicity Study of a Supraphysiological Dose of Triiodothyronine (T3) in Obese Rats Subjected to Either Calorie-Restricted Diet or Hyperthyroidism
title_full A Comparative Genotoxicity Study of a Supraphysiological Dose of Triiodothyronine (T3) in Obese Rats Subjected to Either Calorie-Restricted Diet or Hyperthyroidism
title_fullStr A Comparative Genotoxicity Study of a Supraphysiological Dose of Triiodothyronine (T3) in Obese Rats Subjected to Either Calorie-Restricted Diet or Hyperthyroidism
title_full_unstemmed A Comparative Genotoxicity Study of a Supraphysiological Dose of Triiodothyronine (T3) in Obese Rats Subjected to Either Calorie-Restricted Diet or Hyperthyroidism
title_sort A Comparative Genotoxicity Study of a Supraphysiological Dose of Triiodothyronine (T3) in Obese Rats Subjected to Either Calorie-Restricted Diet or Hyperthyroidism
author de Sibio, Maria Teresa [UNESP]
author_facet de Sibio, Maria Teresa [UNESP]
Luvizotto, Renata Azevedo Melo [UNESP]
Olimpio, Regiane Marques Castro [UNESP]
Corrêa, Camila Renata [UNESP]
Marino, Juliana [UNESP]
de Oliveira, Miriane [UNESP]
Conde, Sandro José [UNESP]
Ferreira, Ana Lúcia dos Anjos [UNESP]
Padovani, Carlos Roberto [UNESP]
Nogueira, Célia Regina [UNESP]
author_role author
author2 Luvizotto, Renata Azevedo Melo [UNESP]
Olimpio, Regiane Marques Castro [UNESP]
Corrêa, Camila Renata [UNESP]
Marino, Juliana [UNESP]
de Oliveira, Miriane [UNESP]
Conde, Sandro José [UNESP]
Ferreira, Ana Lúcia dos Anjos [UNESP]
Padovani, Carlos Roberto [UNESP]
Nogueira, Célia Regina [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv de Sibio, Maria Teresa [UNESP]
Luvizotto, Renata Azevedo Melo [UNESP]
Olimpio, Regiane Marques Castro [UNESP]
Corrêa, Camila Renata [UNESP]
Marino, Juliana [UNESP]
de Oliveira, Miriane [UNESP]
Conde, Sandro José [UNESP]
Ferreira, Ana Lúcia dos Anjos [UNESP]
Padovani, Carlos Roberto [UNESP]
Nogueira, Célia Regina [UNESP]
dc.subject.por.fl_str_mv leptin
liothyronine
malonaldehyde
animal cell
animal experiment
animal model
caloric intake
caloric restriction
comet assay
controlled study
DNA damage
genotoxicity
insulin resistance
lipid storage
male
nonhuman
obesity
oxidative stress
protein blood level
rat
weight gain
weight reduction
Adipose Tissue
Animals
Body Composition
Body Weight
Caloric Restriction
Comet Assay
Energy Intake
Hyperthyroidism
Insulin Resistance
Leptin
Male
Malondialdehyde
Obesity
Rats
Triiodothyronine
topic leptin
liothyronine
malonaldehyde
animal cell
animal experiment
animal model
caloric intake
caloric restriction
comet assay
controlled study
DNA damage
genotoxicity
insulin resistance
lipid storage
male
nonhuman
obesity
oxidative stress
protein blood level
rat
weight gain
weight reduction
Adipose Tissue
Animals
Body Composition
Body Weight
Caloric Restriction
Comet Assay
Energy Intake
Hyperthyroidism
Insulin Resistance
Leptin
Male
Malondialdehyde
Obesity
Rats
Triiodothyronine
description This study was designed to determine the genotoxicity of a supraphysiological dose of triiodothyronine (T3) in both obese and calorie-restricted obese animals. Fifty male Wistar rats were randomly assigned to one of the two following groups: control (C; n = 10) and obese (OB; n = 40). The C group received standard food, whereas the OB group was fed a hypercaloric diet for 20 weeks. After this period, half of the OB animals (n = 20) were subjected to a 25%-calorie restriction of standard diet for 8 weeks forming thus a new group (OR), whereas the remaining OB animals were kept on the initial hypercaloric diet. During the following two weeks, 10 OR animals continued on the calorie restriction diet, whereas the remaining 10 rats of this group formed a new group (ORS) given a supraphysiological dose of T3 (25 μg/100 g body weight) along with the calorie restriction diet. Similarly, the remaining OB animals were divided into two groups, one that continued on the hypercaloric diet (OB, n = 10), and one that received the supraphysiological dose of T3 (25 μg/100 g body weight) along with the hypercaloric diet (OS, n = 10) for two weeks. The OB group showed weight gain, increased adiposity, insulin resistance, increased leptin levels and genotoxicity; T3 administration in OS animals led to an increase in genotoxicity and oxidative stress when compared with the OB group. The OR group showed weight loss and normalized levels of adiposity, insulin resistance, serum leptin and genotoxicity, thus having features similar to those of the C group. On the other hand, the ORS group, compared to OR animals, showed higher genotoxicity. Our results indicate that regardless of diet, a supraphysiological dose of T3 causes genotoxicity and potentiates oxidative stress. © 2013 de Sibio et al.
publishDate 2013
dc.date.none.fl_str_mv 2013-02-28
2014-05-27T11:28:33Z
2014-05-27T11:28:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0056913
PLoS ONE, v. 8, n. 2, 2013.
1932-6203
http://hdl.handle.net/11449/74638
10.1371/journal.pone.0056913
WOS:000315524900043
2-s2.0-84874511518
2-s2.0-84874511518.pdf
8727897080522289
2940051650846541
url http://dx.doi.org/10.1371/journal.pone.0056913
http://hdl.handle.net/11449/74638
identifier_str_mv PLoS ONE, v. 8, n. 2, 2013.
1932-6203
10.1371/journal.pone.0056913
WOS:000315524900043
2-s2.0-84874511518
2-s2.0-84874511518.pdf
8727897080522289
2940051650846541
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PLOS ONE
2.766
1,164
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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