Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11010-015-2598-5 http://hdl.handle.net/11449/161199 |
Resumo: | The activated renin-angiotensin-aldosterone system modulates several metabolic pathways that contribute to left ventricular hypertrophy and heart failure. In this metabolic system, angiotensin II modulates heart morphophysiological changes triggered by a series of inflammatory and pro-inflammatory responses; however, the fine tuning associated with the control of this biochemical pathway remains unknown. Here, we investigated elements involved in the post-transcriptional regulation of the pro-inflammatory environment in the H9c2 cardiac cell line, focusing on miRNA elements that modulate PTEN expression. A cellular model of investigation was established and the miR-315-5p was identified as a novel element targeting PTEN in this cardiac cell line, thereby controlling the protein level. This interconnected pathway contributes to the control of the pro-inflammatory environment in Ang II-treated cells. |
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Repositório Institucional da UNESP |
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Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expressionAngiotensin IICell culturemiRNAPost-transcriptional regulationPTENThe activated renin-angiotensin-aldosterone system modulates several metabolic pathways that contribute to left ventricular hypertrophy and heart failure. In this metabolic system, angiotensin II modulates heart morphophysiological changes triggered by a series of inflammatory and pro-inflammatory responses; however, the fine tuning associated with the control of this biochemical pathway remains unknown. Here, we investigated elements involved in the post-transcriptional regulation of the pro-inflammatory environment in the H9c2 cardiac cell line, focusing on miRNA elements that modulate PTEN expression. A cellular model of investigation was established and the miR-315-5p was identified as a novel element targeting PTEN in this cardiac cell line, thereby controlling the protein level. This interconnected pathway contributes to the control of the pro-inflammatory environment in Ang II-treated cells.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)INCT-Nano-BiofarmaceuticaUniv Fed Ouro Preto, Nucleo Pesquisa Biol, Inst Ciencias Exatas & Biol, Lab Bioquim & Biol Mol, Ouro Preto, MG, BrazilUniv Fed Minas Gerais, Dept Fisiol & Biofis, Lab Fisiol, Belo Horizonte, MG, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Biol, Mol Biol Lab, BR-13506900 Rio Claro, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Biol, Mol Biol Lab, BR-13506900 Rio Claro, SP, BrazilFAPESP: FAPESP-2009/07671-2CNPq: 475586/2009-3SpringerUniv Fed Ouro PretoUniversidade Federal de Minas Gerais (UFMG)Universidade Estadual Paulista (Unesp)Silva, Walmir daSouza dos Santos, Robson AugustoMoraes, Karen C. M. [UNESP]2018-11-26T16:19:31Z2018-11-26T16:19:31Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article363-371application/pdfhttp://dx.doi.org/10.1007/s11010-015-2598-5Molecular And Cellular Biochemistry. Dordrecht: Springer, v. 411, n. 1-2, p. 363-371, 2016.0300-8177http://hdl.handle.net/11449/16119910.1007/s11010-015-2598-5WOS:000369808600036WOS000369808600036.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMolecular And Cellular Biochemistry1,003info:eu-repo/semantics/openAccess2023-12-26T06:21:41Zoai:repositorio.unesp.br:11449/161199Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:22:36.497159Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression |
title |
Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression |
spellingShingle |
Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression Silva, Walmir da Angiotensin II Cell culture miRNA Post-transcriptional regulation PTEN |
title_short |
Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression |
title_full |
Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression |
title_fullStr |
Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression |
title_full_unstemmed |
Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression |
title_sort |
Mir-351-5p contributes to the establishment of a pro-inflammatory environment in the H9c2 cell line by repressing PTEN expression |
author |
Silva, Walmir da |
author_facet |
Silva, Walmir da Souza dos Santos, Robson Augusto Moraes, Karen C. M. [UNESP] |
author_role |
author |
author2 |
Souza dos Santos, Robson Augusto Moraes, Karen C. M. [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Univ Fed Ouro Preto Universidade Federal de Minas Gerais (UFMG) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silva, Walmir da Souza dos Santos, Robson Augusto Moraes, Karen C. M. [UNESP] |
dc.subject.por.fl_str_mv |
Angiotensin II Cell culture miRNA Post-transcriptional regulation PTEN |
topic |
Angiotensin II Cell culture miRNA Post-transcriptional regulation PTEN |
description |
The activated renin-angiotensin-aldosterone system modulates several metabolic pathways that contribute to left ventricular hypertrophy and heart failure. In this metabolic system, angiotensin II modulates heart morphophysiological changes triggered by a series of inflammatory and pro-inflammatory responses; however, the fine tuning associated with the control of this biochemical pathway remains unknown. Here, we investigated elements involved in the post-transcriptional regulation of the pro-inflammatory environment in the H9c2 cardiac cell line, focusing on miRNA elements that modulate PTEN expression. A cellular model of investigation was established and the miR-315-5p was identified as a novel element targeting PTEN in this cardiac cell line, thereby controlling the protein level. This interconnected pathway contributes to the control of the pro-inflammatory environment in Ang II-treated cells. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-11-26T16:19:31Z 2018-11-26T16:19:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11010-015-2598-5 Molecular And Cellular Biochemistry. Dordrecht: Springer, v. 411, n. 1-2, p. 363-371, 2016. 0300-8177 http://hdl.handle.net/11449/161199 10.1007/s11010-015-2598-5 WOS:000369808600036 WOS000369808600036.pdf |
url |
http://dx.doi.org/10.1007/s11010-015-2598-5 http://hdl.handle.net/11449/161199 |
identifier_str_mv |
Molecular And Cellular Biochemistry. Dordrecht: Springer, v. 411, n. 1-2, p. 363-371, 2016. 0300-8177 10.1007/s11010-015-2598-5 WOS:000369808600036 WOS000369808600036.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecular And Cellular Biochemistry 1,003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
363-371 application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129314991898624 |