Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis

Detalhes bibliográficos
Autor(a) principal: dos Santos Alves, Taila
Data de Publicação: 2023
Outros Autores: Rosa, Vinícius Sanches, da Silva Leite, Domingos, Guerra, Simony Trevizan [UNESP], Joaquim, Sâmea Fernandes [UNESP], Guimarães, Felipe Freitas [UNESP], de Figueiredo Pantoja, José Carlos [UNESP], Lucheis, Simoni Baldini [UNESP], Rall, Vera Lúcia Mores [UNESP], Hernandes, Rodrigo Tavanelli [UNESP], Langoni, Helio [UNESP], Ribeiro, Márcio Garcia [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
DOI: 10.1007/s00284-023-03191-6
Texto Completo: http://dx.doi.org/10.1007/s00284-023-03191-6
http://hdl.handle.net/11449/248288
Resumo: Mastitis occurrence in dairy cows is a broad topic that involves several sectors, from antimicrobial resistance and virulence of strains to economic implications and cattle management practices. Here, we assessed the molecular characterization (antimicrobial resistance determinants, virulence genes, sequences type, serotypes, and plasmid types) of 178 Escherichia coli strains isolated from milk samples from cows with clinical mastitis using a genome-based k-mers approach. Of these, 53 (29.8%) showed multidrug resistance by disc diffusion. We selected eight multidrug-resistant mastitis-associated E. coli for whole-genome sequencing and molecular characterization based on raw data using k-mers. We assessed antimicrobial resistance genes, virulence factors, serotypes, Multilocus Sequence Typing (MLST), and plasmid types. The most antimicrobial resistance gene found were blaTEM-1B (7/8), tetA (6/8), strA (6/8), strB (6/8), and qnrB19 (5/8). A total of 25 virulence factors were detected encoding adhesins, capsule, enzymes/proteins, increased serum survival, hemolysin, colicins, and iron uptake. These virulence factors were associated with Extraintestinal Pathogenic E. coli. Three pandemic clones were found: ST10, ST101, and ST69. Two E. coli were assigned in the O117 serogroup and one in the O8:H25 serotype. The most common plasmid groups were IncFII (7/8) and IncFIB (6/8). Our findings contribute to the knowledge of virulence mechanisms, epidemiological aspects, and antimicrobial resistance determinants of E. coli strains obtained from clinical mammary infections of cows.
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spelling Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine MastitisMastitis occurrence in dairy cows is a broad topic that involves several sectors, from antimicrobial resistance and virulence of strains to economic implications and cattle management practices. Here, we assessed the molecular characterization (antimicrobial resistance determinants, virulence genes, sequences type, serotypes, and plasmid types) of 178 Escherichia coli strains isolated from milk samples from cows with clinical mastitis using a genome-based k-mers approach. Of these, 53 (29.8%) showed multidrug resistance by disc diffusion. We selected eight multidrug-resistant mastitis-associated E. coli for whole-genome sequencing and molecular characterization based on raw data using k-mers. We assessed antimicrobial resistance genes, virulence factors, serotypes, Multilocus Sequence Typing (MLST), and plasmid types. The most antimicrobial resistance gene found were blaTEM-1B (7/8), tetA (6/8), strA (6/8), strB (6/8), and qnrB19 (5/8). A total of 25 virulence factors were detected encoding adhesins, capsule, enzymes/proteins, increased serum survival, hemolysin, colicins, and iron uptake. These virulence factors were associated with Extraintestinal Pathogenic E. coli. Three pandemic clones were found: ST10, ST101, and ST69. Two E. coli were assigned in the O117 serogroup and one in the O8:H25 serotype. The most common plasmid groups were IncFII (7/8) and IncFIB (6/8). Our findings contribute to the knowledge of virulence mechanisms, epidemiological aspects, and antimicrobial resistance determinants of E. coli strains obtained from clinical mammary infections of cows.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Genetics Evolution Microbiology and Immunology Institute of Biology University of Campinas-UNICAMP, SPDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Sciences São Paulo State University-UNESP, SPDepartment of Microbiology and Immunology São Paulo State University-UNESP, SPDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Sciences São Paulo State University-UNESP, SPDepartment of Microbiology and Immunology São Paulo State University-UNESP, SPFAPESP: 2015/19688-8Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)dos Santos Alves, TailaRosa, Vinícius Sanchesda Silva Leite, DomingosGuerra, Simony Trevizan [UNESP]Joaquim, Sâmea Fernandes [UNESP]Guimarães, Felipe Freitas [UNESP]de Figueiredo Pantoja, José Carlos [UNESP]Lucheis, Simoni Baldini [UNESP]Rall, Vera Lúcia Mores [UNESP]Hernandes, Rodrigo Tavanelli [UNESP]Langoni, Helio [UNESP]Ribeiro, Márcio Garcia [UNESP]2023-07-29T13:39:41Z2023-07-29T13:39:41Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00284-023-03191-6Current Microbiology, v. 80, n. 3, 2023.1432-09910343-8651http://hdl.handle.net/11449/24828810.1007/s00284-023-03191-62-s2.0-85147178135Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCurrent Microbiologyinfo:eu-repo/semantics/openAccess2024-09-09T13:00:44Zoai:repositorio.unesp.br:11449/248288Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T13:00:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis
title Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis
spellingShingle Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis
Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis
dos Santos Alves, Taila
dos Santos Alves, Taila
title_short Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis
title_full Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis
title_fullStr Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis
Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis
title_full_unstemmed Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis
Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis
title_sort Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis
author dos Santos Alves, Taila
author_facet dos Santos Alves, Taila
dos Santos Alves, Taila
Rosa, Vinícius Sanches
da Silva Leite, Domingos
Guerra, Simony Trevizan [UNESP]
Joaquim, Sâmea Fernandes [UNESP]
Guimarães, Felipe Freitas [UNESP]
de Figueiredo Pantoja, José Carlos [UNESP]
Lucheis, Simoni Baldini [UNESP]
Rall, Vera Lúcia Mores [UNESP]
Hernandes, Rodrigo Tavanelli [UNESP]
Langoni, Helio [UNESP]
Ribeiro, Márcio Garcia [UNESP]
Rosa, Vinícius Sanches
da Silva Leite, Domingos
Guerra, Simony Trevizan [UNESP]
Joaquim, Sâmea Fernandes [UNESP]
Guimarães, Felipe Freitas [UNESP]
de Figueiredo Pantoja, José Carlos [UNESP]
Lucheis, Simoni Baldini [UNESP]
Rall, Vera Lúcia Mores [UNESP]
Hernandes, Rodrigo Tavanelli [UNESP]
Langoni, Helio [UNESP]
Ribeiro, Márcio Garcia [UNESP]
author_role author
author2 Rosa, Vinícius Sanches
da Silva Leite, Domingos
Guerra, Simony Trevizan [UNESP]
Joaquim, Sâmea Fernandes [UNESP]
Guimarães, Felipe Freitas [UNESP]
de Figueiredo Pantoja, José Carlos [UNESP]
Lucheis, Simoni Baldini [UNESP]
Rall, Vera Lúcia Mores [UNESP]
Hernandes, Rodrigo Tavanelli [UNESP]
Langoni, Helio [UNESP]
Ribeiro, Márcio Garcia [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv dos Santos Alves, Taila
Rosa, Vinícius Sanches
da Silva Leite, Domingos
Guerra, Simony Trevizan [UNESP]
Joaquim, Sâmea Fernandes [UNESP]
Guimarães, Felipe Freitas [UNESP]
de Figueiredo Pantoja, José Carlos [UNESP]
Lucheis, Simoni Baldini [UNESP]
Rall, Vera Lúcia Mores [UNESP]
Hernandes, Rodrigo Tavanelli [UNESP]
Langoni, Helio [UNESP]
Ribeiro, Márcio Garcia [UNESP]
description Mastitis occurrence in dairy cows is a broad topic that involves several sectors, from antimicrobial resistance and virulence of strains to economic implications and cattle management practices. Here, we assessed the molecular characterization (antimicrobial resistance determinants, virulence genes, sequences type, serotypes, and plasmid types) of 178 Escherichia coli strains isolated from milk samples from cows with clinical mastitis using a genome-based k-mers approach. Of these, 53 (29.8%) showed multidrug resistance by disc diffusion. We selected eight multidrug-resistant mastitis-associated E. coli for whole-genome sequencing and molecular characterization based on raw data using k-mers. We assessed antimicrobial resistance genes, virulence factors, serotypes, Multilocus Sequence Typing (MLST), and plasmid types. The most antimicrobial resistance gene found were blaTEM-1B (7/8), tetA (6/8), strA (6/8), strB (6/8), and qnrB19 (5/8). A total of 25 virulence factors were detected encoding adhesins, capsule, enzymes/proteins, increased serum survival, hemolysin, colicins, and iron uptake. These virulence factors were associated with Extraintestinal Pathogenic E. coli. Three pandemic clones were found: ST10, ST101, and ST69. Two E. coli were assigned in the O117 serogroup and one in the O8:H25 serotype. The most common plasmid groups were IncFII (7/8) and IncFIB (6/8). Our findings contribute to the knowledge of virulence mechanisms, epidemiological aspects, and antimicrobial resistance determinants of E. coli strains obtained from clinical mammary infections of cows.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:39:41Z
2023-07-29T13:39:41Z
2023-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00284-023-03191-6
Current Microbiology, v. 80, n. 3, 2023.
1432-0991
0343-8651
http://hdl.handle.net/11449/248288
10.1007/s00284-023-03191-6
2-s2.0-85147178135
url http://dx.doi.org/10.1007/s00284-023-03191-6
http://hdl.handle.net/11449/248288
identifier_str_mv Current Microbiology, v. 80, n. 3, 2023.
1432-0991
0343-8651
10.1007/s00284-023-03191-6
2-s2.0-85147178135
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Current Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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dc.identifier.doi.none.fl_str_mv 10.1007/s00284-023-03191-6