Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
DOI: | 10.1007/s00284-023-03191-6 |
Texto Completo: | http://dx.doi.org/10.1007/s00284-023-03191-6 http://hdl.handle.net/11449/248288 |
Resumo: | Mastitis occurrence in dairy cows is a broad topic that involves several sectors, from antimicrobial resistance and virulence of strains to economic implications and cattle management practices. Here, we assessed the molecular characterization (antimicrobial resistance determinants, virulence genes, sequences type, serotypes, and plasmid types) of 178 Escherichia coli strains isolated from milk samples from cows with clinical mastitis using a genome-based k-mers approach. Of these, 53 (29.8%) showed multidrug resistance by disc diffusion. We selected eight multidrug-resistant mastitis-associated E. coli for whole-genome sequencing and molecular characterization based on raw data using k-mers. We assessed antimicrobial resistance genes, virulence factors, serotypes, Multilocus Sequence Typing (MLST), and plasmid types. The most antimicrobial resistance gene found were blaTEM-1B (7/8), tetA (6/8), strA (6/8), strB (6/8), and qnrB19 (5/8). A total of 25 virulence factors were detected encoding adhesins, capsule, enzymes/proteins, increased serum survival, hemolysin, colicins, and iron uptake. These virulence factors were associated with Extraintestinal Pathogenic E. coli. Three pandemic clones were found: ST10, ST101, and ST69. Two E. coli were assigned in the O117 serogroup and one in the O8:H25 serotype. The most common plasmid groups were IncFII (7/8) and IncFIB (6/8). Our findings contribute to the knowledge of virulence mechanisms, epidemiological aspects, and antimicrobial resistance determinants of E. coli strains obtained from clinical mammary infections of cows. |
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Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine MastitisMastitis occurrence in dairy cows is a broad topic that involves several sectors, from antimicrobial resistance and virulence of strains to economic implications and cattle management practices. Here, we assessed the molecular characterization (antimicrobial resistance determinants, virulence genes, sequences type, serotypes, and plasmid types) of 178 Escherichia coli strains isolated from milk samples from cows with clinical mastitis using a genome-based k-mers approach. Of these, 53 (29.8%) showed multidrug resistance by disc diffusion. We selected eight multidrug-resistant mastitis-associated E. coli for whole-genome sequencing and molecular characterization based on raw data using k-mers. We assessed antimicrobial resistance genes, virulence factors, serotypes, Multilocus Sequence Typing (MLST), and plasmid types. The most antimicrobial resistance gene found were blaTEM-1B (7/8), tetA (6/8), strA (6/8), strB (6/8), and qnrB19 (5/8). A total of 25 virulence factors were detected encoding adhesins, capsule, enzymes/proteins, increased serum survival, hemolysin, colicins, and iron uptake. These virulence factors were associated with Extraintestinal Pathogenic E. coli. Three pandemic clones were found: ST10, ST101, and ST69. Two E. coli were assigned in the O117 serogroup and one in the O8:H25 serotype. The most common plasmid groups were IncFII (7/8) and IncFIB (6/8). Our findings contribute to the knowledge of virulence mechanisms, epidemiological aspects, and antimicrobial resistance determinants of E. coli strains obtained from clinical mammary infections of cows.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Genetics Evolution Microbiology and Immunology Institute of Biology University of Campinas-UNICAMP, SPDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Sciences São Paulo State University-UNESP, SPDepartment of Microbiology and Immunology São Paulo State University-UNESP, SPDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Sciences São Paulo State University-UNESP, SPDepartment of Microbiology and Immunology São Paulo State University-UNESP, SPFAPESP: 2015/19688-8Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)dos Santos Alves, TailaRosa, Vinícius Sanchesda Silva Leite, DomingosGuerra, Simony Trevizan [UNESP]Joaquim, Sâmea Fernandes [UNESP]Guimarães, Felipe Freitas [UNESP]de Figueiredo Pantoja, José Carlos [UNESP]Lucheis, Simoni Baldini [UNESP]Rall, Vera Lúcia Mores [UNESP]Hernandes, Rodrigo Tavanelli [UNESP]Langoni, Helio [UNESP]Ribeiro, Márcio Garcia [UNESP]2023-07-29T13:39:41Z2023-07-29T13:39:41Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00284-023-03191-6Current Microbiology, v. 80, n. 3, 2023.1432-09910343-8651http://hdl.handle.net/11449/24828810.1007/s00284-023-03191-62-s2.0-85147178135Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCurrent Microbiologyinfo:eu-repo/semantics/openAccess2024-09-09T13:00:44Zoai:repositorio.unesp.br:11449/248288Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T13:00:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis |
title |
Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis |
spellingShingle |
Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis dos Santos Alves, Taila dos Santos Alves, Taila |
title_short |
Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis |
title_full |
Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis |
title_fullStr |
Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis |
title_full_unstemmed |
Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis |
title_sort |
Genome-Based Characterization of Multidrug-Resistant Escherichia coli Isolated from Clinical Bovine Mastitis |
author |
dos Santos Alves, Taila |
author_facet |
dos Santos Alves, Taila dos Santos Alves, Taila Rosa, Vinícius Sanches da Silva Leite, Domingos Guerra, Simony Trevizan [UNESP] Joaquim, Sâmea Fernandes [UNESP] Guimarães, Felipe Freitas [UNESP] de Figueiredo Pantoja, José Carlos [UNESP] Lucheis, Simoni Baldini [UNESP] Rall, Vera Lúcia Mores [UNESP] Hernandes, Rodrigo Tavanelli [UNESP] Langoni, Helio [UNESP] Ribeiro, Márcio Garcia [UNESP] Rosa, Vinícius Sanches da Silva Leite, Domingos Guerra, Simony Trevizan [UNESP] Joaquim, Sâmea Fernandes [UNESP] Guimarães, Felipe Freitas [UNESP] de Figueiredo Pantoja, José Carlos [UNESP] Lucheis, Simoni Baldini [UNESP] Rall, Vera Lúcia Mores [UNESP] Hernandes, Rodrigo Tavanelli [UNESP] Langoni, Helio [UNESP] Ribeiro, Márcio Garcia [UNESP] |
author_role |
author |
author2 |
Rosa, Vinícius Sanches da Silva Leite, Domingos Guerra, Simony Trevizan [UNESP] Joaquim, Sâmea Fernandes [UNESP] Guimarães, Felipe Freitas [UNESP] de Figueiredo Pantoja, José Carlos [UNESP] Lucheis, Simoni Baldini [UNESP] Rall, Vera Lúcia Mores [UNESP] Hernandes, Rodrigo Tavanelli [UNESP] Langoni, Helio [UNESP] Ribeiro, Márcio Garcia [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
dos Santos Alves, Taila Rosa, Vinícius Sanches da Silva Leite, Domingos Guerra, Simony Trevizan [UNESP] Joaquim, Sâmea Fernandes [UNESP] Guimarães, Felipe Freitas [UNESP] de Figueiredo Pantoja, José Carlos [UNESP] Lucheis, Simoni Baldini [UNESP] Rall, Vera Lúcia Mores [UNESP] Hernandes, Rodrigo Tavanelli [UNESP] Langoni, Helio [UNESP] Ribeiro, Márcio Garcia [UNESP] |
description |
Mastitis occurrence in dairy cows is a broad topic that involves several sectors, from antimicrobial resistance and virulence of strains to economic implications and cattle management practices. Here, we assessed the molecular characterization (antimicrobial resistance determinants, virulence genes, sequences type, serotypes, and plasmid types) of 178 Escherichia coli strains isolated from milk samples from cows with clinical mastitis using a genome-based k-mers approach. Of these, 53 (29.8%) showed multidrug resistance by disc diffusion. We selected eight multidrug-resistant mastitis-associated E. coli for whole-genome sequencing and molecular characterization based on raw data using k-mers. We assessed antimicrobial resistance genes, virulence factors, serotypes, Multilocus Sequence Typing (MLST), and plasmid types. The most antimicrobial resistance gene found were blaTEM-1B (7/8), tetA (6/8), strA (6/8), strB (6/8), and qnrB19 (5/8). A total of 25 virulence factors were detected encoding adhesins, capsule, enzymes/proteins, increased serum survival, hemolysin, colicins, and iron uptake. These virulence factors were associated with Extraintestinal Pathogenic E. coli. Three pandemic clones were found: ST10, ST101, and ST69. Two E. coli were assigned in the O117 serogroup and one in the O8:H25 serotype. The most common plasmid groups were IncFII (7/8) and IncFIB (6/8). Our findings contribute to the knowledge of virulence mechanisms, epidemiological aspects, and antimicrobial resistance determinants of E. coli strains obtained from clinical mammary infections of cows. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:39:41Z 2023-07-29T13:39:41Z 2023-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00284-023-03191-6 Current Microbiology, v. 80, n. 3, 2023. 1432-0991 0343-8651 http://hdl.handle.net/11449/248288 10.1007/s00284-023-03191-6 2-s2.0-85147178135 |
url |
http://dx.doi.org/10.1007/s00284-023-03191-6 http://hdl.handle.net/11449/248288 |
identifier_str_mv |
Current Microbiology, v. 80, n. 3, 2023. 1432-0991 0343-8651 10.1007/s00284-023-03191-6 2-s2.0-85147178135 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Current Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1822183849565618176 |
dc.identifier.doi.none.fl_str_mv |
10.1007/s00284-023-03191-6 |