Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birds
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fphys.2022.892828 http://hdl.handle.net/11449/242099 |
Resumo: | Transient receptor potential vanilloid 4 (TRPV4) channels are sensitive to warm ambient temperatures (Tas), triggering heat loss responses in adult rats in a Tas range of ∼26–30°C. In birds, however, the thermoregulatory role of TRPV4 has never been shown. Here, we hypothesized that stimulation of TRPV4 induces thermolytic responses for body temperature (Tb) maintenance in birds, and that this function is already present in early life, when the Ta range for TRPV4 activation does not represent a warm condition for these animals. We first demonstrated the presence of TRPV4 in the dorsal and ventral skin of chickens (Gallus gallus domesticus) by immunohistochemistry. Then, we evaluated the effects of the TRPV4 agonist, RN1747, and the TRPV4 antagonists, HC067047 and GSK2193874, on Tb and thermoeffectors at different Tas in 5-day-old chicks and 60-day-old adult chickens. For the chicks, RN1747 transiently reduced Tb both in thermoneutrality (31°C) and in a cold Ta for this phase (26°C), which relied on huddling behavior inhibition. The TRPV4 antagonists alone did not affect Tb or thermoeffectors but blocked the Tb decrease and huddling inhibition promoted by RN1747. For the adults, TRPV4 antagonism increased Tb when animals were exposed to 28°C (suprathermoneutral condition for adults), but not to 19°C. In contrast, RN1747 decreased Tb by reducing metabolic rate and activating thermal tachypnea at 19°C, a Ta below the activation range of TRPV4. Our results indicate that peripheral TRPV4 receptors are functional in early life, but may be inhibited at that time when the range of activation (∼26–30°C) represents cold Ta for chicks, and become physiologically relevant for Tb maintenance when the activation Ta range for TRPV4 becomes suprathermoneutral for adult chickens. |
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Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birdsbody temperaturechickenhuddlingMetabolismperipheral thermoreceptorthermolysisthermoregulationTransient receptor potential vanilloid 4 (TRPV4) channels are sensitive to warm ambient temperatures (Tas), triggering heat loss responses in adult rats in a Tas range of ∼26–30°C. In birds, however, the thermoregulatory role of TRPV4 has never been shown. Here, we hypothesized that stimulation of TRPV4 induces thermolytic responses for body temperature (Tb) maintenance in birds, and that this function is already present in early life, when the Ta range for TRPV4 activation does not represent a warm condition for these animals. We first demonstrated the presence of TRPV4 in the dorsal and ventral skin of chickens (Gallus gallus domesticus) by immunohistochemistry. Then, we evaluated the effects of the TRPV4 agonist, RN1747, and the TRPV4 antagonists, HC067047 and GSK2193874, on Tb and thermoeffectors at different Tas in 5-day-old chicks and 60-day-old adult chickens. For the chicks, RN1747 transiently reduced Tb both in thermoneutrality (31°C) and in a cold Ta for this phase (26°C), which relied on huddling behavior inhibition. The TRPV4 antagonists alone did not affect Tb or thermoeffectors but blocked the Tb decrease and huddling inhibition promoted by RN1747. For the adults, TRPV4 antagonism increased Tb when animals were exposed to 28°C (suprathermoneutral condition for adults), but not to 19°C. In contrast, RN1747 decreased Tb by reducing metabolic rate and activating thermal tachypnea at 19°C, a Ta below the activation range of TRPV4. Our results indicate that peripheral TRPV4 receptors are functional in early life, but may be inhibited at that time when the range of activation (∼26–30°C) represents cold Ta for chicks, and become physiologically relevant for Tb maintenance when the activation Ta range for TRPV4 becomes suprathermoneutral for adult chickens.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Animal Morphology and Physiology Faculty of Agricultural and Veterinary Sciences Sao Paulo State UniversityDepartment of Animal Science Faculty of Agricultural and Veterinary Sciences Sao Paulo State UniversityInstitute of Biological Sciences Department of Physiology and Biophysics Federal University of Minas Gerais (ICB/UFMG)Center for Natural and Human Sciences Federal University of ABC (UFABC)Department of Animal Morphology and Physiology Faculty of Agricultural and Veterinary Sciences Sao Paulo State UniversityDepartment of Animal Science Faculty of Agricultural and Veterinary Sciences Sao Paulo State UniversityFAPESP: 15/04849-6 17/12627-9Universidade Estadual Paulista (UNESP)Universidade Federal de Minas Gerais (UFMG)Universidade Federal do ABC (UFABC)Cristina-Silva, Caroline [UNESP]Amaral-Silva, Lara [UNESP]Santos, Kassia Moreira [UNESP]Correa, Gabriela Monteiro [UNESP]da Silva, Welex Candido [UNESP]Fernandes, Marcia H. M. R. [UNESP]da Silva, Glauber S. F.Gargaglioni, Luciane H. [UNESP]Almeida, Maria C.Bicego, Kenia C. [UNESP]2023-03-02T08:38:24Z2023-03-02T08:38:24Z2022-07-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fphys.2022.892828Frontiers in Physiology, v. 13.1664-042Xhttp://hdl.handle.net/11449/24209910.3389/fphys.2022.8928282-s2.0-85135199153Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Physiologyinfo:eu-repo/semantics/openAccess2024-06-06T18:41:54Zoai:repositorio.unesp.br:11449/242099Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:39:52.069764Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birds |
title |
Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birds |
spellingShingle |
Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birds Cristina-Silva, Caroline [UNESP] body temperature chicken huddling Metabolism peripheral thermoreceptor thermolysis thermoregulation |
title_short |
Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birds |
title_full |
Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birds |
title_fullStr |
Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birds |
title_full_unstemmed |
Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birds |
title_sort |
Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birds |
author |
Cristina-Silva, Caroline [UNESP] |
author_facet |
Cristina-Silva, Caroline [UNESP] Amaral-Silva, Lara [UNESP] Santos, Kassia Moreira [UNESP] Correa, Gabriela Monteiro [UNESP] da Silva, Welex Candido [UNESP] Fernandes, Marcia H. M. R. [UNESP] da Silva, Glauber S. F. Gargaglioni, Luciane H. [UNESP] Almeida, Maria C. Bicego, Kenia C. [UNESP] |
author_role |
author |
author2 |
Amaral-Silva, Lara [UNESP] Santos, Kassia Moreira [UNESP] Correa, Gabriela Monteiro [UNESP] da Silva, Welex Candido [UNESP] Fernandes, Marcia H. M. R. [UNESP] da Silva, Glauber S. F. Gargaglioni, Luciane H. [UNESP] Almeida, Maria C. Bicego, Kenia C. [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Federal de Minas Gerais (UFMG) Universidade Federal do ABC (UFABC) |
dc.contributor.author.fl_str_mv |
Cristina-Silva, Caroline [UNESP] Amaral-Silva, Lara [UNESP] Santos, Kassia Moreira [UNESP] Correa, Gabriela Monteiro [UNESP] da Silva, Welex Candido [UNESP] Fernandes, Marcia H. M. R. [UNESP] da Silva, Glauber S. F. Gargaglioni, Luciane H. [UNESP] Almeida, Maria C. Bicego, Kenia C. [UNESP] |
dc.subject.por.fl_str_mv |
body temperature chicken huddling Metabolism peripheral thermoreceptor thermolysis thermoregulation |
topic |
body temperature chicken huddling Metabolism peripheral thermoreceptor thermolysis thermoregulation |
description |
Transient receptor potential vanilloid 4 (TRPV4) channels are sensitive to warm ambient temperatures (Tas), triggering heat loss responses in adult rats in a Tas range of ∼26–30°C. In birds, however, the thermoregulatory role of TRPV4 has never been shown. Here, we hypothesized that stimulation of TRPV4 induces thermolytic responses for body temperature (Tb) maintenance in birds, and that this function is already present in early life, when the Ta range for TRPV4 activation does not represent a warm condition for these animals. We first demonstrated the presence of TRPV4 in the dorsal and ventral skin of chickens (Gallus gallus domesticus) by immunohistochemistry. Then, we evaluated the effects of the TRPV4 agonist, RN1747, and the TRPV4 antagonists, HC067047 and GSK2193874, on Tb and thermoeffectors at different Tas in 5-day-old chicks and 60-day-old adult chickens. For the chicks, RN1747 transiently reduced Tb both in thermoneutrality (31°C) and in a cold Ta for this phase (26°C), which relied on huddling behavior inhibition. The TRPV4 antagonists alone did not affect Tb or thermoeffectors but blocked the Tb decrease and huddling inhibition promoted by RN1747. For the adults, TRPV4 antagonism increased Tb when animals were exposed to 28°C (suprathermoneutral condition for adults), but not to 19°C. In contrast, RN1747 decreased Tb by reducing metabolic rate and activating thermal tachypnea at 19°C, a Ta below the activation range of TRPV4. Our results indicate that peripheral TRPV4 receptors are functional in early life, but may be inhibited at that time when the range of activation (∼26–30°C) represents cold Ta for chicks, and become physiologically relevant for Tb maintenance when the activation Ta range for TRPV4 becomes suprathermoneutral for adult chickens. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-15 2023-03-02T08:38:24Z 2023-03-02T08:38:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fphys.2022.892828 Frontiers in Physiology, v. 13. 1664-042X http://hdl.handle.net/11449/242099 10.3389/fphys.2022.892828 2-s2.0-85135199153 |
url |
http://dx.doi.org/10.3389/fphys.2022.892828 http://hdl.handle.net/11449/242099 |
identifier_str_mv |
Frontiers in Physiology, v. 13. 1664-042X 10.3389/fphys.2022.892828 2-s2.0-85135199153 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Physiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129103958638592 |