Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birds

Detalhes bibliográficos
Autor(a) principal: Cristina-Silva, Caroline [UNESP]
Data de Publicação: 2022
Outros Autores: Amaral-Silva, Lara [UNESP], Santos, Kassia Moreira [UNESP], Correa, Gabriela Monteiro [UNESP], da Silva, Welex Candido [UNESP], Fernandes, Marcia H. M. R. [UNESP], da Silva, Glauber S. F., Gargaglioni, Luciane H. [UNESP], Almeida, Maria C., Bicego, Kenia C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fphys.2022.892828
http://hdl.handle.net/11449/242099
Resumo: Transient receptor potential vanilloid 4 (TRPV4) channels are sensitive to warm ambient temperatures (Tas), triggering heat loss responses in adult rats in a Tas range of ∼26–30°C. In birds, however, the thermoregulatory role of TRPV4 has never been shown. Here, we hypothesized that stimulation of TRPV4 induces thermolytic responses for body temperature (Tb) maintenance in birds, and that this function is already present in early life, when the Ta range for TRPV4 activation does not represent a warm condition for these animals. We first demonstrated the presence of TRPV4 in the dorsal and ventral skin of chickens (Gallus gallus domesticus) by immunohistochemistry. Then, we evaluated the effects of the TRPV4 agonist, RN1747, and the TRPV4 antagonists, HC067047 and GSK2193874, on Tb and thermoeffectors at different Tas in 5-day-old chicks and 60-day-old adult chickens. For the chicks, RN1747 transiently reduced Tb both in thermoneutrality (31°C) and in a cold Ta for this phase (26°C), which relied on huddling behavior inhibition. The TRPV4 antagonists alone did not affect Tb or thermoeffectors but blocked the Tb decrease and huddling inhibition promoted by RN1747. For the adults, TRPV4 antagonism increased Tb when animals were exposed to 28°C (suprathermoneutral condition for adults), but not to 19°C. In contrast, RN1747 decreased Tb by reducing metabolic rate and activating thermal tachypnea at 19°C, a Ta below the activation range of TRPV4. Our results indicate that peripheral TRPV4 receptors are functional in early life, but may be inhibited at that time when the range of activation (∼26–30°C) represents cold Ta for chicks, and become physiologically relevant for Tb maintenance when the activation Ta range for TRPV4 becomes suprathermoneutral for adult chickens.
id UNSP_d2a941085af6e8a734c30d9a7cc346ec
oai_identifier_str oai:repositorio.unesp.br:11449/242099
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birdsbody temperaturechickenhuddlingMetabolismperipheral thermoreceptorthermolysisthermoregulationTransient receptor potential vanilloid 4 (TRPV4) channels are sensitive to warm ambient temperatures (Tas), triggering heat loss responses in adult rats in a Tas range of ∼26–30°C. In birds, however, the thermoregulatory role of TRPV4 has never been shown. Here, we hypothesized that stimulation of TRPV4 induces thermolytic responses for body temperature (Tb) maintenance in birds, and that this function is already present in early life, when the Ta range for TRPV4 activation does not represent a warm condition for these animals. We first demonstrated the presence of TRPV4 in the dorsal and ventral skin of chickens (Gallus gallus domesticus) by immunohistochemistry. Then, we evaluated the effects of the TRPV4 agonist, RN1747, and the TRPV4 antagonists, HC067047 and GSK2193874, on Tb and thermoeffectors at different Tas in 5-day-old chicks and 60-day-old adult chickens. For the chicks, RN1747 transiently reduced Tb both in thermoneutrality (31°C) and in a cold Ta for this phase (26°C), which relied on huddling behavior inhibition. The TRPV4 antagonists alone did not affect Tb or thermoeffectors but blocked the Tb decrease and huddling inhibition promoted by RN1747. For the adults, TRPV4 antagonism increased Tb when animals were exposed to 28°C (suprathermoneutral condition for adults), but not to 19°C. In contrast, RN1747 decreased Tb by reducing metabolic rate and activating thermal tachypnea at 19°C, a Ta below the activation range of TRPV4. Our results indicate that peripheral TRPV4 receptors are functional in early life, but may be inhibited at that time when the range of activation (∼26–30°C) represents cold Ta for chicks, and become physiologically relevant for Tb maintenance when the activation Ta range for TRPV4 becomes suprathermoneutral for adult chickens.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Animal Morphology and Physiology Faculty of Agricultural and Veterinary Sciences Sao Paulo State UniversityDepartment of Animal Science Faculty of Agricultural and Veterinary Sciences Sao Paulo State UniversityInstitute of Biological Sciences Department of Physiology and Biophysics Federal University of Minas Gerais (ICB/UFMG)Center for Natural and Human Sciences Federal University of ABC (UFABC)Department of Animal Morphology and Physiology Faculty of Agricultural and Veterinary Sciences Sao Paulo State UniversityDepartment of Animal Science Faculty of Agricultural and Veterinary Sciences Sao Paulo State UniversityFAPESP: 15/04849-6 17/12627-9Universidade Estadual Paulista (UNESP)Universidade Federal de Minas Gerais (UFMG)Universidade Federal do ABC (UFABC)Cristina-Silva, Caroline [UNESP]Amaral-Silva, Lara [UNESP]Santos, Kassia Moreira [UNESP]Correa, Gabriela Monteiro [UNESP]da Silva, Welex Candido [UNESP]Fernandes, Marcia H. M. R. [UNESP]da Silva, Glauber S. F.Gargaglioni, Luciane H. [UNESP]Almeida, Maria C.Bicego, Kenia C. [UNESP]2023-03-02T08:38:24Z2023-03-02T08:38:24Z2022-07-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fphys.2022.892828Frontiers in Physiology, v. 13.1664-042Xhttp://hdl.handle.net/11449/24209910.3389/fphys.2022.8928282-s2.0-85135199153Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Physiologyinfo:eu-repo/semantics/openAccess2024-06-06T18:41:54Zoai:repositorio.unesp.br:11449/242099Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:39:52.069764Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birds
title Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birds
spellingShingle Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birds
Cristina-Silva, Caroline [UNESP]
body temperature
chicken
huddling
Metabolism
peripheral thermoreceptor
thermolysis
thermoregulation
title_short Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birds
title_full Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birds
title_fullStr Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birds
title_full_unstemmed Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birds
title_sort Cutaneous TRPV4 Channels Activate Warmth-Defense Responses in Young and Adult Birds
author Cristina-Silva, Caroline [UNESP]
author_facet Cristina-Silva, Caroline [UNESP]
Amaral-Silva, Lara [UNESP]
Santos, Kassia Moreira [UNESP]
Correa, Gabriela Monteiro [UNESP]
da Silva, Welex Candido [UNESP]
Fernandes, Marcia H. M. R. [UNESP]
da Silva, Glauber S. F.
Gargaglioni, Luciane H. [UNESP]
Almeida, Maria C.
Bicego, Kenia C. [UNESP]
author_role author
author2 Amaral-Silva, Lara [UNESP]
Santos, Kassia Moreira [UNESP]
Correa, Gabriela Monteiro [UNESP]
da Silva, Welex Candido [UNESP]
Fernandes, Marcia H. M. R. [UNESP]
da Silva, Glauber S. F.
Gargaglioni, Luciane H. [UNESP]
Almeida, Maria C.
Bicego, Kenia C. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Federal de Minas Gerais (UFMG)
Universidade Federal do ABC (UFABC)
dc.contributor.author.fl_str_mv Cristina-Silva, Caroline [UNESP]
Amaral-Silva, Lara [UNESP]
Santos, Kassia Moreira [UNESP]
Correa, Gabriela Monteiro [UNESP]
da Silva, Welex Candido [UNESP]
Fernandes, Marcia H. M. R. [UNESP]
da Silva, Glauber S. F.
Gargaglioni, Luciane H. [UNESP]
Almeida, Maria C.
Bicego, Kenia C. [UNESP]
dc.subject.por.fl_str_mv body temperature
chicken
huddling
Metabolism
peripheral thermoreceptor
thermolysis
thermoregulation
topic body temperature
chicken
huddling
Metabolism
peripheral thermoreceptor
thermolysis
thermoregulation
description Transient receptor potential vanilloid 4 (TRPV4) channels are sensitive to warm ambient temperatures (Tas), triggering heat loss responses in adult rats in a Tas range of ∼26–30°C. In birds, however, the thermoregulatory role of TRPV4 has never been shown. Here, we hypothesized that stimulation of TRPV4 induces thermolytic responses for body temperature (Tb) maintenance in birds, and that this function is already present in early life, when the Ta range for TRPV4 activation does not represent a warm condition for these animals. We first demonstrated the presence of TRPV4 in the dorsal and ventral skin of chickens (Gallus gallus domesticus) by immunohistochemistry. Then, we evaluated the effects of the TRPV4 agonist, RN1747, and the TRPV4 antagonists, HC067047 and GSK2193874, on Tb and thermoeffectors at different Tas in 5-day-old chicks and 60-day-old adult chickens. For the chicks, RN1747 transiently reduced Tb both in thermoneutrality (31°C) and in a cold Ta for this phase (26°C), which relied on huddling behavior inhibition. The TRPV4 antagonists alone did not affect Tb or thermoeffectors but blocked the Tb decrease and huddling inhibition promoted by RN1747. For the adults, TRPV4 antagonism increased Tb when animals were exposed to 28°C (suprathermoneutral condition for adults), but not to 19°C. In contrast, RN1747 decreased Tb by reducing metabolic rate and activating thermal tachypnea at 19°C, a Ta below the activation range of TRPV4. Our results indicate that peripheral TRPV4 receptors are functional in early life, but may be inhibited at that time when the range of activation (∼26–30°C) represents cold Ta for chicks, and become physiologically relevant for Tb maintenance when the activation Ta range for TRPV4 becomes suprathermoneutral for adult chickens.
publishDate 2022
dc.date.none.fl_str_mv 2022-07-15
2023-03-02T08:38:24Z
2023-03-02T08:38:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fphys.2022.892828
Frontiers in Physiology, v. 13.
1664-042X
http://hdl.handle.net/11449/242099
10.3389/fphys.2022.892828
2-s2.0-85135199153
url http://dx.doi.org/10.3389/fphys.2022.892828
http://hdl.handle.net/11449/242099
identifier_str_mv Frontiers in Physiology, v. 13.
1664-042X
10.3389/fphys.2022.892828
2-s2.0-85135199153
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Physiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129103958638592