Biocompatibility of primers and an adhesive used for implant-retained maxillofacial prostheses: An in vitro analysis

Detalhes bibliográficos
Autor(a) principal: Bonatto, Liliane da Rocha [UNESP]
Data de Publicação: 2017
Outros Autores: Goiato, Marcelo Coelho [UNESP], Freitas da Silva, Emily Vivianne [UNESP], Penha Oliveira, Sandra Helena [UNESP], Haddad, Marcela Filie, Chaves Neto, Antonio Hernandes [UNESP], Balera Brito, Victor Gustavo [UNESP], Santos, Daniela Micheline dos [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/162873
Resumo: Statement of problem. Implant-retained maxillofacial prostheses should be biocompatible, regardless of the primers and adhesives used to bond the acrylic resin and facial silicone. The authors are unaware of any study evaluating the influence of these primers and adhesives on the biocompatibility of maxillofacial prostheses. Purpose. The purpose of this in vitro study was to evaluate the cytotoxic effect of primers and an adhesive used to bond acrylic resin and facial silicone during the fabrication of implant-retained maxillofacial prostheses. Material and methods. Twenty-eight circular specimens made of resin and silicone were fabricated, either bonded or nonbonded with primer and adhesive. The specimens were divided into 7 groups: resin; silicone; resin+silastic medical adhesive type A+silicone; resin+DC 1205 primer silicone; resin+Sofreliner primer+silicone; resin+DC 1205 primer+silastic medical adhesive type A+silicone; and resin+Sofreliner primer+silastic medical adhesive type A+silicone. Eluates of the materials tested were prepared by setting 4 specimens of each experimental group in Falcon tubes with medium and incubating at 37 degrees C for 24 hours. The eluate cytotoxicity was evaluated by an assay of survival/proliferation ((314,5-dimethylthiazol-2-y1)-2,5-diphenyl tetrazolium bromide [MTT] test) in cultures of human keratinotytes. The levels of IL1, IL6, TNF alpha, and the chemokine MIP-1 alpha. were evaluated by enzyme-linked immunosorbent assay. The mRNA expressions for MMP-9, TGF-beta, and collagen type IV were analyzed by the regtime polymerase chain reaction. Data were submitted to analysis of variance with Bonferroni post hoc tests (alpha=.05). Results. An increased cell proliferation was observed for the RAS group, with statistically significant differences (P<.001) compared with the unstimulated group. The RDCpS group showed the highest IL6 concentration values (P<.001). No significant statistical difference was found in the relative quantification of mRNA for collagen type IV, MMP9, or TGF beta between the groups (P>.05). Conclusions. The RAS group showed the highest cell proliferation percentage, while the RDCpS group exhibited the highest IL6 concentration values. No detectable levels of iL beta, TNF alpha, or CCL3/MIP1 alpha were observed. The tested materials showed no toxic effects on the HaCaT cell line.
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spelling Biocompatibility of primers and an adhesive used for implant-retained maxillofacial prostheses: An in vitro analysisStatement of problem. Implant-retained maxillofacial prostheses should be biocompatible, regardless of the primers and adhesives used to bond the acrylic resin and facial silicone. The authors are unaware of any study evaluating the influence of these primers and adhesives on the biocompatibility of maxillofacial prostheses. Purpose. The purpose of this in vitro study was to evaluate the cytotoxic effect of primers and an adhesive used to bond acrylic resin and facial silicone during the fabrication of implant-retained maxillofacial prostheses. Material and methods. Twenty-eight circular specimens made of resin and silicone were fabricated, either bonded or nonbonded with primer and adhesive. The specimens were divided into 7 groups: resin; silicone; resin+silastic medical adhesive type A+silicone; resin+DC 1205 primer silicone; resin+Sofreliner primer+silicone; resin+DC 1205 primer+silastic medical adhesive type A+silicone; and resin+Sofreliner primer+silastic medical adhesive type A+silicone. Eluates of the materials tested were prepared by setting 4 specimens of each experimental group in Falcon tubes with medium and incubating at 37 degrees C for 24 hours. The eluate cytotoxicity was evaluated by an assay of survival/proliferation ((314,5-dimethylthiazol-2-y1)-2,5-diphenyl tetrazolium bromide [MTT] test) in cultures of human keratinotytes. The levels of IL1, IL6, TNF alpha, and the chemokine MIP-1 alpha. were evaluated by enzyme-linked immunosorbent assay. The mRNA expressions for MMP-9, TGF-beta, and collagen type IV were analyzed by the regtime polymerase chain reaction. Data were submitted to analysis of variance with Bonferroni post hoc tests (alpha=.05). Results. An increased cell proliferation was observed for the RAS group, with statistically significant differences (P<.001) compared with the unstimulated group. The RDCpS group showed the highest IL6 concentration values (P<.001). No significant statistical difference was found in the relative quantification of mRNA for collagen type IV, MMP9, or TGF beta between the groups (P>.05). Conclusions. The RAS group showed the highest cell proliferation percentage, while the RDCpS group exhibited the highest IL6 concentration values. No detectable levels of iL beta, TNF alpha, or CCL3/MIP1 alpha were observed. The tested materials showed no toxic effects on the HaCaT cell line.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Brazilian National Research CouncilSao Paulo State Univ, Aracatuba Dent Sch, Dept Dent Mat & Prosthodont, Sao Paulo, BrazilSao Paulo State Univ, Aracatuba Dent Sch, Dept Basic Sci, Sao Paulo, BrazilUniv Fed Alfenas, Dept Dent Mat & Prosthodont, Alfenas, MG, BrazilSao Paulo State Univ, Aracatuba Dent Sch, Dept Dent Mat & Prosthodont, Sao Paulo, BrazilSao Paulo State Univ, Aracatuba Dent Sch, Dept Basic Sci, Sao Paulo, BrazilFAPESP: 2012/02907-0Brazilian National Research Council: 305555/2013-8Elsevier B.V.Universidade Estadual Paulista (Unesp)Univ Fed AlfenasBonatto, Liliane da Rocha [UNESP]Goiato, Marcelo Coelho [UNESP]Freitas da Silva, Emily Vivianne [UNESP]Penha Oliveira, Sandra Helena [UNESP]Haddad, Marcela FilieChaves Neto, Antonio Hernandes [UNESP]Balera Brito, Victor Gustavo [UNESP]Santos, Daniela Micheline dos [UNESP]2018-11-26T17:34:46Z2018-11-26T17:34:46Z2017-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article799-805application/pdfJournal Of Prosthetic Dentistry. New York: Mosby-elsevier, v. 117, n. 6, p. 799-805, 2017.0022-3913http://hdl.handle.net/11449/162873WOS:000403197400018WOS000403197400018.pdf9719883814872582Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Prosthetic Dentistry1,087info:eu-repo/semantics/openAccess2024-09-19T14:51:58Zoai:repositorio.unesp.br:11449/162873Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T14:51:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Biocompatibility of primers and an adhesive used for implant-retained maxillofacial prostheses: An in vitro analysis
title Biocompatibility of primers and an adhesive used for implant-retained maxillofacial prostheses: An in vitro analysis
spellingShingle Biocompatibility of primers and an adhesive used for implant-retained maxillofacial prostheses: An in vitro analysis
Bonatto, Liliane da Rocha [UNESP]
title_short Biocompatibility of primers and an adhesive used for implant-retained maxillofacial prostheses: An in vitro analysis
title_full Biocompatibility of primers and an adhesive used for implant-retained maxillofacial prostheses: An in vitro analysis
title_fullStr Biocompatibility of primers and an adhesive used for implant-retained maxillofacial prostheses: An in vitro analysis
title_full_unstemmed Biocompatibility of primers and an adhesive used for implant-retained maxillofacial prostheses: An in vitro analysis
title_sort Biocompatibility of primers and an adhesive used for implant-retained maxillofacial prostheses: An in vitro analysis
author Bonatto, Liliane da Rocha [UNESP]
author_facet Bonatto, Liliane da Rocha [UNESP]
Goiato, Marcelo Coelho [UNESP]
Freitas da Silva, Emily Vivianne [UNESP]
Penha Oliveira, Sandra Helena [UNESP]
Haddad, Marcela Filie
Chaves Neto, Antonio Hernandes [UNESP]
Balera Brito, Victor Gustavo [UNESP]
Santos, Daniela Micheline dos [UNESP]
author_role author
author2 Goiato, Marcelo Coelho [UNESP]
Freitas da Silva, Emily Vivianne [UNESP]
Penha Oliveira, Sandra Helena [UNESP]
Haddad, Marcela Filie
Chaves Neto, Antonio Hernandes [UNESP]
Balera Brito, Victor Gustavo [UNESP]
Santos, Daniela Micheline dos [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Univ Fed Alfenas
dc.contributor.author.fl_str_mv Bonatto, Liliane da Rocha [UNESP]
Goiato, Marcelo Coelho [UNESP]
Freitas da Silva, Emily Vivianne [UNESP]
Penha Oliveira, Sandra Helena [UNESP]
Haddad, Marcela Filie
Chaves Neto, Antonio Hernandes [UNESP]
Balera Brito, Victor Gustavo [UNESP]
Santos, Daniela Micheline dos [UNESP]
description Statement of problem. Implant-retained maxillofacial prostheses should be biocompatible, regardless of the primers and adhesives used to bond the acrylic resin and facial silicone. The authors are unaware of any study evaluating the influence of these primers and adhesives on the biocompatibility of maxillofacial prostheses. Purpose. The purpose of this in vitro study was to evaluate the cytotoxic effect of primers and an adhesive used to bond acrylic resin and facial silicone during the fabrication of implant-retained maxillofacial prostheses. Material and methods. Twenty-eight circular specimens made of resin and silicone were fabricated, either bonded or nonbonded with primer and adhesive. The specimens were divided into 7 groups: resin; silicone; resin+silastic medical adhesive type A+silicone; resin+DC 1205 primer silicone; resin+Sofreliner primer+silicone; resin+DC 1205 primer+silastic medical adhesive type A+silicone; and resin+Sofreliner primer+silastic medical adhesive type A+silicone. Eluates of the materials tested were prepared by setting 4 specimens of each experimental group in Falcon tubes with medium and incubating at 37 degrees C for 24 hours. The eluate cytotoxicity was evaluated by an assay of survival/proliferation ((314,5-dimethylthiazol-2-y1)-2,5-diphenyl tetrazolium bromide [MTT] test) in cultures of human keratinotytes. The levels of IL1, IL6, TNF alpha, and the chemokine MIP-1 alpha. were evaluated by enzyme-linked immunosorbent assay. The mRNA expressions for MMP-9, TGF-beta, and collagen type IV were analyzed by the regtime polymerase chain reaction. Data were submitted to analysis of variance with Bonferroni post hoc tests (alpha=.05). Results. An increased cell proliferation was observed for the RAS group, with statistically significant differences (P<.001) compared with the unstimulated group. The RDCpS group showed the highest IL6 concentration values (P<.001). No significant statistical difference was found in the relative quantification of mRNA for collagen type IV, MMP9, or TGF beta between the groups (P>.05). Conclusions. The RAS group showed the highest cell proliferation percentage, while the RDCpS group exhibited the highest IL6 concentration values. No detectable levels of iL beta, TNF alpha, or CCL3/MIP1 alpha were observed. The tested materials showed no toxic effects on the HaCaT cell line.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-01
2018-11-26T17:34:46Z
2018-11-26T17:34:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Journal Of Prosthetic Dentistry. New York: Mosby-elsevier, v. 117, n. 6, p. 799-805, 2017.
0022-3913
http://hdl.handle.net/11449/162873
WOS:000403197400018
WOS000403197400018.pdf
9719883814872582
identifier_str_mv Journal Of Prosthetic Dentistry. New York: Mosby-elsevier, v. 117, n. 6, p. 799-805, 2017.
0022-3913
WOS:000403197400018
WOS000403197400018.pdf
9719883814872582
url http://hdl.handle.net/11449/162873
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Prosthetic Dentistry
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dc.format.none.fl_str_mv 799-805
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
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instname_str Universidade Estadual Paulista (UNESP)
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reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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