In vivo toxicogenic potential of Salix alba (Salicaceae) bark extract

Detalhes bibliográficos
Autor(a) principal: Maistro, Edson Luis [UNESP]
Data de Publicação: 2022
Outros Autores: Terrazzas, Peterson Menezes [UNESP], Sawaya, Alexandra Christinie Helena Frankland, Rosa, Paulo Cesar Pires, Perazzo, Fábio Ferreira, de Mascarenhas Gaivão, Isabel O’Neill
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1080/15287394.2021.1989351
http://hdl.handle.net/11449/233724
Resumo: Salix alba (white willow) bark extract is widely used for conditions associated with inflammation, fever, microbial infection or pain. Exposure of human cultured leukocytes to S. alba in vitro noted a genotoxic response. However, data regarding the influence of this bark extract on DNA damage in vivo are lacking. The main goal of this study was to examine the potential of S.alba bark extract to induce DNA damage and chromosome aberrations in an in vivo model using cells obtained from male Swiss albino mice administered the compound orally. The extract was administered by oral gavage daily for 7 days at doses of 500, 1000, or 2000 mg/kg b.w. Genotoxicity analysis was performed using the comet assay on peripheral blood leukocytes, as well as liver, bone marrow, heart, and testicular cells collected 4 hr after the last treatment and the micronucleus (MN) test on bone marrow cells. In essence cells were collected 28 hr after the penultimate treatment Data demonstrated that S. alba bark extract did not induce significant DNA damage in any cell types examined, or clastogenic/aneugenic effects as detected by the MN test at the three tested doses. Under these experimental conditions, evidence indicates that S.alba bark extract did not initiate genotoxic or chromosome aberrations in various mouse cells investigated.
id UNSP_d357636d163dcc20f622c4b8dab3db73
oai_identifier_str oai:repositorio.unesp.br:11449/233724
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling In vivo toxicogenic potential of Salix alba (Salicaceae) bark extractSalicaceae genotoxicitySalix comet assaySalix micronucleus testWhite willowSalix alba (white willow) bark extract is widely used for conditions associated with inflammation, fever, microbial infection or pain. Exposure of human cultured leukocytes to S. alba in vitro noted a genotoxic response. However, data regarding the influence of this bark extract on DNA damage in vivo are lacking. The main goal of this study was to examine the potential of S.alba bark extract to induce DNA damage and chromosome aberrations in an in vivo model using cells obtained from male Swiss albino mice administered the compound orally. The extract was administered by oral gavage daily for 7 days at doses of 500, 1000, or 2000 mg/kg b.w. Genotoxicity analysis was performed using the comet assay on peripheral blood leukocytes, as well as liver, bone marrow, heart, and testicular cells collected 4 hr after the last treatment and the micronucleus (MN) test on bone marrow cells. In essence cells were collected 28 hr after the penultimate treatment Data demonstrated that S. alba bark extract did not induce significant DNA damage in any cell types examined, or clastogenic/aneugenic effects as detected by the MN test at the three tested doses. Under these experimental conditions, evidence indicates that S.alba bark extract did not initiate genotoxic or chromosome aberrations in various mouse cells investigated.Speech and Hearing Therapy Department São Paulo State University - UnespPrograma De Pós-Graduação Em Biologia Geral E Aplicada–São Paulo State University–Unesp Instituto De BiociênciasFaculty of Pharmaceutical Sciences University of CampinasDepartment of Genetics and Biotechnology and Animal and Veterinary Research Centre (CECAV) University of Trás-os-Montes and Alto Douro (Utad)Universidade De Tras-os-Montes E Alto Douro Escola De Ciencias Agrarias E Veterinarias Genet. Biotech. Animal Veterinary Res. CentreSpeech and Hearing Therapy Department São Paulo State University - UnespPrograma De Pós-Graduação Em Biologia Geral E Aplicada–São Paulo State University–Unesp Instituto De BiociênciasUniversidade Estadual Paulista (UNESP)Universidade Estadual de Campinas (UNICAMP)University of Trás-os-Montes and Alto Douro (Utad)Genet. Biotech. Animal Veterinary Res. CentreMaistro, Edson Luis [UNESP]Terrazzas, Peterson Menezes [UNESP]Sawaya, Alexandra Christinie Helena FranklandRosa, Paulo Cesar PiresPerazzo, Fábio Ferreirade Mascarenhas Gaivão, Isabel O’Neill2022-05-01T09:47:25Z2022-05-01T09:47:25Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article121-130http://dx.doi.org/10.1080/15287394.2021.1989351Journal of Toxicology and Environmental Health - Part A: Current Issues, v. 85, n. 3, p. 121-130, 2022.1087-26201528-7394http://hdl.handle.net/11449/23372410.1080/15287394.2021.19893512-s2.0-85117594164Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Toxicology and Environmental Health - Part A: Current Issuesinfo:eu-repo/semantics/openAccess2024-08-09T17:40:01Zoai:repositorio.unesp.br:11449/233724Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-09T17:40:01Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv In vivo toxicogenic potential of Salix alba (Salicaceae) bark extract
title In vivo toxicogenic potential of Salix alba (Salicaceae) bark extract
spellingShingle In vivo toxicogenic potential of Salix alba (Salicaceae) bark extract
Maistro, Edson Luis [UNESP]
Salicaceae genotoxicity
Salix comet assay
Salix micronucleus test
White willow
title_short In vivo toxicogenic potential of Salix alba (Salicaceae) bark extract
title_full In vivo toxicogenic potential of Salix alba (Salicaceae) bark extract
title_fullStr In vivo toxicogenic potential of Salix alba (Salicaceae) bark extract
title_full_unstemmed In vivo toxicogenic potential of Salix alba (Salicaceae) bark extract
title_sort In vivo toxicogenic potential of Salix alba (Salicaceae) bark extract
author Maistro, Edson Luis [UNESP]
author_facet Maistro, Edson Luis [UNESP]
Terrazzas, Peterson Menezes [UNESP]
Sawaya, Alexandra Christinie Helena Frankland
Rosa, Paulo Cesar Pires
Perazzo, Fábio Ferreira
de Mascarenhas Gaivão, Isabel O’Neill
author_role author
author2 Terrazzas, Peterson Menezes [UNESP]
Sawaya, Alexandra Christinie Helena Frankland
Rosa, Paulo Cesar Pires
Perazzo, Fábio Ferreira
de Mascarenhas Gaivão, Isabel O’Neill
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Estadual de Campinas (UNICAMP)
University of Trás-os-Montes and Alto Douro (Utad)
Genet. Biotech. Animal Veterinary Res. Centre
dc.contributor.author.fl_str_mv Maistro, Edson Luis [UNESP]
Terrazzas, Peterson Menezes [UNESP]
Sawaya, Alexandra Christinie Helena Frankland
Rosa, Paulo Cesar Pires
Perazzo, Fábio Ferreira
de Mascarenhas Gaivão, Isabel O’Neill
dc.subject.por.fl_str_mv Salicaceae genotoxicity
Salix comet assay
Salix micronucleus test
White willow
topic Salicaceae genotoxicity
Salix comet assay
Salix micronucleus test
White willow
description Salix alba (white willow) bark extract is widely used for conditions associated with inflammation, fever, microbial infection or pain. Exposure of human cultured leukocytes to S. alba in vitro noted a genotoxic response. However, data regarding the influence of this bark extract on DNA damage in vivo are lacking. The main goal of this study was to examine the potential of S.alba bark extract to induce DNA damage and chromosome aberrations in an in vivo model using cells obtained from male Swiss albino mice administered the compound orally. The extract was administered by oral gavage daily for 7 days at doses of 500, 1000, or 2000 mg/kg b.w. Genotoxicity analysis was performed using the comet assay on peripheral blood leukocytes, as well as liver, bone marrow, heart, and testicular cells collected 4 hr after the last treatment and the micronucleus (MN) test on bone marrow cells. In essence cells were collected 28 hr after the penultimate treatment Data demonstrated that S. alba bark extract did not induce significant DNA damage in any cell types examined, or clastogenic/aneugenic effects as detected by the MN test at the three tested doses. Under these experimental conditions, evidence indicates that S.alba bark extract did not initiate genotoxic or chromosome aberrations in various mouse cells investigated.
publishDate 2022
dc.date.none.fl_str_mv 2022-05-01T09:47:25Z
2022-05-01T09:47:25Z
2022-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1080/15287394.2021.1989351
Journal of Toxicology and Environmental Health - Part A: Current Issues, v. 85, n. 3, p. 121-130, 2022.
1087-2620
1528-7394
http://hdl.handle.net/11449/233724
10.1080/15287394.2021.1989351
2-s2.0-85117594164
url http://dx.doi.org/10.1080/15287394.2021.1989351
http://hdl.handle.net/11449/233724
identifier_str_mv Journal of Toxicology and Environmental Health - Part A: Current Issues, v. 85, n. 3, p. 121-130, 2022.
1087-2620
1528-7394
10.1080/15287394.2021.1989351
2-s2.0-85117594164
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Toxicology and Environmental Health - Part A: Current Issues
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 121-130
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128191510872064