Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applications
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1021/acsanm.2c02774 http://hdl.handle.net/11449/247726 |
Resumo: | Oxygen-generating biomaterials have the potential to improve tissue engineering and regenerative therapeutic strategies. However, the development of such materials capable of controlling the local partial pressure of oxygen (pO2) in the long term is still a major challenge. Here we report nanostructured composite membranes comprising electrospun fibers exhibiting a bead-on-string structure as a controlled oxygen-release system for periodontitis treatment. For this, calcium peroxide nanoparticles (CaO2NPs) and manganese dioxide nanosheets (MnO2NSs) were incorporated into the structure of hydrophobic electrospun poly (lactic acid) (PLA)-based nanofibers. We use CaO2NPs as hydrogen peroxide (H2O2)-generating precursors when exposed to water, while MnO2NSs were applied as a nanozyme to catalyze the decomposition of H2O2to the final oxygen product. Our results revealed that the beads on the fibrous structure acted as reservoirs of CaO2NPs and MnO2NSs. Moreover, the composite membranes provided sustained oxygen release over 7 days, where levels were modulated by the CaO2NP content. Such constructs exhibited suitable physicochemical properties and antimicrobial activities against some bacteria (e.g., Porphyromonas gingivalis and Treponema denticola) typically associated with aggressive and chronic periodontitis. In vitro studies also revealed that the membranes were not cytotoxic toward human oral keratinocyte (Nok-si) cells as well as enhanced the cell viability when high content of CaO2NP and MnO2NS were incorporated into the fiber's structure. Taken together, our results demonstrate that the nanostructured composite membranes show potential to be employed as oxygen-release platforms for periodontal tissue regeneration. |
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Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applicationsbead-on-string fiberscomposite membraneselectrospinningoxygen generationperiodontal regeneration, hybrid materialsOxygen-generating biomaterials have the potential to improve tissue engineering and regenerative therapeutic strategies. However, the development of such materials capable of controlling the local partial pressure of oxygen (pO2) in the long term is still a major challenge. Here we report nanostructured composite membranes comprising electrospun fibers exhibiting a bead-on-string structure as a controlled oxygen-release system for periodontitis treatment. For this, calcium peroxide nanoparticles (CaO2NPs) and manganese dioxide nanosheets (MnO2NSs) were incorporated into the structure of hydrophobic electrospun poly (lactic acid) (PLA)-based nanofibers. We use CaO2NPs as hydrogen peroxide (H2O2)-generating precursors when exposed to water, while MnO2NSs were applied as a nanozyme to catalyze the decomposition of H2O2to the final oxygen product. Our results revealed that the beads on the fibrous structure acted as reservoirs of CaO2NPs and MnO2NSs. Moreover, the composite membranes provided sustained oxygen release over 7 days, where levels were modulated by the CaO2NP content. Such constructs exhibited suitable physicochemical properties and antimicrobial activities against some bacteria (e.g., Porphyromonas gingivalis and Treponema denticola) typically associated with aggressive and chronic periodontitis. In vitro studies also revealed that the membranes were not cytotoxic toward human oral keratinocyte (Nok-si) cells as well as enhanced the cell viability when high content of CaO2NP and MnO2NS were incorporated into the fiber's structure. Taken together, our results demonstrate that the nanostructured composite membranes show potential to be employed as oxygen-release platforms for periodontal tissue regeneration.Nanotechnology National Laboratory for Agriculture (LNNA) Embrapa Instrumentação, São CarlosDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), Rua Humaitá, 1680-Centro, AraraquaraDepartment of Clinical Analysis School of Pharmaceutical Sciences São Paulo State University (UNESP), Rodovia Araraquara Jaú, Km 01-s/n-Campos Ville, AraraquaraSão Carlos Institute of Chemistry University of Sao Paulo, Avenue Trabalhador São-Carlense, 400, São PauloDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), Rua Humaitá, 1680-Centro, AraraquaraDepartment of Clinical Analysis School of Pharmaceutical Sciences São Paulo State University (UNESP), Rodovia Araraquara Jaú, Km 01-s/n-Campos Ville, AraraquaraEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Dos Santos, Danilo M.Dias, Luana M. [UNESP]Surur, Amanda K. [UNESP]De Moraes, Daniel A.Pavarina, Ana C. [UNESP]Fontana, Carla R. [UNESP]Correa, Daniel S.2023-07-29T13:24:09Z2023-07-29T13:24:09Z2022-10-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14425-14436http://dx.doi.org/10.1021/acsanm.2c02774ACS Applied Nano Materials, v. 5, n. 10, p. 14425-14436, 2022.2574-0970http://hdl.handle.net/11449/24772610.1021/acsanm.2c027742-s2.0-85139567450Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengACS Applied Nano Materialsinfo:eu-repo/semantics/openAccess2024-09-27T14:56:25Zoai:repositorio.unesp.br:11449/247726Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:56:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applications |
title |
Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applications |
spellingShingle |
Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applications Dos Santos, Danilo M. bead-on-string fibers composite membranes electrospinning oxygen generation periodontal regeneration, hybrid materials |
title_short |
Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applications |
title_full |
Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applications |
title_fullStr |
Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applications |
title_full_unstemmed |
Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applications |
title_sort |
Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applications |
author |
Dos Santos, Danilo M. |
author_facet |
Dos Santos, Danilo M. Dias, Luana M. [UNESP] Surur, Amanda K. [UNESP] De Moraes, Daniel A. Pavarina, Ana C. [UNESP] Fontana, Carla R. [UNESP] Correa, Daniel S. |
author_role |
author |
author2 |
Dias, Luana M. [UNESP] Surur, Amanda K. [UNESP] De Moraes, Daniel A. Pavarina, Ana C. [UNESP] Fontana, Carla R. [UNESP] Correa, Daniel S. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Dos Santos, Danilo M. Dias, Luana M. [UNESP] Surur, Amanda K. [UNESP] De Moraes, Daniel A. Pavarina, Ana C. [UNESP] Fontana, Carla R. [UNESP] Correa, Daniel S. |
dc.subject.por.fl_str_mv |
bead-on-string fibers composite membranes electrospinning oxygen generation periodontal regeneration, hybrid materials |
topic |
bead-on-string fibers composite membranes electrospinning oxygen generation periodontal regeneration, hybrid materials |
description |
Oxygen-generating biomaterials have the potential to improve tissue engineering and regenerative therapeutic strategies. However, the development of such materials capable of controlling the local partial pressure of oxygen (pO2) in the long term is still a major challenge. Here we report nanostructured composite membranes comprising electrospun fibers exhibiting a bead-on-string structure as a controlled oxygen-release system for periodontitis treatment. For this, calcium peroxide nanoparticles (CaO2NPs) and manganese dioxide nanosheets (MnO2NSs) were incorporated into the structure of hydrophobic electrospun poly (lactic acid) (PLA)-based nanofibers. We use CaO2NPs as hydrogen peroxide (H2O2)-generating precursors when exposed to water, while MnO2NSs were applied as a nanozyme to catalyze the decomposition of H2O2to the final oxygen product. Our results revealed that the beads on the fibrous structure acted as reservoirs of CaO2NPs and MnO2NSs. Moreover, the composite membranes provided sustained oxygen release over 7 days, where levels were modulated by the CaO2NP content. Such constructs exhibited suitable physicochemical properties and antimicrobial activities against some bacteria (e.g., Porphyromonas gingivalis and Treponema denticola) typically associated with aggressive and chronic periodontitis. In vitro studies also revealed that the membranes were not cytotoxic toward human oral keratinocyte (Nok-si) cells as well as enhanced the cell viability when high content of CaO2NP and MnO2NS were incorporated into the fiber's structure. Taken together, our results demonstrate that the nanostructured composite membranes show potential to be employed as oxygen-release platforms for periodontal tissue regeneration. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-28 2023-07-29T13:24:09Z 2023-07-29T13:24:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1021/acsanm.2c02774 ACS Applied Nano Materials, v. 5, n. 10, p. 14425-14436, 2022. 2574-0970 http://hdl.handle.net/11449/247726 10.1021/acsanm.2c02774 2-s2.0-85139567450 |
url |
http://dx.doi.org/10.1021/acsanm.2c02774 http://hdl.handle.net/11449/247726 |
identifier_str_mv |
ACS Applied Nano Materials, v. 5, n. 10, p. 14425-14436, 2022. 2574-0970 10.1021/acsanm.2c02774 2-s2.0-85139567450 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
ACS Applied Nano Materials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14425-14436 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546386367447040 |