Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applications

Detalhes bibliográficos
Autor(a) principal: Dos Santos, Danilo M.
Data de Publicação: 2022
Outros Autores: Dias, Luana M. [UNESP], Surur, Amanda K. [UNESP], De Moraes, Daniel A., Pavarina, Ana C. [UNESP], Fontana, Carla R. [UNESP], Correa, Daniel S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1021/acsanm.2c02774
http://hdl.handle.net/11449/247726
Resumo: Oxygen-generating biomaterials have the potential to improve tissue engineering and regenerative therapeutic strategies. However, the development of such materials capable of controlling the local partial pressure of oxygen (pO2) in the long term is still a major challenge. Here we report nanostructured composite membranes comprising electrospun fibers exhibiting a bead-on-string structure as a controlled oxygen-release system for periodontitis treatment. For this, calcium peroxide nanoparticles (CaO2NPs) and manganese dioxide nanosheets (MnO2NSs) were incorporated into the structure of hydrophobic electrospun poly (lactic acid) (PLA)-based nanofibers. We use CaO2NPs as hydrogen peroxide (H2O2)-generating precursors when exposed to water, while MnO2NSs were applied as a nanozyme to catalyze the decomposition of H2O2to the final oxygen product. Our results revealed that the beads on the fibrous structure acted as reservoirs of CaO2NPs and MnO2NSs. Moreover, the composite membranes provided sustained oxygen release over 7 days, where levels were modulated by the CaO2NP content. Such constructs exhibited suitable physicochemical properties and antimicrobial activities against some bacteria (e.g., Porphyromonas gingivalis and Treponema denticola) typically associated with aggressive and chronic periodontitis. In vitro studies also revealed that the membranes were not cytotoxic toward human oral keratinocyte (Nok-si) cells as well as enhanced the cell viability when high content of CaO2NP and MnO2NS were incorporated into the fiber's structure. Taken together, our results demonstrate that the nanostructured composite membranes show potential to be employed as oxygen-release platforms for periodontal tissue regeneration.
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spelling Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applicationsbead-on-string fiberscomposite membraneselectrospinningoxygen generationperiodontal regeneration, hybrid materialsOxygen-generating biomaterials have the potential to improve tissue engineering and regenerative therapeutic strategies. However, the development of such materials capable of controlling the local partial pressure of oxygen (pO2) in the long term is still a major challenge. Here we report nanostructured composite membranes comprising electrospun fibers exhibiting a bead-on-string structure as a controlled oxygen-release system for periodontitis treatment. For this, calcium peroxide nanoparticles (CaO2NPs) and manganese dioxide nanosheets (MnO2NSs) were incorporated into the structure of hydrophobic electrospun poly (lactic acid) (PLA)-based nanofibers. We use CaO2NPs as hydrogen peroxide (H2O2)-generating precursors when exposed to water, while MnO2NSs were applied as a nanozyme to catalyze the decomposition of H2O2to the final oxygen product. Our results revealed that the beads on the fibrous structure acted as reservoirs of CaO2NPs and MnO2NSs. Moreover, the composite membranes provided sustained oxygen release over 7 days, where levels were modulated by the CaO2NP content. Such constructs exhibited suitable physicochemical properties and antimicrobial activities against some bacteria (e.g., Porphyromonas gingivalis and Treponema denticola) typically associated with aggressive and chronic periodontitis. In vitro studies also revealed that the membranes were not cytotoxic toward human oral keratinocyte (Nok-si) cells as well as enhanced the cell viability when high content of CaO2NP and MnO2NS were incorporated into the fiber's structure. Taken together, our results demonstrate that the nanostructured composite membranes show potential to be employed as oxygen-release platforms for periodontal tissue regeneration.Nanotechnology National Laboratory for Agriculture (LNNA) Embrapa Instrumentação, São CarlosDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), Rua Humaitá, 1680-Centro, AraraquaraDepartment of Clinical Analysis School of Pharmaceutical Sciences São Paulo State University (UNESP), Rodovia Araraquara Jaú, Km 01-s/n-Campos Ville, AraraquaraSão Carlos Institute of Chemistry University of Sao Paulo, Avenue Trabalhador São-Carlense, 400, São PauloDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), Rua Humaitá, 1680-Centro, AraraquaraDepartment of Clinical Analysis School of Pharmaceutical Sciences São Paulo State University (UNESP), Rodovia Araraquara Jaú, Km 01-s/n-Campos Ville, AraraquaraEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Dos Santos, Danilo M.Dias, Luana M. [UNESP]Surur, Amanda K. [UNESP]De Moraes, Daniel A.Pavarina, Ana C. [UNESP]Fontana, Carla R. [UNESP]Correa, Daniel S.2023-07-29T13:24:09Z2023-07-29T13:24:09Z2022-10-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14425-14436http://dx.doi.org/10.1021/acsanm.2c02774ACS Applied Nano Materials, v. 5, n. 10, p. 14425-14436, 2022.2574-0970http://hdl.handle.net/11449/24772610.1021/acsanm.2c027742-s2.0-85139567450Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengACS Applied Nano Materialsinfo:eu-repo/semantics/openAccess2024-09-27T14:56:25Zoai:repositorio.unesp.br:11449/247726Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:56:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applications
title Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applications
spellingShingle Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applications
Dos Santos, Danilo M.
bead-on-string fibers
composite membranes
electrospinning
oxygen generation
periodontal regeneration, hybrid materials
title_short Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applications
title_full Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applications
title_fullStr Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applications
title_full_unstemmed Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applications
title_sort Electrospun Composite Bead-on-String Nanofibers Containing CaO2Nanoparticles and MnO2Nanosheets as Oxygen-Release Systems for Biomedical Applications
author Dos Santos, Danilo M.
author_facet Dos Santos, Danilo M.
Dias, Luana M. [UNESP]
Surur, Amanda K. [UNESP]
De Moraes, Daniel A.
Pavarina, Ana C. [UNESP]
Fontana, Carla R. [UNESP]
Correa, Daniel S.
author_role author
author2 Dias, Luana M. [UNESP]
Surur, Amanda K. [UNESP]
De Moraes, Daniel A.
Pavarina, Ana C. [UNESP]
Fontana, Carla R. [UNESP]
Correa, Daniel S.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade Estadual Paulista (UNESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Dos Santos, Danilo M.
Dias, Luana M. [UNESP]
Surur, Amanda K. [UNESP]
De Moraes, Daniel A.
Pavarina, Ana C. [UNESP]
Fontana, Carla R. [UNESP]
Correa, Daniel S.
dc.subject.por.fl_str_mv bead-on-string fibers
composite membranes
electrospinning
oxygen generation
periodontal regeneration, hybrid materials
topic bead-on-string fibers
composite membranes
electrospinning
oxygen generation
periodontal regeneration, hybrid materials
description Oxygen-generating biomaterials have the potential to improve tissue engineering and regenerative therapeutic strategies. However, the development of such materials capable of controlling the local partial pressure of oxygen (pO2) in the long term is still a major challenge. Here we report nanostructured composite membranes comprising electrospun fibers exhibiting a bead-on-string structure as a controlled oxygen-release system for periodontitis treatment. For this, calcium peroxide nanoparticles (CaO2NPs) and manganese dioxide nanosheets (MnO2NSs) were incorporated into the structure of hydrophobic electrospun poly (lactic acid) (PLA)-based nanofibers. We use CaO2NPs as hydrogen peroxide (H2O2)-generating precursors when exposed to water, while MnO2NSs were applied as a nanozyme to catalyze the decomposition of H2O2to the final oxygen product. Our results revealed that the beads on the fibrous structure acted as reservoirs of CaO2NPs and MnO2NSs. Moreover, the composite membranes provided sustained oxygen release over 7 days, where levels were modulated by the CaO2NP content. Such constructs exhibited suitable physicochemical properties and antimicrobial activities against some bacteria (e.g., Porphyromonas gingivalis and Treponema denticola) typically associated with aggressive and chronic periodontitis. In vitro studies also revealed that the membranes were not cytotoxic toward human oral keratinocyte (Nok-si) cells as well as enhanced the cell viability when high content of CaO2NP and MnO2NS were incorporated into the fiber's structure. Taken together, our results demonstrate that the nanostructured composite membranes show potential to be employed as oxygen-release platforms for periodontal tissue regeneration.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-28
2023-07-29T13:24:09Z
2023-07-29T13:24:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1021/acsanm.2c02774
ACS Applied Nano Materials, v. 5, n. 10, p. 14425-14436, 2022.
2574-0970
http://hdl.handle.net/11449/247726
10.1021/acsanm.2c02774
2-s2.0-85139567450
url http://dx.doi.org/10.1021/acsanm.2c02774
http://hdl.handle.net/11449/247726
identifier_str_mv ACS Applied Nano Materials, v. 5, n. 10, p. 14425-14436, 2022.
2574-0970
10.1021/acsanm.2c02774
2-s2.0-85139567450
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv ACS Applied Nano Materials
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14425-14436
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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