Modulation of hypercapnic respiratory response by cholinergic transmission in the commissural nucleus of the solitary tract
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00424-019-02341-9 http://hdl.handle.net/11449/201433 |
Resumo: | The nucleus of the solitary tract (NTS) is an important area of the brainstem that receives and integrates afferent cardiorespiratory sensorial information, including those from arterial chemoreceptors and baroreceptors. It was described that acetylcholine (ACh) in the commissural subnucleus of the NTS (cNTS) promotes an increase in the phrenic nerve activity (PNA) and antagonism of nicotinic receptors in the same region reduces the magnitude of tachypneic response to peripheral chemoreceptor stimulation, suggesting a functional role of cholinergic transmission within the cNTS in the chemosensory control of respiratory activity. In the present study, we investigated whether cholinergic receptor antagonism in the cNTS modifies the sympathetic and respiratory reflex responses to hypercapnia. Using an arterially perfused in situ preparation of juvenile male Holtzman rats, we found that the nicotinic antagonist (mecamylamine, 5 mM), but not the muscarinic antagonist (atropine, 5 mM), into the cNTS attenuated the hypercapnia-induced increase of hypoglossal activity. Furthermore, mecamylamine in the cNTS potentiated the generation of late-expiratory (late-E) activity in abdominal nerve induced by hypercapnia. None of the cholinergic antagonists microinjected in the cNTS changed either the sympathetic or the phrenic nerve responses to hypercapnia. Our data provide evidence for the role of cholinergic transmission in the cNTS, acting on nicotinic receptors, modulating the hypoglossal and abdominal responses to hypercapnia. |
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Modulation of hypercapnic respiratory response by cholinergic transmission in the commissural nucleus of the solitary tractAcetylcholineBrainstemChemoreflexHypercapniaMuscarinic receptorsNicotinic receptorsThe nucleus of the solitary tract (NTS) is an important area of the brainstem that receives and integrates afferent cardiorespiratory sensorial information, including those from arterial chemoreceptors and baroreceptors. It was described that acetylcholine (ACh) in the commissural subnucleus of the NTS (cNTS) promotes an increase in the phrenic nerve activity (PNA) and antagonism of nicotinic receptors in the same region reduces the magnitude of tachypneic response to peripheral chemoreceptor stimulation, suggesting a functional role of cholinergic transmission within the cNTS in the chemosensory control of respiratory activity. In the present study, we investigated whether cholinergic receptor antagonism in the cNTS modifies the sympathetic and respiratory reflex responses to hypercapnia. Using an arterially perfused in situ preparation of juvenile male Holtzman rats, we found that the nicotinic antagonist (mecamylamine, 5 mM), but not the muscarinic antagonist (atropine, 5 mM), into the cNTS attenuated the hypercapnia-induced increase of hypoglossal activity. Furthermore, mecamylamine in the cNTS potentiated the generation of late-expiratory (late-E) activity in abdominal nerve induced by hypercapnia. None of the cholinergic antagonists microinjected in the cNTS changed either the sympathetic or the phrenic nerve responses to hypercapnia. Our data provide evidence for the role of cholinergic transmission in the cNTS, acting on nicotinic receptors, modulating the hypoglossal and abdominal responses to hypercapnia.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Physiology and Pathology School of Dentistry UNESP - São Paulo State UniversityDepartment of Physiology and Pathology School of Dentistry UNESP - São Paulo State UniversityFAPESP: 2013/17251-6FAPESP: 2013/22526-4FAPESP: 2015/234677CNPq: 304873/2014-4CNPq: 310331/2017-0CNPq: 408950/2018-8CNPq: 425586/2016-2CAPES: Finance Code 001Universidade Estadual Paulista (Unesp)Furuya, Werner I. [UNESP]Bassi, Mirian [UNESP]Menani, José V. [UNESP]Colombari, Eduardo [UNESP]Zoccal, Daniel B. [UNESP]Colombari, Débora S. A. [UNESP]2020-12-12T02:32:21Z2020-12-12T02:32:21Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article49-60http://dx.doi.org/10.1007/s00424-019-02341-9Pflugers Archiv European Journal of Physiology, v. 472, n. 1, p. 49-60, 2020.1432-20130031-6768http://hdl.handle.net/11449/20143310.1007/s00424-019-02341-92-s2.0-85077158625Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPflugers Archiv European Journal of Physiologyinfo:eu-repo/semantics/openAccess2021-10-22T19:03:36Zoai:repositorio.unesp.br:11449/201433Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T19:03:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Modulation of hypercapnic respiratory response by cholinergic transmission in the commissural nucleus of the solitary tract |
title |
Modulation of hypercapnic respiratory response by cholinergic transmission in the commissural nucleus of the solitary tract |
spellingShingle |
Modulation of hypercapnic respiratory response by cholinergic transmission in the commissural nucleus of the solitary tract Furuya, Werner I. [UNESP] Acetylcholine Brainstem Chemoreflex Hypercapnia Muscarinic receptors Nicotinic receptors |
title_short |
Modulation of hypercapnic respiratory response by cholinergic transmission in the commissural nucleus of the solitary tract |
title_full |
Modulation of hypercapnic respiratory response by cholinergic transmission in the commissural nucleus of the solitary tract |
title_fullStr |
Modulation of hypercapnic respiratory response by cholinergic transmission in the commissural nucleus of the solitary tract |
title_full_unstemmed |
Modulation of hypercapnic respiratory response by cholinergic transmission in the commissural nucleus of the solitary tract |
title_sort |
Modulation of hypercapnic respiratory response by cholinergic transmission in the commissural nucleus of the solitary tract |
author |
Furuya, Werner I. [UNESP] |
author_facet |
Furuya, Werner I. [UNESP] Bassi, Mirian [UNESP] Menani, José V. [UNESP] Colombari, Eduardo [UNESP] Zoccal, Daniel B. [UNESP] Colombari, Débora S. A. [UNESP] |
author_role |
author |
author2 |
Bassi, Mirian [UNESP] Menani, José V. [UNESP] Colombari, Eduardo [UNESP] Zoccal, Daniel B. [UNESP] Colombari, Débora S. A. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Furuya, Werner I. [UNESP] Bassi, Mirian [UNESP] Menani, José V. [UNESP] Colombari, Eduardo [UNESP] Zoccal, Daniel B. [UNESP] Colombari, Débora S. A. [UNESP] |
dc.subject.por.fl_str_mv |
Acetylcholine Brainstem Chemoreflex Hypercapnia Muscarinic receptors Nicotinic receptors |
topic |
Acetylcholine Brainstem Chemoreflex Hypercapnia Muscarinic receptors Nicotinic receptors |
description |
The nucleus of the solitary tract (NTS) is an important area of the brainstem that receives and integrates afferent cardiorespiratory sensorial information, including those from arterial chemoreceptors and baroreceptors. It was described that acetylcholine (ACh) in the commissural subnucleus of the NTS (cNTS) promotes an increase in the phrenic nerve activity (PNA) and antagonism of nicotinic receptors in the same region reduces the magnitude of tachypneic response to peripheral chemoreceptor stimulation, suggesting a functional role of cholinergic transmission within the cNTS in the chemosensory control of respiratory activity. In the present study, we investigated whether cholinergic receptor antagonism in the cNTS modifies the sympathetic and respiratory reflex responses to hypercapnia. Using an arterially perfused in situ preparation of juvenile male Holtzman rats, we found that the nicotinic antagonist (mecamylamine, 5 mM), but not the muscarinic antagonist (atropine, 5 mM), into the cNTS attenuated the hypercapnia-induced increase of hypoglossal activity. Furthermore, mecamylamine in the cNTS potentiated the generation of late-expiratory (late-E) activity in abdominal nerve induced by hypercapnia. None of the cholinergic antagonists microinjected in the cNTS changed either the sympathetic or the phrenic nerve responses to hypercapnia. Our data provide evidence for the role of cholinergic transmission in the cNTS, acting on nicotinic receptors, modulating the hypoglossal and abdominal responses to hypercapnia. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:32:21Z 2020-12-12T02:32:21Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00424-019-02341-9 Pflugers Archiv European Journal of Physiology, v. 472, n. 1, p. 49-60, 2020. 1432-2013 0031-6768 http://hdl.handle.net/11449/201433 10.1007/s00424-019-02341-9 2-s2.0-85077158625 |
url |
http://dx.doi.org/10.1007/s00424-019-02341-9 http://hdl.handle.net/11449/201433 |
identifier_str_mv |
Pflugers Archiv European Journal of Physiology, v. 472, n. 1, p. 49-60, 2020. 1432-2013 0031-6768 10.1007/s00424-019-02341-9 2-s2.0-85077158625 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pflugers Archiv European Journal of Physiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
49-60 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1797790227763298304 |