A metodologia de superfície de resposta como ferramenta para a avaliação da produção de alginato e poli-hidroxibutirato pela Azotobacter vinelandii
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | |
Tipo de documento: | Artigo de conferência |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4025/actascitechnol.v32i2.1792 http://hdl.handle.net/11449/42540 |
Resumo: | The response surface methodology as a tool for assessing the production of alginate and polyhydroxybutirate by Azotobacter vinelandii. Alginate is a polysaccharide extracted from cell walls of brown algae and used in the food, pharmaceuticals and biotech industries. Production is concentrated on the cultivation of brown seaweed, but several bacteria of the genus Pseudomonas and Azotobacter produce alginate. The chemical structure of alginates produced by algae is similar to those synthesized by A. vinelandii. The bacteria also produce intracellular polymers such as polyhydroxybutyrate (PHB), known as bioplastic. This work studied the simultaneous alginate and PHB production by A. vinelandii using sucrose and different parameters of fermentation in an orbital shaker. The optimal values for the production of these compounds were determined by the MSR. The first experiment was a 2(6-2) factorial design. The second was based on significant variables of the first, resulting in a full 3(3-0) factorial design. From the first to the second, an increase was observed in the PHB productivity from 12 to 45 mg g(-1) cell h(-1) and alginate from 100 to 1,600 mg g(-1) of cell h(-1). The productivity of both compounds was in the maximum incubation temperature of 62 degrees C, in the shortest time of incubation (18h) and the sucrose concentration, 11 g L(-1). In both experiments the PHB extracted presented purity of 94%. |
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A metodologia de superfície de resposta como ferramenta para a avaliação da produção de alginato e poli-hidroxibutirato pela Azotobacter vinelandiiThe response surface methodology as a tool for assessing the production of alginate and polyhydroxybutirate by Azotobacter vinelandiialginatepolyhydroxybutyrate (PHB)productionAzotobacter vinelandiiThe response surface methodology as a tool for assessing the production of alginate and polyhydroxybutirate by Azotobacter vinelandii. Alginate is a polysaccharide extracted from cell walls of brown algae and used in the food, pharmaceuticals and biotech industries. Production is concentrated on the cultivation of brown seaweed, but several bacteria of the genus Pseudomonas and Azotobacter produce alginate. The chemical structure of alginates produced by algae is similar to those synthesized by A. vinelandii. The bacteria also produce intracellular polymers such as polyhydroxybutyrate (PHB), known as bioplastic. This work studied the simultaneous alginate and PHB production by A. vinelandii using sucrose and different parameters of fermentation in an orbital shaker. The optimal values for the production of these compounds were determined by the MSR. The first experiment was a 2(6-2) factorial design. The second was based on significant variables of the first, resulting in a full 3(3-0) factorial design. From the first to the second, an increase was observed in the PHB productivity from 12 to 45 mg g(-1) cell h(-1) and alginate from 100 to 1,600 mg g(-1) of cell h(-1). The productivity of both compounds was in the maximum incubation temperature of 62 degrees C, in the shortest time of incubation (18h) and the sucrose concentration, 11 g L(-1). In both experiments the PHB extracted presented purity of 94%.Univ Estadual Paulista, BR-15054000 São Paulo, BrazilUniv Estadual Paulista, BR-15054000 São Paulo, BrazilUniversidade Estadual de Maringá (UEM), Pro-reitoria Pesquisa Pos-graduacaoUniversidade Estadual Paulista (Unesp)Silva, Adriana Navarro da [UNESP]Garcia-Cruz, Crispin Humberto [UNESP]2014-05-20T15:34:24Z2014-05-20T15:34:24Z2010-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject105-112application/pdfhttp://dx.doi.org/10.4025/actascitechnol.v32i2.1792Acta Scientiarum-technology. Maringa: Universidade Estadual de Maringá (UEM), Pro-reitoria Pesquisa Pos-graduacao, v. 32, n. 2, p. 105-112, 2010.1806-2563http://hdl.handle.net/11449/4254010.4025/actascitechnol.v32i2.1792WOS:000282011100001WOS000282011100001.pdf3761109299906668Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporActa Scientiarum: Technology0.2310,168info:eu-repo/semantics/openAccess2023-10-04T06:01:38Zoai:repositorio.unesp.br:11449/42540Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:57:04.390155Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A metodologia de superfície de resposta como ferramenta para a avaliação da produção de alginato e poli-hidroxibutirato pela Azotobacter vinelandii The response surface methodology as a tool for assessing the production of alginate and polyhydroxybutirate by Azotobacter vinelandii |
title |
A metodologia de superfície de resposta como ferramenta para a avaliação da produção de alginato e poli-hidroxibutirato pela Azotobacter vinelandii |
spellingShingle |
A metodologia de superfície de resposta como ferramenta para a avaliação da produção de alginato e poli-hidroxibutirato pela Azotobacter vinelandii Silva, Adriana Navarro da [UNESP] alginate polyhydroxybutyrate (PHB) production Azotobacter vinelandii |
title_short |
A metodologia de superfície de resposta como ferramenta para a avaliação da produção de alginato e poli-hidroxibutirato pela Azotobacter vinelandii |
title_full |
A metodologia de superfície de resposta como ferramenta para a avaliação da produção de alginato e poli-hidroxibutirato pela Azotobacter vinelandii |
title_fullStr |
A metodologia de superfície de resposta como ferramenta para a avaliação da produção de alginato e poli-hidroxibutirato pela Azotobacter vinelandii |
title_full_unstemmed |
A metodologia de superfície de resposta como ferramenta para a avaliação da produção de alginato e poli-hidroxibutirato pela Azotobacter vinelandii |
title_sort |
A metodologia de superfície de resposta como ferramenta para a avaliação da produção de alginato e poli-hidroxibutirato pela Azotobacter vinelandii |
author |
Silva, Adriana Navarro da [UNESP] |
author_facet |
Silva, Adriana Navarro da [UNESP] Garcia-Cruz, Crispin Humberto [UNESP] |
author_role |
author |
author2 |
Garcia-Cruz, Crispin Humberto [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silva, Adriana Navarro da [UNESP] Garcia-Cruz, Crispin Humberto [UNESP] |
dc.subject.por.fl_str_mv |
alginate polyhydroxybutyrate (PHB) production Azotobacter vinelandii |
topic |
alginate polyhydroxybutyrate (PHB) production Azotobacter vinelandii |
description |
The response surface methodology as a tool for assessing the production of alginate and polyhydroxybutirate by Azotobacter vinelandii. Alginate is a polysaccharide extracted from cell walls of brown algae and used in the food, pharmaceuticals and biotech industries. Production is concentrated on the cultivation of brown seaweed, but several bacteria of the genus Pseudomonas and Azotobacter produce alginate. The chemical structure of alginates produced by algae is similar to those synthesized by A. vinelandii. The bacteria also produce intracellular polymers such as polyhydroxybutyrate (PHB), known as bioplastic. This work studied the simultaneous alginate and PHB production by A. vinelandii using sucrose and different parameters of fermentation in an orbital shaker. The optimal values for the production of these compounds were determined by the MSR. The first experiment was a 2(6-2) factorial design. The second was based on significant variables of the first, resulting in a full 3(3-0) factorial design. From the first to the second, an increase was observed in the PHB productivity from 12 to 45 mg g(-1) cell h(-1) and alginate from 100 to 1,600 mg g(-1) of cell h(-1). The productivity of both compounds was in the maximum incubation temperature of 62 degrees C, in the shortest time of incubation (18h) and the sucrose concentration, 11 g L(-1). In both experiments the PHB extracted presented purity of 94%. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 2014-05-20T15:34:24Z 2014-05-20T15:34:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4025/actascitechnol.v32i2.1792 Acta Scientiarum-technology. Maringa: Universidade Estadual de Maringá (UEM), Pro-reitoria Pesquisa Pos-graduacao, v. 32, n. 2, p. 105-112, 2010. 1806-2563 http://hdl.handle.net/11449/42540 10.4025/actascitechnol.v32i2.1792 WOS:000282011100001 WOS000282011100001.pdf 3761109299906668 |
url |
http://dx.doi.org/10.4025/actascitechnol.v32i2.1792 http://hdl.handle.net/11449/42540 |
identifier_str_mv |
Acta Scientiarum-technology. Maringa: Universidade Estadual de Maringá (UEM), Pro-reitoria Pesquisa Pos-graduacao, v. 32, n. 2, p. 105-112, 2010. 1806-2563 10.4025/actascitechnol.v32i2.1792 WOS:000282011100001 WOS000282011100001.pdf 3761109299906668 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Acta Scientiarum: Technology 0.231 0,168 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
105-112 application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá (UEM), Pro-reitoria Pesquisa Pos-graduacao |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá (UEM), Pro-reitoria Pesquisa Pos-graduacao |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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