Identification of Mycobacterium species and Rhodococcus equi in peccary lymph nodes

Detalhes bibliográficos
Autor(a) principal: de Morais, Amanda Bonalume Cordeiro [UNESP]
Data de Publicação: 2018
Outros Autores: Bolaños, Carmen Alicia Daza [UNESP], Alves, Ana Carolina [UNESP], Ikuta, Cássia Yumi, Lara, Gustavo Henrique Batista [UNESP], Heinemann, Marcos Bryan, Giuffrida, Rogério, Listoni, Fernando Paganini [UNESP], de Souza Ribeiro Mioni, Mateus [UNESP], Motta, Rodrigo Garcia, Takai, Shinji, Ribeiro, Márcio Garcia [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s11250-018-1562-2
http://hdl.handle.net/11449/176018
Resumo: Mycobacterium species and the virulence-associated proteins (vapA, vapB, and vapN genes) of Rhodococcus equi isolated from 330 lymph nodes of collared peccaries (Tayassu tajacu) and white-lipped peccaries (Tayassu pecari) intended for human consumption were investigated. Thirty-six (10.9%) R. equi strains were isolated; 3.3% (n = 11/330) were from white-lipped peccary lymph nodes, and 7.6% (25/330) were from collared peccary lymph nodes. Among the 11 isolates of R. equi from the white-lipped peccaries, 90.9% (n = 10/11) were obtained from the mesenteric lymph nodes, and only 9.1% (n = 1/10) were obtained from the mediastinal lymph nodes. In the 25 isolates of R. equi obtained from the collared peccaries, 40.0% (n = 10/25) were recovered from the mesenteric lymph nodes, 36% (n = 9/25) from the submandibular lymph nodes, and 24.0% (n = 6/25) from the mediastinal lymph nodes. No vapA, vapB, or vapN genes (plasmidless) or three host-associated types (pVAPA, pVAPB, and pVAPN) were identified among the R. equi isolates. Mycobacterium species were isolated in 3.03% (n = 10/330) of all the lymph nodes analyzed. Among the 10 mycobacterial isolates, 60% (n = 6/10) were from the white-lipped peccary lymph nodes, and 40% (n = 4/10) were from the collared peccary lymph nodes. Ten Mycobacterium species were detected by PCR-PRA with a predominance of M. avium type 1. Sequencing of the hsp65 and rpob genes revealed mycobacteria that were saprophytic (M. sinense and M. kumamotonense) and potentially pathogenic (M. colombiense and M. intracellulare) to humans and animals. To our knowledge, this is the first description of R. equi and/or mycobacterial species identified in the lymph nodes of peccary specimens. R. equi (plasmidless) and the mycobacterial species described here have been reported as causes of pulmonary and extrapulmonary infections in both immunocompetent and immunocompromised humans.
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spelling Identification of Mycobacterium species and Rhodococcus equi in peccary lymph nodesCollared peccaryMycobacteriaRhodococcosisTayassuidae speciesWhite-lipped peccaryMycobacterium species and the virulence-associated proteins (vapA, vapB, and vapN genes) of Rhodococcus equi isolated from 330 lymph nodes of collared peccaries (Tayassu tajacu) and white-lipped peccaries (Tayassu pecari) intended for human consumption were investigated. Thirty-six (10.9%) R. equi strains were isolated; 3.3% (n = 11/330) were from white-lipped peccary lymph nodes, and 7.6% (25/330) were from collared peccary lymph nodes. Among the 11 isolates of R. equi from the white-lipped peccaries, 90.9% (n = 10/11) were obtained from the mesenteric lymph nodes, and only 9.1% (n = 1/10) were obtained from the mediastinal lymph nodes. In the 25 isolates of R. equi obtained from the collared peccaries, 40.0% (n = 10/25) were recovered from the mesenteric lymph nodes, 36% (n = 9/25) from the submandibular lymph nodes, and 24.0% (n = 6/25) from the mediastinal lymph nodes. No vapA, vapB, or vapN genes (plasmidless) or three host-associated types (pVAPA, pVAPB, and pVAPN) were identified among the R. equi isolates. Mycobacterium species were isolated in 3.03% (n = 10/330) of all the lymph nodes analyzed. Among the 10 mycobacterial isolates, 60% (n = 6/10) were from the white-lipped peccary lymph nodes, and 40% (n = 4/10) were from the collared peccary lymph nodes. Ten Mycobacterium species were detected by PCR-PRA with a predominance of M. avium type 1. Sequencing of the hsp65 and rpob genes revealed mycobacteria that were saprophytic (M. sinense and M. kumamotonense) and potentially pathogenic (M. colombiense and M. intracellulare) to humans and animals. To our knowledge, this is the first description of R. equi and/or mycobacterial species identified in the lymph nodes of peccary specimens. R. equi (plasmidless) and the mycobacterial species described here have been reported as causes of pulmonary and extrapulmonary infections in both immunocompetent and immunocompromised humans.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences UNESP—São Paulo State UniversityDepartment of Preventive Veterinary Medicine and Animal Health University of São Paulo—USPSchool of Veterinary Medicine UNOESTE—University of Oeste PaulistaUniRV—University of Rio VerdeDepartment of Animal Hygiene School of Veterinary Medicine and Animal Sciences Kitasato UniversityDepartment of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences UNESP—São Paulo State UniversityUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)UNOESTE—University of Oeste PaulistaUniRV—University of Rio VerdeKitasato Universityde Morais, Amanda Bonalume Cordeiro [UNESP]Bolaños, Carmen Alicia Daza [UNESP]Alves, Ana Carolina [UNESP]Ikuta, Cássia YumiLara, Gustavo Henrique Batista [UNESP]Heinemann, Marcos BryanGiuffrida, RogérioListoni, Fernando Paganini [UNESP]de Souza Ribeiro Mioni, Mateus [UNESP]Motta, Rodrigo GarciaTakai, ShinjiRibeiro, Márcio Garcia [UNESP]2018-12-11T17:18:35Z2018-12-11T17:18:35Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1319-1326application/pdfhttp://dx.doi.org/10.1007/s11250-018-1562-2Tropical Animal Health and Production, v. 50, n. 6, p. 1319-1326, 2018.1573-74380049-4747http://hdl.handle.net/11449/17601810.1007/s11250-018-1562-22-s2.0-850440904512-s2.0-85044090451.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTropical Animal Health and Production0,5110,511info:eu-repo/semantics/openAccess2024-01-20T06:32:22Zoai:repositorio.unesp.br:11449/176018Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-20T06:32:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Identification of Mycobacterium species and Rhodococcus equi in peccary lymph nodes
title Identification of Mycobacterium species and Rhodococcus equi in peccary lymph nodes
spellingShingle Identification of Mycobacterium species and Rhodococcus equi in peccary lymph nodes
de Morais, Amanda Bonalume Cordeiro [UNESP]
Collared peccary
Mycobacteria
Rhodococcosis
Tayassuidae species
White-lipped peccary
title_short Identification of Mycobacterium species and Rhodococcus equi in peccary lymph nodes
title_full Identification of Mycobacterium species and Rhodococcus equi in peccary lymph nodes
title_fullStr Identification of Mycobacterium species and Rhodococcus equi in peccary lymph nodes
title_full_unstemmed Identification of Mycobacterium species and Rhodococcus equi in peccary lymph nodes
title_sort Identification of Mycobacterium species and Rhodococcus equi in peccary lymph nodes
author de Morais, Amanda Bonalume Cordeiro [UNESP]
author_facet de Morais, Amanda Bonalume Cordeiro [UNESP]
Bolaños, Carmen Alicia Daza [UNESP]
Alves, Ana Carolina [UNESP]
Ikuta, Cássia Yumi
Lara, Gustavo Henrique Batista [UNESP]
Heinemann, Marcos Bryan
Giuffrida, Rogério
Listoni, Fernando Paganini [UNESP]
de Souza Ribeiro Mioni, Mateus [UNESP]
Motta, Rodrigo Garcia
Takai, Shinji
Ribeiro, Márcio Garcia [UNESP]
author_role author
author2 Bolaños, Carmen Alicia Daza [UNESP]
Alves, Ana Carolina [UNESP]
Ikuta, Cássia Yumi
Lara, Gustavo Henrique Batista [UNESP]
Heinemann, Marcos Bryan
Giuffrida, Rogério
Listoni, Fernando Paganini [UNESP]
de Souza Ribeiro Mioni, Mateus [UNESP]
Motta, Rodrigo Garcia
Takai, Shinji
Ribeiro, Márcio Garcia [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
UNOESTE—University of Oeste Paulista
UniRV—University of Rio Verde
Kitasato University
dc.contributor.author.fl_str_mv de Morais, Amanda Bonalume Cordeiro [UNESP]
Bolaños, Carmen Alicia Daza [UNESP]
Alves, Ana Carolina [UNESP]
Ikuta, Cássia Yumi
Lara, Gustavo Henrique Batista [UNESP]
Heinemann, Marcos Bryan
Giuffrida, Rogério
Listoni, Fernando Paganini [UNESP]
de Souza Ribeiro Mioni, Mateus [UNESP]
Motta, Rodrigo Garcia
Takai, Shinji
Ribeiro, Márcio Garcia [UNESP]
dc.subject.por.fl_str_mv Collared peccary
Mycobacteria
Rhodococcosis
Tayassuidae species
White-lipped peccary
topic Collared peccary
Mycobacteria
Rhodococcosis
Tayassuidae species
White-lipped peccary
description Mycobacterium species and the virulence-associated proteins (vapA, vapB, and vapN genes) of Rhodococcus equi isolated from 330 lymph nodes of collared peccaries (Tayassu tajacu) and white-lipped peccaries (Tayassu pecari) intended for human consumption were investigated. Thirty-six (10.9%) R. equi strains were isolated; 3.3% (n = 11/330) were from white-lipped peccary lymph nodes, and 7.6% (25/330) were from collared peccary lymph nodes. Among the 11 isolates of R. equi from the white-lipped peccaries, 90.9% (n = 10/11) were obtained from the mesenteric lymph nodes, and only 9.1% (n = 1/10) were obtained from the mediastinal lymph nodes. In the 25 isolates of R. equi obtained from the collared peccaries, 40.0% (n = 10/25) were recovered from the mesenteric lymph nodes, 36% (n = 9/25) from the submandibular lymph nodes, and 24.0% (n = 6/25) from the mediastinal lymph nodes. No vapA, vapB, or vapN genes (plasmidless) or three host-associated types (pVAPA, pVAPB, and pVAPN) were identified among the R. equi isolates. Mycobacterium species were isolated in 3.03% (n = 10/330) of all the lymph nodes analyzed. Among the 10 mycobacterial isolates, 60% (n = 6/10) were from the white-lipped peccary lymph nodes, and 40% (n = 4/10) were from the collared peccary lymph nodes. Ten Mycobacterium species were detected by PCR-PRA with a predominance of M. avium type 1. Sequencing of the hsp65 and rpob genes revealed mycobacteria that were saprophytic (M. sinense and M. kumamotonense) and potentially pathogenic (M. colombiense and M. intracellulare) to humans and animals. To our knowledge, this is the first description of R. equi and/or mycobacterial species identified in the lymph nodes of peccary specimens. R. equi (plasmidless) and the mycobacterial species described here have been reported as causes of pulmonary and extrapulmonary infections in both immunocompetent and immunocompromised humans.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:18:35Z
2018-12-11T17:18:35Z
2018-08-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s11250-018-1562-2
Tropical Animal Health and Production, v. 50, n. 6, p. 1319-1326, 2018.
1573-7438
0049-4747
http://hdl.handle.net/11449/176018
10.1007/s11250-018-1562-2
2-s2.0-85044090451
2-s2.0-85044090451.pdf
url http://dx.doi.org/10.1007/s11250-018-1562-2
http://hdl.handle.net/11449/176018
identifier_str_mv Tropical Animal Health and Production, v. 50, n. 6, p. 1319-1326, 2018.
1573-7438
0049-4747
10.1007/s11250-018-1562-2
2-s2.0-85044090451
2-s2.0-85044090451.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Tropical Animal Health and Production
0,511
0,511
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1319-1326
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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