Determination of zinc in rice grains using DTZ staining and ImageJ software

Detalhes bibliográficos
Autor(a) principal: Duarte, Romulo F.
Data de Publicação: 2016
Outros Autores: Prom-u-Thai, Chanakan, Amaral, Douglas C., Faquin, Valdemar, Guilherme, Luiz R. G., Reis, Andre R. [UNESP], Alves, Eduardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jcs.2015.11.006
http://hdl.handle.net/11449/161430
Resumo: Dithizone (DTZ) staining is a rapid, simple, and inexpensive method that allows the histochemical localization of Zn. In this study, we evaluated genotypic variation in Zn concentration in brown rice grown in the field using atomic absorption spectrophotometer (AAS) as DTZ staining quantified using ImageJ software. We used dehusked grains from upland rice accessions widely cultivated in Brazil. The DTZ staining showed that the concentration of Zn varied within and between rice grains. The staining intensity index (Y) provided differences in localization of Zn across the grain regions. Zn concentration in brown rice varied in multiple regression analysis, showing major differences in index weighted by stained area of each region (YAW) for the embryo, endosperm, and aleurone, especially in the endosperm and aleurone regions due to a large portion of the area in the kernel. The total YAW among rice accessions was positively correlated with Zn concentration in grains by chemical analysis. The DTZ staining associated with imageJ software is a promising to estimate Zn concentration in different grain tissues. Thus,. this method may be useful for rapid screening of rice germplasms. (C) 2015 Elsevier Ltd. All rights reserved.
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spelling Determination of zinc in rice grains using DTZ staining and ImageJ softwareOryza sativa L.Zinc deficiencyStaining intensityBiofortificationDithizone (DTZ) staining is a rapid, simple, and inexpensive method that allows the histochemical localization of Zn. In this study, we evaluated genotypic variation in Zn concentration in brown rice grown in the field using atomic absorption spectrophotometer (AAS) as DTZ staining quantified using ImageJ software. We used dehusked grains from upland rice accessions widely cultivated in Brazil. The DTZ staining showed that the concentration of Zn varied within and between rice grains. The staining intensity index (Y) provided differences in localization of Zn across the grain regions. Zn concentration in brown rice varied in multiple regression analysis, showing major differences in index weighted by stained area of each region (YAW) for the embryo, endosperm, and aleurone, especially in the endosperm and aleurone regions due to a large portion of the area in the kernel. The total YAW among rice accessions was positively correlated with Zn concentration in grains by chemical analysis. The DTZ staining associated with imageJ software is a promising to estimate Zn concentration in different grain tissues. Thus,. this method may be useful for rapid screening of rice germplasms. (C) 2015 Elsevier Ltd. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)National Research University Programme of Thailand's Commission on Higher EducationChiang Mai Univ, Fac Agr, Dept Plant Sci & Nat Resources, Div Agron, Chiang Mai 50200, ThailandUniv Fed Lavras, Dept Soil Sci, POB 3037, BR-37200000 Lavras, MG, BrazilChiang Mai Univ, Lanna Rice Res Ctr, Chiang Mai 50200, ThailandSao Paulo State Univ, Fac Sci & Engn, Biosyst Engn, BR-17602496 Tupa, SP, BrazilUniv Fed Lavras, Dept Plant Pathol, POB 3037, BR-37200000 Lavras, MG, BrazilSao Paulo State Univ, Fac Sci & Engn, Biosyst Engn, BR-17602496 Tupa, SP, BrazilElsevier B.V.Chiang Mai UnivUniversidade Federal de Lavras (UFLA)Universidade Estadual Paulista (Unesp)Duarte, Romulo F.Prom-u-Thai, ChanakanAmaral, Douglas C.Faquin, ValdemarGuilherme, Luiz R. G.Reis, Andre R. [UNESP]Alves, Eduardo2018-11-26T16:32:43Z2018-11-26T16:32:43Z2016-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article53-58application/pdfhttp://dx.doi.org/10.1016/j.jcs.2015.11.006Journal Of Cereal Science. London: Academic Press Ltd- Elsevier Science Ltd, v. 68, p. 53-58, 2016.0733-5210http://hdl.handle.net/11449/16143010.1016/j.jcs.2015.11.006WOS:000374361800008WOS000374361800008.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Cereal Science1,121info:eu-repo/semantics/openAccess2024-06-10T14:49:30Zoai:repositorio.unesp.br:11449/161430Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:41:17.992875Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Determination of zinc in rice grains using DTZ staining and ImageJ software
title Determination of zinc in rice grains using DTZ staining and ImageJ software
spellingShingle Determination of zinc in rice grains using DTZ staining and ImageJ software
Duarte, Romulo F.
Oryza sativa L.
Zinc deficiency
Staining intensity
Biofortification
title_short Determination of zinc in rice grains using DTZ staining and ImageJ software
title_full Determination of zinc in rice grains using DTZ staining and ImageJ software
title_fullStr Determination of zinc in rice grains using DTZ staining and ImageJ software
title_full_unstemmed Determination of zinc in rice grains using DTZ staining and ImageJ software
title_sort Determination of zinc in rice grains using DTZ staining and ImageJ software
author Duarte, Romulo F.
author_facet Duarte, Romulo F.
Prom-u-Thai, Chanakan
Amaral, Douglas C.
Faquin, Valdemar
Guilherme, Luiz R. G.
Reis, Andre R. [UNESP]
Alves, Eduardo
author_role author
author2 Prom-u-Thai, Chanakan
Amaral, Douglas C.
Faquin, Valdemar
Guilherme, Luiz R. G.
Reis, Andre R. [UNESP]
Alves, Eduardo
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Chiang Mai Univ
Universidade Federal de Lavras (UFLA)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Duarte, Romulo F.
Prom-u-Thai, Chanakan
Amaral, Douglas C.
Faquin, Valdemar
Guilherme, Luiz R. G.
Reis, Andre R. [UNESP]
Alves, Eduardo
dc.subject.por.fl_str_mv Oryza sativa L.
Zinc deficiency
Staining intensity
Biofortification
topic Oryza sativa L.
Zinc deficiency
Staining intensity
Biofortification
description Dithizone (DTZ) staining is a rapid, simple, and inexpensive method that allows the histochemical localization of Zn. In this study, we evaluated genotypic variation in Zn concentration in brown rice grown in the field using atomic absorption spectrophotometer (AAS) as DTZ staining quantified using ImageJ software. We used dehusked grains from upland rice accessions widely cultivated in Brazil. The DTZ staining showed that the concentration of Zn varied within and between rice grains. The staining intensity index (Y) provided differences in localization of Zn across the grain regions. Zn concentration in brown rice varied in multiple regression analysis, showing major differences in index weighted by stained area of each region (YAW) for the embryo, endosperm, and aleurone, especially in the endosperm and aleurone regions due to a large portion of the area in the kernel. The total YAW among rice accessions was positively correlated with Zn concentration in grains by chemical analysis. The DTZ staining associated with imageJ software is a promising to estimate Zn concentration in different grain tissues. Thus,. this method may be useful for rapid screening of rice germplasms. (C) 2015 Elsevier Ltd. All rights reserved.
publishDate 2016
dc.date.none.fl_str_mv 2016-03-01
2018-11-26T16:32:43Z
2018-11-26T16:32:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jcs.2015.11.006
Journal Of Cereal Science. London: Academic Press Ltd- Elsevier Science Ltd, v. 68, p. 53-58, 2016.
0733-5210
http://hdl.handle.net/11449/161430
10.1016/j.jcs.2015.11.006
WOS:000374361800008
WOS000374361800008.pdf
url http://dx.doi.org/10.1016/j.jcs.2015.11.006
http://hdl.handle.net/11449/161430
identifier_str_mv Journal Of Cereal Science. London: Academic Press Ltd- Elsevier Science Ltd, v. 68, p. 53-58, 2016.
0733-5210
10.1016/j.jcs.2015.11.006
WOS:000374361800008
WOS000374361800008.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Cereal Science
1,121
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 53-58
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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