MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma

Detalhes bibliográficos
Autor(a) principal: Donís, Sergio Piñeiro
Data de Publicação: 2021
Outros Autores: González, Alba Pérez, Alves, Monica Ghislaine Oliveira, Do Carmo Carvalho, Bruna F., Ferreira, Camila C.P., Almeida, Janete Dias [UNESP], Iruegas, Elena Padín, Petronacci, Cintia M. Chamorro, Peñaranda, José M. Suárez, Sayáns, Mario Pérez
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1097/PAI.0000000000000929
http://hdl.handle.net/11449/233567
Resumo: Background: DNA damage is accumulated in the cells over time as the result of both exogenous and endogenous factors. The objective of this study was to analyze the immunohistochemical expression of the repair proteins in oral leukoplakia (OL) and oral squamous cell carcinoma (OSCC). Materials and Methods: Paraffin blocks were selected from the archives of the Laboratory of Hospital Clinico Universitario de Santiago de Compostela, Spain. The sample was composed of 16 cases of OL without dysplasia, 14 cases of OL with dysplasia, and 15 cases of OSCC. The patients' clinical data were collected and immunohistochemical analysis was performed for MLH1, MSH2, MRE11, and XRCC1. The data were submitted to the χ2and the Kruskal-Wallis (P≤0.05) tests. Results: MSH2 was overexpressed in OSCC (P=0.020) and was positive in 100% of patients with OL with dysplasia or OSCC (P=0.019). Positivity for MLH1 was significantly associated with comorbidity (P=0.040), especially in patients who presented with 2 or more pathologies (P=0.028). XRCC1 positivity was also associated with comorbidity (P=0.039). No significant associations were found for the MRE11A expression. Although the simultaneous positivity for the 4 markers was observed in presence of comorbidities (P=0.006). Conclusions: This study supports the effect of the overexpression of MSH2 protein in samples of OL with dysplasia and OSCC, most notably in patients who present with comorbidities and negativity for OL without dysplasia.
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spelling MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinomacancer and precancerDNA repairimmunopathologymucosal diseasesoral squamous cell carcinomaBackground: DNA damage is accumulated in the cells over time as the result of both exogenous and endogenous factors. The objective of this study was to analyze the immunohistochemical expression of the repair proteins in oral leukoplakia (OL) and oral squamous cell carcinoma (OSCC). Materials and Methods: Paraffin blocks were selected from the archives of the Laboratory of Hospital Clinico Universitario de Santiago de Compostela, Spain. The sample was composed of 16 cases of OL without dysplasia, 14 cases of OL with dysplasia, and 15 cases of OSCC. The patients' clinical data were collected and immunohistochemical analysis was performed for MLH1, MSH2, MRE11, and XRCC1. The data were submitted to the χ2and the Kruskal-Wallis (P≤0.05) tests. Results: MSH2 was overexpressed in OSCC (P=0.020) and was positive in 100% of patients with OL with dysplasia or OSCC (P=0.019). Positivity for MLH1 was significantly associated with comorbidity (P=0.040), especially in patients who presented with 2 or more pathologies (P=0.028). XRCC1 positivity was also associated with comorbidity (P=0.039). No significant associations were found for the MRE11A expression. Although the simultaneous positivity for the 4 markers was observed in presence of comorbidities (P=0.006). Conclusions: This study supports the effect of the overexpression of MSH2 protein in samples of OL with dysplasia and OSCC, most notably in patients who present with comorbidities and negativity for OL without dysplasia.Oral Medicine UnitTechnology Research Center (NPT) Mogi das Cruzes University Mogi das Cruzes, 200 Dr. C ndido Xavier de Almeida Souza AvenueDepartment of Pathology Clinical Hospital Santiago de CompostelaDepartment of Forensic Sciences and Pathology University of Santiago de CompostelaTranslational Oncology Laboratory Idichus Foundation Santiago de CompostelaSchool of Medicine Anhembi Morumbi UniversityDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP)Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP)Oral Medicine UnitMogi das CruzesSantiago de CompostelaUniversity of Santiago de CompostelaAnhembi Morumbi UniversityUniversidade Estadual Paulista (UNESP)Donís, Sergio PiñeiroGonzález, Alba PérezAlves, Monica Ghislaine OliveiraDo Carmo Carvalho, Bruna F.Ferreira, Camila C.P.Almeida, Janete Dias [UNESP]Iruegas, Elena PadínPetronacci, Cintia M. ChamorroPeñaranda, José M. SuárezSayáns, Mario Pérez2022-05-01T09:30:47Z2022-05-01T09:30:47Z2021-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article613-618http://dx.doi.org/10.1097/PAI.0000000000000929Applied Immunohistochemistry and Molecular Morphology, v. 29, n. 8, p. 613-618, 2021.1533-40581541-2016http://hdl.handle.net/11449/23356710.1097/PAI.00000000000009292-s2.0-85115435609Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengApplied Immunohistochemistry and Molecular Morphologyinfo:eu-repo/semantics/openAccess2022-05-01T09:30:47Zoai:repositorio.unesp.br:11449/233567Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-05-01T09:30:47Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma
title MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma
spellingShingle MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma
Donís, Sergio Piñeiro
cancer and precancer
DNA repair
immunopathology
mucosal diseases
oral squamous cell carcinoma
title_short MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma
title_full MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma
title_fullStr MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma
title_full_unstemmed MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma
title_sort MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma
author Donís, Sergio Piñeiro
author_facet Donís, Sergio Piñeiro
González, Alba Pérez
Alves, Monica Ghislaine Oliveira
Do Carmo Carvalho, Bruna F.
Ferreira, Camila C.P.
Almeida, Janete Dias [UNESP]
Iruegas, Elena Padín
Petronacci, Cintia M. Chamorro
Peñaranda, José M. Suárez
Sayáns, Mario Pérez
author_role author
author2 González, Alba Pérez
Alves, Monica Ghislaine Oliveira
Do Carmo Carvalho, Bruna F.
Ferreira, Camila C.P.
Almeida, Janete Dias [UNESP]
Iruegas, Elena Padín
Petronacci, Cintia M. Chamorro
Peñaranda, José M. Suárez
Sayáns, Mario Pérez
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Oral Medicine Unit
Mogi das Cruzes
Santiago de Compostela
University of Santiago de Compostela
Anhembi Morumbi University
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Donís, Sergio Piñeiro
González, Alba Pérez
Alves, Monica Ghislaine Oliveira
Do Carmo Carvalho, Bruna F.
Ferreira, Camila C.P.
Almeida, Janete Dias [UNESP]
Iruegas, Elena Padín
Petronacci, Cintia M. Chamorro
Peñaranda, José M. Suárez
Sayáns, Mario Pérez
dc.subject.por.fl_str_mv cancer and precancer
DNA repair
immunopathology
mucosal diseases
oral squamous cell carcinoma
topic cancer and precancer
DNA repair
immunopathology
mucosal diseases
oral squamous cell carcinoma
description Background: DNA damage is accumulated in the cells over time as the result of both exogenous and endogenous factors. The objective of this study was to analyze the immunohistochemical expression of the repair proteins in oral leukoplakia (OL) and oral squamous cell carcinoma (OSCC). Materials and Methods: Paraffin blocks were selected from the archives of the Laboratory of Hospital Clinico Universitario de Santiago de Compostela, Spain. The sample was composed of 16 cases of OL without dysplasia, 14 cases of OL with dysplasia, and 15 cases of OSCC. The patients' clinical data were collected and immunohistochemical analysis was performed for MLH1, MSH2, MRE11, and XRCC1. The data were submitted to the χ2and the Kruskal-Wallis (P≤0.05) tests. Results: MSH2 was overexpressed in OSCC (P=0.020) and was positive in 100% of patients with OL with dysplasia or OSCC (P=0.019). Positivity for MLH1 was significantly associated with comorbidity (P=0.040), especially in patients who presented with 2 or more pathologies (P=0.028). XRCC1 positivity was also associated with comorbidity (P=0.039). No significant associations were found for the MRE11A expression. Although the simultaneous positivity for the 4 markers was observed in presence of comorbidities (P=0.006). Conclusions: This study supports the effect of the overexpression of MSH2 protein in samples of OL with dysplasia and OSCC, most notably in patients who present with comorbidities and negativity for OL without dysplasia.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-01
2022-05-01T09:30:47Z
2022-05-01T09:30:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1097/PAI.0000000000000929
Applied Immunohistochemistry and Molecular Morphology, v. 29, n. 8, p. 613-618, 2021.
1533-4058
1541-2016
http://hdl.handle.net/11449/233567
10.1097/PAI.0000000000000929
2-s2.0-85115435609
url http://dx.doi.org/10.1097/PAI.0000000000000929
http://hdl.handle.net/11449/233567
identifier_str_mv Applied Immunohistochemistry and Molecular Morphology, v. 29, n. 8, p. 613-618, 2021.
1533-4058
1541-2016
10.1097/PAI.0000000000000929
2-s2.0-85115435609
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Applied Immunohistochemistry and Molecular Morphology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 613-618
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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