MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1097/PAI.0000000000000929 http://hdl.handle.net/11449/233567 |
Resumo: | Background: DNA damage is accumulated in the cells over time as the result of both exogenous and endogenous factors. The objective of this study was to analyze the immunohistochemical expression of the repair proteins in oral leukoplakia (OL) and oral squamous cell carcinoma (OSCC). Materials and Methods: Paraffin blocks were selected from the archives of the Laboratory of Hospital Clinico Universitario de Santiago de Compostela, Spain. The sample was composed of 16 cases of OL without dysplasia, 14 cases of OL with dysplasia, and 15 cases of OSCC. The patients' clinical data were collected and immunohistochemical analysis was performed for MLH1, MSH2, MRE11, and XRCC1. The data were submitted to the χ2and the Kruskal-Wallis (P≤0.05) tests. Results: MSH2 was overexpressed in OSCC (P=0.020) and was positive in 100% of patients with OL with dysplasia or OSCC (P=0.019). Positivity for MLH1 was significantly associated with comorbidity (P=0.040), especially in patients who presented with 2 or more pathologies (P=0.028). XRCC1 positivity was also associated with comorbidity (P=0.039). No significant associations were found for the MRE11A expression. Although the simultaneous positivity for the 4 markers was observed in presence of comorbidities (P=0.006). Conclusions: This study supports the effect of the overexpression of MSH2 protein in samples of OL with dysplasia and OSCC, most notably in patients who present with comorbidities and negativity for OL without dysplasia. |
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MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinomacancer and precancerDNA repairimmunopathologymucosal diseasesoral squamous cell carcinomaBackground: DNA damage is accumulated in the cells over time as the result of both exogenous and endogenous factors. The objective of this study was to analyze the immunohistochemical expression of the repair proteins in oral leukoplakia (OL) and oral squamous cell carcinoma (OSCC). Materials and Methods: Paraffin blocks were selected from the archives of the Laboratory of Hospital Clinico Universitario de Santiago de Compostela, Spain. The sample was composed of 16 cases of OL without dysplasia, 14 cases of OL with dysplasia, and 15 cases of OSCC. The patients' clinical data were collected and immunohistochemical analysis was performed for MLH1, MSH2, MRE11, and XRCC1. The data were submitted to the χ2and the Kruskal-Wallis (P≤0.05) tests. Results: MSH2 was overexpressed in OSCC (P=0.020) and was positive in 100% of patients with OL with dysplasia or OSCC (P=0.019). Positivity for MLH1 was significantly associated with comorbidity (P=0.040), especially in patients who presented with 2 or more pathologies (P=0.028). XRCC1 positivity was also associated with comorbidity (P=0.039). No significant associations were found for the MRE11A expression. Although the simultaneous positivity for the 4 markers was observed in presence of comorbidities (P=0.006). Conclusions: This study supports the effect of the overexpression of MSH2 protein in samples of OL with dysplasia and OSCC, most notably in patients who present with comorbidities and negativity for OL without dysplasia.Oral Medicine UnitTechnology Research Center (NPT) Mogi das Cruzes University Mogi das Cruzes, 200 Dr. C ndido Xavier de Almeida Souza AvenueDepartment of Pathology Clinical Hospital Santiago de CompostelaDepartment of Forensic Sciences and Pathology University of Santiago de CompostelaTranslational Oncology Laboratory Idichus Foundation Santiago de CompostelaSchool of Medicine Anhembi Morumbi UniversityDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP)Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP)Oral Medicine UnitMogi das CruzesSantiago de CompostelaUniversity of Santiago de CompostelaAnhembi Morumbi UniversityUniversidade Estadual Paulista (UNESP)Donís, Sergio PiñeiroGonzález, Alba PérezAlves, Monica Ghislaine OliveiraDo Carmo Carvalho, Bruna F.Ferreira, Camila C.P.Almeida, Janete Dias [UNESP]Iruegas, Elena PadínPetronacci, Cintia M. ChamorroPeñaranda, José M. SuárezSayáns, Mario Pérez2022-05-01T09:30:47Z2022-05-01T09:30:47Z2021-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article613-618http://dx.doi.org/10.1097/PAI.0000000000000929Applied Immunohistochemistry and Molecular Morphology, v. 29, n. 8, p. 613-618, 2021.1533-40581541-2016http://hdl.handle.net/11449/23356710.1097/PAI.00000000000009292-s2.0-85115435609Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengApplied Immunohistochemistry and Molecular Morphologyinfo:eu-repo/semantics/openAccess2022-05-01T09:30:47Zoai:repositorio.unesp.br:11449/233567Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-05-01T09:30:47Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma |
title |
MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma |
spellingShingle |
MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma Donís, Sergio Piñeiro cancer and precancer DNA repair immunopathology mucosal diseases oral squamous cell carcinoma |
title_short |
MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma |
title_full |
MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma |
title_fullStr |
MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma |
title_full_unstemmed |
MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma |
title_sort |
MLH1, MSH2, MRE11, and XRCC1 in Oral Leukoplakia and Oral Squamous Cell Carcinoma |
author |
Donís, Sergio Piñeiro |
author_facet |
Donís, Sergio Piñeiro González, Alba Pérez Alves, Monica Ghislaine Oliveira Do Carmo Carvalho, Bruna F. Ferreira, Camila C.P. Almeida, Janete Dias [UNESP] Iruegas, Elena Padín Petronacci, Cintia M. Chamorro Peñaranda, José M. Suárez Sayáns, Mario Pérez |
author_role |
author |
author2 |
González, Alba Pérez Alves, Monica Ghislaine Oliveira Do Carmo Carvalho, Bruna F. Ferreira, Camila C.P. Almeida, Janete Dias [UNESP] Iruegas, Elena Padín Petronacci, Cintia M. Chamorro Peñaranda, José M. Suárez Sayáns, Mario Pérez |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Oral Medicine Unit Mogi das Cruzes Santiago de Compostela University of Santiago de Compostela Anhembi Morumbi University Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Donís, Sergio Piñeiro González, Alba Pérez Alves, Monica Ghislaine Oliveira Do Carmo Carvalho, Bruna F. Ferreira, Camila C.P. Almeida, Janete Dias [UNESP] Iruegas, Elena Padín Petronacci, Cintia M. Chamorro Peñaranda, José M. Suárez Sayáns, Mario Pérez |
dc.subject.por.fl_str_mv |
cancer and precancer DNA repair immunopathology mucosal diseases oral squamous cell carcinoma |
topic |
cancer and precancer DNA repair immunopathology mucosal diseases oral squamous cell carcinoma |
description |
Background: DNA damage is accumulated in the cells over time as the result of both exogenous and endogenous factors. The objective of this study was to analyze the immunohistochemical expression of the repair proteins in oral leukoplakia (OL) and oral squamous cell carcinoma (OSCC). Materials and Methods: Paraffin blocks were selected from the archives of the Laboratory of Hospital Clinico Universitario de Santiago de Compostela, Spain. The sample was composed of 16 cases of OL without dysplasia, 14 cases of OL with dysplasia, and 15 cases of OSCC. The patients' clinical data were collected and immunohistochemical analysis was performed for MLH1, MSH2, MRE11, and XRCC1. The data were submitted to the χ2and the Kruskal-Wallis (P≤0.05) tests. Results: MSH2 was overexpressed in OSCC (P=0.020) and was positive in 100% of patients with OL with dysplasia or OSCC (P=0.019). Positivity for MLH1 was significantly associated with comorbidity (P=0.040), especially in patients who presented with 2 or more pathologies (P=0.028). XRCC1 positivity was also associated with comorbidity (P=0.039). No significant associations were found for the MRE11A expression. Although the simultaneous positivity for the 4 markers was observed in presence of comorbidities (P=0.006). Conclusions: This study supports the effect of the overexpression of MSH2 protein in samples of OL with dysplasia and OSCC, most notably in patients who present with comorbidities and negativity for OL without dysplasia. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-01 2022-05-01T09:30:47Z 2022-05-01T09:30:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1097/PAI.0000000000000929 Applied Immunohistochemistry and Molecular Morphology, v. 29, n. 8, p. 613-618, 2021. 1533-4058 1541-2016 http://hdl.handle.net/11449/233567 10.1097/PAI.0000000000000929 2-s2.0-85115435609 |
url |
http://dx.doi.org/10.1097/PAI.0000000000000929 http://hdl.handle.net/11449/233567 |
identifier_str_mv |
Applied Immunohistochemistry and Molecular Morphology, v. 29, n. 8, p. 613-618, 2021. 1533-4058 1541-2016 10.1097/PAI.0000000000000929 2-s2.0-85115435609 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Applied Immunohistochemistry and Molecular Morphology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
613-618 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965617539776512 |