Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1807-3107bor-2021.vol35.0062 http://hdl.handle.net/11449/211923 |
Resumo: | This in vitro study evaluated the impact of TiO2 nanotubes (n-TiO2) incorporated into glass ionomer cement (GIC) on Streptococcus mutans (S. mutans) characteristics at cellular and molecular levels. n-TiO2, synthesized by the alkaline method (20 nm in size), was added to Ketac Molar EasyMix® at 0%, 3%, 5%, and 7% by weight. S. mutans strains were cultured on GIC disks with addition or not of n-TiO2 for 1, 3, and 7 days and the following parameters were assessed: inhibition halo (mm) (n=3/group); cell viability (live/dead) (n=5/group); cell morphology (SEM) (n=3/group); and gene expression by real-time PCR (vicR, covR, gtfB, gtfC, and gtfD) (n=6/group). The data were analyzed by the Kruskal-Wallis test, repeated-measures ANOVA or two-way ANOVA, and Tukey’s and Dunn’s post-hoc tests (α=0.05). The agar diffusion test showed a higher antibacterial property for 5% n-TiO2 compared with 3% and 7% (p<0.05) with no effect of time (1, 3, and 7 days). The cell number was significantly affected by all n-TiO2 groups, while viability was mostly affected by 3% and 5% n-TiO2, which also affected cell morphology and organization. Real-time PCR demonstrated that n-TiO2 reduced the expression of covR when compared with GIC with no n-TiO2 (p<0.05), with no effect of time, except for 3% n-TiO2 on vicR expression. Within-group and between-group analyses revealed n-TiO2 did not affect mRNA levels of gtfB, gtfC, and gtfD (p>0.05). Incorporation of n-TiO2 at 3% and 5% potentially affected S. mutans viability and the expression of key genes for bacterial survival and growth, improving the anticariogenic properties of GIC. |
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Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulenceNanotechnologyGlass Ionomer CementsTitaniumGene ExpressionThis in vitro study evaluated the impact of TiO2 nanotubes (n-TiO2) incorporated into glass ionomer cement (GIC) on Streptococcus mutans (S. mutans) characteristics at cellular and molecular levels. n-TiO2, synthesized by the alkaline method (20 nm in size), was added to Ketac Molar EasyMix® at 0%, 3%, 5%, and 7% by weight. S. mutans strains were cultured on GIC disks with addition or not of n-TiO2 for 1, 3, and 7 days and the following parameters were assessed: inhibition halo (mm) (n=3/group); cell viability (live/dead) (n=5/group); cell morphology (SEM) (n=3/group); and gene expression by real-time PCR (vicR, covR, gtfB, gtfC, and gtfD) (n=6/group). The data were analyzed by the Kruskal-Wallis test, repeated-measures ANOVA or two-way ANOVA, and Tukey’s and Dunn’s post-hoc tests (α=0.05). The agar diffusion test showed a higher antibacterial property for 5% n-TiO2 compared with 3% and 7% (p<0.05) with no effect of time (1, 3, and 7 days). The cell number was significantly affected by all n-TiO2 groups, while viability was mostly affected by 3% and 5% n-TiO2, which also affected cell morphology and organization. Real-time PCR demonstrated that n-TiO2 reduced the expression of covR when compared with GIC with no n-TiO2 (p<0.05), with no effect of time, except for 3% n-TiO2 on vicR expression. Within-group and between-group analyses revealed n-TiO2 did not affect mRNA levels of gtfB, gtfC, and gtfD (p>0.05). Incorporation of n-TiO2 at 3% and 5% potentially affected S. mutans viability and the expression of key genes for bacterial survival and growth, improving the anticariogenic properties of GIC.São Paulo Research Support FoundationConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual de Campinas, Piracicaba Dental SchoolFaculdade São Leopoldo Mandic, Dental Research CenterUniversidade Estadual Paulista Júlio Mesquita Filho, School of SciencesUniversidade Paulista, School of DentistryUniversidade Estadual Paulista Júlio Mesquita Filho, School of SciencesSão Paulo Research Support Foundation: 16/13786-0São Paulo Research Support Foundation: 19/14078-8CNPq: 2018-2019Sociedade Brasileira de Pesquisa Odontológica - SBPqOUniversidade Estadual de Campinas (UNICAMP)Faculdade São Leopoldo MandicUniversidade Estadual Paulista (Unesp)Universidade PaulistaAraújo, Isaac Jordão De SouzaRicardo, Mariana GallanteGomes, Orisson Ponce [UNESP]Giovani, Priscila AlvesPuppin-rontani, JúliaPecorari, Vanessa AriasMartinez, Elizabeth FerreiraNapimoga, Marcelo HenriqueNociti, Francisco HumbertoPuppin-rontani, Regina MariaLisboa-filho, Paulo Noronha [UNESP]Kantovitz, Kamila Rosamilia2021-07-14T10:31:44Z2021-07-14T10:31:44Z2021-06-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/1807-3107bor-2021.vol35.0062Brazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 35, p. -, 2021.1806-83241807-3107http://hdl.handle.net/11449/21192310.1590/1807-3107bor-2021.vol35.0062S1806-83242021000100253S1806-83242021000100253.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Oral Researchinfo:eu-repo/semantics/openAccess2023-12-22T06:22:27Zoai:repositorio.unesp.br:11449/211923Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:00:34.837530Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence |
title |
Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence |
spellingShingle |
Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence Araújo, Isaac Jordão De Souza Nanotechnology Glass Ionomer Cements Titanium Gene Expression |
title_short |
Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence |
title_full |
Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence |
title_fullStr |
Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence |
title_full_unstemmed |
Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence |
title_sort |
Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence |
author |
Araújo, Isaac Jordão De Souza |
author_facet |
Araújo, Isaac Jordão De Souza Ricardo, Mariana Gallante Gomes, Orisson Ponce [UNESP] Giovani, Priscila Alves Puppin-rontani, Júlia Pecorari, Vanessa Arias Martinez, Elizabeth Ferreira Napimoga, Marcelo Henrique Nociti, Francisco Humberto Puppin-rontani, Regina Maria Lisboa-filho, Paulo Noronha [UNESP] Kantovitz, Kamila Rosamilia |
author_role |
author |
author2 |
Ricardo, Mariana Gallante Gomes, Orisson Ponce [UNESP] Giovani, Priscila Alves Puppin-rontani, Júlia Pecorari, Vanessa Arias Martinez, Elizabeth Ferreira Napimoga, Marcelo Henrique Nociti, Francisco Humberto Puppin-rontani, Regina Maria Lisboa-filho, Paulo Noronha [UNESP] Kantovitz, Kamila Rosamilia |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Faculdade São Leopoldo Mandic Universidade Estadual Paulista (Unesp) Universidade Paulista |
dc.contributor.author.fl_str_mv |
Araújo, Isaac Jordão De Souza Ricardo, Mariana Gallante Gomes, Orisson Ponce [UNESP] Giovani, Priscila Alves Puppin-rontani, Júlia Pecorari, Vanessa Arias Martinez, Elizabeth Ferreira Napimoga, Marcelo Henrique Nociti, Francisco Humberto Puppin-rontani, Regina Maria Lisboa-filho, Paulo Noronha [UNESP] Kantovitz, Kamila Rosamilia |
dc.subject.por.fl_str_mv |
Nanotechnology Glass Ionomer Cements Titanium Gene Expression |
topic |
Nanotechnology Glass Ionomer Cements Titanium Gene Expression |
description |
This in vitro study evaluated the impact of TiO2 nanotubes (n-TiO2) incorporated into glass ionomer cement (GIC) on Streptococcus mutans (S. mutans) characteristics at cellular and molecular levels. n-TiO2, synthesized by the alkaline method (20 nm in size), was added to Ketac Molar EasyMix® at 0%, 3%, 5%, and 7% by weight. S. mutans strains were cultured on GIC disks with addition or not of n-TiO2 for 1, 3, and 7 days and the following parameters were assessed: inhibition halo (mm) (n=3/group); cell viability (live/dead) (n=5/group); cell morphology (SEM) (n=3/group); and gene expression by real-time PCR (vicR, covR, gtfB, gtfC, and gtfD) (n=6/group). The data were analyzed by the Kruskal-Wallis test, repeated-measures ANOVA or two-way ANOVA, and Tukey’s and Dunn’s post-hoc tests (α=0.05). The agar diffusion test showed a higher antibacterial property for 5% n-TiO2 compared with 3% and 7% (p<0.05) with no effect of time (1, 3, and 7 days). The cell number was significantly affected by all n-TiO2 groups, while viability was mostly affected by 3% and 5% n-TiO2, which also affected cell morphology and organization. Real-time PCR demonstrated that n-TiO2 reduced the expression of covR when compared with GIC with no n-TiO2 (p<0.05), with no effect of time, except for 3% n-TiO2 on vicR expression. Within-group and between-group analyses revealed n-TiO2 did not affect mRNA levels of gtfB, gtfC, and gtfD (p>0.05). Incorporation of n-TiO2 at 3% and 5% potentially affected S. mutans viability and the expression of key genes for bacterial survival and growth, improving the anticariogenic properties of GIC. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-14T10:31:44Z 2021-07-14T10:31:44Z 2021-06-14 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1807-3107bor-2021.vol35.0062 Brazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 35, p. -, 2021. 1806-8324 1807-3107 http://hdl.handle.net/11449/211923 10.1590/1807-3107bor-2021.vol35.0062 S1806-83242021000100253 S1806-83242021000100253.pdf |
url |
http://dx.doi.org/10.1590/1807-3107bor-2021.vol35.0062 http://hdl.handle.net/11449/211923 |
identifier_str_mv |
Brazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 35, p. -, 2021. 1806-8324 1807-3107 10.1590/1807-3107bor-2021.vol35.0062 S1806-83242021000100253 S1806-83242021000100253.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Oral Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
- application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129273542737920 |