Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence

Detalhes bibliográficos
Autor(a) principal: Araújo, Isaac Jordão De Souza
Data de Publicação: 2021
Outros Autores: Ricardo, Mariana Gallante, Gomes, Orisson Ponce [UNESP], Giovani, Priscila Alves, Puppin-rontani, Júlia, Pecorari, Vanessa Arias, Martinez, Elizabeth Ferreira, Napimoga, Marcelo Henrique, Nociti, Francisco Humberto, Puppin-rontani, Regina Maria, Lisboa-filho, Paulo Noronha [UNESP], Kantovitz, Kamila Rosamilia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1807-3107bor-2021.vol35.0062
http://hdl.handle.net/11449/211923
Resumo: This in vitro study evaluated the impact of TiO2 nanotubes (n-TiO2) incorporated into glass ionomer cement (GIC) on Streptococcus mutans (S. mutans) characteristics at cellular and molecular levels. n-TiO2, synthesized by the alkaline method (20 nm in size), was added to Ketac Molar EasyMix® at 0%, 3%, 5%, and 7% by weight. S. mutans strains were cultured on GIC disks with addition or not of n-TiO2 for 1, 3, and 7 days and the following parameters were assessed: inhibition halo (mm) (n=3/group); cell viability (live/dead) (n=5/group); cell morphology (SEM) (n=3/group); and gene expression by real-time PCR (vicR, covR, gtfB, gtfC, and gtfD) (n=6/group). The data were analyzed by the Kruskal-Wallis test, repeated-measures ANOVA or two-way ANOVA, and Tukey’s and Dunn’s post-hoc tests (α=0.05). The agar diffusion test showed a higher antibacterial property for 5% n-TiO2 compared with 3% and 7% (p<0.05) with no effect of time (1, 3, and 7 days). The cell number was significantly affected by all n-TiO2 groups, while viability was mostly affected by 3% and 5% n-TiO2, which also affected cell morphology and organization. Real-time PCR demonstrated that n-TiO2 reduced the expression of covR when compared with GIC with no n-TiO2 (p<0.05), with no effect of time, except for 3% n-TiO2 on vicR expression. Within-group and between-group analyses revealed n-TiO2 did not affect mRNA levels of gtfB, gtfC, and gtfD (p>0.05). Incorporation of n-TiO2 at 3% and 5% potentially affected S. mutans viability and the expression of key genes for bacterial survival and growth, improving the anticariogenic properties of GIC.
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spelling Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulenceNanotechnologyGlass Ionomer CementsTitaniumGene ExpressionThis in vitro study evaluated the impact of TiO2 nanotubes (n-TiO2) incorporated into glass ionomer cement (GIC) on Streptococcus mutans (S. mutans) characteristics at cellular and molecular levels. n-TiO2, synthesized by the alkaline method (20 nm in size), was added to Ketac Molar EasyMix® at 0%, 3%, 5%, and 7% by weight. S. mutans strains were cultured on GIC disks with addition or not of n-TiO2 for 1, 3, and 7 days and the following parameters were assessed: inhibition halo (mm) (n=3/group); cell viability (live/dead) (n=5/group); cell morphology (SEM) (n=3/group); and gene expression by real-time PCR (vicR, covR, gtfB, gtfC, and gtfD) (n=6/group). The data were analyzed by the Kruskal-Wallis test, repeated-measures ANOVA or two-way ANOVA, and Tukey’s and Dunn’s post-hoc tests (α=0.05). The agar diffusion test showed a higher antibacterial property for 5% n-TiO2 compared with 3% and 7% (p<0.05) with no effect of time (1, 3, and 7 days). The cell number was significantly affected by all n-TiO2 groups, while viability was mostly affected by 3% and 5% n-TiO2, which also affected cell morphology and organization. Real-time PCR demonstrated that n-TiO2 reduced the expression of covR when compared with GIC with no n-TiO2 (p<0.05), with no effect of time, except for 3% n-TiO2 on vicR expression. Within-group and between-group analyses revealed n-TiO2 did not affect mRNA levels of gtfB, gtfC, and gtfD (p>0.05). Incorporation of n-TiO2 at 3% and 5% potentially affected S. mutans viability and the expression of key genes for bacterial survival and growth, improving the anticariogenic properties of GIC.São Paulo Research Support FoundationConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual de Campinas, Piracicaba Dental SchoolFaculdade São Leopoldo Mandic, Dental Research CenterUniversidade Estadual Paulista Júlio Mesquita Filho, School of SciencesUniversidade Paulista, School of DentistryUniversidade Estadual Paulista Júlio Mesquita Filho, School of SciencesSão Paulo Research Support Foundation: 16/13786-0São Paulo Research Support Foundation: 19/14078-8CNPq: 2018-2019Sociedade Brasileira de Pesquisa Odontológica - SBPqOUniversidade Estadual de Campinas (UNICAMP)Faculdade São Leopoldo MandicUniversidade Estadual Paulista (Unesp)Universidade PaulistaAraújo, Isaac Jordão De SouzaRicardo, Mariana GallanteGomes, Orisson Ponce [UNESP]Giovani, Priscila AlvesPuppin-rontani, JúliaPecorari, Vanessa AriasMartinez, Elizabeth FerreiraNapimoga, Marcelo HenriqueNociti, Francisco HumbertoPuppin-rontani, Regina MariaLisboa-filho, Paulo Noronha [UNESP]Kantovitz, Kamila Rosamilia2021-07-14T10:31:44Z2021-07-14T10:31:44Z2021-06-14info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/1807-3107bor-2021.vol35.0062Brazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 35, p. -, 2021.1806-83241807-3107http://hdl.handle.net/11449/21192310.1590/1807-3107bor-2021.vol35.0062S1806-83242021000100253S1806-83242021000100253.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Oral Researchinfo:eu-repo/semantics/openAccess2023-12-22T06:22:27Zoai:repositorio.unesp.br:11449/211923Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:00:34.837530Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence
title Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence
spellingShingle Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence
Araújo, Isaac Jordão De Souza
Nanotechnology
Glass Ionomer Cements
Titanium
Gene Expression
title_short Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence
title_full Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence
title_fullStr Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence
title_full_unstemmed Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence
title_sort Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence
author Araújo, Isaac Jordão De Souza
author_facet Araújo, Isaac Jordão De Souza
Ricardo, Mariana Gallante
Gomes, Orisson Ponce [UNESP]
Giovani, Priscila Alves
Puppin-rontani, Júlia
Pecorari, Vanessa Arias
Martinez, Elizabeth Ferreira
Napimoga, Marcelo Henrique
Nociti, Francisco Humberto
Puppin-rontani, Regina Maria
Lisboa-filho, Paulo Noronha [UNESP]
Kantovitz, Kamila Rosamilia
author_role author
author2 Ricardo, Mariana Gallante
Gomes, Orisson Ponce [UNESP]
Giovani, Priscila Alves
Puppin-rontani, Júlia
Pecorari, Vanessa Arias
Martinez, Elizabeth Ferreira
Napimoga, Marcelo Henrique
Nociti, Francisco Humberto
Puppin-rontani, Regina Maria
Lisboa-filho, Paulo Noronha [UNESP]
Kantovitz, Kamila Rosamilia
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Faculdade São Leopoldo Mandic
Universidade Estadual Paulista (Unesp)
Universidade Paulista
dc.contributor.author.fl_str_mv Araújo, Isaac Jordão De Souza
Ricardo, Mariana Gallante
Gomes, Orisson Ponce [UNESP]
Giovani, Priscila Alves
Puppin-rontani, Júlia
Pecorari, Vanessa Arias
Martinez, Elizabeth Ferreira
Napimoga, Marcelo Henrique
Nociti, Francisco Humberto
Puppin-rontani, Regina Maria
Lisboa-filho, Paulo Noronha [UNESP]
Kantovitz, Kamila Rosamilia
dc.subject.por.fl_str_mv Nanotechnology
Glass Ionomer Cements
Titanium
Gene Expression
topic Nanotechnology
Glass Ionomer Cements
Titanium
Gene Expression
description This in vitro study evaluated the impact of TiO2 nanotubes (n-TiO2) incorporated into glass ionomer cement (GIC) on Streptococcus mutans (S. mutans) characteristics at cellular and molecular levels. n-TiO2, synthesized by the alkaline method (20 nm in size), was added to Ketac Molar EasyMix® at 0%, 3%, 5%, and 7% by weight. S. mutans strains were cultured on GIC disks with addition or not of n-TiO2 for 1, 3, and 7 days and the following parameters were assessed: inhibition halo (mm) (n=3/group); cell viability (live/dead) (n=5/group); cell morphology (SEM) (n=3/group); and gene expression by real-time PCR (vicR, covR, gtfB, gtfC, and gtfD) (n=6/group). The data were analyzed by the Kruskal-Wallis test, repeated-measures ANOVA or two-way ANOVA, and Tukey’s and Dunn’s post-hoc tests (α=0.05). The agar diffusion test showed a higher antibacterial property for 5% n-TiO2 compared with 3% and 7% (p<0.05) with no effect of time (1, 3, and 7 days). The cell number was significantly affected by all n-TiO2 groups, while viability was mostly affected by 3% and 5% n-TiO2, which also affected cell morphology and organization. Real-time PCR demonstrated that n-TiO2 reduced the expression of covR when compared with GIC with no n-TiO2 (p<0.05), with no effect of time, except for 3% n-TiO2 on vicR expression. Within-group and between-group analyses revealed n-TiO2 did not affect mRNA levels of gtfB, gtfC, and gtfD (p>0.05). Incorporation of n-TiO2 at 3% and 5% potentially affected S. mutans viability and the expression of key genes for bacterial survival and growth, improving the anticariogenic properties of GIC.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-14T10:31:44Z
2021-07-14T10:31:44Z
2021-06-14
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1807-3107bor-2021.vol35.0062
Brazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 35, p. -, 2021.
1806-8324
1807-3107
http://hdl.handle.net/11449/211923
10.1590/1807-3107bor-2021.vol35.0062
S1806-83242021000100253
S1806-83242021000100253.pdf
url http://dx.doi.org/10.1590/1807-3107bor-2021.vol35.0062
http://hdl.handle.net/11449/211923
identifier_str_mv Brazilian Oral Research. Sociedade Brasileira de Pesquisa Odontológica - SBPqO, v. 35, p. -, 2021.
1806-8324
1807-3107
10.1590/1807-3107bor-2021.vol35.0062
S1806-83242021000100253
S1806-83242021000100253.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Oral Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv -
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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