Omega-3 Fatty Acids Alter Systemic Inflammatory Mediators Caused by Apical Periodontitis

Detalhes bibliográficos
Autor(a) principal: Azuma, Mariane Maffei [UNESP]
Data de Publicação: 2021
Outros Autores: Cardoso, Carolina de Barros Morais [UNESP], Samuel, Renata Oliveira [UNESP], Pipa, Camila Barbosa [UNESP], Bomfim, Suely Regina Mogami [UNESP], Narciso, Luis Gustavo [UNESP], Gomes-Filho, João Eduardo [UNESP], Cintra, Luciano Tavares Angelo [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.joen.2020.11.015
http://hdl.handle.net/11449/207021
Resumo: Introduction: This study investigated the effects of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) on serum inflammatory mediators of rats with pulp exposure–induced apical periodontitis. Methods: Forty male Wistar rats were divided into the following groups: control, untreated rats (C); control rats treated with ω-3 PUFAs (C-O); rats with pulp exposure–induced apical periodontitis (AP); and rats with pulp exposure–induced apical periodontitis treated with ω-3 PUFAs (AP-O). ω-3 PUFAs were administered orally once a day for 15 days before pulp exposure and continued for 30 days after pulp exposure. The rats were sacrificed, and then blood and jaw samples were collected. Blood analysis was conducted to determine the total number of leukocytes including neutrophils, monocytes, and lymphocytes. Proinflammatory cytokines tumor necrosis factor alpha, interleukin (IL) 6, and IL-17 were quantified by enzyme-linked immunosorbent assay. Histologic analysis was performed to confirm the development of apical periodontitis. The data were statistically evaluated using analysis of variance and the Tukey posttest. The significance level was set at 5%. Results: The development of apical periodontitis was confirmed in all infected groups. Bone destruction was larger in the AP group compared with the AP-O group (P <.05). Blood analysis showed that the AP and AP-O groups showed higher numbers of lymphocytes, leukocytes, monocytes, eosinophils, and expressions of tumor necrosis factor alpha and IL-6 compared with the C and C-O groups (P <.05). In contrast, the presence of leukocytes, lymphocytes, and the expression of IL-6 decreased in the AP-O group compared with the AP group (P <.05). Conclusions: ω-3 PUFA supplementation influences the systemic effects caused by apical periodontitis, decreasing the number of leukocytes, lymphocytes, and IL-6 in rat blood.
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spelling Omega-3 Fatty Acids Alter Systemic Inflammatory Mediators Caused by Apical PeriodontitisApical periodontitisendodontic infectionendodontic medicineomega-3 fatty acidsIntroduction: This study investigated the effects of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) on serum inflammatory mediators of rats with pulp exposure–induced apical periodontitis. Methods: Forty male Wistar rats were divided into the following groups: control, untreated rats (C); control rats treated with ω-3 PUFAs (C-O); rats with pulp exposure–induced apical periodontitis (AP); and rats with pulp exposure–induced apical periodontitis treated with ω-3 PUFAs (AP-O). ω-3 PUFAs were administered orally once a day for 15 days before pulp exposure and continued for 30 days after pulp exposure. The rats were sacrificed, and then blood and jaw samples were collected. Blood analysis was conducted to determine the total number of leukocytes including neutrophils, monocytes, and lymphocytes. Proinflammatory cytokines tumor necrosis factor alpha, interleukin (IL) 6, and IL-17 were quantified by enzyme-linked immunosorbent assay. Histologic analysis was performed to confirm the development of apical periodontitis. The data were statistically evaluated using analysis of variance and the Tukey posttest. The significance level was set at 5%. Results: The development of apical periodontitis was confirmed in all infected groups. Bone destruction was larger in the AP group compared with the AP-O group (P <.05). Blood analysis showed that the AP and AP-O groups showed higher numbers of lymphocytes, leukocytes, monocytes, eosinophils, and expressions of tumor necrosis factor alpha and IL-6 compared with the C and C-O groups (P <.05). In contrast, the presence of leukocytes, lymphocytes, and the expression of IL-6 decreased in the AP-O group compared with the AP group (P <.05). Conclusions: ω-3 PUFA supplementation influences the systemic effects caused by apical periodontitis, decreasing the number of leukocytes, lymphocytes, and IL-6 in rat blood.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (Unesp)Department of Cariology Restorative Sciences and Endodontics University of MichiganDepartment of Clinical Dentistry Dental School University of UberabaDepartment of Clinic and Surgery and Animal Reproduction São Paulo State University (Unesp) Araçatuba Veterinary MedicineDepartment of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (Unesp)Department of Clinic and Surgery and Animal Reproduction São Paulo State University (Unesp) Araçatuba Veterinary MedicineFAPESP: 2013/26390-0FAPESP: 2015/03054-0Universidade Estadual Paulista (Unesp)University of MichiganUniversity of UberabaAzuma, Mariane Maffei [UNESP]Cardoso, Carolina de Barros Morais [UNESP]Samuel, Renata Oliveira [UNESP]Pipa, Camila Barbosa [UNESP]Bomfim, Suely Regina Mogami [UNESP]Narciso, Luis Gustavo [UNESP]Gomes-Filho, João Eduardo [UNESP]Cintra, Luciano Tavares Angelo [UNESP]2021-06-25T10:47:43Z2021-06-25T10:47:43Z2021-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article272-277http://dx.doi.org/10.1016/j.joen.2020.11.015Journal of Endodontics, v. 47, n. 2, p. 272-277, 2021.0099-2399http://hdl.handle.net/11449/20702110.1016/j.joen.2020.11.0152-s2.0-85098210660Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Endodonticsinfo:eu-repo/semantics/openAccess2024-09-04T18:03:59Zoai:repositorio.unesp.br:11449/207021Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:03:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Omega-3 Fatty Acids Alter Systemic Inflammatory Mediators Caused by Apical Periodontitis
title Omega-3 Fatty Acids Alter Systemic Inflammatory Mediators Caused by Apical Periodontitis
spellingShingle Omega-3 Fatty Acids Alter Systemic Inflammatory Mediators Caused by Apical Periodontitis
Azuma, Mariane Maffei [UNESP]
Apical periodontitis
endodontic infection
endodontic medicine
omega-3 fatty acids
title_short Omega-3 Fatty Acids Alter Systemic Inflammatory Mediators Caused by Apical Periodontitis
title_full Omega-3 Fatty Acids Alter Systemic Inflammatory Mediators Caused by Apical Periodontitis
title_fullStr Omega-3 Fatty Acids Alter Systemic Inflammatory Mediators Caused by Apical Periodontitis
title_full_unstemmed Omega-3 Fatty Acids Alter Systemic Inflammatory Mediators Caused by Apical Periodontitis
title_sort Omega-3 Fatty Acids Alter Systemic Inflammatory Mediators Caused by Apical Periodontitis
author Azuma, Mariane Maffei [UNESP]
author_facet Azuma, Mariane Maffei [UNESP]
Cardoso, Carolina de Barros Morais [UNESP]
Samuel, Renata Oliveira [UNESP]
Pipa, Camila Barbosa [UNESP]
Bomfim, Suely Regina Mogami [UNESP]
Narciso, Luis Gustavo [UNESP]
Gomes-Filho, João Eduardo [UNESP]
Cintra, Luciano Tavares Angelo [UNESP]
author_role author
author2 Cardoso, Carolina de Barros Morais [UNESP]
Samuel, Renata Oliveira [UNESP]
Pipa, Camila Barbosa [UNESP]
Bomfim, Suely Regina Mogami [UNESP]
Narciso, Luis Gustavo [UNESP]
Gomes-Filho, João Eduardo [UNESP]
Cintra, Luciano Tavares Angelo [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
University of Michigan
University of Uberaba
dc.contributor.author.fl_str_mv Azuma, Mariane Maffei [UNESP]
Cardoso, Carolina de Barros Morais [UNESP]
Samuel, Renata Oliveira [UNESP]
Pipa, Camila Barbosa [UNESP]
Bomfim, Suely Regina Mogami [UNESP]
Narciso, Luis Gustavo [UNESP]
Gomes-Filho, João Eduardo [UNESP]
Cintra, Luciano Tavares Angelo [UNESP]
dc.subject.por.fl_str_mv Apical periodontitis
endodontic infection
endodontic medicine
omega-3 fatty acids
topic Apical periodontitis
endodontic infection
endodontic medicine
omega-3 fatty acids
description Introduction: This study investigated the effects of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) on serum inflammatory mediators of rats with pulp exposure–induced apical periodontitis. Methods: Forty male Wistar rats were divided into the following groups: control, untreated rats (C); control rats treated with ω-3 PUFAs (C-O); rats with pulp exposure–induced apical periodontitis (AP); and rats with pulp exposure–induced apical periodontitis treated with ω-3 PUFAs (AP-O). ω-3 PUFAs were administered orally once a day for 15 days before pulp exposure and continued for 30 days after pulp exposure. The rats were sacrificed, and then blood and jaw samples were collected. Blood analysis was conducted to determine the total number of leukocytes including neutrophils, monocytes, and lymphocytes. Proinflammatory cytokines tumor necrosis factor alpha, interleukin (IL) 6, and IL-17 were quantified by enzyme-linked immunosorbent assay. Histologic analysis was performed to confirm the development of apical periodontitis. The data were statistically evaluated using analysis of variance and the Tukey posttest. The significance level was set at 5%. Results: The development of apical periodontitis was confirmed in all infected groups. Bone destruction was larger in the AP group compared with the AP-O group (P <.05). Blood analysis showed that the AP and AP-O groups showed higher numbers of lymphocytes, leukocytes, monocytes, eosinophils, and expressions of tumor necrosis factor alpha and IL-6 compared with the C and C-O groups (P <.05). In contrast, the presence of leukocytes, lymphocytes, and the expression of IL-6 decreased in the AP-O group compared with the AP group (P <.05). Conclusions: ω-3 PUFA supplementation influences the systemic effects caused by apical periodontitis, decreasing the number of leukocytes, lymphocytes, and IL-6 in rat blood.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:47:43Z
2021-06-25T10:47:43Z
2021-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.joen.2020.11.015
Journal of Endodontics, v. 47, n. 2, p. 272-277, 2021.
0099-2399
http://hdl.handle.net/11449/207021
10.1016/j.joen.2020.11.015
2-s2.0-85098210660
url http://dx.doi.org/10.1016/j.joen.2020.11.015
http://hdl.handle.net/11449/207021
identifier_str_mv Journal of Endodontics, v. 47, n. 2, p. 272-277, 2021.
0099-2399
10.1016/j.joen.2020.11.015
2-s2.0-85098210660
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Endodontics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 272-277
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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