First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii

Detalhes bibliográficos
Autor(a) principal: Wenceslau, Jose F. C. [UNESP]
Data de Publicação: 2022
Outros Autores: Santos, Eduardo F. [UNESP], Carnimeo, Fernando H. [UNESP], Noll, Fernando B. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/237704
Resumo: Spittlebugs cause major pasture damage in the Neotropics. As most xylem-feeders, they depend on microbial symbionts to supply essential amino acids to their diet. Here, the obligate nutritional endosymbiont `Candidatus Sulcia muelleri' (Bacteroidetes) was detected in three main cercopid pests of South America: Notozulia entreriana (Berg), Deois (Deois) mourei Cavichioli et Sakakibara and Deois (Deois) knoblauchii (Berg) (Cercopidae Ishnorhininae). In all insect species, bacteriomes were located laterally in the abdomen, and ultrathin sections of N. entreriana bacteriocytes showed typical sulcia-like bacteria. PCR and sequencing of a 914-bp fragment of the bacterial 16S rRNA gene revealed 100% nucleotide identity among sulcia strains obtained from the three host species. These sequences were also identical to those previously obtained from two other New World spittlebugs of the same subfamily, providing evidence for host/symbiont coevolution. Microscopic and molecular analyses suggested that N. entreriana lacked additional symbionts (i. e. 'Candidatus Zinderia insecticola' or sodalis-like bacteria [Proteobacteria]) that often co-occur with sulcia within members of the superfamily Cercopoidea. Though amplicons were occasionally generated from D. (D.) mourei and D. (D.) knoblauchii with primers intended for zinderia, they failed to sequence. Further research is needed to elucidate the identity of bacteria other than sulcia in Deois spp.
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spelling First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchiiCercopidaeBacteriome'Candidatus Sulcia muelleri'PhylogenySouth AmericaSpittlebugs cause major pasture damage in the Neotropics. As most xylem-feeders, they depend on microbial symbionts to supply essential amino acids to their diet. Here, the obligate nutritional endosymbiont `Candidatus Sulcia muelleri' (Bacteroidetes) was detected in three main cercopid pests of South America: Notozulia entreriana (Berg), Deois (Deois) mourei Cavichioli et Sakakibara and Deois (Deois) knoblauchii (Berg) (Cercopidae Ishnorhininae). In all insect species, bacteriomes were located laterally in the abdomen, and ultrathin sections of N. entreriana bacteriocytes showed typical sulcia-like bacteria. PCR and sequencing of a 914-bp fragment of the bacterial 16S rRNA gene revealed 100% nucleotide identity among sulcia strains obtained from the three host species. These sequences were also identical to those previously obtained from two other New World spittlebugs of the same subfamily, providing evidence for host/symbiont coevolution. Microscopic and molecular analyses suggested that N. entreriana lacked additional symbionts (i. e. 'Candidatus Zinderia insecticola' or sodalis-like bacteria [Proteobacteria]) that often co-occur with sulcia within members of the superfamily Cercopoidea. Though amplicons were occasionally generated from D. (D.) mourei and D. (D.) knoblauchii with primers intended for zinderia, they failed to sequence. Further research is needed to elucidate the identity of bacteria other than sulcia in Deois spp.Secretaria de Agricultura e Abastecimento do governo do Estado de Sao PauloUniv Sao Paulo Unesp, Dept Zool & Bot, Lab Aculeata, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP, BrazilUniv Sao Paulo Unesp, Dept Zool & Bot, Lab Aculeata, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP, BrazilAlma Mater Studiorum, Univ BolognaUniversidade Estadual Paulista (UNESP)Wenceslau, Jose F. C. [UNESP]Santos, Eduardo F. [UNESP]Carnimeo, Fernando H. [UNESP]Noll, Fernando B. [UNESP]2022-11-30T13:42:28Z2022-11-30T13:42:28Z2022-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article160Bulletin Of Insectology. Bologna: Alma Mater Studiorum, Univ Bologna, v. 75, n. 1, 160 p., 2022.1721-8861http://hdl.handle.net/11449/237704WOS:000778777700006Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBulletin Of Insectologyinfo:eu-repo/semantics/openAccess2022-11-30T13:42:29Zoai:repositorio.unesp.br:11449/237704Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:58:38.702590Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii
title First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii
spellingShingle First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii
Wenceslau, Jose F. C. [UNESP]
Cercopidae
Bacteriome
'Candidatus Sulcia muelleri'
Phylogeny
South America
title_short First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii
title_full First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii
title_fullStr First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii
title_full_unstemmed First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii
title_sort First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii
author Wenceslau, Jose F. C. [UNESP]
author_facet Wenceslau, Jose F. C. [UNESP]
Santos, Eduardo F. [UNESP]
Carnimeo, Fernando H. [UNESP]
Noll, Fernando B. [UNESP]
author_role author
author2 Santos, Eduardo F. [UNESP]
Carnimeo, Fernando H. [UNESP]
Noll, Fernando B. [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Wenceslau, Jose F. C. [UNESP]
Santos, Eduardo F. [UNESP]
Carnimeo, Fernando H. [UNESP]
Noll, Fernando B. [UNESP]
dc.subject.por.fl_str_mv Cercopidae
Bacteriome
'Candidatus Sulcia muelleri'
Phylogeny
South America
topic Cercopidae
Bacteriome
'Candidatus Sulcia muelleri'
Phylogeny
South America
description Spittlebugs cause major pasture damage in the Neotropics. As most xylem-feeders, they depend on microbial symbionts to supply essential amino acids to their diet. Here, the obligate nutritional endosymbiont `Candidatus Sulcia muelleri' (Bacteroidetes) was detected in three main cercopid pests of South America: Notozulia entreriana (Berg), Deois (Deois) mourei Cavichioli et Sakakibara and Deois (Deois) knoblauchii (Berg) (Cercopidae Ishnorhininae). In all insect species, bacteriomes were located laterally in the abdomen, and ultrathin sections of N. entreriana bacteriocytes showed typical sulcia-like bacteria. PCR and sequencing of a 914-bp fragment of the bacterial 16S rRNA gene revealed 100% nucleotide identity among sulcia strains obtained from the three host species. These sequences were also identical to those previously obtained from two other New World spittlebugs of the same subfamily, providing evidence for host/symbiont coevolution. Microscopic and molecular analyses suggested that N. entreriana lacked additional symbionts (i. e. 'Candidatus Zinderia insecticola' or sodalis-like bacteria [Proteobacteria]) that often co-occur with sulcia within members of the superfamily Cercopoidea. Though amplicons were occasionally generated from D. (D.) mourei and D. (D.) knoblauchii with primers intended for zinderia, they failed to sequence. Further research is needed to elucidate the identity of bacteria other than sulcia in Deois spp.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-30T13:42:28Z
2022-11-30T13:42:28Z
2022-06-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Bulletin Of Insectology. Bologna: Alma Mater Studiorum, Univ Bologna, v. 75, n. 1, 160 p., 2022.
1721-8861
http://hdl.handle.net/11449/237704
WOS:000778777700006
identifier_str_mv Bulletin Of Insectology. Bologna: Alma Mater Studiorum, Univ Bologna, v. 75, n. 1, 160 p., 2022.
1721-8861
WOS:000778777700006
url http://hdl.handle.net/11449/237704
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bulletin Of Insectology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 160
dc.publisher.none.fl_str_mv Alma Mater Studiorum, Univ Bologna
publisher.none.fl_str_mv Alma Mater Studiorum, Univ Bologna
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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