First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/237704 |
Resumo: | Spittlebugs cause major pasture damage in the Neotropics. As most xylem-feeders, they depend on microbial symbionts to supply essential amino acids to their diet. Here, the obligate nutritional endosymbiont `Candidatus Sulcia muelleri' (Bacteroidetes) was detected in three main cercopid pests of South America: Notozulia entreriana (Berg), Deois (Deois) mourei Cavichioli et Sakakibara and Deois (Deois) knoblauchii (Berg) (Cercopidae Ishnorhininae). In all insect species, bacteriomes were located laterally in the abdomen, and ultrathin sections of N. entreriana bacteriocytes showed typical sulcia-like bacteria. PCR and sequencing of a 914-bp fragment of the bacterial 16S rRNA gene revealed 100% nucleotide identity among sulcia strains obtained from the three host species. These sequences were also identical to those previously obtained from two other New World spittlebugs of the same subfamily, providing evidence for host/symbiont coevolution. Microscopic and molecular analyses suggested that N. entreriana lacked additional symbionts (i. e. 'Candidatus Zinderia insecticola' or sodalis-like bacteria [Proteobacteria]) that often co-occur with sulcia within members of the superfamily Cercopoidea. Though amplicons were occasionally generated from D. (D.) mourei and D. (D.) knoblauchii with primers intended for zinderia, they failed to sequence. Further research is needed to elucidate the identity of bacteria other than sulcia in Deois spp. |
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First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchiiCercopidaeBacteriome'Candidatus Sulcia muelleri'PhylogenySouth AmericaSpittlebugs cause major pasture damage in the Neotropics. As most xylem-feeders, they depend on microbial symbionts to supply essential amino acids to their diet. Here, the obligate nutritional endosymbiont `Candidatus Sulcia muelleri' (Bacteroidetes) was detected in three main cercopid pests of South America: Notozulia entreriana (Berg), Deois (Deois) mourei Cavichioli et Sakakibara and Deois (Deois) knoblauchii (Berg) (Cercopidae Ishnorhininae). In all insect species, bacteriomes were located laterally in the abdomen, and ultrathin sections of N. entreriana bacteriocytes showed typical sulcia-like bacteria. PCR and sequencing of a 914-bp fragment of the bacterial 16S rRNA gene revealed 100% nucleotide identity among sulcia strains obtained from the three host species. These sequences were also identical to those previously obtained from two other New World spittlebugs of the same subfamily, providing evidence for host/symbiont coevolution. Microscopic and molecular analyses suggested that N. entreriana lacked additional symbionts (i. e. 'Candidatus Zinderia insecticola' or sodalis-like bacteria [Proteobacteria]) that often co-occur with sulcia within members of the superfamily Cercopoidea. Though amplicons were occasionally generated from D. (D.) mourei and D. (D.) knoblauchii with primers intended for zinderia, they failed to sequence. Further research is needed to elucidate the identity of bacteria other than sulcia in Deois spp.Secretaria de Agricultura e Abastecimento do governo do Estado de Sao PauloUniv Sao Paulo Unesp, Dept Zool & Bot, Lab Aculeata, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP, BrazilUniv Sao Paulo Unesp, Dept Zool & Bot, Lab Aculeata, Rua Cristovao Colombo 2265, BR-15054000 Sao Jose Do Rio Preto, SP, BrazilAlma Mater Studiorum, Univ BolognaUniversidade Estadual Paulista (UNESP)Wenceslau, Jose F. C. [UNESP]Santos, Eduardo F. [UNESP]Carnimeo, Fernando H. [UNESP]Noll, Fernando B. [UNESP]2022-11-30T13:42:28Z2022-11-30T13:42:28Z2022-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article160Bulletin Of Insectology. Bologna: Alma Mater Studiorum, Univ Bologna, v. 75, n. 1, 160 p., 2022.1721-8861http://hdl.handle.net/11449/237704WOS:000778777700006Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBulletin Of Insectologyinfo:eu-repo/semantics/openAccess2022-11-30T13:42:29Zoai:repositorio.unesp.br:11449/237704Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:58:38.702590Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii |
title |
First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii |
spellingShingle |
First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii Wenceslau, Jose F. C. [UNESP] Cercopidae Bacteriome 'Candidatus Sulcia muelleri' Phylogeny South America |
title_short |
First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii |
title_full |
First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii |
title_fullStr |
First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii |
title_full_unstemmed |
First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii |
title_sort |
First identification of bacterial endosymbionts in three South- American spittlebug pests: Notozulia entreriana, Deois mourei and Deois knoblauchii |
author |
Wenceslau, Jose F. C. [UNESP] |
author_facet |
Wenceslau, Jose F. C. [UNESP] Santos, Eduardo F. [UNESP] Carnimeo, Fernando H. [UNESP] Noll, Fernando B. [UNESP] |
author_role |
author |
author2 |
Santos, Eduardo F. [UNESP] Carnimeo, Fernando H. [UNESP] Noll, Fernando B. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Wenceslau, Jose F. C. [UNESP] Santos, Eduardo F. [UNESP] Carnimeo, Fernando H. [UNESP] Noll, Fernando B. [UNESP] |
dc.subject.por.fl_str_mv |
Cercopidae Bacteriome 'Candidatus Sulcia muelleri' Phylogeny South America |
topic |
Cercopidae Bacteriome 'Candidatus Sulcia muelleri' Phylogeny South America |
description |
Spittlebugs cause major pasture damage in the Neotropics. As most xylem-feeders, they depend on microbial symbionts to supply essential amino acids to their diet. Here, the obligate nutritional endosymbiont `Candidatus Sulcia muelleri' (Bacteroidetes) was detected in three main cercopid pests of South America: Notozulia entreriana (Berg), Deois (Deois) mourei Cavichioli et Sakakibara and Deois (Deois) knoblauchii (Berg) (Cercopidae Ishnorhininae). In all insect species, bacteriomes were located laterally in the abdomen, and ultrathin sections of N. entreriana bacteriocytes showed typical sulcia-like bacteria. PCR and sequencing of a 914-bp fragment of the bacterial 16S rRNA gene revealed 100% nucleotide identity among sulcia strains obtained from the three host species. These sequences were also identical to those previously obtained from two other New World spittlebugs of the same subfamily, providing evidence for host/symbiont coevolution. Microscopic and molecular analyses suggested that N. entreriana lacked additional symbionts (i. e. 'Candidatus Zinderia insecticola' or sodalis-like bacteria [Proteobacteria]) that often co-occur with sulcia within members of the superfamily Cercopoidea. Though amplicons were occasionally generated from D. (D.) mourei and D. (D.) knoblauchii with primers intended for zinderia, they failed to sequence. Further research is needed to elucidate the identity of bacteria other than sulcia in Deois spp. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-30T13:42:28Z 2022-11-30T13:42:28Z 2022-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Bulletin Of Insectology. Bologna: Alma Mater Studiorum, Univ Bologna, v. 75, n. 1, 160 p., 2022. 1721-8861 http://hdl.handle.net/11449/237704 WOS:000778777700006 |
identifier_str_mv |
Bulletin Of Insectology. Bologna: Alma Mater Studiorum, Univ Bologna, v. 75, n. 1, 160 p., 2022. 1721-8861 WOS:000778777700006 |
url |
http://hdl.handle.net/11449/237704 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bulletin Of Insectology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
160 |
dc.publisher.none.fl_str_mv |
Alma Mater Studiorum, Univ Bologna |
publisher.none.fl_str_mv |
Alma Mater Studiorum, Univ Bologna |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128588951584768 |