High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-017-12939-7 http://hdl.handle.net/11449/175324 |
Resumo: | The high-throughput analysis of satellite DNA (satDNA) content, by means of Illumina sequencing, unveiled 45 satDNA families in the genome of Astyanax paranae, with repeat unit length (RUL) ranging from 6 to 365 bp and marked predominance of short satellites (median length = 59 bp). The analysis of chromosomal location of 35 satDNAs in A. paranae, A. fasciatus and A. bockmanni revealed that most satellites are shared between the three species and show highly similar patterns of chromosome distribution. The high similarity in satellite DNA content between these species is most likely due to their recent common descent. Among the few differences found, the ApaSat44-21 satellite was present only on the B chromosome of A. paranae, but not on the A or B chromosomes of the two other species. Likewise, the ApaSat20-18 satellite was B-specific in A. paranae but was however present on A and B chromosomes of A. fasciatus and A. bockmanni. The isochromosome nature of B chromosomes in these species was evidenced by the symmetric location of many satDNAs on both B chromosome arms, and the lower symmetry observed in the A. fasciatus BfMa chromosome suggests that it is older than those analyzed in A. paranae and A. bockmanni. |
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High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus AstyanaxThe high-throughput analysis of satellite DNA (satDNA) content, by means of Illumina sequencing, unveiled 45 satDNA families in the genome of Astyanax paranae, with repeat unit length (RUL) ranging from 6 to 365 bp and marked predominance of short satellites (median length = 59 bp). The analysis of chromosomal location of 35 satDNAs in A. paranae, A. fasciatus and A. bockmanni revealed that most satellites are shared between the three species and show highly similar patterns of chromosome distribution. The high similarity in satellite DNA content between these species is most likely due to their recent common descent. Among the few differences found, the ApaSat44-21 satellite was present only on the B chromosome of A. paranae, but not on the A or B chromosomes of the two other species. Likewise, the ApaSat20-18 satellite was B-specific in A. paranae but was however present on A and B chromosomes of A. fasciatus and A. bockmanni. The isochromosome nature of B chromosomes in these species was evidenced by the symmetric location of many satDNAs on both B chromosome arms, and the lower symmetry observed in the A. fasciatus BfMa chromosome suggests that it is older than those analyzed in A. paranae and A. bockmanni.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Departamento de Morfologia Instituto de Biociências Universidade Estadual Paulista - UNESP, Distrito de Rubião Junior, s/nDepartamento de Genética Universidad de GranadaDepartamento de Ciências Biológicas Faculdade de Ciências Universidade Estadual Paulista - UNESP Campus de BauruCAUNESP Universidade Estadual Paulista - UNESP Campus JaboticabalDepartamento de Morfologia Instituto de Biociências Universidade Estadual Paulista - UNESP, Distrito de Rubião Junior, s/nDepartamento de Ciências Biológicas Faculdade de Ciências Universidade Estadual Paulista - UNESP Campus de BauruCAUNESP Universidade Estadual Paulista - UNESP Campus JaboticabalFAPESP: 2013/24367-0FAPESP: 2014/09634-5CNPq: 448012/2014-6Universidade Estadual Paulista (Unesp)Universidad de GranadaDe Silva, Duílio M.Z.A. [UNESP]Utsunomia, Ricardo [UNESP]Ruiz-Ruano, Francisco J.Daniel, Sandro Natal [UNESP]Porto-Foresti, Fábio [UNESP]Hashimoto, Diogo Teruo [UNESP]Oliveira, Claudio [UNESP]Camacho, Juan Pedro M.Foresti, Fausto [UNESP]2018-12-11T17:15:18Z2018-12-11T17:15:18Z2017-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1038/s41598-017-12939-7Scientific Reports, v. 7, n. 1, 2017.2045-2322http://hdl.handle.net/11449/17532410.1038/s41598-017-12939-72-s2.0-850309737002-s2.0-85030973700.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reports1,533info:eu-repo/semantics/openAccess2024-04-23T15:23:16Zoai:repositorio.unesp.br:11449/175324Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-23T15:23:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax |
title |
High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax |
spellingShingle |
High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax De Silva, Duílio M.Z.A. [UNESP] |
title_short |
High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax |
title_full |
High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax |
title_fullStr |
High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax |
title_full_unstemmed |
High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax |
title_sort |
High-throughput analysis unveils a highly shared satellite DNA library among three species of fish genus Astyanax |
author |
De Silva, Duílio M.Z.A. [UNESP] |
author_facet |
De Silva, Duílio M.Z.A. [UNESP] Utsunomia, Ricardo [UNESP] Ruiz-Ruano, Francisco J. Daniel, Sandro Natal [UNESP] Porto-Foresti, Fábio [UNESP] Hashimoto, Diogo Teruo [UNESP] Oliveira, Claudio [UNESP] Camacho, Juan Pedro M. Foresti, Fausto [UNESP] |
author_role |
author |
author2 |
Utsunomia, Ricardo [UNESP] Ruiz-Ruano, Francisco J. Daniel, Sandro Natal [UNESP] Porto-Foresti, Fábio [UNESP] Hashimoto, Diogo Teruo [UNESP] Oliveira, Claudio [UNESP] Camacho, Juan Pedro M. Foresti, Fausto [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidad de Granada |
dc.contributor.author.fl_str_mv |
De Silva, Duílio M.Z.A. [UNESP] Utsunomia, Ricardo [UNESP] Ruiz-Ruano, Francisco J. Daniel, Sandro Natal [UNESP] Porto-Foresti, Fábio [UNESP] Hashimoto, Diogo Teruo [UNESP] Oliveira, Claudio [UNESP] Camacho, Juan Pedro M. Foresti, Fausto [UNESP] |
description |
The high-throughput analysis of satellite DNA (satDNA) content, by means of Illumina sequencing, unveiled 45 satDNA families in the genome of Astyanax paranae, with repeat unit length (RUL) ranging from 6 to 365 bp and marked predominance of short satellites (median length = 59 bp). The analysis of chromosomal location of 35 satDNAs in A. paranae, A. fasciatus and A. bockmanni revealed that most satellites are shared between the three species and show highly similar patterns of chromosome distribution. The high similarity in satellite DNA content between these species is most likely due to their recent common descent. Among the few differences found, the ApaSat44-21 satellite was present only on the B chromosome of A. paranae, but not on the A or B chromosomes of the two other species. Likewise, the ApaSat20-18 satellite was B-specific in A. paranae but was however present on A and B chromosomes of A. fasciatus and A. bockmanni. The isochromosome nature of B chromosomes in these species was evidenced by the symmetric location of many satDNAs on both B chromosome arms, and the lower symmetry observed in the A. fasciatus BfMa chromosome suggests that it is older than those analyzed in A. paranae and A. bockmanni. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-01 2018-12-11T17:15:18Z 2018-12-11T17:15:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-017-12939-7 Scientific Reports, v. 7, n. 1, 2017. 2045-2322 http://hdl.handle.net/11449/175324 10.1038/s41598-017-12939-7 2-s2.0-85030973700 2-s2.0-85030973700.pdf |
url |
http://dx.doi.org/10.1038/s41598-017-12939-7 http://hdl.handle.net/11449/175324 |
identifier_str_mv |
Scientific Reports, v. 7, n. 1, 2017. 2045-2322 10.1038/s41598-017-12939-7 2-s2.0-85030973700 2-s2.0-85030973700.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports 1,533 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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