Blockade of the mineralocorticoid receptors in the medial prefrontal cortex prevents the acquisition of one-trial tolerance in mice
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bbr.2022.113938 http://hdl.handle.net/11449/240202 |
Resumo: | One-trial tolerance (OTT) is characterized by the lack of anxiolytic-like effects of benzodiazepines in animals submitted to a trial 2 in the elevated plus-maze (EPM) and is described to be influenced by learning mechanisms. Mineralocorticoid receptors (MR) in the infralimbic subregion (IL) of the medial prefrontal cortex (mPFC) are important modulators of emotional learning, but the MR involvement in the establishment of OTT remains unclear. We investigated the effects of intra-IL infusions of RU 28318 (an MR antagonist) on the OTT to the anxiolytic effects of midazolam (MDZ, GABAA-benzodiazepine agonist) in mice exposed to a two-trial protocol in the EPM. First, mice were treated with saline or MDZ (2 mg/kg, i.p.) 30 min before trial 1 or 2 in the EPM, to characterize the OTT. To investigate the role of MR in the OTT, independent groups of mice received intra-IL infusions of vehicle or RU 28318 (5 or 10 ng/0.1 µL) immediately before or after first trial in the EPM. Twenty-four hours later, the same mice received injections of saline or MDZ and were re-tested in the EPM. The MDZ decreased anxiety-like behaviors in trial 1, but the same anxiolytic-like effect was not observed in MDZ-mice prior to the second EPM test, confirming the OTT. Blockade of MR in the IL before, but not after, trial 1 restored the anxiolytic effects if MDZ administered in trial 2. These findings indicate that the MR in the IL-mPFC contributing to the OTT by mediating the acquisition, but not the consolidation of emotional learning. |
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Blockade of the mineralocorticoid receptors in the medial prefrontal cortex prevents the acquisition of one-trial tolerance in miceAnxietyElevated plus mazeInfralimbicMemoryStressOne-trial tolerance (OTT) is characterized by the lack of anxiolytic-like effects of benzodiazepines in animals submitted to a trial 2 in the elevated plus-maze (EPM) and is described to be influenced by learning mechanisms. Mineralocorticoid receptors (MR) in the infralimbic subregion (IL) of the medial prefrontal cortex (mPFC) are important modulators of emotional learning, but the MR involvement in the establishment of OTT remains unclear. We investigated the effects of intra-IL infusions of RU 28318 (an MR antagonist) on the OTT to the anxiolytic effects of midazolam (MDZ, GABAA-benzodiazepine agonist) in mice exposed to a two-trial protocol in the EPM. First, mice were treated with saline or MDZ (2 mg/kg, i.p.) 30 min before trial 1 or 2 in the EPM, to characterize the OTT. To investigate the role of MR in the OTT, independent groups of mice received intra-IL infusions of vehicle or RU 28318 (5 or 10 ng/0.1 µL) immediately before or after first trial in the EPM. Twenty-four hours later, the same mice received injections of saline or MDZ and were re-tested in the EPM. The MDZ decreased anxiety-like behaviors in trial 1, but the same anxiolytic-like effect was not observed in MDZ-mice prior to the second EPM test, confirming the OTT. Blockade of MR in the IL before, but not after, trial 1 restored the anxiolytic effects if MDZ administered in trial 2. These findings indicate that the MR in the IL-mPFC contributing to the OTT by mediating the acquisition, but not the consolidation of emotional learning.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Psychobiology Group/Department of Psychology/CECH–UFSCar, SPJoint Graduate Program in Physiological Sciences UFSCar/UNESP, Rod. Washington Luís, Km 235The University of Texas at Dallas School of Behavior and Brain Sciences, 800 West Campbell RoadThe University of Texas at Dallas Texas Biomedical Device Center, 800 West Campbell RoadGraduate Program in Psychology UFSCar, Rod. Washington Luís, Km 235Neuroscience and Behavioral Institute, Av. do Café, 2.450Joint Graduate Program in Physiological Sciences UFSCar/UNESP, Rod. Washington Luís, Km 235CAPES: 001CNPq: 309201/2015-2CNPq: 451500/2013-0CNPq: 482356/2013-8Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (UNESP)School of Behavior and Brain SciencesTexas Biomedical Device CenterNeuroscience and Behavioral InstituteAlbernaz-Mariano, Kairo Alan [UNESP]Souza, Rimenez RodriguesCanto-de-Souza, Azair [UNESP]2023-03-01T20:06:10Z2023-03-01T20:06:10Z2022-08-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.bbr.2022.113938Behavioural Brain Research, v. 431.1872-75490166-4328http://hdl.handle.net/11449/24020210.1016/j.bbr.2022.1139382-s2.0-85131529741Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBehavioural Brain Researchinfo:eu-repo/semantics/openAccess2023-03-01T20:06:10Zoai:repositorio.unesp.br:11449/240202Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:47:58.682107Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Blockade of the mineralocorticoid receptors in the medial prefrontal cortex prevents the acquisition of one-trial tolerance in mice |
title |
Blockade of the mineralocorticoid receptors in the medial prefrontal cortex prevents the acquisition of one-trial tolerance in mice |
spellingShingle |
Blockade of the mineralocorticoid receptors in the medial prefrontal cortex prevents the acquisition of one-trial tolerance in mice Albernaz-Mariano, Kairo Alan [UNESP] Anxiety Elevated plus maze Infralimbic Memory Stress |
title_short |
Blockade of the mineralocorticoid receptors in the medial prefrontal cortex prevents the acquisition of one-trial tolerance in mice |
title_full |
Blockade of the mineralocorticoid receptors in the medial prefrontal cortex prevents the acquisition of one-trial tolerance in mice |
title_fullStr |
Blockade of the mineralocorticoid receptors in the medial prefrontal cortex prevents the acquisition of one-trial tolerance in mice |
title_full_unstemmed |
Blockade of the mineralocorticoid receptors in the medial prefrontal cortex prevents the acquisition of one-trial tolerance in mice |
title_sort |
Blockade of the mineralocorticoid receptors in the medial prefrontal cortex prevents the acquisition of one-trial tolerance in mice |
author |
Albernaz-Mariano, Kairo Alan [UNESP] |
author_facet |
Albernaz-Mariano, Kairo Alan [UNESP] Souza, Rimenez Rodrigues Canto-de-Souza, Azair [UNESP] |
author_role |
author |
author2 |
Souza, Rimenez Rodrigues Canto-de-Souza, Azair [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (UNESP) School of Behavior and Brain Sciences Texas Biomedical Device Center Neuroscience and Behavioral Institute |
dc.contributor.author.fl_str_mv |
Albernaz-Mariano, Kairo Alan [UNESP] Souza, Rimenez Rodrigues Canto-de-Souza, Azair [UNESP] |
dc.subject.por.fl_str_mv |
Anxiety Elevated plus maze Infralimbic Memory Stress |
topic |
Anxiety Elevated plus maze Infralimbic Memory Stress |
description |
One-trial tolerance (OTT) is characterized by the lack of anxiolytic-like effects of benzodiazepines in animals submitted to a trial 2 in the elevated plus-maze (EPM) and is described to be influenced by learning mechanisms. Mineralocorticoid receptors (MR) in the infralimbic subregion (IL) of the medial prefrontal cortex (mPFC) are important modulators of emotional learning, but the MR involvement in the establishment of OTT remains unclear. We investigated the effects of intra-IL infusions of RU 28318 (an MR antagonist) on the OTT to the anxiolytic effects of midazolam (MDZ, GABAA-benzodiazepine agonist) in mice exposed to a two-trial protocol in the EPM. First, mice were treated with saline or MDZ (2 mg/kg, i.p.) 30 min before trial 1 or 2 in the EPM, to characterize the OTT. To investigate the role of MR in the OTT, independent groups of mice received intra-IL infusions of vehicle or RU 28318 (5 or 10 ng/0.1 µL) immediately before or after first trial in the EPM. Twenty-four hours later, the same mice received injections of saline or MDZ and were re-tested in the EPM. The MDZ decreased anxiety-like behaviors in trial 1, but the same anxiolytic-like effect was not observed in MDZ-mice prior to the second EPM test, confirming the OTT. Blockade of MR in the IL before, but not after, trial 1 restored the anxiolytic effects if MDZ administered in trial 2. These findings indicate that the MR in the IL-mPFC contributing to the OTT by mediating the acquisition, but not the consolidation of emotional learning. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08-05 2023-03-01T20:06:10Z 2023-03-01T20:06:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bbr.2022.113938 Behavioural Brain Research, v. 431. 1872-7549 0166-4328 http://hdl.handle.net/11449/240202 10.1016/j.bbr.2022.113938 2-s2.0-85131529741 |
url |
http://dx.doi.org/10.1016/j.bbr.2022.113938 http://hdl.handle.net/11449/240202 |
identifier_str_mv |
Behavioural Brain Research, v. 431. 1872-7549 0166-4328 10.1016/j.bbr.2022.113938 2-s2.0-85131529741 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Behavioural Brain Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129359320449024 |