Three-dimensional visualization of human hemoglobin phenotypes with HPLC
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4238/vol8-1gmr530 http://hdl.handle.net/11449/71038 |
Resumo: | Hemoglobinopathies were included in the Brazilian Neonatal Screening Program on June 6, 2001. Automated high-performance liquid chromatography (HPLC) was indicated as one of the diagnostic methods. The amount of information generated by these systems is immense, and the behavior of groups cannot always be observed in individual analyses. Three-dimensional (3-D) visualization techniques can be applied to extract this information, for extracting patterns, trends or relations from the results stored in databases. We applied the 3-D visualization tool to analyze patterns in the results of hemoglobinopathy based on neonatal diagnosis by HPLC. The laboratory results of 2520 newborn analyses carried out in 2001 and 2002 were used. The Fast, F1, F and A peaks, which were detected by the analytical system, were chosen as attributes for mapping. To establish a behavior pattern, the results were classified into groups according to hemoglobin phenotype: normal (N = 2169), variant (N = 73) and thalassemia (N = 279). 3-D visualization was made with the FastMap DB tool; there were two distribution patterns in the normal group, due to variation in the amplitude of the values obtained by HPLC for the F1 window. It allowed separation of the samples with normal Hb from those with alpha thalassemia, based on a significant difference (P > 0.05) between the mean values of the Fast and A peaks, demonstrating the need for better evaluation of chromatograms; this method could be used to help diagnose alpha thalassemia in newborns. |
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Three-dimensional visualization of human hemoglobin phenotypes with HPLC3-d visualizationFastmap dbHemoglobinopathiesNeonatal screeninghemoglobinalpha thalassemiachromatographycontrolled studydata basedata mininghemoglobinopathyhigh performance liquid chromatographyhumanlaboratory testnewbornnewborn screeningphenotypethree dimensional imagingalpha-ThalassemiaChromatography, High Pressure LiquidHemoglobinsHumansImaging, Three-DimensionalInfant, NewbornNeonatal ScreeningPhenotypeSoftwareHemoglobinopathies were included in the Brazilian Neonatal Screening Program on June 6, 2001. Automated high-performance liquid chromatography (HPLC) was indicated as one of the diagnostic methods. The amount of information generated by these systems is immense, and the behavior of groups cannot always be observed in individual analyses. Three-dimensional (3-D) visualization techniques can be applied to extract this information, for extracting patterns, trends or relations from the results stored in databases. We applied the 3-D visualization tool to analyze patterns in the results of hemoglobinopathy based on neonatal diagnosis by HPLC. The laboratory results of 2520 newborn analyses carried out in 2001 and 2002 were used. The Fast, F1, F and A peaks, which were detected by the analytical system, were chosen as attributes for mapping. To establish a behavior pattern, the results were classified into groups according to hemoglobin phenotype: normal (N = 2169), variant (N = 73) and thalassemia (N = 279). 3-D visualization was made with the FastMap DB tool; there were two distribution patterns in the normal group, due to variation in the amplitude of the values obtained by HPLC for the F1 window. It allowed separation of the samples with normal Hb from those with alpha thalassemia, based on a significant difference (P > 0.05) between the mean values of the Fast and A peaks, demonstrating the need for better evaluation of chromatograms; this method could be used to help diagnose alpha thalassemia in newborns.Departamento de Biologia Universidade Estadual Paulista Julio de Mesquita Filho Instituto de Biociencias, Letras e Ciencias Exatas, Sao Jose do Rio Preto, SPCentro de Investigacao de Microorganismos Faculdade de Medicina de Sao Jose do Rio Preto, Sao Jose do Rio Preto, SPDepartamento de Estatistica e Ciencias da Computacao Universidade Estadual Paulista Julio de Mesquita Filho Universidade Estadual Paulista, Letras e Ciencias Exatas, Sao Jose do Rio Preto, SPDepartamento de Ciencia da Computacao Universidade de Sao Paulo, Sao Paulo, SPDepartamento de Biologia Universidade Estadual Paulista Julio de Mesquita Filho Instituto de Biociencias, Letras e Ciencias Exatas, Sao Jose do Rio Preto, SPDepartamento de Estatistica e Ciencias da Computacao Universidade Estadual Paulista Julio de Mesquita Filho Universidade Estadual Paulista, Letras e Ciencias Exatas, Sao Jose do Rio Preto, SPUniversidade Estadual Paulista (Unesp)Faculdade de Medicina de São José do Rio Preto (FAMERP)Universidade de São Paulo (USP)Storti-Melo, L. M. [UNESP]Mangonaro, P. H. [UNESP]Valencio, C. R. [UNESP]Traina Junior, C.Domingos, C. R B [UNESP]2014-05-27T11:23:55Z2014-05-27T11:23:55Z2009-06-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article354-363application/pdfhttp://dx.doi.org/10.4238/vol8-1gmr530Genetics and Molecular Research, v. 8, n. 1, p. 354-363, 2009.1676-5680http://hdl.handle.net/11449/7103810.4238/vol8-1gmr5302-s2.0-667491038532-s2.0-66749103853.pdf3279428066176719Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Research0,439info:eu-repo/semantics/openAccess2023-12-16T06:23:37Zoai:repositorio.unesp.br:11449/71038Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-16T06:23:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Three-dimensional visualization of human hemoglobin phenotypes with HPLC |
title |
Three-dimensional visualization of human hemoglobin phenotypes with HPLC |
spellingShingle |
Three-dimensional visualization of human hemoglobin phenotypes with HPLC Storti-Melo, L. M. [UNESP] 3-d visualization Fastmap db Hemoglobinopathies Neonatal screening hemoglobin alpha thalassemia chromatography controlled study data base data mining hemoglobinopathy high performance liquid chromatography human laboratory test newborn newborn screening phenotype three dimensional imaging alpha-Thalassemia Chromatography, High Pressure Liquid Hemoglobins Humans Imaging, Three-Dimensional Infant, Newborn Neonatal Screening Phenotype Software |
title_short |
Three-dimensional visualization of human hemoglobin phenotypes with HPLC |
title_full |
Three-dimensional visualization of human hemoglobin phenotypes with HPLC |
title_fullStr |
Three-dimensional visualization of human hemoglobin phenotypes with HPLC |
title_full_unstemmed |
Three-dimensional visualization of human hemoglobin phenotypes with HPLC |
title_sort |
Three-dimensional visualization of human hemoglobin phenotypes with HPLC |
author |
Storti-Melo, L. M. [UNESP] |
author_facet |
Storti-Melo, L. M. [UNESP] Mangonaro, P. H. [UNESP] Valencio, C. R. [UNESP] Traina Junior, C. Domingos, C. R B [UNESP] |
author_role |
author |
author2 |
Mangonaro, P. H. [UNESP] Valencio, C. R. [UNESP] Traina Junior, C. Domingos, C. R B [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Faculdade de Medicina de São José do Rio Preto (FAMERP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Storti-Melo, L. M. [UNESP] Mangonaro, P. H. [UNESP] Valencio, C. R. [UNESP] Traina Junior, C. Domingos, C. R B [UNESP] |
dc.subject.por.fl_str_mv |
3-d visualization Fastmap db Hemoglobinopathies Neonatal screening hemoglobin alpha thalassemia chromatography controlled study data base data mining hemoglobinopathy high performance liquid chromatography human laboratory test newborn newborn screening phenotype three dimensional imaging alpha-Thalassemia Chromatography, High Pressure Liquid Hemoglobins Humans Imaging, Three-Dimensional Infant, Newborn Neonatal Screening Phenotype Software |
topic |
3-d visualization Fastmap db Hemoglobinopathies Neonatal screening hemoglobin alpha thalassemia chromatography controlled study data base data mining hemoglobinopathy high performance liquid chromatography human laboratory test newborn newborn screening phenotype three dimensional imaging alpha-Thalassemia Chromatography, High Pressure Liquid Hemoglobins Humans Imaging, Three-Dimensional Infant, Newborn Neonatal Screening Phenotype Software |
description |
Hemoglobinopathies were included in the Brazilian Neonatal Screening Program on June 6, 2001. Automated high-performance liquid chromatography (HPLC) was indicated as one of the diagnostic methods. The amount of information generated by these systems is immense, and the behavior of groups cannot always be observed in individual analyses. Three-dimensional (3-D) visualization techniques can be applied to extract this information, for extracting patterns, trends or relations from the results stored in databases. We applied the 3-D visualization tool to analyze patterns in the results of hemoglobinopathy based on neonatal diagnosis by HPLC. The laboratory results of 2520 newborn analyses carried out in 2001 and 2002 were used. The Fast, F1, F and A peaks, which were detected by the analytical system, were chosen as attributes for mapping. To establish a behavior pattern, the results were classified into groups according to hemoglobin phenotype: normal (N = 2169), variant (N = 73) and thalassemia (N = 279). 3-D visualization was made with the FastMap DB tool; there were two distribution patterns in the normal group, due to variation in the amplitude of the values obtained by HPLC for the F1 window. It allowed separation of the samples with normal Hb from those with alpha thalassemia, based on a significant difference (P > 0.05) between the mean values of the Fast and A peaks, demonstrating the need for better evaluation of chromatograms; this method could be used to help diagnose alpha thalassemia in newborns. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-06-15 2014-05-27T11:23:55Z 2014-05-27T11:23:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4238/vol8-1gmr530 Genetics and Molecular Research, v. 8, n. 1, p. 354-363, 2009. 1676-5680 http://hdl.handle.net/11449/71038 10.4238/vol8-1gmr530 2-s2.0-66749103853 2-s2.0-66749103853.pdf 3279428066176719 |
url |
http://dx.doi.org/10.4238/vol8-1gmr530 http://hdl.handle.net/11449/71038 |
identifier_str_mv |
Genetics and Molecular Research, v. 8, n. 1, p. 354-363, 2009. 1676-5680 10.4238/vol8-1gmr530 2-s2.0-66749103853 2-s2.0-66749103853.pdf 3279428066176719 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Research 0,439 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
354-363 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046978785902592 |