Thiamethoxam exposure deregulates short ORF gene expression in the honey bee and compromises immune response to bacteria
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-020-80620-7 http://hdl.handle.net/11449/208354 |
Resumo: | Maximizing crop yields relies on the use of agrochemicals to control insect pests. One of the most widely used classes of insecticides are neonicotinoids that interfere with signalling of the neurotransmitter acetylcholine, but these can also disrupt crop-pollination services provided by bees. Here, we analysed whether chronic low dose long-term exposure to the neonicotinoid thiamethoxam alters gene expression and alternative splicing in brains of Africanized honey bees, Apis mellifera, as adaptation to altered neuronal signalling. We find differentially regulated genes that show concentration-dependent responses to thiamethoxam, but no changes in alternative splicing. Most differentially expressed genes have no annotated function but encode short Open Reading Frames, a characteristic feature of anti-microbial peptides. As this suggested that immune responses may be compromised by thiamethoxam exposure, we tested the impact of thiamethoxam on bee immunity by injecting bacteria. We show that intrinsically sub-lethal thiamethoxam exposure makes bees more vulnerable to normally non-pathogenic bacteria. Our findings imply a synergistic mechanism for the observed bee population declines that concern agriculturists, conservation ecologists and the public. |
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Thiamethoxam exposure deregulates short ORF gene expression in the honey bee and compromises immune response to bacteriaMaximizing crop yields relies on the use of agrochemicals to control insect pests. One of the most widely used classes of insecticides are neonicotinoids that interfere with signalling of the neurotransmitter acetylcholine, but these can also disrupt crop-pollination services provided by bees. Here, we analysed whether chronic low dose long-term exposure to the neonicotinoid thiamethoxam alters gene expression and alternative splicing in brains of Africanized honey bees, Apis mellifera, as adaptation to altered neuronal signalling. We find differentially regulated genes that show concentration-dependent responses to thiamethoxam, but no changes in alternative splicing. Most differentially expressed genes have no annotated function but encode short Open Reading Frames, a characteristic feature of anti-microbial peptides. As this suggested that immune responses may be compromised by thiamethoxam exposure, we tested the impact of thiamethoxam on bee immunity by injecting bacteria. We show that intrinsically sub-lethal thiamethoxam exposure makes bees more vulnerable to normally non-pathogenic bacteria. Our findings imply a synergistic mechanism for the observed bee population declines that concern agriculturists, conservation ecologists and the public.Biotechnology and Biological Sciences Research CouncilFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Institute of Biosciences São Paulo State University (Unesp)School of Biosciences College of Life and Environmental Sciences University of Birmingham, EdgbastonSchool of Biosciences University of Nottingham, Sutton BoningtonSchool of Veterinary Medicine and Science University of Nottingham, Sutton BoningtonInstitute of Biosciences São Paulo State University (Unesp)Universidade Estadual Paulista (Unesp)University of BirminghamUniversity of NottinghamDecio, Pâmela [UNESP]Ustaoglu, PinarDerecka, KamilaHardy, Ian C. W.Roat, Thaisa C. [UNESP]Malaspina, Osmar [UNESP]Mongan, NigelStöger, ReinhardSoller, Matthias2021-06-25T11:10:45Z2021-06-25T11:10:45Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-020-80620-7Scientific Reports, v. 11, n. 1, 2021.2045-2322http://hdl.handle.net/11449/20835410.1038/s41598-020-80620-72-s2.0-85100076354Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2021-10-23T19:02:09Zoai:repositorio.unesp.br:11449/208354Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:20:46.721282Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Thiamethoxam exposure deregulates short ORF gene expression in the honey bee and compromises immune response to bacteria |
title |
Thiamethoxam exposure deregulates short ORF gene expression in the honey bee and compromises immune response to bacteria |
spellingShingle |
Thiamethoxam exposure deregulates short ORF gene expression in the honey bee and compromises immune response to bacteria Decio, Pâmela [UNESP] |
title_short |
Thiamethoxam exposure deregulates short ORF gene expression in the honey bee and compromises immune response to bacteria |
title_full |
Thiamethoxam exposure deregulates short ORF gene expression in the honey bee and compromises immune response to bacteria |
title_fullStr |
Thiamethoxam exposure deregulates short ORF gene expression in the honey bee and compromises immune response to bacteria |
title_full_unstemmed |
Thiamethoxam exposure deregulates short ORF gene expression in the honey bee and compromises immune response to bacteria |
title_sort |
Thiamethoxam exposure deregulates short ORF gene expression in the honey bee and compromises immune response to bacteria |
author |
Decio, Pâmela [UNESP] |
author_facet |
Decio, Pâmela [UNESP] Ustaoglu, Pinar Derecka, Kamila Hardy, Ian C. W. Roat, Thaisa C. [UNESP] Malaspina, Osmar [UNESP] Mongan, Nigel Stöger, Reinhard Soller, Matthias |
author_role |
author |
author2 |
Ustaoglu, Pinar Derecka, Kamila Hardy, Ian C. W. Roat, Thaisa C. [UNESP] Malaspina, Osmar [UNESP] Mongan, Nigel Stöger, Reinhard Soller, Matthias |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Birmingham University of Nottingham |
dc.contributor.author.fl_str_mv |
Decio, Pâmela [UNESP] Ustaoglu, Pinar Derecka, Kamila Hardy, Ian C. W. Roat, Thaisa C. [UNESP] Malaspina, Osmar [UNESP] Mongan, Nigel Stöger, Reinhard Soller, Matthias |
description |
Maximizing crop yields relies on the use of agrochemicals to control insect pests. One of the most widely used classes of insecticides are neonicotinoids that interfere with signalling of the neurotransmitter acetylcholine, but these can also disrupt crop-pollination services provided by bees. Here, we analysed whether chronic low dose long-term exposure to the neonicotinoid thiamethoxam alters gene expression and alternative splicing in brains of Africanized honey bees, Apis mellifera, as adaptation to altered neuronal signalling. We find differentially regulated genes that show concentration-dependent responses to thiamethoxam, but no changes in alternative splicing. Most differentially expressed genes have no annotated function but encode short Open Reading Frames, a characteristic feature of anti-microbial peptides. As this suggested that immune responses may be compromised by thiamethoxam exposure, we tested the impact of thiamethoxam on bee immunity by injecting bacteria. We show that intrinsically sub-lethal thiamethoxam exposure makes bees more vulnerable to normally non-pathogenic bacteria. Our findings imply a synergistic mechanism for the observed bee population declines that concern agriculturists, conservation ecologists and the public. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:10:45Z 2021-06-25T11:10:45Z 2021-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-020-80620-7 Scientific Reports, v. 11, n. 1, 2021. 2045-2322 http://hdl.handle.net/11449/208354 10.1038/s41598-020-80620-7 2-s2.0-85100076354 |
url |
http://dx.doi.org/10.1038/s41598-020-80620-7 http://hdl.handle.net/11449/208354 |
identifier_str_mv |
Scientific Reports, v. 11, n. 1, 2021. 2045-2322 10.1038/s41598-020-80620-7 2-s2.0-85100076354 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129417681043456 |