Progesterone (PR), Oestrogen (ER-alpha and ER-beta) and Oxytocin (OTR) Gene Expression in the Oviduct and Uterus of Pregnant and Non-pregnant Bitches
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/rda.12015 http://hdl.handle.net/11449/40919 |
Resumo: | Contents The aim was to assess hormone receptor gene expression in the oviduct and uterus during canine pregnancy. Nineteen pregnant bitches divided into four groups were ovariohysterectomized (OVH) at either day 8, 12, 21 or 60 of pregnancy, and five non-pregnant females underwent OVH 12days after the pre-ovulatory Luteinizant Hormone (LH) surge and served as controls. RT-qPCR for progesterone (PR), oestrogen (ER-a and ER-beta) and oxytocin (OTR) receptors was performed on the oviduct and uterine tissue. The mRNA PR expression in the uterus during early stages of pregnancy and the luteal phase was higher than at other times. The mRNA ER-beta expression in the oviduct during early pregnancy was less than in non-pregnant bitches. In the uterus, the mRNA ER-beta expression was higher in the initial stages of pregnancy. The ER-a expression was higher in the oviduct and uterus in advanced stages of pregnancy. The mRNA OTR expression in the oviduct was lower than in the uterus in control group. The expression of this receptor in oviduct and the uterus was higher in the final stages of pregnancy, when compared with other phases. These data suggested that the serum progesterone concentrations probably exert a direct control on the PR and ER (a and beta) expression and indirectly on OTR expression in the bitch oviduct and uterus. |
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Repositório Institucional da UNESP |
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Progesterone (PR), Oestrogen (ER-alpha and ER-beta) and Oxytocin (OTR) Gene Expression in the Oviduct and Uterus of Pregnant and Non-pregnant BitchesContents The aim was to assess hormone receptor gene expression in the oviduct and uterus during canine pregnancy. Nineteen pregnant bitches divided into four groups were ovariohysterectomized (OVH) at either day 8, 12, 21 or 60 of pregnancy, and five non-pregnant females underwent OVH 12days after the pre-ovulatory Luteinizant Hormone (LH) surge and served as controls. RT-qPCR for progesterone (PR), oestrogen (ER-a and ER-beta) and oxytocin (OTR) receptors was performed on the oviduct and uterine tissue. The mRNA PR expression in the uterus during early stages of pregnancy and the luteal phase was higher than at other times. The mRNA ER-beta expression in the oviduct during early pregnancy was less than in non-pregnant bitches. In the uterus, the mRNA ER-beta expression was higher in the initial stages of pregnancy. The ER-a expression was higher in the oviduct and uterus in advanced stages of pregnancy. The mRNA OTR expression in the oviduct was lower than in the uterus in control group. The expression of this receptor in oviduct and the uterus was higher in the final stages of pregnancy, when compared with other phases. These data suggested that the serum progesterone concentrations probably exert a direct control on the PR and ER (a and beta) expression and indirectly on OTR expression in the bitch oviduct and uterus.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Sch Vet Med & Anim Sci, São Paulo, BrazilUniv Estadual Paulista, IBB, Inst Biosci, São Paulo, BrazilUniv Estadual Paulista, Sch Vet Med & Anim Sci, São Paulo, BrazilUniv Estadual Paulista, IBB, Inst Biosci, São Paulo, BrazilFAPESP: 09/50718-0Wiley-BlackwellUniversidade Estadual Paulista (Unesp)Derussi, A. A. P. [UNESP]de Souza, R. W. A. [UNESP]Volpato, R. [UNESP]Guaitolini, C. R. F. [UNESP]Ackermann, C. L. [UNESP]Taffarel, M. O. [UNESP]Cardoso, G. S. [UNESP]Dal-Pai-Silva, M. [UNESP]Lopes, Maria Denise [UNESP]2014-05-20T15:31:54Z2014-05-20T15:31:54Z2012-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article197-199http://dx.doi.org/10.1111/rda.12015Reproduction In Domestic Animals. Hoboken: Wiley-blackwell, v. 47, p. 197-199, 2012.0936-6768http://hdl.handle.net/11449/4091910.1111/rda.12015WOS:0003128883000456666129914663018Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproduction in Domestic Animals1.4220,594info:eu-repo/semantics/openAccess2021-10-23T11:51:50Zoai:repositorio.unesp.br:11449/40919Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T11:51:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Progesterone (PR), Oestrogen (ER-alpha and ER-beta) and Oxytocin (OTR) Gene Expression in the Oviduct and Uterus of Pregnant and Non-pregnant Bitches |
title |
Progesterone (PR), Oestrogen (ER-alpha and ER-beta) and Oxytocin (OTR) Gene Expression in the Oviduct and Uterus of Pregnant and Non-pregnant Bitches |
spellingShingle |
Progesterone (PR), Oestrogen (ER-alpha and ER-beta) and Oxytocin (OTR) Gene Expression in the Oviduct and Uterus of Pregnant and Non-pregnant Bitches Derussi, A. A. P. [UNESP] |
title_short |
Progesterone (PR), Oestrogen (ER-alpha and ER-beta) and Oxytocin (OTR) Gene Expression in the Oviduct and Uterus of Pregnant and Non-pregnant Bitches |
title_full |
Progesterone (PR), Oestrogen (ER-alpha and ER-beta) and Oxytocin (OTR) Gene Expression in the Oviduct and Uterus of Pregnant and Non-pregnant Bitches |
title_fullStr |
Progesterone (PR), Oestrogen (ER-alpha and ER-beta) and Oxytocin (OTR) Gene Expression in the Oviduct and Uterus of Pregnant and Non-pregnant Bitches |
title_full_unstemmed |
Progesterone (PR), Oestrogen (ER-alpha and ER-beta) and Oxytocin (OTR) Gene Expression in the Oviduct and Uterus of Pregnant and Non-pregnant Bitches |
title_sort |
Progesterone (PR), Oestrogen (ER-alpha and ER-beta) and Oxytocin (OTR) Gene Expression in the Oviduct and Uterus of Pregnant and Non-pregnant Bitches |
author |
Derussi, A. A. P. [UNESP] |
author_facet |
Derussi, A. A. P. [UNESP] de Souza, R. W. A. [UNESP] Volpato, R. [UNESP] Guaitolini, C. R. F. [UNESP] Ackermann, C. L. [UNESP] Taffarel, M. O. [UNESP] Cardoso, G. S. [UNESP] Dal-Pai-Silva, M. [UNESP] Lopes, Maria Denise [UNESP] |
author_role |
author |
author2 |
de Souza, R. W. A. [UNESP] Volpato, R. [UNESP] Guaitolini, C. R. F. [UNESP] Ackermann, C. L. [UNESP] Taffarel, M. O. [UNESP] Cardoso, G. S. [UNESP] Dal-Pai-Silva, M. [UNESP] Lopes, Maria Denise [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Derussi, A. A. P. [UNESP] de Souza, R. W. A. [UNESP] Volpato, R. [UNESP] Guaitolini, C. R. F. [UNESP] Ackermann, C. L. [UNESP] Taffarel, M. O. [UNESP] Cardoso, G. S. [UNESP] Dal-Pai-Silva, M. [UNESP] Lopes, Maria Denise [UNESP] |
description |
Contents The aim was to assess hormone receptor gene expression in the oviduct and uterus during canine pregnancy. Nineteen pregnant bitches divided into four groups were ovariohysterectomized (OVH) at either day 8, 12, 21 or 60 of pregnancy, and five non-pregnant females underwent OVH 12days after the pre-ovulatory Luteinizant Hormone (LH) surge and served as controls. RT-qPCR for progesterone (PR), oestrogen (ER-a and ER-beta) and oxytocin (OTR) receptors was performed on the oviduct and uterine tissue. The mRNA PR expression in the uterus during early stages of pregnancy and the luteal phase was higher than at other times. The mRNA ER-beta expression in the oviduct during early pregnancy was less than in non-pregnant bitches. In the uterus, the mRNA ER-beta expression was higher in the initial stages of pregnancy. The ER-a expression was higher in the oviduct and uterus in advanced stages of pregnancy. The mRNA OTR expression in the oviduct was lower than in the uterus in control group. The expression of this receptor in oviduct and the uterus was higher in the final stages of pregnancy, when compared with other phases. These data suggested that the serum progesterone concentrations probably exert a direct control on the PR and ER (a and beta) expression and indirectly on OTR expression in the bitch oviduct and uterus. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-01 2014-05-20T15:31:54Z 2014-05-20T15:31:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/rda.12015 Reproduction In Domestic Animals. Hoboken: Wiley-blackwell, v. 47, p. 197-199, 2012. 0936-6768 http://hdl.handle.net/11449/40919 10.1111/rda.12015 WOS:000312888300045 6666129914663018 |
url |
http://dx.doi.org/10.1111/rda.12015 http://hdl.handle.net/11449/40919 |
identifier_str_mv |
Reproduction In Domestic Animals. Hoboken: Wiley-blackwell, v. 47, p. 197-199, 2012. 0936-6768 10.1111/rda.12015 WOS:000312888300045 6666129914663018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Reproduction in Domestic Animals 1.422 0,594 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
197-199 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell |
publisher.none.fl_str_mv |
Wiley-Blackwell |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046266382319616 |