Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device

Detalhes bibliográficos
Autor(a) principal: Rossini, Eduardo Luiz [UNESP]
Data de Publicação: 2018
Outros Autores: Milani, Maria Izabel [UNESP], Carrilho, Emanuel, Pezza, Leonardo [UNESP], Pezza, Helena Redigolo [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.aca.2017.10.018
http://hdl.handle.net/11449/179299
Resumo: In this paper, we describe a validated paper-based microfluidic analytical device for the simultaneous quantification of two important biomarkers of renal function in urine. This paper platform provides an inexpensive, simple, and easy to use colorimetric method for the quantification of creatinine (CRN) and uric acid (UA) in urine samples. The microfluidic paper-based analytical device (μPAD) consists of a main channel with three identical arms, each containing a circular testing zone and a circular uptake zone. Creatinine detection is based on the Jaffé reaction, in which CRN reacts with picrate to form an orange-red product. Uric acid quantification is based on the reduction of Fe3+ to Fe2+ by UA, which is detected in a colorimetric reaction using 1,10-phenanthroline. Under optimum conditions, obtained through chemometrics, the concentrations of the analytes showed good linear correlations with the effective intensities, and the method presented satisfactory repeatability. The limits of detection and the linear ranges, respectively, were 15.7 mg L−1 and 50–600 mg L−1 for CRN and 16.5 mg L−1 and 50–500 mg L−1 for UA. There were no statistically significant differences between the results obtained using the μPAD and a chromatographic comparative method (Student's t-test at 95% confidence level).
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spelling Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical deviceCreatinineDigital imagePaper-based microfluidic analytical device (μPAD)Renal functionUric acidUrineIn this paper, we describe a validated paper-based microfluidic analytical device for the simultaneous quantification of two important biomarkers of renal function in urine. This paper platform provides an inexpensive, simple, and easy to use colorimetric method for the quantification of creatinine (CRN) and uric acid (UA) in urine samples. The microfluidic paper-based analytical device (μPAD) consists of a main channel with three identical arms, each containing a circular testing zone and a circular uptake zone. Creatinine detection is based on the Jaffé reaction, in which CRN reacts with picrate to form an orange-red product. Uric acid quantification is based on the reduction of Fe3+ to Fe2+ by UA, which is detected in a colorimetric reaction using 1,10-phenanthroline. Under optimum conditions, obtained through chemometrics, the concentrations of the analytes showed good linear correlations with the effective intensities, and the method presented satisfactory repeatability. The limits of detection and the linear ranges, respectively, were 15.7 mg L−1 and 50–600 mg L−1 for CRN and 16.5 mg L−1 and 50–500 mg L−1 for UA. There were no statistically significant differences between the results obtained using the μPAD and a chromatographic comparative method (Student's t-test at 95% confidence level).Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Instituto de Química Universidade Estadual Paulista “Julio de Mesquita Filho”- UNESP, R. Prof. Francisco Degni 55, P.O. Box 355Instituto de Química de São Carlos Universidade de São Paulo, Av. Trabalhador São-carlense, 400Instituto de Química Universidade Estadual Paulista “Julio de Mesquita Filho”- UNESP, R. Prof. Francisco Degni 55, P.O. Box 355CNPq: 132646/2014-5CAPES: 2014/17749-7FAPESP: 2015/21733-1CNPq: 308788/2015-0Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Rossini, Eduardo Luiz [UNESP]Milani, Maria Izabel [UNESP]Carrilho, EmanuelPezza, Leonardo [UNESP]Pezza, Helena Redigolo [UNESP]2018-12-11T17:34:37Z2018-12-11T17:34:37Z2018-01-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16-23application/pdfhttp://dx.doi.org/10.1016/j.aca.2017.10.018Analytica Chimica Acta, v. 997, p. 16-23.1873-43240003-2670http://hdl.handle.net/11449/17929910.1016/j.aca.2017.10.0182-s2.0-850323331872-s2.0-85032333187.pdf5978908591853524Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnalytica Chimica Acta1,512info:eu-repo/semantics/openAccess2023-12-22T06:24:44Zoai:repositorio.unesp.br:11449/179299Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:02:07.406502Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device
title Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device
spellingShingle Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device
Rossini, Eduardo Luiz [UNESP]
Creatinine
Digital image
Paper-based microfluidic analytical device (μPAD)
Renal function
Uric acid
Urine
title_short Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device
title_full Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device
title_fullStr Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device
title_full_unstemmed Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device
title_sort Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device
author Rossini, Eduardo Luiz [UNESP]
author_facet Rossini, Eduardo Luiz [UNESP]
Milani, Maria Izabel [UNESP]
Carrilho, Emanuel
Pezza, Leonardo [UNESP]
Pezza, Helena Redigolo [UNESP]
author_role author
author2 Milani, Maria Izabel [UNESP]
Carrilho, Emanuel
Pezza, Leonardo [UNESP]
Pezza, Helena Redigolo [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Rossini, Eduardo Luiz [UNESP]
Milani, Maria Izabel [UNESP]
Carrilho, Emanuel
Pezza, Leonardo [UNESP]
Pezza, Helena Redigolo [UNESP]
dc.subject.por.fl_str_mv Creatinine
Digital image
Paper-based microfluidic analytical device (μPAD)
Renal function
Uric acid
Urine
topic Creatinine
Digital image
Paper-based microfluidic analytical device (μPAD)
Renal function
Uric acid
Urine
description In this paper, we describe a validated paper-based microfluidic analytical device for the simultaneous quantification of two important biomarkers of renal function in urine. This paper platform provides an inexpensive, simple, and easy to use colorimetric method for the quantification of creatinine (CRN) and uric acid (UA) in urine samples. The microfluidic paper-based analytical device (μPAD) consists of a main channel with three identical arms, each containing a circular testing zone and a circular uptake zone. Creatinine detection is based on the Jaffé reaction, in which CRN reacts with picrate to form an orange-red product. Uric acid quantification is based on the reduction of Fe3+ to Fe2+ by UA, which is detected in a colorimetric reaction using 1,10-phenanthroline. Under optimum conditions, obtained through chemometrics, the concentrations of the analytes showed good linear correlations with the effective intensities, and the method presented satisfactory repeatability. The limits of detection and the linear ranges, respectively, were 15.7 mg L−1 and 50–600 mg L−1 for CRN and 16.5 mg L−1 and 50–500 mg L−1 for UA. There were no statistically significant differences between the results obtained using the μPAD and a chromatographic comparative method (Student's t-test at 95% confidence level).
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:34:37Z
2018-12-11T17:34:37Z
2018-01-02
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.aca.2017.10.018
Analytica Chimica Acta, v. 997, p. 16-23.
1873-4324
0003-2670
http://hdl.handle.net/11449/179299
10.1016/j.aca.2017.10.018
2-s2.0-85032333187
2-s2.0-85032333187.pdf
5978908591853524
url http://dx.doi.org/10.1016/j.aca.2017.10.018
http://hdl.handle.net/11449/179299
identifier_str_mv Analytica Chimica Acta, v. 997, p. 16-23.
1873-4324
0003-2670
10.1016/j.aca.2017.10.018
2-s2.0-85032333187
2-s2.0-85032333187.pdf
5978908591853524
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Analytica Chimica Acta
1,512
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 16-23
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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