β-Glucan-induced cortisol levels improve the early immune response in matrinxã (Brycon amazonicus)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.fsi.2016.11.055 http://hdl.handle.net/11449/173879 |
Resumo: | This study investigated the role of endogenous cortisol on the innate immune response in matrinxã (Brycon amazonicus) fed with β-glucan, prior to and after stressor exposure and bacterial challenge. For this, we evaluated the serum cortisol and plasma glucose levels, the serum lysozyme levels, the hemolytic activity of the complement system, and the respiratory activity of leukocytes, as well as the number of circulating erythrocytes and leukocytes of fish fed during 15 days with diets containing β-glucan 0.1% (β-G) or β-glucan 0.1% + metyrapone 30 mg kg−1 fish (β-G + MTP). Dietary MTP was used to block cortisol production. After feeding, fish were air-exposed during 3 min, to endogenously increase the cortisol levels. Following that, they were challenged with intraperitoneal injection of Aeromonas hydrophila. Results were compared with a positive control group fed with a β-glucan-free diet. A negative control group, also fed with β-glucan-free diet but inoculated with PBS, was established to evaluate the effect of the handling during injection. Fish were sampled prior to the stressor exposure, 30 min after exposure, and 24 h post infection (hpi). Herein we observed that dietary β-G modulated the cortisol profile prior to and after the stressor, increasing the number and activity of leukocytes. Moreover, cortisol showed to be an efficient modulator of both humoral and cellular innate immune system by increasing lysozyme and complement activity, as well as neutrophil and monocyte populations. Our results suggest that β-glucan-induced cortisol increase is one important mechanism to improve the innate immune response in matrinxã. |
id |
UNSP_d7898c46cfe71b50a4564a2a9acbfe04 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/173879 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
β-Glucan-induced cortisol levels improve the early immune response in matrinxã (Brycon amazonicus)Acute infectionAcute stressCaptureEarly immune defenseHandlingThis study investigated the role of endogenous cortisol on the innate immune response in matrinxã (Brycon amazonicus) fed with β-glucan, prior to and after stressor exposure and bacterial challenge. For this, we evaluated the serum cortisol and plasma glucose levels, the serum lysozyme levels, the hemolytic activity of the complement system, and the respiratory activity of leukocytes, as well as the number of circulating erythrocytes and leukocytes of fish fed during 15 days with diets containing β-glucan 0.1% (β-G) or β-glucan 0.1% + metyrapone 30 mg kg−1 fish (β-G + MTP). Dietary MTP was used to block cortisol production. After feeding, fish were air-exposed during 3 min, to endogenously increase the cortisol levels. Following that, they were challenged with intraperitoneal injection of Aeromonas hydrophila. Results were compared with a positive control group fed with a β-glucan-free diet. A negative control group, also fed with β-glucan-free diet but inoculated with PBS, was established to evaluate the effect of the handling during injection. Fish were sampled prior to the stressor exposure, 30 min after exposure, and 24 h post infection (hpi). Herein we observed that dietary β-G modulated the cortisol profile prior to and after the stressor, increasing the number and activity of leukocytes. Moreover, cortisol showed to be an efficient modulator of both humoral and cellular innate immune system by increasing lysozyme and complement activity, as well as neutrophil and monocyte populations. Our results suggest that β-glucan-induced cortisol increase is one important mechanism to improve the innate immune response in matrinxã.Departamento de Morfologia e Fisiologia Animal Univ Estadual Paulista UNESP, Via de Acesso Prof. Paulo Donato CastelaneCentro de Aquicultura da UNESP (CAUNESP), Via de Acesso Prof. Paulo Donato CastelaneDepartamento de Morfologia e Fisiologia Animal Univ Estadual Paulista UNESP, Via de Acesso Prof. Paulo Donato CastelaneCentro de Aquicultura da UNESP (CAUNESP), Via de Acesso Prof. Paulo Donato CastelaneUniversidade Estadual Paulista (Unesp)Franco Montoya, Luz N. [UNESP]Martins, Talísia P. [UNESP]Gimbo, Rodrigo Y. [UNESP]Zanuzzo, Fábio S. [UNESP]Urbinati, Elisabeth C. [UNESP]2018-12-11T17:08:10Z2018-12-11T17:08:10Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article197-204application/pdfhttp://dx.doi.org/10.1016/j.fsi.2016.11.055Fish and Shellfish Immunology, v. 60, p. 197-204.1095-99471050-4648http://hdl.handle.net/11449/17387910.1016/j.fsi.2016.11.0552-s2.0-850021568722-s2.0-85002156872.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFish and Shellfish Immunology1,1261,126info:eu-repo/semantics/openAccess2024-04-09T15:36:53Zoai:repositorio.unesp.br:11449/173879Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:23:34.977592Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
β-Glucan-induced cortisol levels improve the early immune response in matrinxã (Brycon amazonicus) |
title |
β-Glucan-induced cortisol levels improve the early immune response in matrinxã (Brycon amazonicus) |
spellingShingle |
β-Glucan-induced cortisol levels improve the early immune response in matrinxã (Brycon amazonicus) Franco Montoya, Luz N. [UNESP] Acute infection Acute stress Capture Early immune defense Handling |
title_short |
β-Glucan-induced cortisol levels improve the early immune response in matrinxã (Brycon amazonicus) |
title_full |
β-Glucan-induced cortisol levels improve the early immune response in matrinxã (Brycon amazonicus) |
title_fullStr |
β-Glucan-induced cortisol levels improve the early immune response in matrinxã (Brycon amazonicus) |
title_full_unstemmed |
β-Glucan-induced cortisol levels improve the early immune response in matrinxã (Brycon amazonicus) |
title_sort |
β-Glucan-induced cortisol levels improve the early immune response in matrinxã (Brycon amazonicus) |
author |
Franco Montoya, Luz N. [UNESP] |
author_facet |
Franco Montoya, Luz N. [UNESP] Martins, Talísia P. [UNESP] Gimbo, Rodrigo Y. [UNESP] Zanuzzo, Fábio S. [UNESP] Urbinati, Elisabeth C. [UNESP] |
author_role |
author |
author2 |
Martins, Talísia P. [UNESP] Gimbo, Rodrigo Y. [UNESP] Zanuzzo, Fábio S. [UNESP] Urbinati, Elisabeth C. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Franco Montoya, Luz N. [UNESP] Martins, Talísia P. [UNESP] Gimbo, Rodrigo Y. [UNESP] Zanuzzo, Fábio S. [UNESP] Urbinati, Elisabeth C. [UNESP] |
dc.subject.por.fl_str_mv |
Acute infection Acute stress Capture Early immune defense Handling |
topic |
Acute infection Acute stress Capture Early immune defense Handling |
description |
This study investigated the role of endogenous cortisol on the innate immune response in matrinxã (Brycon amazonicus) fed with β-glucan, prior to and after stressor exposure and bacterial challenge. For this, we evaluated the serum cortisol and plasma glucose levels, the serum lysozyme levels, the hemolytic activity of the complement system, and the respiratory activity of leukocytes, as well as the number of circulating erythrocytes and leukocytes of fish fed during 15 days with diets containing β-glucan 0.1% (β-G) or β-glucan 0.1% + metyrapone 30 mg kg−1 fish (β-G + MTP). Dietary MTP was used to block cortisol production. After feeding, fish were air-exposed during 3 min, to endogenously increase the cortisol levels. Following that, they were challenged with intraperitoneal injection of Aeromonas hydrophila. Results were compared with a positive control group fed with a β-glucan-free diet. A negative control group, also fed with β-glucan-free diet but inoculated with PBS, was established to evaluate the effect of the handling during injection. Fish were sampled prior to the stressor exposure, 30 min after exposure, and 24 h post infection (hpi). Herein we observed that dietary β-G modulated the cortisol profile prior to and after the stressor, increasing the number and activity of leukocytes. Moreover, cortisol showed to be an efficient modulator of both humoral and cellular innate immune system by increasing lysozyme and complement activity, as well as neutrophil and monocyte populations. Our results suggest that β-glucan-induced cortisol increase is one important mechanism to improve the innate immune response in matrinxã. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T17:08:10Z 2018-12-11T17:08:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.fsi.2016.11.055 Fish and Shellfish Immunology, v. 60, p. 197-204. 1095-9947 1050-4648 http://hdl.handle.net/11449/173879 10.1016/j.fsi.2016.11.055 2-s2.0-85002156872 2-s2.0-85002156872.pdf |
url |
http://dx.doi.org/10.1016/j.fsi.2016.11.055 http://hdl.handle.net/11449/173879 |
identifier_str_mv |
Fish and Shellfish Immunology, v. 60, p. 197-204. 1095-9947 1050-4648 10.1016/j.fsi.2016.11.055 2-s2.0-85002156872 2-s2.0-85002156872.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fish and Shellfish Immunology 1,126 1,126 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
197-204 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129196328747008 |