Efeito mutagênico do edetato de cobre ([Cu(EDTA)]-2) livre e nanoencapsulado em camundongos e peixes

• Autor(a) principal: Jacacobowski, Ana Cristina
• Data de Publicação: 2013
• Outros Autores: Zobiole, Natalia Novak, Padilha, Pedro de Magalhães [UNESP], Moreno, Susana Elisa, Arruda, Eduardo José de
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.5132/eec.2013.01.002; http://hdl.handle.net/11449/141227
• topic: Copper edetate; Fish; Micronucleus test; Mouse; Mutagenicity; Nanocapsules; Camundongos; Edetato de cobre; Mutagenicidade; Nanocápsulas; Peixes; Teste do micronúcleo

Recent studies show that copper complexes with ethylenediaminetetraacetic acid ([Cu(EDTA)]-2), is lethal for Aedes aegypti larvae. This compound may have a controlled release when nanocapsules complex in gum/gelatin/chitosan, which may limit their toxicity to the environment. However, the genotoxicity of [Cu(EDTA)]-2 is not fully elucidated. To assess the mutagenic potential of [Cu(EDTA)]-2, we analyzed the frequency of micronuclei in peripheral blood of mice and fish subjected to free and nanoencapsulated [Cu(EDTA)]-2. The carcass of the fish was assessed by mass spectrometry to measure tissue copper. In mice, the frequency of micronuclei in animals exposed to free [Cu(EDTA)]-2 was significantly higher in the animals submitted to the nanoencapsulated form. In fish, only the free [Cu(EDTA)]-2 was able to induce increased frequency of micronuclei. Mass spectrometric analysis showed copper accumulation in the fish’s liver studied, with significant difference in the control. In the gills of fish were not observed differences in copper concentration between the groups and the copper content was significantly lower than that found in the liver. The results suggest that free [Cu(EDTA)]-2 can induce mutagenic effects in mammals, but the nanoencapsulated seems to give more security to the product due to its slower release.