Inhibition of human neutrophil apoptosis by Paracoccidioides brasiliensis: Role of interleukin-8

• Autor(a) principal: Acorci, M. J. [UNESP]
• Data de Publicação: 2009
• Outros Autores: Dias-Melicio, L. A. [UNESP], Golim, M. A. [UNESP], Bordon-Graciani, A. P. [UNESP], Peraçoli, M. T S [UNESP], Soares, A. M V C [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1111/j.1365-3083.2008.02199.x; http://hdl.handle.net/11449/70928
• topic: interleukin 8; interleukin 8 antibody; adult; apoptosis; cell culture; cell level; cell survival; controlled study; cytokine production; cytokine release; enzyme linked immunosorbent assay; flow cytometry; human; human cell; neutrophil; normal human; Paracoccidioides brasiliensis; phagocyte; priority journal; yeast cell; Adult; Antibodies, Monoclonal; Apoptosis; Humans; Interleukin-8; Middle Aged; Neutrophils; Paracoccidioides; Phagocytosis

Paracoccidioidomycosis (PCM) is a systemic mycosis caused by Paracoccidiodes brasiliensis that presents a wide spectrum of clinical manifestations. Because of the great number of neutrophils polymorphonuclear neutrophils (PMN) found in the P. brasiliensis granuloma, studies have been done to evaluate the role of these cells during the development of the infection. This fungus is found intracellularly in PMN and monocytes/macrophages, suggesting that it is capable of evading damage and surviving inside these cells. Thus, in the present study, we investigated whether P. brasiliensis can prolong the lifetime of PMN, and if this process would be related with IL-8 levels. PMN apoptosis and intracellular levels of IL-8 were analysed by flow cytometry and culture supernatants IL-8 levels were evaluated by enzyme-linked immunosorbent assay. We found that coincubation with P. brasiliensis yeast cells results in an inhibition of PMN apoptosis, which was associated with increase in IL-8 production by these cells. Cocultures treatment with monoclonal antibody anti-IL-8 reversed the inhibitory effect of P. brasiliensis on PMN apoptosis, besides to increase spontaneous apoptosis of these cells. These data show that, in contrast to other microbial pathogens that drive phagocytes into apoptosis to escape killing, P. brasiliensis can extend the lifetime of normal human PMN by inducing autocrine IL-8 production. © 2008 The Authors.