Growth mechanisms of Ca- and P-rich MAO films in Ti-15Zr-xMo alloys for osseointegrative implants
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.surfcoat.2018.02.099 http://hdl.handle.net/11449/170791 |
Resumo: | In this study, a micro-arc oxidation treatment was applied to Ti-15Zr-xMo (x = 0, 5, 10 and 15 wt%) alloys to produce porous oxide layers enriched with bioactive ions (calcium and phosphorus) for use as osseointegrative implants. Biocompatibility studies, namely metabolic activity, mineralization and differentiation studies were conducted with human osteoblastic cell line SAOS-2. A typical porous coating was obtained in all samples, with similar morphologies and thicknesses, which were found to be dependent on the maximum applied voltage. Calcium and phosphorus ions were incorporated into the films, as indicated by EDX analysis. Chemical analyses indicated that the films were composed preferentially of Ti and Zr oxides. XRD patterns revealed mostly substrate Ti phases. However, cross-sectional TEM imaging and automated phase and orientation mapping showed distinct amorphous and nanocrystalline regions within the films, with a higher fraction of Ca atoms incorporated in the outer layer. After immersion in Hanks’ Balanced Salt Solution (HBSS) for seven days, small amounts of calcium phosphate precipitates were observed at the surface of all samples which were confirmed by ICP-AES measurements, indicating that the MAO treatment possibly introduced a considerable bioactive response in the samples. Biological results indicate that Ti-15Zr-15Mo MAO-treated surfaces are biocompatible and induce a higher osteoblasts viability and mineralization. The combination of porous structure and bioactive composition of the oxide layers can be suitable for use as advanced biomedical implants with osseointegration ability. |
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Growth mechanisms of Ca- and P-rich MAO films in Ti-15Zr-xMo alloys for osseointegrative implantsBioactivityCrystalline structureMicro-arc oxidationMineralizationTi-Zr-Mo alloyIn this study, a micro-arc oxidation treatment was applied to Ti-15Zr-xMo (x = 0, 5, 10 and 15 wt%) alloys to produce porous oxide layers enriched with bioactive ions (calcium and phosphorus) for use as osseointegrative implants. Biocompatibility studies, namely metabolic activity, mineralization and differentiation studies were conducted with human osteoblastic cell line SAOS-2. A typical porous coating was obtained in all samples, with similar morphologies and thicknesses, which were found to be dependent on the maximum applied voltage. Calcium and phosphorus ions were incorporated into the films, as indicated by EDX analysis. Chemical analyses indicated that the films were composed preferentially of Ti and Zr oxides. XRD patterns revealed mostly substrate Ti phases. However, cross-sectional TEM imaging and automated phase and orientation mapping showed distinct amorphous and nanocrystalline regions within the films, with a higher fraction of Ca atoms incorporated in the outer layer. After immersion in Hanks’ Balanced Salt Solution (HBSS) for seven days, small amounts of calcium phosphate precipitates were observed at the surface of all samples which were confirmed by ICP-AES measurements, indicating that the MAO treatment possibly introduced a considerable bioactive response in the samples. Biological results indicate that Ti-15Zr-15Mo MAO-treated surfaces are biocompatible and induce a higher osteoblasts viability and mineralization. The combination of porous structure and bioactive composition of the oxide layers can be suitable for use as advanced biomedical implants with osseointegration ability.Japan Agency for Medical Research and DevelopmentFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)IBTN/BR – Brazilian Branch Institute of Biomaterials Tribocorrosion and NanomedicineIFSP – Federal Institute of Education Science and TechnologyUNESP – Univ Estadual Paulista Laboratório de Anelasticidade e BiomateriaisPostgraduate Program in Biotechnology National Institute of Metrology Quality and TechnologyUNIGRANRIO - University of Grande Rio Post-Graduate Program on Translational BiomedicineINMETRO - National Institute of Metrology Quality and Technology Metrology Materials DivisionCBPF – Centro Brasileiro de Pesquisas FísicasUFSCar – Federal University of São Carlos Department of Materials EngineeringTMDU - Tokyo Medical and Dental University Graduate School of Medical and Dental SciencesTMDU - Tokyo Medical and Dental University Institute of Biomaterials and BioengineeringThe University of Tokyo Graduate School of EngineeringUNESP – Univ Estadual Paulista Laboratório de Anelasticidade e BiomateriaisFAPESP: 00851-6/2015CNPq: 207417/2015-6CAPES: 99999.008666/2014-08Tribocorrosion and NanomedicineScience and TechnologyUniversidade Estadual Paulista (Unesp)Quality and TechnologyPost-Graduate Program on Translational BiomedicineMetrology Materials DivisionCBPF – Centro Brasileiro de Pesquisas FísicasUniversidade Federal de São Carlos (UFSCar)Graduate School of Medical and Dental SciencesInstitute of Biomaterials and BioengineeringGraduate School of EngineeringCorrea, D. R.N.Rocha, L. A. [UNESP]Ribeiro, A. R.Gemini-Piperni, S.Archanjo, B. S.Achete, C. A.Werckmann, J.Afonso, C. R.M.Shimabukuro, M.Doi, H.Tsutsumi, Y.Hanawa, T.2018-12-11T16:52:26Z2018-12-11T16:52:26Z2018-06-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article373-382application/pdfhttp://dx.doi.org/10.1016/j.surfcoat.2018.02.099Surface and Coatings Technology, v. 344, p. 373-382.0257-8972http://hdl.handle.net/11449/17079110.1016/j.surfcoat.2018.02.0992-s2.0-850441373842-s2.0-85044137384.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSurface and Coatings Technology0,928info:eu-repo/semantics/openAccess2024-01-29T06:29:07Zoai:repositorio.unesp.br:11449/170791Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:13:43.735423Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Growth mechanisms of Ca- and P-rich MAO films in Ti-15Zr-xMo alloys for osseointegrative implants |
title |
Growth mechanisms of Ca- and P-rich MAO films in Ti-15Zr-xMo alloys for osseointegrative implants |
spellingShingle |
Growth mechanisms of Ca- and P-rich MAO films in Ti-15Zr-xMo alloys for osseointegrative implants Correa, D. R.N. Bioactivity Crystalline structure Micro-arc oxidation Mineralization Ti-Zr-Mo alloy |
title_short |
Growth mechanisms of Ca- and P-rich MAO films in Ti-15Zr-xMo alloys for osseointegrative implants |
title_full |
Growth mechanisms of Ca- and P-rich MAO films in Ti-15Zr-xMo alloys for osseointegrative implants |
title_fullStr |
Growth mechanisms of Ca- and P-rich MAO films in Ti-15Zr-xMo alloys for osseointegrative implants |
title_full_unstemmed |
Growth mechanisms of Ca- and P-rich MAO films in Ti-15Zr-xMo alloys for osseointegrative implants |
title_sort |
Growth mechanisms of Ca- and P-rich MAO films in Ti-15Zr-xMo alloys for osseointegrative implants |
author |
Correa, D. R.N. |
author_facet |
Correa, D. R.N. Rocha, L. A. [UNESP] Ribeiro, A. R. Gemini-Piperni, S. Archanjo, B. S. Achete, C. A. Werckmann, J. Afonso, C. R.M. Shimabukuro, M. Doi, H. Tsutsumi, Y. Hanawa, T. |
author_role |
author |
author2 |
Rocha, L. A. [UNESP] Ribeiro, A. R. Gemini-Piperni, S. Archanjo, B. S. Achete, C. A. Werckmann, J. Afonso, C. R.M. Shimabukuro, M. Doi, H. Tsutsumi, Y. Hanawa, T. |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Tribocorrosion and Nanomedicine Science and Technology Universidade Estadual Paulista (Unesp) Quality and Technology Post-Graduate Program on Translational Biomedicine Metrology Materials Division CBPF – Centro Brasileiro de Pesquisas Físicas Universidade Federal de São Carlos (UFSCar) Graduate School of Medical and Dental Sciences Institute of Biomaterials and Bioengineering Graduate School of Engineering |
dc.contributor.author.fl_str_mv |
Correa, D. R.N. Rocha, L. A. [UNESP] Ribeiro, A. R. Gemini-Piperni, S. Archanjo, B. S. Achete, C. A. Werckmann, J. Afonso, C. R.M. Shimabukuro, M. Doi, H. Tsutsumi, Y. Hanawa, T. |
dc.subject.por.fl_str_mv |
Bioactivity Crystalline structure Micro-arc oxidation Mineralization Ti-Zr-Mo alloy |
topic |
Bioactivity Crystalline structure Micro-arc oxidation Mineralization Ti-Zr-Mo alloy |
description |
In this study, a micro-arc oxidation treatment was applied to Ti-15Zr-xMo (x = 0, 5, 10 and 15 wt%) alloys to produce porous oxide layers enriched with bioactive ions (calcium and phosphorus) for use as osseointegrative implants. Biocompatibility studies, namely metabolic activity, mineralization and differentiation studies were conducted with human osteoblastic cell line SAOS-2. A typical porous coating was obtained in all samples, with similar morphologies and thicknesses, which were found to be dependent on the maximum applied voltage. Calcium and phosphorus ions were incorporated into the films, as indicated by EDX analysis. Chemical analyses indicated that the films were composed preferentially of Ti and Zr oxides. XRD patterns revealed mostly substrate Ti phases. However, cross-sectional TEM imaging and automated phase and orientation mapping showed distinct amorphous and nanocrystalline regions within the films, with a higher fraction of Ca atoms incorporated in the outer layer. After immersion in Hanks’ Balanced Salt Solution (HBSS) for seven days, small amounts of calcium phosphate precipitates were observed at the surface of all samples which were confirmed by ICP-AES measurements, indicating that the MAO treatment possibly introduced a considerable bioactive response in the samples. Biological results indicate that Ti-15Zr-15Mo MAO-treated surfaces are biocompatible and induce a higher osteoblasts viability and mineralization. The combination of porous structure and bioactive composition of the oxide layers can be suitable for use as advanced biomedical implants with osseointegration ability. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:52:26Z 2018-12-11T16:52:26Z 2018-06-25 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.surfcoat.2018.02.099 Surface and Coatings Technology, v. 344, p. 373-382. 0257-8972 http://hdl.handle.net/11449/170791 10.1016/j.surfcoat.2018.02.099 2-s2.0-85044137384 2-s2.0-85044137384.pdf |
url |
http://dx.doi.org/10.1016/j.surfcoat.2018.02.099 http://hdl.handle.net/11449/170791 |
identifier_str_mv |
Surface and Coatings Technology, v. 344, p. 373-382. 0257-8972 10.1016/j.surfcoat.2018.02.099 2-s2.0-85044137384 2-s2.0-85044137384.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Surface and Coatings Technology 0,928 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
373-382 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129597414309888 |