Caracterização do transcriptoma do músculo estriado esquelético do pacu (Piaractus mesopotamicus)

• Autor(a) principal: Mareco, Edson Assunção [UNESP]
• Data de Publicação: 2015
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/144035; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/24-08-2016/000869173.pdf
• topic: Proteínas; Ubiquitina; Pacu (Peixe); Expressão gênica; Sistema musculoesquelético; Jejum; Musculoskeletal system; Fishes

Pacu (Piaractus mesopotamicus) has excellent husbandry features like rapid growth. This parameter is directly related to the increase of skeletal muscle. Researches involving the characterization of muscle genes have been performed using molecular technologies, in species of fish such as zebrafish model that has sequenced the genome. In pacu, a species that does not have the sequenced genome, this lack of genetic information makes it difficult to conduct research related to the identification of signaling pathways that regulate development, growth and maintenance of muscle phenotype. Moreover, the lack of information promotes a limitation for the development of breeding programs for this species. The main objectives of this study were: 1) to increase the available genetic resources for the species, 2) compare the transcriptomes of red and white muscle types, exploring the signaling pathways that control the types of muscle fibers 3) to investigate the expression of deubiquitins belonging to the family of ubiquitin-specific proteases system (USP) in muscle in response to fasting and feedback. With the sequencing, it was obtained approximately 0.6Tb readings from red skeletal muscle and white libraries. Approximately 665 million readings were used to perform the assembly, resulting in 504 065 contigs with an average length of N50 = 1,334bp and 2,772bp; 47% of the transcriptome was noted successfully, which can be identified about 15,000 genes considered as unique and approximately 8000 sequences coding complete region. Among the identified genes, we could observe 319 metabolic pathways genes and 380 microsatellite. Nine hundred fifty six and 604 genes were differentially expressed between red and white muscle, respectively. We identified 442 pairs of paralogs genes resulting from genomic duplication at the base source of teleost fish. It was possible to identify differential expression of paralogous genes include the types of fibers...