Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1677-55382012000100003 http://hdl.handle.net/11449/231290 |
Resumo: | Objective: The aim of the study was to develop a new durable animal model (using rabbits) for anatomical-functional evaluation of urethral sphincter deficiency. Materials and Methods: A total of 40 new Zealand male rabbits, weighting 2.500 kg to 3.100 kg, were evaluated to develop an incontinent animal model. Thirty-two animals underwent urethrolysis and 8 animals received sham operation. Before and at 2, 4, 8 and 12 weeks after urethrolysis or sham operation, it was performed cystometry and leak point pressure (LPP) evaluation with different bladder distension volumes (10, 20, 30 mL). In each time point, 10 animals (8 from the study group and 2 from the sham group) were sacrificed to harvest the bladder and urethra. The samples were evaluated by H & E and Masson's Trichrome to determine urethral morphology and collagen/smooth muscle density. Results: Twelve weeks after urethrolysis, it was observed a significant decrease in LPP regardless the bladder volume (from 33.7 ± 6.6 to 12.8 ± 2.2 cmH2O). The histological analysis evidenced a decrease of 22% in smooth muscle density with a proportional increase in the collagen, vessels and elastin density (p < 0.01). Conclusions: Transabdominal urethrolysis develops urethral sphincter insufficiency in rabbits, with significant decrease in LPP associated with decrease of smooth muscle fibers and increase of collagen density. This animal model can be used to test autologous cell therapy for stress urinary incontinence treatment. |
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Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluationAnimal modelStem cellsTissue engineeringTransabdominal urethrolysisUrethral sphincter deficiencyUrinary incontinenceObjective: The aim of the study was to develop a new durable animal model (using rabbits) for anatomical-functional evaluation of urethral sphincter deficiency. Materials and Methods: A total of 40 new Zealand male rabbits, weighting 2.500 kg to 3.100 kg, were evaluated to develop an incontinent animal model. Thirty-two animals underwent urethrolysis and 8 animals received sham operation. Before and at 2, 4, 8 and 12 weeks after urethrolysis or sham operation, it was performed cystometry and leak point pressure (LPP) evaluation with different bladder distension volumes (10, 20, 30 mL). In each time point, 10 animals (8 from the study group and 2 from the sham group) were sacrificed to harvest the bladder and urethra. The samples were evaluated by H & E and Masson's Trichrome to determine urethral morphology and collagen/smooth muscle density. Results: Twelve weeks after urethrolysis, it was observed a significant decrease in LPP regardless the bladder volume (from 33.7 ± 6.6 to 12.8 ± 2.2 cmH2O). The histological analysis evidenced a decrease of 22% in smooth muscle density with a proportional increase in the collagen, vessels and elastin density (p < 0.01). Conclusions: Transabdominal urethrolysis develops urethral sphincter insufficiency in rabbits, with significant decrease in LPP associated with decrease of smooth muscle fibers and increase of collagen density. This animal model can be used to test autologous cell therapy for stress urinary incontinence treatment.Department of Urology School of Medicine Federal University of Sao Paulo, Sao PauloLaboratory of Medical Investigation - Department of Urology School of Medicine State University of Sao Paulo, Sao PauloUniversidade de São Paulo (USP)Skaff, M.Pinto, E. R.S.Leite, K. R.M.Almeida, F. G.2022-04-29T08:44:30Z2022-04-29T08:44:30Z2012-08-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17-24http://dx.doi.org/10.1590/S1677-55382012000100003International Braz J Urol, v. 38, n. 1, p. 17-24, 2012.1677-55381677-6119http://hdl.handle.net/11449/23129010.1590/S1677-553820120001000032-s2.0-84865358380Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Braz J Urolinfo:eu-repo/semantics/openAccess2024-09-03T14:29:54Zoai:repositorio.unesp.br:11449/231290Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T14:29:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation |
title |
Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation |
spellingShingle |
Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation Skaff, M. Animal model Stem cells Tissue engineering Transabdominal urethrolysis Urethral sphincter deficiency Urinary incontinence |
title_short |
Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation |
title_full |
Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation |
title_fullStr |
Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation |
title_full_unstemmed |
Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation |
title_sort |
Development of a rabbit's urethral sphincter deficiency animal model for anatomical-functional evaluation |
author |
Skaff, M. |
author_facet |
Skaff, M. Pinto, E. R.S. Leite, K. R.M. Almeida, F. G. |
author_role |
author |
author2 |
Pinto, E. R.S. Leite, K. R.M. Almeida, F. G. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Skaff, M. Pinto, E. R.S. Leite, K. R.M. Almeida, F. G. |
dc.subject.por.fl_str_mv |
Animal model Stem cells Tissue engineering Transabdominal urethrolysis Urethral sphincter deficiency Urinary incontinence |
topic |
Animal model Stem cells Tissue engineering Transabdominal urethrolysis Urethral sphincter deficiency Urinary incontinence |
description |
Objective: The aim of the study was to develop a new durable animal model (using rabbits) for anatomical-functional evaluation of urethral sphincter deficiency. Materials and Methods: A total of 40 new Zealand male rabbits, weighting 2.500 kg to 3.100 kg, were evaluated to develop an incontinent animal model. Thirty-two animals underwent urethrolysis and 8 animals received sham operation. Before and at 2, 4, 8 and 12 weeks after urethrolysis or sham operation, it was performed cystometry and leak point pressure (LPP) evaluation with different bladder distension volumes (10, 20, 30 mL). In each time point, 10 animals (8 from the study group and 2 from the sham group) were sacrificed to harvest the bladder and urethra. The samples were evaluated by H & E and Masson's Trichrome to determine urethral morphology and collagen/smooth muscle density. Results: Twelve weeks after urethrolysis, it was observed a significant decrease in LPP regardless the bladder volume (from 33.7 ± 6.6 to 12.8 ± 2.2 cmH2O). The histological analysis evidenced a decrease of 22% in smooth muscle density with a proportional increase in the collagen, vessels and elastin density (p < 0.01). Conclusions: Transabdominal urethrolysis develops urethral sphincter insufficiency in rabbits, with significant decrease in LPP associated with decrease of smooth muscle fibers and increase of collagen density. This animal model can be used to test autologous cell therapy for stress urinary incontinence treatment. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-08-30 2022-04-29T08:44:30Z 2022-04-29T08:44:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1677-55382012000100003 International Braz J Urol, v. 38, n. 1, p. 17-24, 2012. 1677-5538 1677-6119 http://hdl.handle.net/11449/231290 10.1590/S1677-55382012000100003 2-s2.0-84865358380 |
url |
http://dx.doi.org/10.1590/S1677-55382012000100003 http://hdl.handle.net/11449/231290 |
identifier_str_mv |
International Braz J Urol, v. 38, n. 1, p. 17-24, 2012. 1677-5538 1677-6119 10.1590/S1677-55382012000100003 2-s2.0-84865358380 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Braz J Urol |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
17-24 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021356384813056 |