PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1415-47572007000500020 http://hdl.handle.net/11449/30499 |
Resumo: | In silico expression profiles, of the discovered 3,103 citrus ESTs putatively encoding for PR protein families (PR-1 to PR-17), were evaluated using the Brazil citrus genome EST CitEST/database. Hierarchical clustering was displayed to identify similarities in expression patterns among citrus PR-like gene families (PRlgf) in 33 selected cDNA libraries. In this way, PRlgf preferentially expressed by organ and citrus species, and library conditions were highlighted. Changes in expression profiles of clusters for each of the 17 PRlgf expressed in organs infected by pathogens or drought-stressed citrus species were displayed for relative suppression or induction gene expression in relation to the counterpart control. Overall, few PRlgf showed expression 2-fold higher in pathogen-infected than in uninfected organs, even though the differential expression profiles displayed have been quite diverse among studied species and organs. Furthermore, an insight into some contigs from four PRlgf pointed out putative members of multigene families. They appear to be evolutionarily conserved within citrus species and/or organ- or stress-specifically expressed. Our results represent a starting point regarding the extent of expression pattern differences underlying PRlgf expression and reveal genes that may prove to be useful in studies regarding biotechnological approaches or citrus resistance markers. |
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PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approachcitrusfunctional genomeestsgene expression profilespathogenesis-related proteinsdefense genesIn silico expression profiles, of the discovered 3,103 citrus ESTs putatively encoding for PR protein families (PR-1 to PR-17), were evaluated using the Brazil citrus genome EST CitEST/database. Hierarchical clustering was displayed to identify similarities in expression patterns among citrus PR-like gene families (PRlgf) in 33 selected cDNA libraries. In this way, PRlgf preferentially expressed by organ and citrus species, and library conditions were highlighted. Changes in expression profiles of clusters for each of the 17 PRlgf expressed in organs infected by pathogens or drought-stressed citrus species were displayed for relative suppression or induction gene expression in relation to the counterpart control. Overall, few PRlgf showed expression 2-fold higher in pathogen-infected than in uninfected organs, even though the differential expression profiles displayed have been quite diverse among studied species and organs. Furthermore, an insight into some contigs from four PRlgf pointed out putative members of multigene families. They appear to be evolutionarily conserved within citrus species and/or organ- or stress-specifically expressed. Our results represent a starting point regarding the extent of expression pattern differences underlying PRlgf expression and reveal genes that may prove to be useful in studies regarding biotechnological approaches or citrus resistance markers.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal de Lavras (UFLA) Departamento de BiologiaUniversidade Estadual Paulista Departamento de Produção VegetalUniversidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz Departamento de Fitopatologia e GenéticaInstituto Agronômico de Campinas Centro APTA Citros Sylvio MoreiraUniversidade Estadual de Campinas Departamento de Microbiologia e ImunologiaUniversidade Estadual Paulista Departamento de Produção VegetalSociedade Brasileira de GenéticaUniversidade Federal de Lavras (UFLA)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Agência Paulista de Tecnologia dos Agronegócios (APTA)Universidade Estadual de Campinas (UNICAMP)Campos, Magnólia A.Rosa, Daniel D. [UNESP]Teixeira, Juliana Érika C.Targon, Maria Luisa P.N.Souza, Alessandra A.Paiva, Luciano V.Stach-Machado, Dagmar R.Machado, Marcos A.2014-05-20T15:17:30Z2014-05-20T15:17:30Z2007-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article917-930application/pdfhttp://dx.doi.org/10.1590/S1415-47572007000500020Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 3, p. 917-930, 2007.1415-4757http://hdl.handle.net/11449/3049910.1590/S1415-47572007000500020S1415-47572007000500020S1415-47572007000500020.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-12-31T06:19:41Zoai:repositorio.unesp.br:11449/30499Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:47:02.357136Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach |
title |
PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach |
spellingShingle |
PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach Campos, Magnólia A. citrus functional genome ests gene expression profiles pathogenesis-related proteins defense genes |
title_short |
PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach |
title_full |
PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach |
title_fullStr |
PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach |
title_full_unstemmed |
PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach |
title_sort |
PR gene families of citrus: their organ specific-biotic and abiotic inducible expression profiles based on ESTs approach |
author |
Campos, Magnólia A. |
author_facet |
Campos, Magnólia A. Rosa, Daniel D. [UNESP] Teixeira, Juliana Érika C. Targon, Maria Luisa P.N. Souza, Alessandra A. Paiva, Luciano V. Stach-Machado, Dagmar R. Machado, Marcos A. |
author_role |
author |
author2 |
Rosa, Daniel D. [UNESP] Teixeira, Juliana Érika C. Targon, Maria Luisa P.N. Souza, Alessandra A. Paiva, Luciano V. Stach-Machado, Dagmar R. Machado, Marcos A. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Lavras (UFLA) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Agência Paulista de Tecnologia dos Agronegócios (APTA) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Campos, Magnólia A. Rosa, Daniel D. [UNESP] Teixeira, Juliana Érika C. Targon, Maria Luisa P.N. Souza, Alessandra A. Paiva, Luciano V. Stach-Machado, Dagmar R. Machado, Marcos A. |
dc.subject.por.fl_str_mv |
citrus functional genome ests gene expression profiles pathogenesis-related proteins defense genes |
topic |
citrus functional genome ests gene expression profiles pathogenesis-related proteins defense genes |
description |
In silico expression profiles, of the discovered 3,103 citrus ESTs putatively encoding for PR protein families (PR-1 to PR-17), were evaluated using the Brazil citrus genome EST CitEST/database. Hierarchical clustering was displayed to identify similarities in expression patterns among citrus PR-like gene families (PRlgf) in 33 selected cDNA libraries. In this way, PRlgf preferentially expressed by organ and citrus species, and library conditions were highlighted. Changes in expression profiles of clusters for each of the 17 PRlgf expressed in organs infected by pathogens or drought-stressed citrus species were displayed for relative suppression or induction gene expression in relation to the counterpart control. Overall, few PRlgf showed expression 2-fold higher in pathogen-infected than in uninfected organs, even though the differential expression profiles displayed have been quite diverse among studied species and organs. Furthermore, an insight into some contigs from four PRlgf pointed out putative members of multigene families. They appear to be evolutionarily conserved within citrus species and/or organ- or stress-specifically expressed. Our results represent a starting point regarding the extent of expression pattern differences underlying PRlgf expression and reveal genes that may prove to be useful in studies regarding biotechnological approaches or citrus resistance markers. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-01-01 2014-05-20T15:17:30Z 2014-05-20T15:17:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1415-47572007000500020 Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 3, p. 917-930, 2007. 1415-4757 http://hdl.handle.net/11449/30499 10.1590/S1415-47572007000500020 S1415-47572007000500020 S1415-47572007000500020.pdf |
url |
http://dx.doi.org/10.1590/S1415-47572007000500020 http://hdl.handle.net/11449/30499 |
identifier_str_mv |
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 3, p. 917-930, 2007. 1415-4757 10.1590/S1415-47572007000500020 S1415-47572007000500020 S1415-47572007000500020.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology 1.493 0,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
917-930 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129357793722368 |