Ultrastructural Study of the Denervated Diaphragm in Rats

Detalhes bibliográficos
Autor(a) principal: Torrejais, M. M.
Data de Publicação: 2013
Outros Autores: Soares, J. C. [UNESP], Matheus, S. M. M. [UNESP], Mello, J. M., Francia-Farje, L. A. D., Jeronimo, L. C., Vicente, E. J. D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.4067/S0717-95022013000300025
http://hdl.handle.net/11449/111487
Resumo: The structural alterations that occur in the muscle fibers of denervated rat diaphragms were studied. Fifteen adult male albino rats (Rattus norvegicus) with a mean weight of 200 g and about 60 days of age were used. Chronically denervated diaphragms were obtained and the animals were sacrificed after 4, 8 and 12 weeks of denervation. The left antimere of the diaphragm was denervated by sectioning of the phrenic nerve and the right antimere served as control. Each antimere was divided into fragments, which were used for analysis transmission electron microscopy. During the initial phase of denervation (4 weeks), ultrastructural muscle modifications appeared in scattered fibers and in foci along these fibers. Muscle fibers with foci of less dense and loosely arranged myofibrils, disorganized Z line, displaced T tubules, and central nucleus exhibiting reentrances and fragmented aspect were observed. After 8 weeks, formation of large aggregates of small elongated mitochondria showing altered cristae, matrix inclusions and increased electron density was noted. At 12 weeks of denervation the alterations were found to be more drastic. Nuclei with internal deposits of myofibrillar or amorphous material were observed. In these fibers, vacuoles harbored enormous myeloid structures in the subsarcolemmal or intermyofibrillar region.
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spelling Ultrastructural Study of the Denervated Diaphragm in RatsDenervationDiaphragmMuscle fiberUltrastructuralRatThe structural alterations that occur in the muscle fibers of denervated rat diaphragms were studied. Fifteen adult male albino rats (Rattus norvegicus) with a mean weight of 200 g and about 60 days of age were used. Chronically denervated diaphragms were obtained and the animals were sacrificed after 4, 8 and 12 weeks of denervation. The left antimere of the diaphragm was denervated by sectioning of the phrenic nerve and the right antimere served as control. Each antimere was divided into fragments, which were used for analysis transmission electron microscopy. During the initial phase of denervation (4 weeks), ultrastructural muscle modifications appeared in scattered fibers and in foci along these fibers. Muscle fibers with foci of less dense and loosely arranged myofibrils, disorganized Z line, displaced T tubules, and central nucleus exhibiting reentrances and fragmented aspect were observed. After 8 weeks, formation of large aggregates of small elongated mitochondria showing altered cristae, matrix inclusions and increased electron density was noted. At 12 weeks of denervation the alterations were found to be more drastic. Nuclei with internal deposits of myofibrillar or amorphous material were observed. In these fibers, vacuoles harbored enormous myeloid structures in the subsarcolemmal or intermyofibrillar region.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Oeste Parana, Med & Pharmaceut Sci Ctr, Cascavel, Parana, BrazilUniv Estadual Paulista, Dept Anat, Biosci Inst, Sao Paulo, BrazilUniv Estadual Maringa, Dept Morphol Sci, Maringa, Parana, BrazilFIB, Sao Paulo, BrazilUniv Estadual Oeste Parana, Cascavel, Parana, BrazilUniv Fed Juiz de Fora, Dept Physiotherapy, Juiz de Fora, MG, BrazilUniv Estadual Paulista, Dept Anat, Biosci Inst, Sao Paulo, BrazilCNPq: 141.412/98-4Soc Chilena AnatomiaUniv Estadual Oeste ParanaUniversidade Estadual Paulista (Unesp)Universidade Estadual de Maringá (UEM)FIBUniversidade Federal de Juiz de Fora (UFJF)Torrejais, M. M.Soares, J. C. [UNESP]Matheus, S. M. M. [UNESP]Mello, J. M.Francia-Farje, L. A. D.Jeronimo, L. C.Vicente, E. J. D.2014-12-03T13:08:41Z2014-12-03T13:08:41Z2013-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article925-931application/pdfhttp://dx.doi.org/10.4067/S0717-95022013000300025International Journal of Morphology. Temuco: Soc Chilena Anatomia, v. 31, n. 3, p. 925-931, 2013.0717-9502http://hdl.handle.net/11449/111487S0717-95022013000300025WOS:000327821700025S0717-95022013000300025.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Morphology0.3360,207info:eu-repo/semantics/openAccess2024-01-23T07:09:51Zoai:repositorio.unesp.br:11449/111487Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:46:37.215067Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Ultrastructural Study of the Denervated Diaphragm in Rats
title Ultrastructural Study of the Denervated Diaphragm in Rats
spellingShingle Ultrastructural Study of the Denervated Diaphragm in Rats
Torrejais, M. M.
Denervation
Diaphragm
Muscle fiber
Ultrastructural
Rat
title_short Ultrastructural Study of the Denervated Diaphragm in Rats
title_full Ultrastructural Study of the Denervated Diaphragm in Rats
title_fullStr Ultrastructural Study of the Denervated Diaphragm in Rats
title_full_unstemmed Ultrastructural Study of the Denervated Diaphragm in Rats
title_sort Ultrastructural Study of the Denervated Diaphragm in Rats
author Torrejais, M. M.
author_facet Torrejais, M. M.
Soares, J. C. [UNESP]
Matheus, S. M. M. [UNESP]
Mello, J. M.
Francia-Farje, L. A. D.
Jeronimo, L. C.
Vicente, E. J. D.
author_role author
author2 Soares, J. C. [UNESP]
Matheus, S. M. M. [UNESP]
Mello, J. M.
Francia-Farje, L. A. D.
Jeronimo, L. C.
Vicente, E. J. D.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Estadual Oeste Parana
Universidade Estadual Paulista (Unesp)
Universidade Estadual de Maringá (UEM)
FIB
Universidade Federal de Juiz de Fora (UFJF)
dc.contributor.author.fl_str_mv Torrejais, M. M.
Soares, J. C. [UNESP]
Matheus, S. M. M. [UNESP]
Mello, J. M.
Francia-Farje, L. A. D.
Jeronimo, L. C.
Vicente, E. J. D.
dc.subject.por.fl_str_mv Denervation
Diaphragm
Muscle fiber
Ultrastructural
Rat
topic Denervation
Diaphragm
Muscle fiber
Ultrastructural
Rat
description The structural alterations that occur in the muscle fibers of denervated rat diaphragms were studied. Fifteen adult male albino rats (Rattus norvegicus) with a mean weight of 200 g and about 60 days of age were used. Chronically denervated diaphragms were obtained and the animals were sacrificed after 4, 8 and 12 weeks of denervation. The left antimere of the diaphragm was denervated by sectioning of the phrenic nerve and the right antimere served as control. Each antimere was divided into fragments, which were used for analysis transmission electron microscopy. During the initial phase of denervation (4 weeks), ultrastructural muscle modifications appeared in scattered fibers and in foci along these fibers. Muscle fibers with foci of less dense and loosely arranged myofibrils, disorganized Z line, displaced T tubules, and central nucleus exhibiting reentrances and fragmented aspect were observed. After 8 weeks, formation of large aggregates of small elongated mitochondria showing altered cristae, matrix inclusions and increased electron density was noted. At 12 weeks of denervation the alterations were found to be more drastic. Nuclei with internal deposits of myofibrillar or amorphous material were observed. In these fibers, vacuoles harbored enormous myeloid structures in the subsarcolemmal or intermyofibrillar region.
publishDate 2013
dc.date.none.fl_str_mv 2013-09-01
2014-12-03T13:08:41Z
2014-12-03T13:08:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4067/S0717-95022013000300025
International Journal of Morphology. Temuco: Soc Chilena Anatomia, v. 31, n. 3, p. 925-931, 2013.
0717-9502
http://hdl.handle.net/11449/111487
S0717-95022013000300025
WOS:000327821700025
S0717-95022013000300025.pdf
url http://dx.doi.org/10.4067/S0717-95022013000300025
http://hdl.handle.net/11449/111487
identifier_str_mv International Journal of Morphology. Temuco: Soc Chilena Anatomia, v. 31, n. 3, p. 925-931, 2013.
0717-9502
S0717-95022013000300025
WOS:000327821700025
S0717-95022013000300025.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Morphology
0.336
0,207
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 925-931
application/pdf
dc.publisher.none.fl_str_mv Soc Chilena Anatomia
publisher.none.fl_str_mv Soc Chilena Anatomia
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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