Traceability of animal protein byproducts in ruminants by multivariate analysis of isotope ratio mass spectrometry to prevent transmission of prion diseases
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-9199-JVATITD-1487-18 http://hdl.handle.net/11449/185718 |
Resumo: | Background: Ruminant feed containing animal byproduct proteins (ABPs) is prohibited in many countries due to its risk of transmitting prion diseases (PD). In most cases the entire herd is sacrificed, which causes great harm to the producer countries by preventing their exportation of ruminant derived-products. Methods: We used stable isotope ratio mass spectrometry (IRMS) of carbon (C-13/C-12) and nitrogen (N-15/N-14) to trace the animal protein in the blood of 15 buffaloes (Bubalus bubalis) divided into three experimental groups: 1 - received only vegetable protein (VP) during 117 days; 2 - received animal and vegetable protein (AVP); and 3 - received animal and vegetable protein with animal protein subsequently removed (AVPR). Groups 2 and 3 received diets containing 13.7% bovine meat and bone meal (MBM) added to a vegetable diet (from days 21-117 in the AVP group and until day 47 in the AVPR group, when MBM was removed). Results: On the 36th day, differences were detectable in the feeding profile (p < 0.01) among the three experimental groups, which remained for a further 49 days (85th day). The AVPR group showed isotopic rate reversibility on the 110th day by presenting values similar to those in the control group (VP) (p > 0.05), indicating that it took 63 days to eliminate MBM in this group. Total atoms exchange (> 95%) of C-13 and N-15 was observed through incorporation of the diet into the AVP and AVPR groups. Conclusions: IRMS is an accurate and sensitive technique for tracing the feeding profile of ruminants through blood analysis, thus enabling investigation of ABP use. |
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Repositório Institucional da UNESP |
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2946 |
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Traceability of animal protein byproducts in ruminants by multivariate analysis of isotope ratio mass spectrometry to prevent transmission of prion diseasesprion diseasesmonitoringbovine spongiformencephalopathystable isotopesBackground: Ruminant feed containing animal byproduct proteins (ABPs) is prohibited in many countries due to its risk of transmitting prion diseases (PD). In most cases the entire herd is sacrificed, which causes great harm to the producer countries by preventing their exportation of ruminant derived-products. Methods: We used stable isotope ratio mass spectrometry (IRMS) of carbon (C-13/C-12) and nitrogen (N-15/N-14) to trace the animal protein in the blood of 15 buffaloes (Bubalus bubalis) divided into three experimental groups: 1 - received only vegetable protein (VP) during 117 days; 2 - received animal and vegetable protein (AVP); and 3 - received animal and vegetable protein with animal protein subsequently removed (AVPR). Groups 2 and 3 received diets containing 13.7% bovine meat and bone meal (MBM) added to a vegetable diet (from days 21-117 in the AVP group and until day 47 in the AVPR group, when MBM was removed). Results: On the 36th day, differences were detectable in the feeding profile (p < 0.01) among the three experimental groups, which remained for a further 49 days (85th day). The AVPR group showed isotopic rate reversibility on the 110th day by presenting values similar to those in the control group (VP) (p > 0.05), indicating that it took 63 days to eliminate MBM in this group. Total atoms exchange (> 95%) of C-13 and N-15 was observed through incorporation of the diet into the AVP and AVPR groups. Conclusions: IRMS is an accurate and sensitive technique for tracing the feeding profile of ruminants through blood analysis, thus enabling investigation of ABP use.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNESP Sao Paulo State Univ, Ctr Study Venoms & Venomous Anim CEVAP, Botucatu, SP, BrazilUNESP Sao Paulo State Univ, Botucatu Med Sch, Botucatu, SP, BrazilUNESP Sao Paulo State Univ, Coll Agr Sci FCA, Botucatu, SP, BrazilUNESP Sao Paulo State Univ, Stable Isotopes Ctr CIE, Botucatu, SP, BrazilUNESP Sao Paulo State Univ, Coll Vet Med & Anim Husb FMVZ, Botucatu, SP, BrazilUniv Calif Los Angeles, David Geffen Sch Med, Dept Neurol, Los Angeles, CA 90095 USAUNESP Sao Paulo State Univ, Ctr Study Venoms & Venomous Anim CEVAP, Botucatu, SP, BrazilUNESP Sao Paulo State Univ, Botucatu Med Sch, Botucatu, SP, BrazilUNESP Sao Paulo State Univ, Coll Agr Sci FCA, Botucatu, SP, BrazilUNESP Sao Paulo State Univ, Stable Isotopes Ctr CIE, Botucatu, SP, BrazilUNESP Sao Paulo State Univ, Coll Vet Med & Anim Husb FMVZ, Botucatu, SP, BrazilCAPES: 23038.000823/2011-74FAPESP: 2008/57411-4CAPES: 0722/2017CAPES: 88881.142062/2017-01CAPES: 404770/2018-5CAPES: 3/2016CAPES: 18/2018BmcUniversidade Estadual Paulista (Unesp)Univ Calif Los AngelesFerreira, Rui Seabra [UNESP]Fossato da Silva, Daniela Alessandra [UNESP]Biscola, Natalia Perussi [UNESP]Pereira Sartori, Maria Marcia [UNESP]Denadai, Juliana Celia [UNESP]Jorge, Andre Mendes [UNESP]Santos, Lucilene Delazari dos [UNESP]Barraviera, Benedito [UNESP]2019-10-04T12:37:56Z2019-10-04T12:37:56Z2019-05-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://dx.doi.org/10.1590/1678-9199-JVATITD-1487-18Journal Of Venomous Animals And Toxins Including Tropical Diseases. London: Bmc, v. 25, 8 p., 2019.1678-9199http://hdl.handle.net/11449/18571810.1590/1678-9199-JVATITD-1487-18S1678-91992019000100312WOS:000468088500001S1678-91992019000100312.pdf3368404126695911Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Venomous Animals And Toxins Including Tropical Diseasesinfo:eu-repo/semantics/openAccess2024-04-11T15:28:26Zoai:repositorio.unesp.br:11449/185718Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:21:37.023728Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Traceability of animal protein byproducts in ruminants by multivariate analysis of isotope ratio mass spectrometry to prevent transmission of prion diseases |
title |
Traceability of animal protein byproducts in ruminants by multivariate analysis of isotope ratio mass spectrometry to prevent transmission of prion diseases |
spellingShingle |
Traceability of animal protein byproducts in ruminants by multivariate analysis of isotope ratio mass spectrometry to prevent transmission of prion diseases Ferreira, Rui Seabra [UNESP] prion diseases monitoring bovine spongiform encephalopathy stable isotopes |
title_short |
Traceability of animal protein byproducts in ruminants by multivariate analysis of isotope ratio mass spectrometry to prevent transmission of prion diseases |
title_full |
Traceability of animal protein byproducts in ruminants by multivariate analysis of isotope ratio mass spectrometry to prevent transmission of prion diseases |
title_fullStr |
Traceability of animal protein byproducts in ruminants by multivariate analysis of isotope ratio mass spectrometry to prevent transmission of prion diseases |
title_full_unstemmed |
Traceability of animal protein byproducts in ruminants by multivariate analysis of isotope ratio mass spectrometry to prevent transmission of prion diseases |
title_sort |
Traceability of animal protein byproducts in ruminants by multivariate analysis of isotope ratio mass spectrometry to prevent transmission of prion diseases |
author |
Ferreira, Rui Seabra [UNESP] |
author_facet |
Ferreira, Rui Seabra [UNESP] Fossato da Silva, Daniela Alessandra [UNESP] Biscola, Natalia Perussi [UNESP] Pereira Sartori, Maria Marcia [UNESP] Denadai, Juliana Celia [UNESP] Jorge, Andre Mendes [UNESP] Santos, Lucilene Delazari dos [UNESP] Barraviera, Benedito [UNESP] |
author_role |
author |
author2 |
Fossato da Silva, Daniela Alessandra [UNESP] Biscola, Natalia Perussi [UNESP] Pereira Sartori, Maria Marcia [UNESP] Denadai, Juliana Celia [UNESP] Jorge, Andre Mendes [UNESP] Santos, Lucilene Delazari dos [UNESP] Barraviera, Benedito [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Calif Los Angeles |
dc.contributor.author.fl_str_mv |
Ferreira, Rui Seabra [UNESP] Fossato da Silva, Daniela Alessandra [UNESP] Biscola, Natalia Perussi [UNESP] Pereira Sartori, Maria Marcia [UNESP] Denadai, Juliana Celia [UNESP] Jorge, Andre Mendes [UNESP] Santos, Lucilene Delazari dos [UNESP] Barraviera, Benedito [UNESP] |
dc.subject.por.fl_str_mv |
prion diseases monitoring bovine spongiform encephalopathy stable isotopes |
topic |
prion diseases monitoring bovine spongiform encephalopathy stable isotopes |
description |
Background: Ruminant feed containing animal byproduct proteins (ABPs) is prohibited in many countries due to its risk of transmitting prion diseases (PD). In most cases the entire herd is sacrificed, which causes great harm to the producer countries by preventing their exportation of ruminant derived-products. Methods: We used stable isotope ratio mass spectrometry (IRMS) of carbon (C-13/C-12) and nitrogen (N-15/N-14) to trace the animal protein in the blood of 15 buffaloes (Bubalus bubalis) divided into three experimental groups: 1 - received only vegetable protein (VP) during 117 days; 2 - received animal and vegetable protein (AVP); and 3 - received animal and vegetable protein with animal protein subsequently removed (AVPR). Groups 2 and 3 received diets containing 13.7% bovine meat and bone meal (MBM) added to a vegetable diet (from days 21-117 in the AVP group and until day 47 in the AVPR group, when MBM was removed). Results: On the 36th day, differences were detectable in the feeding profile (p < 0.01) among the three experimental groups, which remained for a further 49 days (85th day). The AVPR group showed isotopic rate reversibility on the 110th day by presenting values similar to those in the control group (VP) (p > 0.05), indicating that it took 63 days to eliminate MBM in this group. Total atoms exchange (> 95%) of C-13 and N-15 was observed through incorporation of the diet into the AVP and AVPR groups. Conclusions: IRMS is an accurate and sensitive technique for tracing the feeding profile of ruminants through blood analysis, thus enabling investigation of ABP use. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T12:37:56Z 2019-10-04T12:37:56Z 2019-05-13 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-9199-JVATITD-1487-18 Journal Of Venomous Animals And Toxins Including Tropical Diseases. London: Bmc, v. 25, 8 p., 2019. 1678-9199 http://hdl.handle.net/11449/185718 10.1590/1678-9199-JVATITD-1487-18 S1678-91992019000100312 WOS:000468088500001 S1678-91992019000100312.pdf 3368404126695911 |
url |
http://dx.doi.org/10.1590/1678-9199-JVATITD-1487-18 http://hdl.handle.net/11449/185718 |
identifier_str_mv |
Journal Of Venomous Animals And Toxins Including Tropical Diseases. London: Bmc, v. 25, 8 p., 2019. 1678-9199 10.1590/1678-9199-JVATITD-1487-18 S1678-91992019000100312 WOS:000468088500001 S1678-91992019000100312.pdf 3368404126695911 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Venomous Animals And Toxins Including Tropical Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 application/pdf |
dc.publisher.none.fl_str_mv |
Bmc |
publisher.none.fl_str_mv |
Bmc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129058028912640 |