Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms

Detalhes bibliográficos
Autor(a) principal: Rossoni, Rodnei Dennis [UNESP]
Data de Publicação: 2018
Outros Autores: Velloso, Marisol dos Santos [UNESP], de Barros, Patrícia Pimentel [UNESP], de Alvarenga, Janaina Araújo [UNESP], Santos, Jéssica Diane dos [UNESP], Santos Prado, Ana Carolina Chipoletti dos [UNESP], Ribeiro, Felipe de Camargo [UNESP], Anbinder, Ana Lia [UNESP], Junqueira, Juliana Campos [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.micpath.2018.07.032
http://hdl.handle.net/11449/176638
Resumo: Probiotics can release bioactive substances that can inhibit the growth and biofilm formation of pathogenic microorganisms such as Streptococcus mutans. In this context, we evaluated whether the supernatants of Lactobacillus strains isolated from caries-free subjects can inhibit S. mutans, one of the most important bacteria for dental caries. First, the supernatants of 22 Lactobacillus strains were screened for antibacterial activity against S. mutans in planktonic cultures. All 22 Lactobacillus strains studied (100%) showed antibacterial activity. Thereafter, the Lactobacillus strains with the greatest reductions in the planktonic S. mutans cultures were tested on biofilms. The L. fermentum 20.4, L. paracasei 11.6, L. paracasei 20.3 and L. paracasei 25.4 strains could significantly reduce the number of S. mutans cells in biofilms formed in hydroxyapatite (p < 0.05). This reduction was also confirmed by scanning electron microscopy analysis and was not caused by the decreased pH value in the medium (p > 0.05). In addition, the supernatants of these probiotic strains could also reduce the total biomass of S. mutans biofilms (p < 0.05). In conclusion, most of the Lactobacillus strains tested have some antibacterial activity against S. mutans. L. fermentum 20.4, L. paracasei 11.6, L. paracasei 20.3 and L. paracasei 25.4 produce bioactive substances that caused a significant reduction in S. mutans biofilms.
id UNSP_d9500686fd35f31e82be6fc1feab3bf2
oai_identifier_str oai:repositorio.unesp.br:11449/176638
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilmsBiofilmLactobacillusOral pathogensStreptococcus mutansSupernatantProbiotics can release bioactive substances that can inhibit the growth and biofilm formation of pathogenic microorganisms such as Streptococcus mutans. In this context, we evaluated whether the supernatants of Lactobacillus strains isolated from caries-free subjects can inhibit S. mutans, one of the most important bacteria for dental caries. First, the supernatants of 22 Lactobacillus strains were screened for antibacterial activity against S. mutans in planktonic cultures. All 22 Lactobacillus strains studied (100%) showed antibacterial activity. Thereafter, the Lactobacillus strains with the greatest reductions in the planktonic S. mutans cultures were tested on biofilms. The L. fermentum 20.4, L. paracasei 11.6, L. paracasei 20.3 and L. paracasei 25.4 strains could significantly reduce the number of S. mutans cells in biofilms formed in hydroxyapatite (p < 0.05). This reduction was also confirmed by scanning electron microscopy analysis and was not caused by the decreased pH value in the medium (p > 0.05). In addition, the supernatants of these probiotic strains could also reduce the total biomass of S. mutans biofilms (p < 0.05). In conclusion, most of the Lactobacillus strains tested have some antibacterial activity against S. mutans. L. fermentum 20.4, L. paracasei 11.6, L. paracasei 20.3 and L. paracasei 25.4 produce bioactive substances that caused a significant reduction in S. mutans biofilms.Department of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology, São José dos CamposDepartment of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology, São José dos CamposUniversidade Estadual Paulista (Unesp)Rossoni, Rodnei Dennis [UNESP]Velloso, Marisol dos Santos [UNESP]de Barros, Patrícia Pimentel [UNESP]de Alvarenga, Janaina Araújo [UNESP]Santos, Jéssica Diane dos [UNESP]Santos Prado, Ana Carolina Chipoletti dos [UNESP]Ribeiro, Felipe de Camargo [UNESP]Anbinder, Ana Lia [UNESP]Junqueira, Juliana Campos [UNESP]2018-12-11T17:21:51Z2018-12-11T17:21:51Z2018-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article361-367application/pdfhttp://dx.doi.org/10.1016/j.micpath.2018.07.032Microbial Pathogenesis, v. 123, p. 361-367.1096-12080882-4010http://hdl.handle.net/11449/17663810.1016/j.micpath.2018.07.0322-s2.0-850505075662-s2.0-85050507566.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicrobial Pathogenesis0,7510,751info:eu-repo/semantics/openAccess2023-11-07T06:12:02Zoai:repositorio.unesp.br:11449/176638Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:05:11.425413Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms
title Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms
spellingShingle Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms
Rossoni, Rodnei Dennis [UNESP]
Biofilm
Lactobacillus
Oral pathogens
Streptococcus mutans
Supernatant
title_short Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms
title_full Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms
title_fullStr Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms
title_full_unstemmed Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms
title_sort Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms
author Rossoni, Rodnei Dennis [UNESP]
author_facet Rossoni, Rodnei Dennis [UNESP]
Velloso, Marisol dos Santos [UNESP]
de Barros, Patrícia Pimentel [UNESP]
de Alvarenga, Janaina Araújo [UNESP]
Santos, Jéssica Diane dos [UNESP]
Santos Prado, Ana Carolina Chipoletti dos [UNESP]
Ribeiro, Felipe de Camargo [UNESP]
Anbinder, Ana Lia [UNESP]
Junqueira, Juliana Campos [UNESP]
author_role author
author2 Velloso, Marisol dos Santos [UNESP]
de Barros, Patrícia Pimentel [UNESP]
de Alvarenga, Janaina Araújo [UNESP]
Santos, Jéssica Diane dos [UNESP]
Santos Prado, Ana Carolina Chipoletti dos [UNESP]
Ribeiro, Felipe de Camargo [UNESP]
Anbinder, Ana Lia [UNESP]
Junqueira, Juliana Campos [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Rossoni, Rodnei Dennis [UNESP]
Velloso, Marisol dos Santos [UNESP]
de Barros, Patrícia Pimentel [UNESP]
de Alvarenga, Janaina Araújo [UNESP]
Santos, Jéssica Diane dos [UNESP]
Santos Prado, Ana Carolina Chipoletti dos [UNESP]
Ribeiro, Felipe de Camargo [UNESP]
Anbinder, Ana Lia [UNESP]
Junqueira, Juliana Campos [UNESP]
dc.subject.por.fl_str_mv Biofilm
Lactobacillus
Oral pathogens
Streptococcus mutans
Supernatant
topic Biofilm
Lactobacillus
Oral pathogens
Streptococcus mutans
Supernatant
description Probiotics can release bioactive substances that can inhibit the growth and biofilm formation of pathogenic microorganisms such as Streptococcus mutans. In this context, we evaluated whether the supernatants of Lactobacillus strains isolated from caries-free subjects can inhibit S. mutans, one of the most important bacteria for dental caries. First, the supernatants of 22 Lactobacillus strains were screened for antibacterial activity against S. mutans in planktonic cultures. All 22 Lactobacillus strains studied (100%) showed antibacterial activity. Thereafter, the Lactobacillus strains with the greatest reductions in the planktonic S. mutans cultures were tested on biofilms. The L. fermentum 20.4, L. paracasei 11.6, L. paracasei 20.3 and L. paracasei 25.4 strains could significantly reduce the number of S. mutans cells in biofilms formed in hydroxyapatite (p < 0.05). This reduction was also confirmed by scanning electron microscopy analysis and was not caused by the decreased pH value in the medium (p > 0.05). In addition, the supernatants of these probiotic strains could also reduce the total biomass of S. mutans biofilms (p < 0.05). In conclusion, most of the Lactobacillus strains tested have some antibacterial activity against S. mutans. L. fermentum 20.4, L. paracasei 11.6, L. paracasei 20.3 and L. paracasei 25.4 produce bioactive substances that caused a significant reduction in S. mutans biofilms.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:21:51Z
2018-12-11T17:21:51Z
2018-10-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.micpath.2018.07.032
Microbial Pathogenesis, v. 123, p. 361-367.
1096-1208
0882-4010
http://hdl.handle.net/11449/176638
10.1016/j.micpath.2018.07.032
2-s2.0-85050507566
2-s2.0-85050507566.pdf
url http://dx.doi.org/10.1016/j.micpath.2018.07.032
http://hdl.handle.net/11449/176638
identifier_str_mv Microbial Pathogenesis, v. 123, p. 361-367.
1096-1208
0882-4010
10.1016/j.micpath.2018.07.032
2-s2.0-85050507566
2-s2.0-85050507566.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microbial Pathogenesis
0,751
0,751
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 361-367
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128753650368512