Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.molliq.2020.113324 http://hdl.handle.net/11449/201764 |
Resumo: | Risks to human health have been reported owing to prolonged exposure to hormones, whose action depends on their molecular-level interaction with cell membranes. In this study, we investigate the interaction of the synthetic hormone 17 α-ethynylestradiol (EE2) in two different membrane models, Langmuir monolayers and giant unilamellar vesicles (GUVs) made with a binary mixture of 1,2-dipalmitoyl-sn-glycerol-3-phosphocholine (DPPC) and cholesterol (Chol) in order to mimic the plasma membrane of mammalian cells. EE2 was found to expand the Langmuir monolayers, with shifts to larger areas per molecule in the surface pressure isotherm. In all of these observations, stronger effects were noted for the DPPC/Chol monolayers with XChol = 0.3, which mimics the proportion of phospholipid/sterol in the plasma membrane. At high surface pressures, EE2 is believed to weaken the attractive interactions between DPPC and Chol, in addition to affecting the ordering of the lipid chains as indicated in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) measurements. In GUVs obtained with XChol = 0.3 mixtures, EE2 induced a phase contrast loss as a result of increased permeability of the lipid bilayer. The results with Langmuir monolayers and GUV combined point to EE2 action on representative cell membranes, which can be correlated with physiological effects caused by indirect intake of EE2. |
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Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol17α-EthynylestradiolCholesterolDPPCGiant unilamellar vesiclesLangmuir monolayersMembrane modelPM-IRRASRisks to human health have been reported owing to prolonged exposure to hormones, whose action depends on their molecular-level interaction with cell membranes. In this study, we investigate the interaction of the synthetic hormone 17 α-ethynylestradiol (EE2) in two different membrane models, Langmuir monolayers and giant unilamellar vesicles (GUVs) made with a binary mixture of 1,2-dipalmitoyl-sn-glycerol-3-phosphocholine (DPPC) and cholesterol (Chol) in order to mimic the plasma membrane of mammalian cells. EE2 was found to expand the Langmuir monolayers, with shifts to larger areas per molecule in the surface pressure isotherm. In all of these observations, stronger effects were noted for the DPPC/Chol monolayers with XChol = 0.3, which mimics the proportion of phospholipid/sterol in the plasma membrane. At high surface pressures, EE2 is believed to weaken the attractive interactions between DPPC and Chol, in addition to affecting the ordering of the lipid chains as indicated in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) measurements. In GUVs obtained with XChol = 0.3 mixtures, EE2 induced a phase contrast loss as a result of increased permeability of the lipid bilayer. The results with Langmuir monolayers and GUV combined point to EE2 action on representative cell membranes, which can be correlated with physiological effects caused by indirect intake of EE2.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Instituto Nacional de Ciência e Tecnologia em Eletrônica OrgânicaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Physics School of Technology and Applied Sciences São Paulo State University (UNESP)Sao Carlos Institute of Physics University of Sao Paulo (USP)Department of Physics School of Technology and Applied Sciences São Paulo State University (UNESP)FAPESP: 2013/14262-7FAPESP: 2016/09633-4CAPES: 88882.330129/2018-01Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Ruiz, Gilia Cristine Marques [UNESP]Pazin, Wallance Moreira [UNESP]do Carmo Morato, Luis Fernando [UNESP]Oliveira, Osvaldo N.Constantino, Carlos José Leopoldo [UNESP]2020-12-12T02:41:14Z2020-12-12T02:41:14Z2020-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.molliq.2020.113324Journal of Molecular Liquids, v. 311.0167-7322http://hdl.handle.net/11449/20176410.1016/j.molliq.2020.1133242-s2.0-85084862756Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Molecular Liquidsinfo:eu-repo/semantics/openAccess2024-06-19T12:44:24Zoai:repositorio.unesp.br:11449/201764Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:03:34.150678Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol |
title |
Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol |
spellingShingle |
Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol Ruiz, Gilia Cristine Marques [UNESP] 17α-Ethynylestradiol Cholesterol DPPC Giant unilamellar vesicles Langmuir monolayers Membrane model PM-IRRAS |
title_short |
Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol |
title_full |
Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol |
title_fullStr |
Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol |
title_full_unstemmed |
Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol |
title_sort |
Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol |
author |
Ruiz, Gilia Cristine Marques [UNESP] |
author_facet |
Ruiz, Gilia Cristine Marques [UNESP] Pazin, Wallance Moreira [UNESP] do Carmo Morato, Luis Fernando [UNESP] Oliveira, Osvaldo N. Constantino, Carlos José Leopoldo [UNESP] |
author_role |
author |
author2 |
Pazin, Wallance Moreira [UNESP] do Carmo Morato, Luis Fernando [UNESP] Oliveira, Osvaldo N. Constantino, Carlos José Leopoldo [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Ruiz, Gilia Cristine Marques [UNESP] Pazin, Wallance Moreira [UNESP] do Carmo Morato, Luis Fernando [UNESP] Oliveira, Osvaldo N. Constantino, Carlos José Leopoldo [UNESP] |
dc.subject.por.fl_str_mv |
17α-Ethynylestradiol Cholesterol DPPC Giant unilamellar vesicles Langmuir monolayers Membrane model PM-IRRAS |
topic |
17α-Ethynylestradiol Cholesterol DPPC Giant unilamellar vesicles Langmuir monolayers Membrane model PM-IRRAS |
description |
Risks to human health have been reported owing to prolonged exposure to hormones, whose action depends on their molecular-level interaction with cell membranes. In this study, we investigate the interaction of the synthetic hormone 17 α-ethynylestradiol (EE2) in two different membrane models, Langmuir monolayers and giant unilamellar vesicles (GUVs) made with a binary mixture of 1,2-dipalmitoyl-sn-glycerol-3-phosphocholine (DPPC) and cholesterol (Chol) in order to mimic the plasma membrane of mammalian cells. EE2 was found to expand the Langmuir monolayers, with shifts to larger areas per molecule in the surface pressure isotherm. In all of these observations, stronger effects were noted for the DPPC/Chol monolayers with XChol = 0.3, which mimics the proportion of phospholipid/sterol in the plasma membrane. At high surface pressures, EE2 is believed to weaken the attractive interactions between DPPC and Chol, in addition to affecting the ordering of the lipid chains as indicated in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) measurements. In GUVs obtained with XChol = 0.3 mixtures, EE2 induced a phase contrast loss as a result of increased permeability of the lipid bilayer. The results with Langmuir monolayers and GUV combined point to EE2 action on representative cell membranes, which can be correlated with physiological effects caused by indirect intake of EE2. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:41:14Z 2020-12-12T02:41:14Z 2020-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.molliq.2020.113324 Journal of Molecular Liquids, v. 311. 0167-7322 http://hdl.handle.net/11449/201764 10.1016/j.molliq.2020.113324 2-s2.0-85084862756 |
url |
http://dx.doi.org/10.1016/j.molliq.2020.113324 http://hdl.handle.net/11449/201764 |
identifier_str_mv |
Journal of Molecular Liquids, v. 311. 0167-7322 10.1016/j.molliq.2020.113324 2-s2.0-85084862756 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Molecular Liquids |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128747753177088 |