Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol

Detalhes bibliográficos
Autor(a) principal: Ruiz, Gilia Cristine Marques [UNESP]
Data de Publicação: 2020
Outros Autores: Pazin, Wallance Moreira [UNESP], do Carmo Morato, Luis Fernando [UNESP], Oliveira, Osvaldo N., Constantino, Carlos José Leopoldo [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.molliq.2020.113324
http://hdl.handle.net/11449/201764
Resumo: Risks to human health have been reported owing to prolonged exposure to hormones, whose action depends on their molecular-level interaction with cell membranes. In this study, we investigate the interaction of the synthetic hormone 17 α-ethynylestradiol (EE2) in two different membrane models, Langmuir monolayers and giant unilamellar vesicles (GUVs) made with a binary mixture of 1,2-dipalmitoyl-sn-glycerol-3-phosphocholine (DPPC) and cholesterol (Chol) in order to mimic the plasma membrane of mammalian cells. EE2 was found to expand the Langmuir monolayers, with shifts to larger areas per molecule in the surface pressure isotherm. In all of these observations, stronger effects were noted for the DPPC/Chol monolayers with XChol = 0.3, which mimics the proportion of phospholipid/sterol in the plasma membrane. At high surface pressures, EE2 is believed to weaken the attractive interactions between DPPC and Chol, in addition to affecting the ordering of the lipid chains as indicated in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) measurements. In GUVs obtained with XChol = 0.3 mixtures, EE2 induced a phase contrast loss as a result of increased permeability of the lipid bilayer. The results with Langmuir monolayers and GUV combined point to EE2 action on representative cell membranes, which can be correlated with physiological effects caused by indirect intake of EE2.
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spelling Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol17α-EthynylestradiolCholesterolDPPCGiant unilamellar vesiclesLangmuir monolayersMembrane modelPM-IRRASRisks to human health have been reported owing to prolonged exposure to hormones, whose action depends on their molecular-level interaction with cell membranes. In this study, we investigate the interaction of the synthetic hormone 17 α-ethynylestradiol (EE2) in two different membrane models, Langmuir monolayers and giant unilamellar vesicles (GUVs) made with a binary mixture of 1,2-dipalmitoyl-sn-glycerol-3-phosphocholine (DPPC) and cholesterol (Chol) in order to mimic the plasma membrane of mammalian cells. EE2 was found to expand the Langmuir monolayers, with shifts to larger areas per molecule in the surface pressure isotherm. In all of these observations, stronger effects were noted for the DPPC/Chol monolayers with XChol = 0.3, which mimics the proportion of phospholipid/sterol in the plasma membrane. At high surface pressures, EE2 is believed to weaken the attractive interactions between DPPC and Chol, in addition to affecting the ordering of the lipid chains as indicated in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) measurements. In GUVs obtained with XChol = 0.3 mixtures, EE2 induced a phase contrast loss as a result of increased permeability of the lipid bilayer. The results with Langmuir monolayers and GUV combined point to EE2 action on representative cell membranes, which can be correlated with physiological effects caused by indirect intake of EE2.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Instituto Nacional de Ciência e Tecnologia em Eletrônica OrgânicaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Physics School of Technology and Applied Sciences São Paulo State University (UNESP)Sao Carlos Institute of Physics University of Sao Paulo (USP)Department of Physics School of Technology and Applied Sciences São Paulo State University (UNESP)FAPESP: 2013/14262-7FAPESP: 2016/09633-4CAPES: 88882.330129/2018-01Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Ruiz, Gilia Cristine Marques [UNESP]Pazin, Wallance Moreira [UNESP]do Carmo Morato, Luis Fernando [UNESP]Oliveira, Osvaldo N.Constantino, Carlos José Leopoldo [UNESP]2020-12-12T02:41:14Z2020-12-12T02:41:14Z2020-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.molliq.2020.113324Journal of Molecular Liquids, v. 311.0167-7322http://hdl.handle.net/11449/20176410.1016/j.molliq.2020.1133242-s2.0-85084862756Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Molecular Liquidsinfo:eu-repo/semantics/openAccess2024-06-19T12:44:24Zoai:repositorio.unesp.br:11449/201764Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:03:34.150678Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol
title Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol
spellingShingle Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol
Ruiz, Gilia Cristine Marques [UNESP]
17α-Ethynylestradiol
Cholesterol
DPPC
Giant unilamellar vesicles
Langmuir monolayers
Membrane model
PM-IRRAS
title_short Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol
title_full Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol
title_fullStr Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol
title_full_unstemmed Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol
title_sort Correlating mono- and bilayers of lipids to investigate the pronounced effects of steroid hormone 17α-ethynylestradiol on membrane models of DPPC/cholesterol
author Ruiz, Gilia Cristine Marques [UNESP]
author_facet Ruiz, Gilia Cristine Marques [UNESP]
Pazin, Wallance Moreira [UNESP]
do Carmo Morato, Luis Fernando [UNESP]
Oliveira, Osvaldo N.
Constantino, Carlos José Leopoldo [UNESP]
author_role author
author2 Pazin, Wallance Moreira [UNESP]
do Carmo Morato, Luis Fernando [UNESP]
Oliveira, Osvaldo N.
Constantino, Carlos José Leopoldo [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Ruiz, Gilia Cristine Marques [UNESP]
Pazin, Wallance Moreira [UNESP]
do Carmo Morato, Luis Fernando [UNESP]
Oliveira, Osvaldo N.
Constantino, Carlos José Leopoldo [UNESP]
dc.subject.por.fl_str_mv 17α-Ethynylestradiol
Cholesterol
DPPC
Giant unilamellar vesicles
Langmuir monolayers
Membrane model
PM-IRRAS
topic 17α-Ethynylestradiol
Cholesterol
DPPC
Giant unilamellar vesicles
Langmuir monolayers
Membrane model
PM-IRRAS
description Risks to human health have been reported owing to prolonged exposure to hormones, whose action depends on their molecular-level interaction with cell membranes. In this study, we investigate the interaction of the synthetic hormone 17 α-ethynylestradiol (EE2) in two different membrane models, Langmuir monolayers and giant unilamellar vesicles (GUVs) made with a binary mixture of 1,2-dipalmitoyl-sn-glycerol-3-phosphocholine (DPPC) and cholesterol (Chol) in order to mimic the plasma membrane of mammalian cells. EE2 was found to expand the Langmuir monolayers, with shifts to larger areas per molecule in the surface pressure isotherm. In all of these observations, stronger effects were noted for the DPPC/Chol monolayers with XChol = 0.3, which mimics the proportion of phospholipid/sterol in the plasma membrane. At high surface pressures, EE2 is believed to weaken the attractive interactions between DPPC and Chol, in addition to affecting the ordering of the lipid chains as indicated in polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) measurements. In GUVs obtained with XChol = 0.3 mixtures, EE2 induced a phase contrast loss as a result of increased permeability of the lipid bilayer. The results with Langmuir monolayers and GUV combined point to EE2 action on representative cell membranes, which can be correlated with physiological effects caused by indirect intake of EE2.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:41:14Z
2020-12-12T02:41:14Z
2020-08-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.molliq.2020.113324
Journal of Molecular Liquids, v. 311.
0167-7322
http://hdl.handle.net/11449/201764
10.1016/j.molliq.2020.113324
2-s2.0-85084862756
url http://dx.doi.org/10.1016/j.molliq.2020.113324
http://hdl.handle.net/11449/201764
identifier_str_mv Journal of Molecular Liquids, v. 311.
0167-7322
10.1016/j.molliq.2020.113324
2-s2.0-85084862756
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Molecular Liquids
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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